当前位置： SCI文献检索 > ONCOLOGY REPORTS期刊下所有文献 > Characteristic CYP2A6 genetic polymorphisms detected by TA cloning-based sequencing in Chinese digestive system cancer patients with S-1 based chemotherapy.

Characteristic CYP2A6 genetic polymorphisms detected by TA cloning-based sequencing in Chinese digestive system cancer patients with S-1 based chemotherapy.

Abstract：

:S-1 is an oral antitumor agent that contains tegafur, which is converted to fluorouracil (5-FU) in the human body. Cytochrome P450 2A6 (CYP2A6) is the principal enzyme responsible for bioconversion of tegafur to 5-FU. A number of CYP2A6 polymorphisms have been associated with variations in enzyme activity in several ethnic populations. The CYP2A6*4C allele leads to deletion of the entire CYP2A6 gene, and is the main finding in patients with reduced CYP2A6 enzymatic activity. Thus, the aim of our study was to evaluate the allele frequencies of CYP2A6 polymorphisms in a population with cancer of the digestive system. We developed a simple screening method, which combined TA cloning and direct-sequencing, to detect CYP2A6 genetic polymorphisms in Chinese patients with cancers of the digestive system. A total of 77 patients with various types of digestive system cancers were screened for CYP2A6 genetic polymorphisms. The allele frequencies of CYP2A6*1A, CYP2A6*1B and CYP2A6*4C in the 77 patients screened were 62, 42 and 13%, respectively. Frequencies of the homozygous genotypes for CYP2A6*1A and CYP2A6*4C were 27 and 12%, respectively. As expected, patients that were determined to be homozygous for CYP2A6*4C exhibited the characteristic chemotherapy efficacy and toxicity profiles. The TA cloning-based direct sequencing method facilitated allele frequency and genotyping determination for CYP2A6*1A, 1B and 4C of cancer patients. The findings indicated that the population carries a high frequency of the CYP2A6*4C homozygous genotype. Thus, the reduced efficacy of standard chemotherapy dosage in Chinese cancer patients may be explained by the lack of CYP2A6-mediated S-1 bioconversion to 5-FU.

journal_name

Oncol Rep

journal_title

Oncology reports

authors

Fang WJ,Mou HB,Jin DZ,Zheng YL,Zhao P,Mao CY,Peng L,Huang MZ,Xu N

doi

10.3892/or.2012.1678

subject

Has Abstract

pub_date

2012-05-01 00:00:00

pages

1606-10

issue

5

eissn

1021-335X

issn

1791-2431

journal_volume

27

pub_type

杂志文章

相关文献

ONCOLOGY REPORTS文献大全
  • Internal mammary sentinel node biopsy for breast cancer: is it practicable and relevant? (Review).

    abstract：:Sentinel lymph node (SLN) biopsy is a useful way of assessing axillary nodal status and obviates unnecessary axillary lymph node dissection for patients with node-negative breast cancer. However, SLN can also be located in the internal mammary lymph node (IMN) chain or elsewhere. The practicability and relevance of in...

    journal_title：Oncology reports

    pub_type： 杂志文章,评审

    doi：

    authors： Noguchi M

    更新日期：2002-05-01 00:00:00

  • Phase I study of S-1 and biweekly docetaxel combination chemotherapy for advanced and recurrent gastric cancer.

    abstract：:A phase I study of S-1 and biweekly docetaxel (DOC) combination therapy was conducted to determine the maximum tolerated dose (MTD) and pharmacokinetic parameters. Fourteen patients with advanced or recurrent gastric cancer were analyzed. The treatment consisted of S-1 [body surface area (BSA) <1.25 m2:80 mg/day, 1.25...

    journal_title：Oncology reports

    pub_type： 杂志文章

    doi：

    authors： Takahashi I,Emi Y,Kakeji Y,Tokunaga E,Ushiro S,Oki E,Watanabe M,Baba H,Maehara Y

    更新日期：2006-04-01 00:00:00

  • O6-methylguanine-DNA methyltransferase promoter hypermethylation in colorectal carcinogenesis.

    abstract：:Epigenetic alterations have been reported in colorectal neoplasia which can either complement or in some cases be predisposed to genetic alterations such as K-ras mutations. We examined the promoter methylation status of the CDKN2A and O6-methylguanine-DNA methyltransferase (MGMT) genes, after sodium bisulfite convers...

    journal_title：Oncology reports

    pub_type： 杂志文章

    doi：

    authors： Menigatti M,Pedroni M,Verrone AM,Borghi F,Scarselli A,Benatti P,Losi L,Di Gregorio C,Schär P,Marra G,Ponz de Leon M,Roncucci L

    更新日期：2007-06-01 00:00:00

  • Accuracy of criteria for predicting recurrence and metastasis in stage II and III gastric cancer patients with lymph node metastasis.

    abstract：:This study assessed the prediction of gastric cancer recurrence based on detection of occult neoplastic cells (ONCs) in lymph nodes, and by using criteria that were developed to identify patients with a high risk of recurrence/metastasis. The subjects were 60 patients of stage II or III gastric cancer with lymph node ...

    journal_title：Oncology reports

    pub_type： 杂志文章

    doi：

    authors： Mukai M,Sato S,Komatsu N,Kimura T,Ninomiya H,Kawada M,Nakasaki H,Ogoshi K,Makuuchi H

    更新日期：2004-07-01 00:00:00

  • Role of ERCC5 polymorphisms in non‑small cell lung cancer risk and responsiveness/toxicity to cisplatin‑based chemotherapy in the Chinese population.

    abstract：:Non‑small cell lung cancer (NSCLC) is the leading cause of cancer‑related deaths worldwide. Cisplatin‑based chemotherapy currently represents the main treatment option for patients with NSCLC. The aim of the present study was to evaluate effect of single nucleotide polymorphisms (SNPs) within the excision repair cross...

    journal_title：Oncology reports

    pub_type： 杂志文章

    doi：10.3892/or.2021.7935

    authors： Li M,Chen R,Ji B,Fan C,Wang G,Yue C,Jin G

    更新日期：2021-01-14 00:00:00

  • Curcumin and paclitaxel induce cell death in breast cancer cell lines.

    abstract：:Breast cancer is one of the major health issues confronting women; however, treatment with conventional chemotherapeutic drugs is limited. Currently, paclitaxel is used as a therapeutic clinical agent to treat breast cancer that exerts antitumor activity in numerous types of cancer cell. Curcumin (diferuloylmethane), ...

    journal_title：Oncology reports

    pub_type： 杂志文章

    doi：10.3892/or.2018.6603

    authors： Calaf GM,Ponce-Cusi R,Carrión F

    更新日期：2018-10-01 00:00:00

  • Thioridazine, an antipsychotic drug, elicits potent antitumor effects in gastric cancer.

    abstract：:Thioridazine, an antipsychotic drug, has been reported to induce apoptosis in various types of cancer cells, with specificity on targeting cancer stem cells (CSCs). However, whether it elicits anticancer effects in gastric cancer has never been reported. In the present study, we examined the ability of thioridazine to...

    journal_title：Oncology reports

    pub_type： 杂志文章

    doi：10.3892/or.2014.3068

    authors： Mu J,Xu H,Yang Y,Huang W,Xiao J,Li M,Tan Z,Ding Q,Zhang L,Lu J,Wu X,Liu Y

    更新日期：2014-05-01 00:00:00

  • Effect of wortmannin on the suppression of thermotolerance in tsAF8 cells.

    abstract：:Phosphatidylinositol 3 kinases (PI-3 kinases) play a crucial role in the inositol phospholipid signaling pathway. The effect of wortmannin, an inhibitor of PI-3 kinases, on the suppression of thermotolerance development was investigated in the temperature-sensitive cell line tsAF8. When wortmannin was added immediatel...

    journal_title：Oncology reports

    pub_type： 杂志文章

    doi：

    authors： Shibuya K,Kawasaki S,Kuroda M,Takemoto M,Asaumi J,Hiraki Y

    更新日期：2002-01-01 00:00:00

  • Lack of prognostic significance of nm23 expression in human primary breast cancer.

    abstract：:This retrospective study was designed to evaluate the prognostic value of immunohistochemically detected nm23-expression in invasive breast cancer. Cellular expression of nm23 was assessed in 325 primary breast cancer tissues by immunohistochemistry. The data were correlated to established functional factors of progno...

    journal_title：Oncology reports

    pub_type： 杂志文章

    doi：

    authors： Göhring UJ,Eustermann I,Becker M,Neuhaus W,Rein DT,Schöndorf T

    更新日期：2002-11-01 00:00:00

  • In vivo evidence for a significant role of folylpolyglutamate synthase in combined chemotherapy with oral fluoropyrimidine, UFT or S-1, and leucovorin.

    abstract：:Combined chemotherapy with 5-fluorouracil and leucovorin (LV) has been widely used for the treatment of patients with colorectal cancer. Given that LV effects are attributable to increased levels of reduced folate in cancer cells, we attempted here to show the in vivo role of folylpolyglutamate synthetase (FPGS), whic...

    journal_title：Oncology reports

    pub_type： 杂志文章

    doi：10.3892/or.2011.1206

    authors： Tsukioka S,Sakamoto E,Tsujimoto H,Nakagawa F,Saito H,Uchida J,Kiniwa M,Fukushima M

    更新日期：2011-05-01 00:00:00

  • L‑Deprenyl exerts cytotoxicity towards acute myeloid leukemia through inhibition of mitochondrial respiration.

    abstract：:The identification of large numbers of genetic mutations in immature myeloid cells has made it difficult to identify specific targets for acute myeloid leukemia (AML) therapy. Although current pharmacological targets for controlling cancer are focused on identifying genetic mutations, it is hard to develop the specifi...

    journal_title：Oncology reports

    pub_type： 杂志文章

    doi：10.3892/or.2018.6753

    authors： Ryu I,Ryu MJ,Han J,Kim SJ,Lee MJ,Ju X,Yoo BH,Lee YL,Jang Y,Song IC,Chung W,Oh E,Heo JY,Kweon GR

    更新日期：2018-12-01 00:00:00

  • Somatic VHL alteration and its impact on prognosis in patients with clear cell renal cell carcinoma.

    abstract：:Somatic inactivation of the von Hippel-Lindau (VHL) gene is the most frequent genetic event observed in clear cell renal cell carcinoma (CC-RCC). However, the prognostic relevance of somatic VHL alteration and its target, hypoxia inducible factor (HIF)-1alpha has not been defined. We investigated the genetic changes i...

    journal_title：Oncology reports

    pub_type： 杂志文章

    doi：

    authors： Kim JH,Jung CW,Cho YH,Lee J,Lee SH,Kim HY,Park J,Park JO,Kim K,Kim WS,Park YS,Im YH,Kang WK,Park K

    更新日期：2005-05-01 00:00:00

  • Dysregulation of apoptosis is a major mechanism in the lymph node involvement in colorectal carcinoma.

    abstract：:The purpose of this study was to define gene expression profile changes in colorectal tumors in order to identify target genes involved in neoplastic progression. cDNA microarray analysis was used to detect differences in gene expression profiles between colon tumor samples obtained from 20 patients in different tumor...

    journal_title：Oncology reports

    pub_type： 杂志文章

    doi：

    authors： Bandres E,Catalan V,Sola I,Honorato B,Cubedo E,Cordeu L,Andion E,Escalada A,Zarate R,Salgado E,Zabalegui N,García F,Garcia-Foncillas J

    更新日期：2004-08-01 00:00:00

  • Long non‑coding RNA RP1‑163G9.1 is downregulated in gastric adenocarcinoma and is associated with a poor prognosis.

    abstract：:The aim of the present study was to investigate the expression, function and underlying molecular mechanism of the long non‑coding (lnc) RNA RP1‑163G9.1 in patients with gastric adenocarcinoma (GA). The expression levels of lncRNA RP1‑163G9.1 were determined in 112 paired clinical GA tissues by reverse transcription‑q...

    journal_title：Oncology reports

    pub_type： 杂志文章

    doi：10.3892/or.2019.7127

    authors： Sun B,Dang Y,Zhang F,Li K,Ouyang X,Wang K,Huang Q

    更新日期：2019-06-01 00:00:00

  • Dexamethasone induces aberrant macrophage immune function and apoptosis.

    abstract：:Glucocorticoids (GCs) are known potent clinical drugs, however, their mode of action is still complex and debatable. Macrophages are the most important target of GCs and play a key role in tumor immunity in vivo, but their relationship is also controversial. In the present study, the lentivirus system was used to over...

    journal_title：Oncology reports

    pub_type： 杂志文章

    doi：10.3892/or.2019.7434

    authors： Ai F,Zhao G,Lv W,Liu B,Lin J

    更新日期：2020-02-01 00:00:00

  • Alteration of CD4 T cell subsets in metastatic lymph nodes of human gastric cancer.

    abstract：:Studies of tumor-infiltrating immune cells have revealed that immune escape plays an important role in tumor growth. The aim of the present study was to investigate the impact of metastasis affecting CD4+ T cell subsets in human clinical samples. Single-cell suspensions derived from tumor-draining lymph node (TDLN) an...

    journal_title：Oncology reports

    pub_type： 杂志文章

    doi：10.3892/or.2015.4064

    authors： Okita Y,Ohira M,Tanaka H,Tokumoto M,Go Y,Sakurai K,Toyokawa T,Kubo N,Muguruma K,Sawada T,Maeda K,Hirakawa K

    更新日期：2015-08-01 00:00:00

  • Fetal fibronectin: a new screening-marker for bladder cancer?

    abstract：:Early detection of transitional cell carcinoma (TCC) of the urinary bladder is essential for effective treatment. While several serum markers have been evaluated, none have been widely accepted for practical clinical use. Thus, urinary markers have been introduced and investigated to detect the evidence of bladder can...

    journal_title：Oncology reports

    pub_type： 杂志文章

    doi：10.3892/or.8.3.669

    authors： Wunderlich H,Reichelt O,Zermann DH,Schubert J,Berndt A,Kosmehl H

    更新日期：2001-05-01 00:00:00

  • An eight-long non-coding RNA signature as a candidate prognostic biomarker for lung cancer.

    abstract：:Cumulative evidence suggests that long non-coding RNAs (lncRNAs) may be good biomarkers in various types of tumors. In the present study, we mined lncRNA expression profiling in 739 lung cancer patients from Gene Expression Omnibus (GEO) datasets. A risk score model was constructed based on the expression data of thes...

    journal_title：Oncology reports

    pub_type： 杂志文章

    doi：10.3892/or.2016.4817

    authors： Tu Z,He D,Deng X,Xiong M,Huang X,Li X,Hao L,Ding Q,Zhang Q

    更新日期：2016-07-01 00:00:00

  • Hepatitis B virus X protein mutant upregulates CENP-A expression in hepatoma cells.

    abstract：:The carcinogenic role of hepatitis B virus x protein (HBx) in hepatocellular carcinoma (HCC) remains largely unknown. Centromere protein A (CENP-A) has been found to be frequently overexpressed in HCC. In the present study, we aimed to investigate the role of HBx in regulating CENP-...

    journal_title：Oncology reports

    pub_type： 杂志文章

    doi：10.3892/or.2011.1478

    authors： Liu L,Li Y,Zhang S,Yu D,Zhu M

    更新日期：2012-01-01 00:00:00

  • Death receptor-dependent and -independent pathways in anticancer drug-induced apoptosis of breast cancer cells.

    abstract：:Apoptosis is a crucial event for anticancer drug efficacy. The signal pathways activated by anticancer drugs are classified as death receptor (DR) -dependent or -independent. The mechanisms by which anticancer drugs induce apoptosis via DR-dependent pathways are not fully understood. In the present study, we assessed ...

    journal_title：Oncology reports

    pub_type： 杂志文章

    doi：

    authors： Kim R,Tanabe K,Emi M,Uchida Y,Toge T

    更新日期：2003-11-01 00:00:00

  • Human DNA topoisomerase II-alpha expression in laparoscopically treated renal cell carcinoma.

    abstract：:To investigate whether the expression of Topoisomerase II-alpha (TII-alpha) can serve as a prognostic factor in renal cell carcinoma (RCC), histological sections from 27 renal tumors were stained immunohistochemically for TII-alpha expression. The percentage of positive cells in the area of greatest staining was recor...

    journal_title：Oncology reports

    pub_type： 杂志文章

    doi：

    authors： Dekel Y,Frede T,Kugel V,Neumann G,Rassweiler J,Koren R

    更新日期：2005-07-01 00:00:00

  • Radiosensitization, after a combined treatment of survivin siRNA and irradiation, is correlated with the activation of caspases 3 and 7 in a wt-p53 sarcoma cell line, but not in a mt-p53 sarcoma cell line.

    abstract：:Survivin, a member of the inhibitor-of-apoptosis family is an essential protein for regular mitosis and is involved in an anti-apoptotic pathway. In some studies, an association between survivin expression and radiosensitivity has been described for tumor cells, but the relationship between p53 and survivin regarding ...

    journal_title：Oncology reports

    pub_type： 杂志文章

    doi：

    authors： Kappler M,Taubert H,Bartel F,Blümke K,Panian M,Schmidt H,Dunst J,Bache M

    更新日期：2005-01-01 00:00:00

  • Mina53 nuclear localization is an important indicator of prognosis in patients with colorectal cancer after adjuvant chemotherapy.

    abstract：:The aim of this study was to investigate the status of the c‑Myc‑related molecule Mina53 and the clinical impact of Mina53 nuclear localization in patients with stage II and III colorectal cancer (CRC). Patients (n=250) who underwent complete resection of CRC at our department were enrolled in this study, and tissue m...

    journal_title：Oncology reports

    pub_type： 杂志文章

    doi：10.3892/or.2018.6407

    authors： Fujino S,Kinugasa T,Sudo T,Mizobe T,Yoshida T,Yoshida N,Ohchi T,Tajiri K,Yuge K,Nagasu S,Katagiri M,Akagi Y

    更新日期：2018-07-01 00:00:00

  • Nras and Kras mutation in Japanese lung cancer patients: Genotyping analysis using LightCycler.

    abstract：:Activating mutations of Ras gene families have been found in a variety of human malignancies, including lung cancer, suggesting their dominant role in tumorigenesis. Many studies have showed that the Kras gene is activated by point mutations in approximately 15-20% of non-small cell lung cancers (NSCLCs), however, the...

    journal_title：Oncology reports

    pub_type： 杂志文章

    doi：

    authors： Sasaki H,Okuda K,Kawano O,Endo K,Yukiue H,Yokoyama T,Yano M,Fujii Y

    更新日期：2007-09-01 00:00:00

  • Calphostin C, a remarkable multimodal photodynamic killer of neoplastic cells by selective nuclear lamin B1 destruction and apoptogenesis (Review).

    abstract：:Perylenequinones that generate reactive oxygen species (ROS) when illuminated with visible light have been recommended as photodynamic chemotherapeutic agents. One of these is calphostin C (CalC), the action of the photo-activated derivative of which, CalCphiE, has been ascribed to its ability to selectively and irrev...

    journal_title：Oncology reports

    pub_type： 杂志文章,评审

    doi：10.3892/or_00000711

    authors： Chiarini A,Whitfield JF,Pacchiana R,Marconi M,Armato U,Dal Prà I

    更新日期：2010-04-01 00:00:00

  • MicroRNA-124 inhibits cell proliferation and migration by regulating SNAI2 in breast cancer.

    abstract：:MicroRNA (miRNA) is a type of endogenous non‑coding RNA implicated in various cellular processes. Studies have shown that miR-124 is involved in the malignant progression of cancer, but little is known concerning its potential role in breast cancer. Therefore, the purpose of this study was to conduct a functional anal...

    journal_title：Oncology reports

    pub_type： 杂志文章

    doi：10.3892/or.2016.5163

    authors： Du S,Li H,Sun X,Li D,Yang Y,Tao Z,Li Q,Liu K

    更新日期：2016-12-01 00:00:00

  • miR-365 targets ADAM10 and suppresses the cell growth and metastasis of hepatocellular carcinoma.

    abstract：:Expression of miR-365 has been reported to be downregulated in hepatocellular carcinoma (HCC). However, the biological function and underlying mechanism of miR-365 in HCC growth and metastasis remain unclear. The aim of the present study was to explore the role of miR-365 in HCC progression. We found that miR-365 expr...

    journal_title：Oncology reports

    pub_type： 杂志文章

    doi：10.3892/or.2017.5423

    authors： Liu Y,Zhang W,Liu S,Liu K,Ji B,Wang Y

    更新日期：2017-03-01 00:00:00

  • Skp2, p27kip1 and EGFR assessment in head and neck squamous cell carcinoma: prognostic implications.

    abstract：:EGFR, p27kip1 and Skp2, have been implicated in human cancer development. We have studied these molecules in a search for molecular markers that may have prognostic value in head and neck squamous cell carcinomas (HNSCC). Tissue samples of 62 patients were collected and immunohistochemical analysis was carried out for...

    journal_title：Oncology reports

    pub_type： 杂志文章

    doi：

    authors： Carracedo DG,Astudillo A,Rodrigo JP,Suarez C,Gonzalez MV

    更新日期：2008-09-01 00:00:00

  • Programmed cell death 2 protein induces gastric cancer cell growth arrest at the early S phase of the cell cycle and apoptosis in a p53-dependent manner.

    abstract：:Programmed cell death 2 (PDCD2) is a highly conserved nuclear protein, and aberrant PDCD2 expression alters cell apoptosis. The present study aimed to investigate PDCD2 expression in gastric cancer. Tissue specimens from 34 gastric cancer patients were collected for analysis of PDCD2 expression using immunohistochemis...

    journal_title：Oncology reports

    pub_type： 杂志文章

    doi：10.3892/or.2014.3551

    authors： Zhang J,Wei W,Jin HC,Ying RC,Zhu AK,Zhang FJ

    更新日期：2015-01-01 00:00:00

  • Differential expression of nuclear lamins in normal and cancerous prostate tissues.

    abstract：:The process of carcinogenesis is characterized by definite changes in the protein composition of the nuclear matrix. We have recently found that lamins form, in addition to the nuclear lamina, an intranuclear web of thin fibrils. This finding prompted us to address the question of whether changes in the expression of ...

    journal_title：Oncology reports

    pub_type： 杂志文章

    doi：

    authors： Coradeghini R,Barboro P,Rubagotti A,Boccardo F,Parodi S,Carmignani G,D'Arrigo C,Patrone E,Balbi C

    更新日期：2006-03-01 00:00:00