当前位置： SCI文献检索 > NEUROSCIENCE期刊下所有文献 > Effect of glucose on glutamine metabolism in rat brain slices: a cellular metabolomic study with Effect of glucose ¹³C NMR.

Effect of glucose on glutamine metabolism in rat brain slices: a cellular metabolomic study with Effect of glucose ¹³C NMR.

Abstract：

:To examine the effect of glucose on the cerebral metabolism of glutamine, rat brain slices were incubated with 5mM [3-(13)C]glutamine without and with 5mM unlabeled glucose. Tissue plus medium extracts were analyzed by using enzymatic and (13)C NMR techniques and fluxes through the enzymatic steps involved were calculated with a mathematical model. We demonstrate that glucose increased alanine, pyruvate and glutamate accumulations and decreased ammonium ions accumulation, aspartate accumulation and labeling, and GABA labeling. In order to determine the participation of glutamine synthetase when glucose was added to the incubation medium, we incubated rat brain slices with 5mM [3-(13)C]glutamine plus 5mM unlabeled glucose without and with 2mM methionine sulfoximine (MSO). The results indicate that 77% of the newly appeared glutamine was formed via glutamine synthetase and 23% from endogenous sources; the stimulation of [3-(13)C]glutamine removal by MSO also strongly suggests the existence of a cycle between [3-(13)C]glutamine and [3-(13)C]glutamate. This work also demonstrates that glucose increased fluxes through hexokinase, pyruvate kinase, lactate dehydrogenase, alanine aminotransferase, pyruvate carboxylase, pyruvate dehydrogenase, citrate synthase, flux from α-ketoglutarate to glutamate and flux through glutamine synthetase whereas it inhibited fluxes through aspartate aminotransferase, glutamic acid decarboxylase and GABA aminotransferase.

journal_name

Neuroscience

journal_title

Neuroscience

authors

El Hage M,Baverel G,Conjard-Duplany A,Martin G

doi

10.1016/j.neuroscience.2013.05.064

subject

Has Abstract

pub_date

2013-09-17 00:00:00

pages

243-51

eissn

0306-4522

issn

1873-7544

pii

S0306-4522(13)00500-9

journal_volume

248

pub_type

杂志文章

相关文献

NEUROSCIENCE文献大全
  • The glutamate-based genetic immune hypothesis in obsessive-compulsive disorder. An integrative approach from genes to symptoms.

    abstract：:Recent advances in multiple areas of research have contributed to the identification of several pathophysiological factors underlying obsessive-compulsive disorder (OCD). In particular, the glutamate transporter gene SLC1A1 has been associated with the diagnosis of OCD. Immunological and infectious studies have report...

    journal_title：Neuroscience

    pub_type： 杂志文章,评审

    doi：10.1016/j.neuroscience.2009.10.043

    authors： Rotge JY,Aouizerate B,Tignol J,Bioulac B,Burbaud P,Guehl D

    更新日期：2010-01-20 00:00:00

  • A population of large lamina I projection neurons with selective inhibitory input in rat spinal cord.

    abstract：:Lamina I of the spinal dorsal horn contains a diverse mixture of neurons. Among these, a group of giant neurons (Waldeyer cells) has long been recognized. In this study we have used immunocytochemistry to characterize a population of Waldeyer cells which were identified by the presence of high levels of the glycine re...

    journal_title：Neuroscience

    pub_type： 杂志文章

    doi：10.1016/s0306-4522(00)00445-0

    authors： Puskár Z,Polgár E,Todd AJ

    更新日期：2001-01-01 00:00:00

  • Morphological and biochemical adaptations to unilateral dopamine denervation of the neostriatum in newborn rats.

    abstract：:Basal ganglia of adult rats were examined for morphological and biochemical changes resulting from neonatal unilateral dopamine denervation of the striatum with increasing doses of 6-hydroxydopamine (4, 12 and 20 microg). Rotational behaviour induced by apomorphine (0.1 mg/kg) was observed in all rats injected with th...

    journal_title：Neuroscience

    pub_type： 杂志文章

    doi：10.1016/s0306-4522(96)00500-3

    authors： Penit-Soria J,Durand C,Herve D,Besson MJ

    更新日期：1997-04-01 00:00:00

  • Desynchronisation of spontaneously recurrent experimental seizures proceeds with a single rhythm.

    abstract：:Here we investigate the temporal properties of recurrent seizure-like events (SLEs) in a low-[Mg(2+)] model of experimental epilepsy. Simultaneous intra- and extracellular electric signals were recorded in the CA3 region of rat hippocampal slices whereby cytosolic [Ca(2+)] transients were imaged by fluorescence detect...

    journal_title：Neuroscience

    pub_type： 杂志文章

    doi：10.1016/s0306-4522(03)00559-1

    authors： Nyikos L,Lasztóczi B,Antal K,Kovács R,Kardos J

    更新日期：2003-01-01 00:00:00

  • Estradiol influences oxytocin-immunoreactive brain systems.

    abstract：:The rat brain was examined immunocytochemically for estrogen-dependent changes of oxytocin immunoreactivity at the light microscopical level. Ovariectomized rats were treated with subcutaneous silastic implants with estradiol, or empty implants as controls for 2 days (short term treatment). Another group of rats was i...

    journal_title：Neuroscience

    pub_type： 杂志文章

    doi：10.1016/0306-4522(88)90022-x

    authors： Jirikowski GF,Caldwell JD,Pedersen CA,Stumpf WE

    更新日期：1988-04-01 00:00:00

  • β₂-adrenergic receptors protect axons during energetic stress but do not influence basal glio-axonal lactate shuttling in mouse white matter.

    abstract：:In vitro studies have demonstrated that β2-adrenergic receptor activation stimulates glycogen degradation in astrocytes, generating lactate as a potential energy source for neurons. Using in vivo microdialysis in mouse cerebellar white matter we demonstrate continuous axonal lactate uptake and glial-axonal metabolic c...

    journal_title：Neuroscience

    pub_type： 杂志文章

    doi：10.1016/j.neuroscience.2014.07.022

    authors： Laureys G,Valentino M,Demol F,Zammit C,Muscat R,Cambron M,Kooijman R,De Keyser J

    更新日期：2014-09-26 00:00:00

  • AT1 receptor blockade in the central nucleus of the amygdala attenuates the effects of muscimol on sodium and water intake.

    abstract：:The blockade of the central nucleus of the amygdala (CeA) with the GABAA receptor agonist muscimol significantly reduces hypertonic NaCl and water intake by sodium-depleted rats. In the present study we investigated the effects of previous injection of losartan, an angiotensin II type-1 (AT1) receptor antagonist, into...

    journal_title：Neuroscience

    pub_type： 杂志文章

    doi：10.1016/j.neuroscience.2015.08.069

    authors： Hu B,Qiao H,Sun B,Jia R,Fan Y,Wang N,Lu B,Yan JQ

    更新日期：2015-10-29 00:00:00

  • Gene expression profile analysis of epilepsy-associated gangliogliomas.

    abstract：:Gangliogliomas (GG) constitute the most frequent tumor entity in young patients undergoing surgery for intractable epilepsy. The histological composition of GG, with the presence of dysplastic neurons, corroborates their maldevelopmental origin. However, their histogenesis, the pathogenetic relationship with other dev...

    journal_title：Neuroscience

    pub_type： 杂志文章

    doi：10.1016/j.neuroscience.2007.10.036

    authors： Aronica E,Boer K,Becker A,Redeker S,Spliet WG,van Rijen PC,Wittink F,Breit T,Wadman WJ,Lopes da Silva FH,Troost D,Gorter JA

    更新日期：2008-01-02 00:00:00

  • Glycogen synthase kinase 3-specific inhibitor AR-A014418 decreases neuropathic pain in mice: evidence for the mechanisms of action.

    abstract：:The present study examined the antihyperalgesic effect of a specific inhibitor of Glycogen Synthase Kinase 3 (GSK3), AR-A014418, on the partial ligation of the sciatic nerve (PSNL), a neuropathic pain model in mice and investigated some mechanisms of action. AR-A014418 (0.01-1 mg/kg) administered by intraperitoneal ro...

    journal_title：Neuroscience

    pub_type： 杂志文章

    doi：10.1016/j.neuroscience.2012.09.020

    authors： Mazzardo-Martins L,Martins DF,Stramosk J,Cidral-Filho FJ,Santos AR

    更新日期：2012-12-13 00:00:00

  • Activation and Inactivation of Nicotinic Receptnors in the Dorsal Hippocampal Region Restored Negative Effects of Total (TSD) and REM Sleep Deprivation (RSD) on Memory Acquisition, Locomotor Activity and Pain Perception.

    abstract：:Sleep deprivation (SD) is a common issue in today's society. Sleep is essential for proper cognitive functions, including learning and memory. Furthermore, sleep disorders can alter pain information processing. Meanwhile, hippocampal nicotinic receptors have a role in modulating pain and memory. The goal of this study...

    journal_title：Neuroscience

    pub_type： 杂志文章

    doi：10.1016/j.neuroscience.2020.03.006

    authors： Javad-Moosavi BZ,Nasehi M,Vaseghi S,Jamaldini SH,Zarrindast MR

    更新日期：2020-05-01 00:00:00

  • The relationship of the medullary catecholamine containing neurones to the vagal motor nuclei.

    abstract：:We have re-examined in the rat the nuclear localization of the medullary catecholamine-containing cell groups (A1 and A2) and their relation to the vagal motor nuclei using a double labeling method. The vagal nuclei were defined by the retrograde transport of horseradish peroxidase applied to the cervical vagus, and n...

    journal_title：Neuroscience

    pub_type： 杂志文章

    doi：10.1016/0306-4522(82)90258-5

    authors： Ritchie TC,Westlund KN,Bowker RM,Coulter JD,Leonard RB

    更新日期：1982-06-01 00:00:00

  • The presence of the APP(swe) mutation in mice does not increase the vulnerability of cholinergic basal forebrain neurons to neuroinflammation.

    abstract：:Neuroinflammation, and elevated levels of inflammatory proteins, such as tumor necrosis factor-alpha, and the deposition of beta-amyloid may interact to contribute to the pathogenesis of Alzheimer's disease. We reproduced a component of the neuroinflammatory state within the basal forebrain cholinergic system, a regio...

    journal_title：Neuroscience

    pub_type： 杂志文章

    doi：10.1016/j.neuroscience.2004.01.050

    authors： Wenk GL,McGann-Gramling K,Hauss-Wegrzyniak B

    更新日期：2004-01-01 00:00:00

  • Stress and the developing adolescent brain.

    abstract：:Adolescence is a time of continued brain maturation, particularly in limbic and cortical regions, which undoubtedly plays a role in the physiological and emotional changes coincident with adolescence. An emerging line of research has indicated that stressors experienced during this crucial developmental stage may affe...

    journal_title：Neuroscience

    pub_type： 杂志文章,评审

    doi：10.1016/j.neuroscience.2012.10.048

    authors： Eiland L,Romeo RD

    更新日期：2013-09-26 00:00:00

  • Latent inhibition of conditioned taste aversion is not disrupted, but can be enhanced, by selective nucleus accumbens shell lesions in rats.

    abstract：:Latent inhibition is a form of negative priming in which repeated non-reinforced pre-exposures to a stimulus retard subsequent learning about the predictive significance of that stimulus. The nucleus accumbens shell and the anatomical projection it receives from the hippocampal formation have been attributed a pivotal...

    journal_title：Neuroscience

    pub_type： 杂志文章

    doi：10.1016/j.neuroscience.2005.10.032

    authors： Pothuizen HH,Jongen-Rêlo AL,Feldon J,Yee BK

    更新日期：2006-01-01 00:00:00

  • Bradykinin-induced release of calcitonin gene-related peptide from capsaicin-sensitive nerves in guinea-pig atria: mechanism of action and calcium requirements.

    abstract：:The mechanism of neuropeptide secretion induced by bradykinin from capsaicin-sensitive afferents was studied in guinea-pig atria. Both the inotropic response induced by bradykinin (0.1 microM) in the electrically driven isolated guinea-pig left atria and the bradykinin (10 microM)-induced release of calcitonin gene-re...

    journal_title：Neuroscience

    pub_type： 杂志文章

    doi：10.1016/0306-4522(90)90062-9

    authors： Geppetti P,Tramontana M,Santicioli P,Del Bianco E,Giuliani S,Maggi CA

    更新日期：1990-01-01 00:00:00

  • A possible role of tryptase in angiogenesis in the brain of mdx mouse, a model of Duchenne muscular dystrophy.

    abstract：:Duchenne muscular dystrophy (DMD) is characterized by muscle degeneration and affects the CNS. Dystrophin is absent in muscle and CNS of both DMD patients and mdx mouse, a model of DMD. While the involvement of vascular compartment in DMD was poorly investigated, some studies suggested a role for mast cells (MC). Tryp...

    journal_title：Neuroscience

    pub_type： 杂志文章

    doi：10.1016/j.neuroscience.2003.11.006

    authors： Nico B,Marzullo A,Corsi P,Vacca A,Roncali L,Ribatti D

    更新日期：2004-01-01 00:00:00

  • Electrical activity of neurons in the ventrolateral septum and bed nuclei of the stria terminalis in suckled rats: statistical analysis gives evidence for sensitivity to oxytocin and for relation to the milk-ejection reflex.

    abstract：:Our previous results obtained by lesioning or stimulating the ventrolateral part of the lateral septum and the bed nuclei of the stria terminalis suggested that this area is involved in the control of milk ejection pattern in rats. The present study was undertaken with the aim of testing ventrolateral part of the late...

    journal_title：Neuroscience

    pub_type： 杂志文章

    doi：10.1016/0306-4522(93)90258-h

    authors： Lambert RC,Moos FC,Ingram CD,Wakerley JB,Kremarik P,Guerné Y,Richard P

    更新日期：1993-05-01 00:00:00

  • Animal Use in Neurobiological Research.

    abstract：:The fact that neurobiological research is reliant upon laboratory-reared rodents is well known. The following paper discusses this topic broadly, but also aims to highlight other species used in the study of the nervous system and the evolution of animal species usage from the end of World War II through recent invest...

    journal_title：Neuroscience

    pub_type： 杂志文章,评审

    doi：10.1016/j.neuroscience.2020.02.049

    authors： Żakowski W

    更新日期：2020-05-01 00:00:00

  • Glycosaminoglycans boost insulin-like growth factor-I-promoted neuroprotection: blockade of motor neuron death in the wobbler mouse.

    abstract：:Wobbler mice display forelimb weakness, altered paw positioning, reduced running speed, muscle atrophy and motor neuron loss; co-treatment with glycosaminoglycans and insulin-like growth factor-I counteracts the progression of the disease. Reportedly, treatment with glycosaminoglycans or insulin-like growth factor-I s...

    journal_title：Neuroscience

    pub_type： 杂志文章

    doi：10.1016/s0306-4522(99)00095-0

    authors： Vergani L,Losa M,Lesma E,Di Giulio AM,Torsello A,Müller EE,Gorio A

    更新日期：1999-01-01 00:00:00

  • Maintenance of sympathetic tone by a nickel chloride-sensitive mechanism in the rostral ventrolateral medulla of the adult rat.

    abstract：:In urethane-anaesthetised artificially ventilated Sprague-Dawley rats, bilateral microinjection of the divalent cation nickel chloride (Ni(2+); 50 mM, 50 nl) into the rostral ventrolateral medulla elicited a dramatic inhibition of splanchnic sympathetic nerve activity (-44+/-6%) and a marked depressor response (-35+/-...

    journal_title：Neuroscience

    pub_type： 杂志文章

    doi：10.1016/s0306-4522(02)00705-4

    authors： Miyawaki T,Goodchild AK,Pilowsky PM

    更新日期：2003-01-01 00:00:00

  • Hypothermic preconditioning reduces Purkinje cell death possibly by preventing the over-expression of inducible nitric oxide synthase in rat cerebellar slices after an in vitro simulated ischemia.

    abstract：:We showed that hypothermic preconditioning (HPC) increased survival of Purkinje neurons in rat cerebellar slices after oxygen-glucose deprivation (OGD). HPC also reduced the OGD-increased expression of high mobility group I (Y) proteins, a transcription factor that can enhance inducible nitric oxide synthase (iNOS) ex...

    journal_title：Neuroscience

    pub_type： 杂志文章

    doi：10.1016/j.neuroscience.2006.06.053

    authors： Yuan HB,Huang Y,Zheng S,Zuo Z

    更新日期：2006-10-13 00:00:00

  • Genetic background regulates semaphorin gene expression and epileptogenesis in mouse brain after kainic acid status epilepticus.

    abstract：:The host response to neural injury, which can include axonal sprouting and synaptic reorganization is likely to be under tight genetic regulatory control at the level of the genome and may be implicated in epileptogenesis. Despite its importance, however, the molecular basis of synaptic reorganization is unclear. We h...

    journal_title：Neuroscience

    pub_type： 杂志文章

    doi：10.1016/j.neuroscience.2004.09.064

    authors： Yang J,Houk B,Shah J,Hauser KF,Luo Y,Smith G,Schauwecker E,Barnes GN

    更新日期：2005-01-01 00:00:00

  • Neuronal loss in functional zones of the cerebellum of chronic alcoholics with and without Wernicke's encephalopathy.

    abstract：:This study examines the effect of chronic alcohol consumption on the human cerebellum using operational criteria for case selection [Caine D. et al. (1997) J. Neurol. Neurosurg. Psychiat. 62, 51-60] and unbiased stereological techniques. We describe, for the first time, structural changes in different functional zones...

    journal_title：Neuroscience

    pub_type： 杂志文章

    doi：10.1016/s0306-4522(98)90664-9

    authors： Baker KG,Harding AJ,Halliday GM,Kril JJ,Harper CG

    更新日期：1999-01-01 00:00:00

  • What does autonomic arousal tell us about locomotor learning?

    abstract：:Walking onto a stationary sled previously experienced as moving induces locomotor aftereffects (LAE, or "broken escalator phenomenon"). This particular form of aftereffect can develop after a single adaptation trial and occurs despite subjects being fully aware that the sled will not move. Here, we investigate whether...

    journal_title：Neuroscience

    pub_type： 杂志文章,随机对照试验

    doi：10.1016/j.neuroscience.2010.06.079

    authors： Green DA,Bunday KL,Bowen J,Carter T,Bronstein AM

    更新日期：2010-09-29 00:00:00

  • The purinergic P2 receptor antagonist pyridoxalphosphate-6-azophenyl-2'4'-disulphonic acid prevents both the acute locomotor effects of amphetamine and the behavioural sensitization caused by repeated amphetamine injections in rats.

    abstract：:Repeated administration of amphetamine-like psychostimulants produce a progressive and long-lasting hypersensitivity to their behavioural effects known as behavioural sensitization. Previous studies have shown that administration of the purinergic P2 receptor agonist 2-methylthio ATP into the nucleus accumbens of rats...

    journal_title：Neuroscience

    pub_type： 杂志文章

    doi：10.1016/s0306-4522(00)00555-8

    authors： Kittner H,Krügel U,Illes P

    更新日期：2001-01-01 00:00:00

  • Neurochemical and anatomical identification of fast- and slow-firing neurones in the rat dorsal raphe nucleus using juxtacellular labelling methods in vivo.

    abstract：:GABA neurones in the dorsal raphe nucleus (DRN) influence ascending 5-hydroxytryptamine (5-HT) neurones but are not physiologically or anatomically characterised. Here, in vivo juxtacellular labelling methods in urethane-anaesthetised rats were used to establish the neurochemical and morphological identity of a fast-f...

    journal_title：Neuroscience

    pub_type： 杂志文章

    doi：10.1016/s0306-4522(03)00518-9

    authors： Allers KA,Sharp T

    更新日期：2003-01-01 00:00:00

  • Ascending projections from the solitary tract nucleus to the hypothalamus. A Phaseolus vulgaris lectin tracing study in the rat.

    abstract：:The course of the ascending pathways originating from the anterior gustatory and posterior visceral sensory part of the solitary tract nucleus and the topographic organization of the projections to the hypothalamus in the rat were studied with anterogradely transported Phaseolus vulgaris lectin. In general, the poster...

    journal_title：Neuroscience

    pub_type： 杂志文章

    doi：10.1016/0306-4522(89)90441-7

    authors： Ter Horst GJ,de Boer P,Luiten PG,van Willigen JD

    更新日期：1989-01-01 00:00:00

  • Stress Adaptation Upregulates Oxytocin within Hypothalamo-Vagal Neurocircuits.

    abstract：:Stress plays a pivotal role in the development and/or exacerbation of functional gastrointestinal (GI) disorders. The paraventricular nucleus of the hypothalamus (PVN) contains neurons that are part of the hypothalamic-pituitary-adrenal axis as well as preautonomic neurons innervating, among other areas, gastric-proje...

    journal_title：Neuroscience

    pub_type： 杂志文章

    doi：10.1016/j.neuroscience.2018.08.021

    authors： Jiang Y,Coleman FH,Kopenhaver Doheny K,Travagli RA

    更新日期：2018-10-15 00:00:00

  • Regulatory factor X1 depresses ApoE-dependent Aβ uptake by miRNA-124 in microglial response to oxidative stress.

    abstract：:Decreased proteolytic clearance of soluble amyloid β (Aβ) in microglia affects Aβ accumulation on Alzheimer's disease progression. However, the potential molecular mechanism by which microglial Aβ uptake is regulated remains unclear. In this study, we identified a microRNA, miR-124, that was down-regulated in aging wi...

    journal_title：Neuroscience

    pub_type： 杂志文章

    doi：10.1016/j.neuroscience.2016.12.017

    authors： Feng CZ,Yin JB,Yang JJ,Cao L

    更新日期：2017-03-06 00:00:00

  • Icariside II, a novel phosphodiesterase-5 inhibitor, attenuates streptozotocin-induced cognitive deficits in rats.

    abstract：:Beta-amyloid (Aβ) deposition and neuroinflammation are involved in Alzheimer's disease (AD)-type neurodegeneration with cognitive deficits. Phosphodiesterase-5 (PDE5) inhibitors have recently been studied as a potential target for cognitive enhancement by reducing inflammatory responses and Aβ levels. The present stud...

    journal_title：Neuroscience

    pub_type： 杂志文章

    doi：10.1016/j.neuroscience.2016.04.022

    authors： Yin C,Deng Y,Gao J,Li X,Liu Y,Gong Q

    更新日期：2016-07-22 00:00:00