Thioridazine, an antipsychotic drug, elicits potent antitumor effects in gastric cancer.

Abstract:

:Thioridazine, an antipsychotic drug, has been reported to induce apoptosis in various types of cancer cells, with specificity on targeting cancer stem cells (CSCs). However, whether it elicits anticancer effects in gastric cancer has never been reported. In the present study, we examined the ability of thioridazine to induce cell death in the gastric cancer cell lines NCI-N87 and AGS, and detected its in vivo tumor inhibition capacity. Thioridazine elicited cytotoxic effects on NCI-N87 and AGS cells in a dose-dependent manner, and inhibited the colony formation abilitiy of the NCI-N87 and AGS cells. Thioridazine treatment induced nuclear fragmentation, increased the proportion of sub-G1 phase cells, and elevated the percentage of Annexin V-positive cells, suggesting the occurrence of apoptosis. Moreover, thioridazine induced gastric cancer cell apoptosis in a caspase-dependent manner, as shown by a decrease in the precursors of casapse-9, caspase-8 and caspase-3, and by the ability of the caspase inhibitor Z-VAD-FMK to reverse the cytotoxic effect of thioridazine. JC-1 staining further revealed that thioridazine induced gastric cancer cell apoptosis via the mitochondrial pathway. In addition, thioridazine pretreatment inhibited the growth of NCI-N87 cell-derived tumors. The present study demonstrated that the antipsychotic drug thioridazine possesses anti-gastric cancer ability through in vitro and in vivo experiments, suggesting thioridazine as a potential drug in gastric cancer therapy.

journal_name

Oncol Rep

journal_title

Oncology reports

authors

Mu J,Xu H,Yang Y,Huang W,Xiao J,Li M,Tan Z,Ding Q,Zhang L,Lu J,Wu X,Liu Y

doi

10.3892/or.2014.3068

subject

Has Abstract

pub_date

2014-05-01 00:00:00

pages

2107-14

issue

5

eissn

1021-335X

issn

1791-2431

journal_volume

31

pub_type

杂志文章
  • Long non‑coding RNA RP1‑163G9.1 is downregulated in gastric adenocarcinoma and is associated with a poor prognosis.

    abstract::The aim of the present study was to investigate the expression, function and underlying molecular mechanism of the long non‑coding (lnc) RNA RP1‑163G9.1 in patients with gastric adenocarcinoma (GA). The expression levels of lncRNA RP1‑163G9.1 were determined in 112 paired clinical GA tissues by reverse transcription‑q...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or.2019.7127

    authors: Sun B,Dang Y,Zhang F,Li K,Ouyang X,Wang K,Huang Q

    更新日期:2019-06-01 00:00:00

  • Immunolabelling of the inhibin/activin-βC subunit in normal and malignant human uterine cervical tissue and cervical cancer cell lines.

    abstract::Inhibins are dimeric glycoproteins, composed of an α-subunit and one of two possible β-subunits (βA or βB), with substantial roles in human reproduction and in endocrine-responsive tumours. Recently a novel β subunit named βC was described, although it is still unclear if normal or cancerous cervical epithelial cells ...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:

    authors: Blankenstein T,Jückstock J,Shabani N,Kunze S,Brüning A,Bergauer F,Mylonas I

    更新日期:2011-01-01 00:00:00

  • Epigenetic changes of tumor suppressor genes, P15, P16, VHL and P53 in oral cancer.

    abstract::We performed methylation specific PCR to explore the mechanism of inactivation of tumor suppressor genes P15, P16, P53 and VHL in 48 oral SCC. The frequencies of aberrant methylation on the promoter of the P15, the P16, the P53 and the VHL genes were 0.27 (13/48), 0.42 (20/48), 0.04 (2/48) and none, respectively. Alto...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:

    authors: Yeh KT,Chang JG,Lin TH,Wang YF,Tien N,Chang JY,Chen JC,Shih MC

    更新日期:2003-05-01 00:00:00

  • Protocadherin-10 acts as a tumor suppressor gene, and is frequently downregulated by promoter methylation in pancreatic cancer cells.

    abstract::Protocadherin-10 (PCDH10), a member of non-clustered protocadherin family which plays important roles in calcium-dependent cell-cell signal transduction and adhesion. PCDH10 functions as a tumor suppressor gene and its expression is downregulated by promoter methylation in many malignances. In the present study, we ex...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or.2016.4793

    authors: Qiu C,Bu X,Jiang Z

    更新日期:2016-07-01 00:00:00

  • In vivo and in situ imaging of head and neck squamous cell carcinoma using near-infrared fluorescent quantum dot probes conjugated with epidermal growth factor receptor monoclonal antibodies in mice.

    abstract::In this study, we applied near-infrared fluorescent quantum dots (NIRF-QDs) for non-invasive in vivo and in situ imaging of head and neck squamous cell carcinoma (HNSCC). The U14 squamous cancer cell line with high expression of epidermal growth factor receptor (EGFR) was implanted subcutaneously into the head and nec...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or.2012.1705

    authors: Yang K,Zhao C,Cao YA,Tang H,Bai YL,Huang H,Zhao CR,Chen R,Zhao D

    更新日期:2012-06-01 00:00:00

  • ERK/GSK3β signaling is involved in atractylenolide I-induced apoptosis and cell cycle arrest in melanoma cells.

    abstract::Novel agents need to be developed to overcome the limitations of the current melanoma therapeutics. Atractylenolide I (AT-I) is a sesquiterpene compound isolated from atractylodis macrocephalae rhizoma. Previous findings demonstrated that AT-I exhibited cytotoxic action in melanoma cells. However, the molecular mechan...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or.2015.4111

    authors: Ye Y,Chao XJ,Wu JF,Cheng BC,Su T,Fu XQ,Li T,Guo H,Tse AK,Kwan HY,Du J,Chou GX,Yu ZL

    更新日期:2015-09-01 00:00:00

  • Sequencing hormonal ablation and radiotherapy in prostate cancer: a molecular and therapeutic prespective (Review).

    abstract::Current therapeutic modalities for advanced prostate cancer are palliative in nature, with no effective treatment increasing survival in patients with metastatic prostate cancer. Apoptosis as a molecular process of genetically regulated cell death has a critical endpoint that coincides with the goal of successful trea...

    journal_title:Oncology reports

    pub_type: 杂志文章,评审

    doi:

    authors: Hill B,Kyprianou N

    更新日期:2002-11-01 00:00:00

  • Role of v-Raf and truncated form RAF1 in the induction of vascular endothelial growth factor and vascularization.

    abstract::In this work we studied the effect of v-raf and different form RAF on up regulation of VEGF gene expression and angiogenesis. In the experiments the truncated forms of RAF1 gene were transfected into the cells of the established human urothelial line HCV-29, otherwise non-angiogenic. The plasmids coding for mitochondr...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:

    authors: Malecki M,Seneta M,Miloszewska J,Trembacz H,Przybyszewska M,Janik P

    更新日期:2004-01-01 00:00:00

  • Expression and promoter methylation of the RASSF1A gene in sporadic breast cancers in Chinese women.

    abstract::The novel tumor suppressor RASSF1A is frequently inactivated during human tumorigenesis by promoter methylation. In this study, we detected the RASSF1A promoter methylation by methylated-specific PCR and investigated RASSF1A gene expression by semi-quantitative RT-PCR and immunohistochemical staining in 36 cases of br...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:

    authors: Li Y,Wei Q,Cao F,Cao X

    更新日期:2008-05-01 00:00:00

  • Leucine zipper structure of TSC-22 (TGF-beta stimulated clone-22) markedly inhibits the anchorage-independent growth of salivary gland cancer cells.

    abstract::Several investigators have demonstrated that TGF-beta stimulated clone-22 (TSC-22) regulates cell growth and differentiation, and cell death. TSC-22 is a putative transcriptional regulator containing a leucine zipper-like structure and a nuclear export signal. We previously showed the cytoplasmic localization of TSC-2...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:

    authors: Hino S,Kawamata H,Omotehara F,Uchida D,Begum NM,Yoshida H,Sato M,Fujimori T

    更新日期:2002-03-01 00:00:00

  • Changes of the O6-methylguanine-DNA methyltransferase promoter methylation and MGMT protein expression after adjuvant treatment in glioblastoma.

    abstract::The aim of this study was to evaluate variations of O6-methylguanine-DNA methyltransferase (MGMT) promoter methylation and protein expression after adjuvant treatment in glioblastoma patients. Sixteen patients with a glioblastoma underwent 34 microsurgeries including 18 re-operations. After surgery, patients underwent...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or_00000760

    authors: Jung TY,Jung S,Moon KS,Kim IY,Kang SS,Kim YH,Park CS,Lee KH

    更新日期:2010-05-01 00:00:00

  • A quadruple therapy synergistically blocks proliferation and promotes apoptosis of hepatoma cells.

    abstract::Effective therapy for advanced hepatocellular carcinoma (HCC) is lacking. Conventional chemotherapy was judged to be ineffective. We previously demonstrated that the histone deacetylase inhibitor Trichostatin A (TSA) blocks growth of HCC cells in vitro. The anti-tumoral effect of a combination of more than 2 classes o...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:

    authors: Ganslmayer M,Ocker M,Zopf S,Leitner S,Hahn EG,Schuppan D,Herold C

    更新日期:2004-05-01 00:00:00

  • Antiangiogenic effects of oxymatrine on pancreatic cancer by inhibition of the NF-κB-mediated VEGF signaling pathway.

    abstract::Oxymatrine, the main alkaloid component in the traditional Chinese herbal medicine Sophora japonica (Sophora flavescens Ait), has been reported to have antitumor properties. However, the mechanisms of action in human pancreatic cancer are not well established to date. In the present study, we investigated the antiangi...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or.2013.2529

    authors: Chen H,Zhang J,Luo J,Lai F,Wang Z,Tong H,Lu D,Bu H,Zhang R,Lin S

    更新日期:2013-08-01 00:00:00

  • The HIF‑1α/miR‑224‑3p/ATG5 axis affects cell mobility and chemosensitivity by regulating hypoxia‑induced protective autophagy in glioblastoma and astrocytoma.

    abstract::Human glioblastoma is a malignant and aggressive primary human brain solid tumor characterized by severe hypoxia. Hypoxia can induce autophagy, which may result in chemoresistance and malignant progression of cancer cells. MicroRNAs (miRNAs) have been reported to modulate hypoxia‑induced autophagy in various types of ...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or.2018.6929

    authors: Huang S,Qi P,Zhang T,Li F,He X

    更新日期:2019-03-01 00:00:00

  • Improved sensitivity and specificity for prostate cancer diagnosis based on the urine PCA3/PSA ratio acquired by sequence‑specific RNA capture.

    abstract::Prostate cancer antigen 3 (PCA3) is a non-coding RNA fragment that is overexpressed in prostate cancer cells. However, the clinical applications of PCA3 are highly limited due to the instability of RNA and the lack of reliable and efficient RNA extraction and purification methods. Thus, in the present study, we compar...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or.2015.4266

    authors: Zheng K,Dou Y,He L,Li H,Zhang Z,Chen Y,Ye A,Liu W,Kong L

    更新日期:2015-11-01 00:00:00

  • In vivo evidence for a significant role of folylpolyglutamate synthase in combined chemotherapy with oral fluoropyrimidine, UFT or S-1, and leucovorin.

    abstract::Combined chemotherapy with 5-fluorouracil and leucovorin (LV) has been widely used for the treatment of patients with colorectal cancer. Given that LV effects are attributable to increased levels of reduced folate in cancer cells, we attempted here to show the in vivo role of folylpolyglutamate synthetase (FPGS), whic...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or.2011.1206

    authors: Tsukioka S,Sakamoto E,Tsujimoto H,Nakagawa F,Saito H,Uchida J,Kiniwa M,Fukushima M

    更新日期:2011-05-01 00:00:00

  • MYC promotes the development of papillary thyroid carcinoma by inhibiting the expression of lncRNA PAX8‑AS1:28.

    abstract::As a common malignancy of the endocrine system, papillary thyroid carcinoma (PTC) seriously affects the quality of life of patients. lncRNA PAX8‑AS1:28, or lnc‑PSD4‑1:14 has been reported to be abnormally expressed in PTC. However, the function of PAX8‑AS1:28 in PTC is still unknown. Therefore, the present study aimed...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or.2019.6996

    authors: Zhang Y,Li F,Chen J

    更新日期:2019-04-01 00:00:00

  • Cancer-associated fibroblasts do not respond to combined irradiation and kinase inhibitor treatment.

    abstract::The emergence of radioresistance is a significant issue in the treatment of squamous cell carcinoma. We recently demonstrated that post-radiogenic extracellular signal-regulated kinase (ERK) signaling might decrease radiosensitivity in this cancer type. To further elucidate how tumo...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or.2012.2180

    authors: Affolter A,Schmidtmann I,Mann WJ,Brieger J

    更新日期:2013-02-01 00:00:00

  • Characterization of the microRNA profile in early-stage cervical squamous cell carcinoma by next-generation sequencing.

    abstract::Squamous cell carcinoma (SCC) is histologically the most prominent type of cervical cancer. There is accumulating evidence suggesting that microRNAs (miRNAs) play important regulatory roles in the biological processes of cervical squamous cell carcinoma (CSCC). Deciphering the miRNA regulatory network in CSCC could de...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or.2017.5372

    authors: Lin W,Feng M,Chen G,Zhou Z,Li J,Ye Y

    更新日期:2017-03-01 00:00:00

  • Ascorbate (vitamin C) induces cell death through the apoptosis-inducing factor in human breast cancer cells.

    abstract::Although ascorbate (Vitamin C) has been shown to inhibit cell growth and induce cell death in variety of cancer cells, results reported in other studies are inconsistent with this conclusion. It was previously reported that ascorbate induces apoptosis in human breast cancer cells. However, the molecular mechanism for ...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:

    authors: Hong SW,Jin DH,Hahm ES,Yim SH,Lim JS,Kim KI,Yang Y,Lee SS,Kang JS,Lee WJ,Lee WK,Lee MS

    更新日期:2007-10-01 00:00:00

  • Evaluation of the STAT3 inhibitor GLG‑302 for the prevention of estrogen receptor‑positive and ‑negative mammary cancers.

    abstract::Signal transducer and activator of transcription 3 (STAT3) plays a key role in the transformation of normal cells to cancerous cells. Although inhibitors of STAT3 have been shown to suppress the growth of multiple cancer types in vitro and in vivo, such agents are of particular interest for the prevention of breast ca...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or.2019.7219

    authors: Shoemaker RH,Fox JT,Juliana MM,Moeinpour FL,Grubbs CJ

    更新日期:2019-09-01 00:00:00

  • E26 transformation (ETS)‑specific related transcription factor‑3 (ELF3) orchestrates a positive feedback loop that constitutively activates the MAPK/Erk pathway to drive thyroid cancer.

    abstract::Thyroid cancer is the most commonly diagnosed malignancy of the endocrine system, the incidence of which has increased rapidly in the last 30 years. Genetic alterations in pathways, including the mitogen‑activated protein kinase (MAPK)/extracellular signal‑regulated kinase (Erk) and phosphatidylinositol‑3‑kinase (PI3K...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or.2018.6807

    authors: Chen H,Chen W,Zhang X,Hu L,Tang G,Kong J,Wang Z

    更新日期:2019-01-01 00:00:00

  • Inhibition of NADPH oxidase activity promotes differentiation of B16 melanoma cells.

    abstract::The activity of NADPH oxidase is increased in malignant skin keratinocytes. We demonstrated that inhibition of NADPH oxidase activity by diphenyleneiodonium (DPI) suppressed free radical production, inhibited cell growth and promoted cell differentiation of B16 melanoma cells, as indicated by cell morphology, increase...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:

    authors: Zhao Y,Liu J,McMartin KE

    更新日期:2008-05-01 00:00:00

  • Radiosensitization, after a combined treatment of survivin siRNA and irradiation, is correlated with the activation of caspases 3 and 7 in a wt-p53 sarcoma cell line, but not in a mt-p53 sarcoma cell line.

    abstract::Survivin, a member of the inhibitor-of-apoptosis family is an essential protein for regular mitosis and is involved in an anti-apoptotic pathway. In some studies, an association between survivin expression and radiosensitivity has been described for tumor cells, but the relationship between p53 and survivin regarding ...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:

    authors: Kappler M,Taubert H,Bartel F,Blümke K,Panian M,Schmidt H,Dunst J,Bache M

    更新日期:2005-01-01 00:00:00

  • MicroRNA-411 inhibits malignant biological behaviours of colorectal cancer cells by directly targeting PIK3R3.

    abstract::Colorectal cancer (CRC) is the third most common cancer and the fourth leading cause of cancer-related mortality worldwide. Aberrant expression of miRNAs play important roles in the development and progression of various types of cancers by modulating oncogenic and tumour-suppressor pathways. Therefore, exploring the ...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or.2017.6135

    authors: Zhao J,Xu J,Zhang R

    更新日期:2018-02-01 00:00:00

  • [Corrigendum] A 19‑miRNA Support Vector Machine classifier and a 6‑miRNA risk score system designed for ovarian cancer patients.

    abstract::Subsequently to the publication of this paper, the authors have realized that the affilitation address omitted the name of the university at which this research was conducted (Capital Medical University). Therefore, the author affiliations for this paper should have appeared as follows (changes are highlighted in bold...

    journal_title:Oncology reports

    pub_type: 杂志文章,已发布勘误

    doi:10.3892/or.2019.7385

    authors: Dong J,Xu M

    更新日期:2019-12-01 00:00:00

  • Identification of novel therapeutic targets in the secretome of ionizing radiation‑induced senescent tumor cells.

    abstract::Cellular senescence is a state of irreversible growth arrest that can be triggered by multiple mechanisms, including telomere shortening, the epigenetic derepression of the INK4α/ARF locus and DNA damage. Senescence has been considered a tumor‑suppressing mechanism that permanently arrests cells at risk for malignant ...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or.2015.4473

    authors: Hwang HJ,Jung SH,Lee HC,Han NK,Bae IH,Lee M,Han YH,Kang YS,Lee SJ,Park HJ,Ko YG,Lee JS

    更新日期:2016-02-01 00:00:00

  • S100A8 facilitates the migration of colorectal cancer cells through regulating macrophages in the inflammatory microenvironment.

    abstract::Previous studies have shown that S100 calcium-binding protein A8 (S100A8) contributes to the survival and migration of colorectal cancer (CRC) cells. However, whether S100A8 participates in the progression and metastasis of CRC via the regulation of macrophages in the tumor inflammatory microenvironment remains unknow...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or.2016.4790

    authors: Zha H,Sun H,Li X,Duan L,Li A,Gu Y,Zeng Z,Zhao J,Xie J,Yuan S,Li H,Zhou L

    更新日期:2016-07-01 00:00:00

  • MicroRNA-181d is a tumor suppressor in human esophageal squamous cell carcinoma inversely regulating Derlin-1.

    abstract::Esophageal squamous cell carcinoma (ESCC) is the predominant subtype of human esophageal cancer in East Asia. In the present study, we explored the tumor suppressive function of microRNA-181d (miR-181d) in ESCC. Quantitative RT-PCR was used to assess gene levels of miR‑181d in both ESCC cell lines and clinical samples...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or.2016.5028

    authors: Li D,Shi M,Ji H,Chen G,Jiang H,Wang Z

    更新日期:2016-10-01 00:00:00

  • Analysis of caspase activities in rat mammary tumours induced by N-methyl-nitrosourea.

    abstract::Normal breast development is controlled by a balance between cell proliferation and apoptosis. The balance between the two parameters is crucial for determining the growth or regression of breast tumours in response to therapies and treatments. Therefore, it is necessary to understand the role of apoptosis in tumour p...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:

    authors: Puertollano MA,Carrera MP,Puertollano E,Alvarez de Cienfuegos G,Ramirez-Expósito MJ,De Pablo MA,Martinez-Martos JM

    更新日期:2008-09-01 00:00:00