Abstract:
:Apoptin, a small protein derived from chicken anemia virus, possesses the capacity to specifically kill tumor cells while leaving normal cells intact. Previous studies have indicated that the subcellular localization of apoptin appears to be crucial for this tumor-selective activity. Apoptin resides in the cytoplasm of normal cells; however, in cancer cells it translocates into the nucleus. In the present study, purified prokaryotic native His-apoptin served as a bait for capturing apoptin-associated proteins in both a hepatoma carcinoma cell line (HepG2) and a human fetal liver cell line (L-02). The captured proteins obtained from a pull-down assay were separated by two-dimensional gel electrophoresis. Mass spectrometry was employed to detect the effect of HSPA9 overexpression (one of the interacting proteins with apoptin in vitro) and downregulation of HSPA9 on HepG2 cells. The data revealed that HSPA9 overexpression resulted in partial distribution of apoptin in the cytoplasm. Notably, HSPA9 overexpression markedly decreased the apoptosis rate of HepG2 cells from 41.2 to 31.7%, while the downregulation of HSPA9 using small interfering RNA significantly enhanced the apoptosis of HepG2 cells. Our results suggest new insights into the localization mechanism of apoptin which is tightly associated with HSPA9 overexpression and its crucial role in cellular apoptosis both in a tumor cell line (HepG2) and a normal cell line (L-02). These findings shed new light on the elucidation of the underlying mechanism of anticancer action of apoptin.
journal_name
Oncol Repjournal_title
Oncology reportsauthors
Peng C,Yang P,Cui Y,He M,Liang L,Di Ydoi
10.3892/or.2013.2399subject
Has Abstractpub_date
2013-06-01 00:00:00pages
2431-7issue
6eissn
1021-335Xissn
1791-2431journal_volume
29pub_type
杂志文章相关文献
ONCOLOGY REPORTS文献大全abstract::Aberrant expression of microRNAs has been identified as regulators of biological processes of hepatocellular carcinoma (HCC) by negatively regulating protein-coding mRNAs. Several studies have demonstrated that miR-638 expression was dysregulated in various human cancers. However, the clinical significance and underly...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2016.5273
更新日期:2017-01-01 00:00:00
abstract::Lung cancer is the most frequent and deadliest cancer in the world, especially in China. However, the molecular mechanisms involved in lung cancer remain unclear. Occludin (OCLN), one of the first identified tight junction proteins, has been revealed to be a necessary integral protein for tight junction structure and ...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2018.6408
更新日期:2018-07-01 00:00:00
abstract::S-1 is an oral antitumor agent that contains tegafur, which is converted to fluorouracil (5-FU) in the human body. Cytochrome P450 2A6 (CYP2A6) is the principal enzyme responsible for bioconversion of tegafur to 5-FU. A number of CYP2A6 polymorphisms have been associated with variat...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2012.1678
更新日期:2012-05-01 00:00:00
abstract::The mammalian target of rapamycin (mTOR) is a key kinase acting downstream of growth factor receptor PI3K and AKT signaling, leading to processes resulting in increased cell size and proliferation through translation control. Rapamycin, a specific inhibitor of mTOR, results predomin...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2012.1855
更新日期:2012-09-01 00:00:00
abstract::Notch signaling plays a critical role in embryonic vascular development and tumor angiogenesis. The present study was conducted to investigate the prognostic role of the angiogenesis-related Notch ligand and the receptor in acute myeloid leukemia (AML) and assess whether their expre...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2012.1943
更新日期:2012-10-01 00:00:00
abstract::A-kinase can inhibit RhoA activation through phosphorylating ser188 of RhoA. AKAP is a novel protein that can target PKA to different subcellular compartment. Evidence has been presented that PKA anchorage by AKAP is important for the kinase to exert its function. This study analyzed the role of PKA anchorage in PKA-i...
journal_title:Oncology reports
pub_type: 杂志文章
doi:
更新日期:2006-10-01 00:00:00
abstract::This study was designed to investigate the effect of pseudo-G-Rh2, a novel metabolite of ginsenoside Rh2, on the apoptosis of SGC-7901 human gastric cancer cells. Pseudo-G-Rh2 demonstrated antitumor activity and significantly inhibited the proliferation of SGC-7901 cells in a concen...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2011.1442
更新日期:2011-12-01 00:00:00
abstract::Accumulation of an excess amount of reactive oxygen species (ROS) can cause hepatotoxicity that may result in liver damage. Therefore, development of anti-oxidative agents is needed for reducing liver toxicity. This study investigated the anti-oxidative and hepatoprotective activity of lithospermic acid, a plant-deriv...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2015.4068
更新日期:2015-08-01 00:00:00
abstract::Epigenetic anticancer drugs such as histone deacetylase (HDAC) inhibitors have been combined with existing anticancer drugs for synergistic or additive effects. In the present study, we found that a very low concentration of depsipeptide, an HDAC inhibitor, potentiated the antitumor activity of 5-fluorouracil (5-FU) i...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2016.5008
更新日期:2016-10-01 00:00:00
abstract::Casticin, a polymethoxyflavone, has been reported to exert anticancer activities. The objectives of this study were to examine the molecular mechanisms by which casticin induces the growth inhibition and cell cycle arrest in human hepatocellular carcinoma (HCC) cells. The HCC cell l...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2012.2076
更新日期:2013-01-01 00:00:00
abstract::Long non‑coding RNAs (lncRNAs) can act as carcinogenic or cancer suppressive factors during the pathogenesis, invasion and metastasis of non‑small cell lung cancer (NSCLC). The current study explored the role of long intergenic non‑protein coding RNA 00887 (LINC00887) and competing endogenous RNAs (ceRNAs). It was rev...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2019.7228
更新日期:2019-09-01 00:00:00
abstract::Human glioblastoma is a malignant and aggressive primary human brain solid tumor characterized by severe hypoxia. Hypoxia can induce autophagy, which may result in chemoresistance and malignant progression of cancer cells. MicroRNAs (miRNAs) have been reported to modulate hypoxia‑induced autophagy in various types of ...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2018.6929
更新日期:2019-03-01 00:00:00
abstract::Tripartite motif containing 28 (TRIM28) is a universal corepressor for Kruppel‑associated box zinc finger proteins. In our previous study, it was shown that expression of TRIM28 is upregulated in non‑small cell lung cancer (NSCLC) cell lines and tissues. Here, we demonstrated that the stable silencing of TRIM28 expres...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2017.5638
更新日期:2017-06-01 00:00:00
abstract::Burkitt's lymphoma (BL) is a fast growing cancer of the human lymphatic system, and an extremely invasive B-cell non-Hodgkin's lymphoma. We explored the mechanism of apoptosis in Raji cells associated with the post-transcriptional regulation factors. To confirm that the predicted microRNA-520a (miR-520a) is matched wi...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2016.4975
更新日期:2016-09-01 00:00:00
abstract::The antitumor effect of the human umbilical vein endothelial cell (HUVEC) vaccine has been well documented; however, its anti‑angiogenic effects on human esophageal squamous cell carcinoma (ESCC) have yet to be studied. In the present study, a 'humanized' mouse model was established by transplanting NOD/SCID mice with...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2018.6653
更新日期:2018-11-01 00:00:00
abstract::Sunitinib is a novel, multi-targeted receptor tyrosine kinase inhibitor, which has demonstrated evidence of improved survival when compared to interferon (IFN)-alpha in patients with metastatic renal cell carcinoma (RCC). Recently published National Institute for Health and Clinical Excellence guidance recommends suni...
journal_title:Oncology reports
pub_type: 临床试验,杂志文章
doi:10.3892/or_00000886
更新日期:2010-08-01 00:00:00
abstract::Subsequently to the publication of this paper, the authors have realized that the affilitation address omitted the name of the university at which this research was conducted (Capital Medical University). Therefore, the author affiliations for this paper should have appeared as follows (changes are highlighted in bold...
journal_title:Oncology reports
pub_type: 杂志文章,已发布勘误
doi:10.3892/or.2019.7385
更新日期:2019-12-01 00:00:00
abstract::The ability to detect K-ras oncogene may provide additional information for the management of patients with non-small cell lung cancer (NSCLC). In the present study, we detected the K-ras oncogene in 76 patients with NSCLC by two methods: direct sequencing of K-ras in tumor tissues and membrane array detection of the ...
journal_title:Oncology reports
pub_type: 杂志文章
doi:
更新日期:2007-07-01 00:00:00
abstract::Cell cycle-related molecules play crucial roles in maintaining genomic stability, and can also serve as biomarkers of cell cycle phase distribution at the same time. In this study, we used multiparameter analysis of various biomarkers to investigate their utility for the evaluation ...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2012.1894
更新日期:2012-09-01 00:00:00
abstract::Annexin A2 (ANXA2) is a well-known calcium-dependent phospholipid binding protein widely distributed in the nucleus, cytoplasm and extracellular surface of various eukaryotic cells. It has been recognized as a pleiotropic protein affecting a wide range of molecular and cellular processes. Dysregulation and abnormal ex...
journal_title:Oncology reports
pub_type: 杂志文章,评审
doi:10.3892/or.2015.3837
更新日期:2015-05-01 00:00:00
abstract::HIF-1 is a heterodimer consisting of the HIF-1alpha and HIF-1beta subunits, and HIF-1alpha is the unique oxygen regulated subunit that determines HIF-1 activity. HIF-1alpha upgrades many gene products which include the glucose transporter protein 1 (Glut-1). Immunohistochemical studies using a monoclonal antibody spec...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.14.4.909
更新日期:2005-10-01 00:00:00
abstract::The global rising incidence of hepatocellular carcinoma (HCC), which parallels the increase of hepatitis C virus (HCV) prevalence, has sparked a renewed interest in discovering additional HCC serum markers. In this study, we investigated the clinical use of serum E-cadherin, ICAM, MMP-2, VEGF, OPN and β-catenin as pot...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2011.1355
更新日期:2011-10-01 00:00:00
abstract::All-trans retinoic acid (ATRA) is a potent chemopreventive and therapeutic agent and exerts its effects by inducing growth arrest. In the present study, we demonstrated that ATRA activated the expression of p53 via Axin and induced cell cycle arrest at the G1/S phase and apoptosis of glioma cells. Briefly, C6 cells we...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2013.2391
更新日期:2013-06-01 00:00:00
abstract::Mutations in the epidermal growth factor receptor (EGFR) make lung adenocarcinoma cells sensitive to EGFR tyrosine kinase inhibitors (TKIs). Long-term cancer therapy may cause the occurrence of acquired resistance to EGFR TKIs. Long non-coding RNAs (lncRNAs) play important roles in tumor formation, tumor metastasis an...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2016.4758
更新日期:2016-06-01 00:00:00
abstract::The actual predictive value of oestrogen receptor (ER) beta for treatment decisions in breast cancer is still unclear. Retrospective studies using preoperative systemic therapy (PST) revealed that chemotherapy but also endocrine therapy can lead to alterations in expression levels of ERalpha and progesterone receptor ...
journal_title:Oncology reports
pub_type: 杂志文章
doi:
更新日期:2010-09-01 00:00:00
abstract::Following the publication of the above paper, an interested reader drew to our attention apparent anomalies associated with Figs. 2, 3 and 4; essentially, these three figures contained panels exhibiting overlapping data, such that data purportedly relating to different experiments were apparently drawn from the same o...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2020.7868
更新日期:2020-11-25 00:00:00
abstract::The aim of the present study was to research the apoptosis of human hepatocellular carcinoma cell line HepG 2 induced by pitavastatin. HepG 2 cells were treated with increasing doses of pitavastatin or with mevalonic acid for 48 h. The proliferation of cells was detected with WST-8. The morphology of the nucleus was o...
journal_title:Oncology reports
pub_type: 杂志文章
doi:
更新日期:2007-08-01 00:00:00
abstract::Cancer represents a significant challenge for humankind, as early diagnosis and treatment are difficult to achieve. To systemically investigate the effect of sex, body mass index (BMI) and age on cancer incidence and prognosis, the data from 14,504 cases of cancer were downloaded from The Cancer Genome Atlas (TCGA). B...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2018.6529
更新日期:2018-09-01 00:00:00
abstract::Lung cancer is still the leading cause of cancer-related deaths worldwide. Identifying new oncogenic drivers and developing efficient inhibitors through molecular targeting approaches are crucial for improving therapies. The aim of this study was to investigate whether targeting fibroblast growth factor receptor 1 (FG...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2013.2386
更新日期:2013-06-01 00:00:00
abstract::Emerging evidence suggests that cancer cells present profound epigenetic alterations in addition to featuring classic genetic mutations. Valproic acid (VPA), a histone deacetylase inhibitor, can potently inhibit tumor growth and induce differentiation. However, the effect and underlying mechanism of VPA on head and ne...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2015.4145
更新日期:2015-10-01 00:00:00