Abstract:
:Tripartite motif containing 28 (TRIM28) is a universal corepressor for Kruppel‑associated box zinc finger proteins. In our previous study, it was shown that expression of TRIM28 is upregulated in non‑small cell lung cancer (NSCLC) cell lines and tissues. Here, we demonstrated that the stable silencing of TRIM28 expression by a specific siRNA lentivirus vector increased the sensitivity of NSCLC cells to chemotherapeutic agent etoposide. Combination of TRIM28 siRNA and etoposide significantly inhibited the growth and proliferation of lung adenocarcinoma PAa cells and exerted obvious antitumor effects in nude mice. Using FCM and terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick-end labeling (TUNEL) assay, we found that TRIM28 siRNA in combination with etoposide increased apoptosis in vitro and in vivo which was induced by E2F1 activity, since the expression of E2F1 and its target genes was significantly increased in the cotreatment group. Cell proliferation and apoptosis were almost completely abolished in the PAa cells cotreated with TRIM28 siRNA and etoposide following knockdown of E2F1. The results of our study demonstrated that the combination of TRIM28 siRNA and etoposide may be effective against NSCLC and has the potential of being a new therapeutic tool for future treatment.
journal_name
Oncol Repjournal_title
Oncology reportsauthors
Liu L,Xiao L,Liang X,Chen L,Cheng L,Zhang L,Wu X,Xu Q,Ma Cdoi
10.3892/or.2017.5638subject
Has Abstractpub_date
2017-06-01 00:00:00pages
3597-3605issue
6eissn
1021-335Xissn
1791-2431journal_volume
37pub_type
杂志文章相关文献
ONCOLOGY REPORTS文献大全abstract::Krüppel-like factor 4 (KLF4) is a zinc finger transcription factor, which was confirmed as a tumor suppressor gene in colorectal cancers. KLF4 inhibits colorectal cancer cells proliferation through upregulating p21WAF1/Cip1 and downregulating cyclin D1. We firstly reported that N-Myc downstream regulated gene 2 (NDRG2...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2017.5736
更新日期:2017-08-01 00:00:00
abstract::Cancer represents a significant challenge for humankind, as early diagnosis and treatment are difficult to achieve. To systemically investigate the effect of sex, body mass index (BMI) and age on cancer incidence and prognosis, the data from 14,504 cases of cancer were downloaded from The Cancer Genome Atlas (TCGA). B...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2018.6529
更新日期:2018-09-01 00:00:00
abstract::MicroRNAs (miRNAs or miRs) contribute to the development of various malignant neoplasms, including glioblastoma multiforme (GBM). The present study aimed to explore the pathogenesis of GBM and to identify latent therapeutic agents for patients with GBM, based on an in silico analysis. Gene chips that provide miRNA exp...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2019.7022
更新日期:2019-04-01 00:00:00
abstract::Colorectal cancer (CRC) is the most commonly diagnosed tumor worldwide. However, the molecular mechanisms and biological processes of CRC remain unknown. The present study used reverse transcription-quantitative polymerase chain reaction to determine the expression levels of microRNA (miR)-296 in CRC cell lines and ti...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2018.6806
更新日期:2019-01-01 00:00:00
abstract::Pien Tze Huang (PZH) is a well-known traditional Chinese formula that was first prescribed by a royal physician in the Ming Dynasty. PZH has been used to treat various types of cancers including osteosarcoma. Previous studies have shown that PZH may effectively inhibit osteosarcoma cell growth in vivo and in vitro via...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2013.2916
更新日期:2014-02-01 00:00:00
abstract::A-kinase can inhibit RhoA activation through phosphorylating ser188 of RhoA. AKAP is a novel protein that can target PKA to different subcellular compartment. Evidence has been presented that PKA anchorage by AKAP is important for the kinase to exert its function. This study analyzed the role of PKA anchorage in PKA-i...
journal_title:Oncology reports
pub_type: 杂志文章
doi:
更新日期:2006-10-01 00:00:00
abstract::MicroRNAs (miRNAs) are small non-coding RNAs of 20-25 nucleotides in length that are capable of modulating gene expression post-transcriptionally. The potential roles of miRNAs in the tumorigenesis of glioblastoma (GBM) have been under intensive studies in the past few years. In the present study, we found a positive ...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2014.3055
更新日期:2014-05-01 00:00:00
abstract::In recent years an extensive series of synthetic combretastatin A-4 (CA-4)-azetidinone (β-lactam) hybrids were designed and synthesised with a view to improve the stability, therapeutic efficacy and aqueous solubility of CA-4. Lead compounds containing a 3,4,5-trimethoxy aromatic ring at position 1 and a variety of su...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2013.2379
更新日期:2013-06-01 00:00:00
abstract::Esophageal squamous cell carcinoma (ESCC) is the predominant subtype of human esophageal cancer in East Asia. In the present study, we explored the tumor suppressive function of microRNA-181d (miR-181d) in ESCC. Quantitative RT-PCR was used to assess gene levels of miR‑181d in both ESCC cell lines and clinical samples...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2016.5028
更新日期:2016-10-01 00:00:00
abstract::Epidermal growth factor receptor (EGFR) is a receptor tyrosine kinase and mutations in this gene are major drivers of lung cancer development. EGFR tyrosine kinase inhibitors (TKIs) are standard first‑line therapies for patients with advanced non‑small cell lung cancer (NSCLC) with activating EGFR mutations, but are n...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2019.7140
更新日期:2019-07-01 00:00:00
abstract::To investigate the antitumor action of arsenic trioxide (As2O3) by intratumoral injection into solid tumors, tumor growth inhibition (TGI) and angiogenesis of heterotransplanted esophageal carcinoma in mice was carried out. The cultured human esophageal carcinoma cells were inoculated into both laterals of the abdomin...
journal_title:Oncology reports
pub_type: 杂志文章
doi:
更新日期:2003-11-01 00:00:00
abstract::Oncolytic viruses are genetically engineered viruses designed for the treatment of solid tumors, and are often coupled with the antitumor immunity of the host. The challenge of using oncolytic herpes simplex virus (oHSV) as an efficacious oncolytic agent is the potential host tissue damage caused by the production of ...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2018.6935
更新日期:2019-03-01 00:00:00
abstract::Members of the Eph family of receptor tyrosine kinase have been implicated in cell-cell communication and tissue integrity during embryogenesis. We have previously demonstrated cell type specific and hormone dependent EphB4 expression in the mouse mammary parenchyma suggesting involvement in the homeostasis of this or...
journal_title:Oncology reports
pub_type: 杂志文章
doi:
更新日期:2002-09-01 00:00:00
abstract::Epigenetic mechanisms of histone acetylation/deacetylation play an important role in the regulation of gene expression associated with the cell cycle and apoptosis. Recently, sodium butyrate, a histone deacetylase (HDAC) inhibitor, has been shown to exhibit anticancer effects via di...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2011.1585
更新日期:2012-04-01 00:00:00
abstract::The therapeutic potential of membrane complement regulatory protein (mCRP)-neutralizing antibodies is unsatisfactory, which perhaps lies in the complex role of mCRPs in tumor occurrence and development. As a member of the mCRPs, CD46 is a transmembrane protein with a cytoplasmic domain and is implicated more in the co...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2013.2877
更新日期:2014-02-01 00:00:00
abstract::High-density single nucleotide polymorphism (SNP) array is a recently introduced technology that genotypes more than 10,000 human SNPs on a single array. It has been shown that SNP arrays can be used to determine not only SNP genotype calls, but also DNA copy number (DCN) aberrations, which are common in solid tumors....
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.15.4.1057
更新日期:2006-01-01 00:00:00
abstract::WNT signaling pathway networks with Hedgehog, Notch and FGF signaling pathways during carcinogenesis and embryogenesis. FZD8 is up-regulated in HeLa S3 and A549 cells. Here we identified and characterized rat Fzd8 gene by using bioinformatics. Rat Fzd8 gene was identified within AC131883.2 genome sequence. Rat Fzd8 (6...
journal_title:Oncology reports
pub_type: 杂志文章
doi:
更新日期:2005-05-01 00:00:00
abstract::SET domain bifurcated 1 (SETDB1) is a histone H3 lysine 9 methyltransferase that is highly expressed in various tumor types, including breast cancer. However, how SETDB1 functions in breast cancer is unclear. In the present study, proliferation, migration and invasion assays were performed to explore the role of SETDB...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2018.6871
更新日期:2019-02-01 00:00:00
abstract::We previously found that oleanolic acid (OA), a naturally pentacyclic triterpenoid, enhances the radiosensitizing effect on tumor cells. However, it is unclear whether or not OA enhances the radiosensitivity of hypoxic cells. Therefore, the aim of the present study was to further observe the influence of OA on hypoxic...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2014.3064
更新日期:2014-05-01 00:00:00
abstract::A 69-year-old woman presented to her local clinic with vomiting and abdominal distension. Since a bowel obstruction by left colon cancer was suspected due to a marked dilation of the transverse colon, she was referred to our hospital. On admission, an enema disclosed a complete obstruction at the splenic flexure of th...
journal_title:Oncology reports
pub_type: 杂志文章
doi:
更新日期:2008-04-01 00:00:00
abstract::Apoptosis repressor with caspase recruitment domain (ARC), an inhibitor of apoptosis, is primarily expressed in terminally differentiated tissues. Recent studies have revealed that ARC is highly expressed in a variety of human cancer cell lines and epithelial-derived cancers, which suggests that ARC plays an important...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2013.2622
更新日期:2013-10-01 00:00:00
abstract::Eighteen primary human malignant mesotheliomas obtained from 18 patients were screened for point mutations and microdeletions/insertions in all exons of the tumour suppressor gene PTEN/MMAC1 by SSCP analysis. No mutation could be found. Our preliminary data indicate that disarrangements of PTEN/MMAC1 are at least not ...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.8.6.1375
更新日期:2001-11-01 00:00:00
abstract::The decision to repeat transcatheter arterial chemoembolization (TACE) for hepatocellular carcinoma (HCC) is based on correct evaluation of response to therapy. The purpose of this study was to investigate whether apparent diffusion coefficient (ADC), a quantitative parameter of diffusion-weighted imaging (DWI), can p...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or_00000914
更新日期:2010-09-01 00:00:00
abstract::Anti-cytotoxic T lymphocyte antigen-4 (CTLA-4) treatment is effective for the treatment of primary tumors, but not sufficient for the treatment of metastatic tumors, likely owing to the effects of the tumor microenvironment. In this study, we aimed to determine the therapeutic effects of combined treatment with a matr...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2016.4547
更新日期:2016-03-01 00:00:00
abstract::Bee venom (BV) therapy is a type of alternative medical treatment used to treat various diseases in oriental medicine. The mechanisms underlying the effects of BV remain poorly understood. In the present study, we evaluated the antiviral effect of BV on cervical carcinoma cell lines (CaSki, HeLa, C33A and TC-1). BV tr...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2015.3760
更新日期:2015-04-01 00:00:00
abstract::In the present study, we analyzed the influence of brefeldin A (BFA) and castanospermine (CAS) on the activity, stability and localization of P-glycoprotein (Pgp) and breast cancer resistance protein (BCRP) in various resistant cell lines. The impact of BFA and CAS on cell viability was assessed using the MTT test. We...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2016.4656
更新日期:2016-05-01 00:00:00
abstract::Mitochondrial transcription factor A (TFAM), a high-mobility group (HMG) protein, plays a central role in mitochondrial DNA (mtDNA) replication, transcription and inheritance. It has been shown that TFAM is associated with tumorigenesis. However, little is known regarding the posttranscriptional regulation of TFAM in ...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2013.2712
更新日期:2013-11-01 00:00:00
abstract::The impact of interferon-γ (IFN-γ) late preconditioning on myocardial ischemia-reperfusion injury during cardiopulmonary bypass (CPB) and the underlying mechanism were investigated. Using a porcine model of myocardial ischemia-reperfusion injury during CPB with a 60-min aorta cross-clamp, 20 pigs (15±0.5 kg) were trea...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2013.2707
更新日期:2013-11-01 00:00:00
abstract::Despite significant advances in micro-metastasis detection methods, little is known about the relationship between micro-metastasis and primary tumors. The purpose of this study was to assess the ability of expression of the breast cancer-related markers, HER-2/neu, COX-2, VEGF and PDGF-B, as a predictor for micro-met...
journal_title:Oncology reports
pub_type: 杂志文章
doi:
更新日期:2006-05-01 00:00:00
abstract::Gastric cancer (GC) is one of the most common causes of cancer‑related mortality worldwide. Despite remarkable progress in the diagnosis and treatment of GC, a large number of cases are diagnosed as advanced GC, and treatment failure occurs. Emerging evidence has shown that non‑coding RNAs (ncRNAs), especially microRN...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2020.7705
更新日期:2020-10-01 00:00:00