当前位置： SCI文献检索 > BIOMATERIALS期刊下所有文献 > Differential internalization of amphotericin B--conjugated nanoparticles in human cells and the expression of heat shock protein 70.

Differential internalization of amphotericin B--conjugated nanoparticles in human cells and the expression of heat shock protein 70.

Abstract：

:Although a variety of nanoparticles (NPs) functionalized with amphotericin B, an antifungal agent widely used in the clinic, have been studied in the last years their cytotoxicity profile remains elusive. Here we show that human endothelial cells take up high amounts of silica nanoparticles (SNPs) conjugated with amphotericin B (AmB) (SNP-AmB) (65.4 ± 12.4 pg of Si per cell) through macropinocytosis while human fibroblasts internalize relatively low amounts (2.3 ± 0.4 pg of Si per cell) because of their low capacity for macropinocytosis. We further show that concentrations of SNP-AmB and SNP up to 400 μg/mL do not substantially affect fibroblasts. In contrast, endothelial cells are sensitive to low concentrations of NPs (above 10 μg/mL), in particular to SNP-AmB. This is because of their capacity to internalize high concentration of NPs and high sensitivity of their membrane to the effects of AmB. Low-moderate concentrations of SNP-AmB (up to 100 μg/mL) induce the production of reactive oxygen species (ROS), LDH release, high expression of pro-inflammatory cytokines and chemokines (IL-8, IL-6, G-CSF, CCL4, IL-1β and CSF2) and high expression of heat shock proteins (HSPs) at gene and protein levels. High concentrations of SNP-AmB (above 100 μg/mL) disturb membrane integrity and kill rapidly human cells (60% after 5 h). This effect is higher in SNP-AmB than in SNP.

journal_name

Biomaterials

journal_title

Biomaterials

authors

Paulo CS,Lino MM,Matos AA,Ferreira LS

doi

10.1016/j.biomaterials.2013.03.048

subject

Has Abstract

pub_date

2013-07-01 00:00:00

pages

5281-93

issue

21

eissn

0142-9612

issn

1878-5905

pii

S0142-9612(13)00358-X

journal_volume

34

pub_type

杂志文章

相关文献

BIOMATERIALS文献大全
  • Cytotoxicity of silica nanoparticles through exocytosis of von Willebrand factor and necrotic cell death in primary human endothelial cells.

    abstract：:Nanoparticle-induced endothelial cell (EC) dysfunction, due to the induction of inflammation and/or the activation of the coagulation system, is associated with pulmonary and ischemic cardiovascular diseases. Although it is contigent on several mechanisms, involving formation of reactive oxygen species and inflammator...

    journal_title：Biomaterials

    pub_type： 杂志文章

    doi：10.1016/j.biomaterials.2011.07.078

    authors： Bauer AT,Strozyk EA,Gorzelanny C,Westerhausen C,Desch A,Schneider MF,Schneider SW

    更新日期：2011-11-01 00:00:00

  • The effect of adsorbed fibrinogen, fibronectin, von Willebrand factor and vitronectin on the procoagulant state of adherent platelets.

    abstract：:Procoagulant (activated) platelets provide a site for assembly of the prothrombinase complex which can rapidly convert prothrombin into thrombin (a potent inducer of clot formation). Previously, we reported that adhesion of platelets to surfaces preadsorbed with blood plasma caused them to become procoagulant. In the ...

    journal_title：Biomaterials

    pub_type： 杂志文章

    doi：10.1016/s0142-9612(00)00150-2

    authors： Grunkemeier JM,Tsai WB,McFarland CD,Horbett TA

    更新日期：2000-11-01 00:00:00

  • The roles of tissue engineering and vascularisation in the development of micro-vascular networks: a review.

    abstract：:The construction of tissue-engineered devices for medical applications is now possible in vitro using cell culture and bioreactors. Although methods of incorporating them back into the host are available, current constructs depend purely on diffusion which limits their potential. The absence of a vascular network capa...

    journal_title：Biomaterials

    pub_type： 杂志文章,评审

    doi：10.1016/j.biomaterials.2004.07.006

    authors： Kannan RY,Salacinski HJ,Sales K,Butler P,Seifalian AM

    更新日期：2005-05-01 00:00:00

  • Two-dimensional nanosheets with high curcumin loading content for multimodal imaging-guided combined chemo-photothermal therapy.

    abstract：:Nowadays, two-dimensional (2D) nanomaterials with many fascinating physicochemical properties have drawn extensive attention as drug delivery platforms for cancer theranostics. Nevertheless, current existing 2D nanomaterial-based drug delivery systems normally undergo the bottlenecks of hash preparation process, low d...

    journal_title：Biomaterials

    pub_type： 杂志文章

    doi：10.1016/j.biomaterials.2019.119470

    authors： Liu F,Lin L,Zhang Y,Sheng S,Wang Y,Xu C,Tian H,Chen X

    更新日期：2019-12-01 00:00:00

  • Wound treatment with Sorbsan--an alginate fibre dressing.

    abstract：:This highly absorbent, biodegradable dressing derived from seaweed can be successfully applied to cleanse a wide variety of secreting lesions. The high absorption of exudate is achieved via strong hydrophilic gel formation and this serves to control wound secretion levels and to minimize bacterial contamination. Algin...

    journal_title：Biomaterials

    pub_type： 杂志文章

    doi：10.1016/0142-9612(83)90036-4

    authors： Gilchrist T,Martin AM

    更新日期：1983-10-01 00:00:00

  • Thermal behaviour of hydroxyapatite intended for medical applications.

    abstract：:Four commercial hydroxyapatites (both natural and synthetic) were tested to assess transformations of the chemical and crystalline structure following variation of temperature from 20 to 1600 degrees C. The thermal behaviour of hydroxyapatite is relevant for biomedical applications such as plasma spraying of metallic ...

    journal_title：Biomaterials

    pub_type： 杂志文章

    doi：10.1016/0142-9612(93)90146-s

    authors： Locardi B,Pazzaglia UE,Gabbi C,Profilo B

    更新日期：1993-05-01 00:00:00

  • Targeting dendritic cells in lymph node with an antigen peptide-based nanovaccine for cancer immunotherapy.

    abstract：:The design of peptide-based subunit vaccine formulations for the direct delivery of tumor antigen peptides (Aps) to dendritic cells (DCs) localized within draining lymph nodes (DLNs) is challenging. Mature DCs (mDCs) are abundantly distributed within DLNs but have dramatically reduced endocytic uptake and antigen-proc...

    journal_title：Biomaterials

    pub_type： 杂志文章

    doi：10.1016/j.biomaterials.2016.05.008

    authors： Qian Y,Jin H,Qiao S,Dai Y,Huang C,Lu L,Luo Q,Zhang Z

    更新日期：2016-08-01 00:00:00

  • Electrospun degradable polyesterurethane membranes: potential scaffolds for skeletal muscle tissue engineering.

    abstract：:Skeletal muscle tissue engineering represents an attractive approach to overcome problems associated with autologous transfer of muscle tissue and provides a valid alternative in muscle regeneration enhancement. The aim of this study was to investigate the suitability, as scaffold for skeletal muscle tissue engineerin...

    journal_title：Biomaterials

    pub_type： 杂志文章

    doi：10.1016/j.biomaterials.2004.11.035

    authors： Riboldi SA,Sampaolesi M,Neuenschwander P,Cossu G,Mantero S

    更新日期：2005-08-01 00:00:00

  • Local variation in the tearing strength of chemically modified pericardium.

    abstract：:Forty squares of tissue, 40 mm X 40 mm, were harvested from bovine pericardium fixed in glutaraldehyde free of tension. Two parallel dumb-bell specimens were excised from each square. In 20 pairs of adjacent samples, two 1 mm cuts were introduced into one of the samples at the centre of the dumb-bell. The other 20 pai...

    journal_title：Biomaterials

    pub_type： 杂志文章

    doi：10.1016/0142-9612(89)90098-7

    authors： Crofts CE,Trowbridge EA

    更新日期：1989-05-01 00:00:00

  • The influence of skeletal maturity on allogenic synovial mesenchymal stem cell-based repair of cartilage in a large animal model.

    abstract：:One of the potential factors that may affect the results of mesenchymal stem cell (MSC)-based therapy is the age of donors and recipients. However, there have been no controlled studies to investigate the influence of skeletal maturity on the MSC-based repair of cartilage. The purpose of this study was to compare the ...

    journal_title：Biomaterials

    pub_type： 杂志文章

    doi：10.1016/j.biomaterials.2010.07.017

    authors： Shimomura K,Ando W,Tateishi K,Nansai R,Fujie H,Hart DA,Kohda H,Kita K,Kanamoto T,Mae T,Nakata K,Shino K,Yoshikawa H,Nakamura N

    更新日期：2010-11-01 00:00:00

  • Three-dimensional, soft neotissue arrays as high throughput platforms for the interrogation of engineered tissue environments.

    abstract：:Local signals from tissue-specific extracellular matrix (ECM) microenvironments, including matrix adhesive ligand, mechanical elasticity and micro-scale geometry, are known to instruct a variety of stem cell differentiation processes. Likewise, these signals converge to provide multifaceted, mechanochemical cues for h...

    journal_title：Biomaterials

    pub_type： 杂志文章

    doi：10.1016/j.biomaterials.2015.04.036

    authors： Floren M,Tan W

    更新日期：2015-08-01 00:00:00

  • Preparation of CM-chitin microspheres by complexation with iron(III) in w/o emulsion and their biodisposition characteristics in mice.

    abstract：:6-O-Carboxymethylchitin (CM-chitin) was partially deacetylated by alkaline treatment, and the 30% deacetylated product was named CM-DA30 and used for the preparation of microspheres. An aqueous solution of CM-DA30 was added to hexane containing 1% (w/w) sorbitan sesquioleate and emulsified by stirring and sonication, ...

    journal_title：Biomaterials

    pub_type： 杂志文章

    doi：10.1016/s0142-9612(00)00069-7

    authors： Hata H,Onishi H,Machida Y

    更新日期：2000-09-01 00:00:00

  • Antibody and cytokine-associated immune responses to S. equi antigens entrapped in PLA nanospheres.

    abstract：:Strangles is an infectious disease caused by Streptococcus equi subspecies equi that affects the upper respiratory tract of the Equidae. The control of this disease seems to be dependent on its earlier detection and prevention, but prolonged animal protection without development of strong and severe side effects has n...

    journal_title：Biomaterials

    pub_type： 杂志文章

    doi：10.1016/j.biomaterials.2009.05.045

    authors： Florindo HF,Pandit S,Gonçalves LM,Videira M,Alpar O,Almeida AJ

    更新日期：2009-10-01 00:00:00

  • Biological evaluation of an ionomeric bone cement by osteoblast cell culture methods.

    abstract：:Periosteal derived bovine osteoblast-like cells migrated in culture onto an ionomeric cement. Cell cultures were maintained for 4 weeks and used to study the in vitro behaviour of cells on the ionomeric bone cement (IC). The cells produced bone matrix proteins (osteocalcin, bone sialoprotein II) and were osteoblast-li...

    journal_title：Biomaterials

    pub_type： 杂志文章

    doi：10.1016/0142-9612(93)90133-m

    authors： Meyer U,Szulczewski DH,Barckhaus RH,Atkinson M,Jones DB

    更新日期：1993-10-01 00:00:00

  • Adsorption of amphiphilic hyperbranched polyglycerol derivatives onto human red blood cells.

    abstract：:Hydrophobically derivatized hyperbranched polyglycerol (HPG)-polyethylene glycol (PEG) polymers bearing stearoyl chains (HPG-C18-PEG) were originally developed as human serum albumin substitutes and further as a unimolecular drug delivery system. In view of these in vivo applications and the potential for membrane int...

    journal_title：Biomaterials

    pub_type： 杂志文章

    doi：10.1016/j.biomaterials.2010.01.021

    authors： Liu Z,Janzen J,Brooks DE

    更新日期：2010-04-01 00:00:00

  • Receptor tyrosine kinases-instructed release of its inhibitor from hydrogel to delay ovarian aging.

    abstract：:Premature ovarian failure (POF) is the most frequently occurred disease in ovary. Direct inhibition of mammalian target of rapamycin (mTOR) activity can treat woman POF but brings adverse effects to women. Herein, by rational design of a hydrogelator Nap-Phe-Phe-Asp-Arg-Leu-Tyr-OH (Y) and co-assembling Y with an inhib...

    journal_title：Biomaterials

    pub_type： 杂志文章

    doi：10.1016/j.biomaterials.2020.120536

    authors： Shi Z,Li X,Wei M,Chen P,Zhang T,Ling X,Zhang J,Zhao C,Wang F,Liang G

    更新日期：2020-11-16 00:00:00

  • The effect of platelet lysate supplementation of a dextran-based hydrogel on cartilage formation.

    abstract：:In situ gelating dextran-tyramine (Dex-TA) injectable hydrogels have previously shown promising features for cartilage repair. Yet, despite suitable mechanical properties, this system lacks intrinsic biological signals. In contrast, platelet lysate-derived hydrogels are rich in growth factors and anti-inflammatory cyt...

    journal_title：Biomaterials

    pub_type： 杂志文章

    doi：10.1016/j.biomaterials.2012.01.051

    authors： Moreira Teixeira LS,Leijten JC,Wennink JW,Chatterjea AG,Feijen J,van Blitterswijk CA,Dijkstra PJ,Karperien M

    更新日期：2012-05-01 00:00:00

  • Lymph-directed nitric oxide increases immune cell access to lymph-borne nanoscale solutes.

    abstract：:Lymph nodes (LNs) are immune organs housing high concentrations of lymphocytes, making them critical targets for therapeutic immunomodulation in a wide variety of diseases. While there is great interest in targeted drug delivery to LNs, many nanoscale drug delivery carriers have limited access to parenchymal resident ...

    journal_title：Biomaterials

    pub_type： 杂志文章

    doi：10.1016/j.biomaterials.2020.120411

    authors： Sestito LF,Thomas SN

    更新日期：2021-01-01 00:00:00

  • 2-Methacryloyloxyethyl phosphorylcholine polymer (MPC)-coating improves the transfection activity of GALA-modified lipid nanoparticles by assisting the cellular uptake and intracellular dissociation of plasmid DNA in primary hepatocytes.

    abstract：:We previously reported that modification of GALA peptide on the surface of liposomes enhanced fusion with endosomal membrane, and cytoplasmic release of encapsulated macromolecules. We report herein that an additional coating of GALA-modified liposomes with 2-methacryloyloxyethyl phosphorylcholine (MPC) polymer result...

    journal_title：Biomaterials

    pub_type： 杂志文章

    doi：10.1016/j.biomaterials.2010.04.031

    authors： Ukawa M,Akita H,Masuda T,Hayashi Y,Konno T,Ishihara K,Harashima H

    更新日期：2010-08-01 00:00:00

  • Preparation of EVAL membranes with smooth and particulate morphologies for neuronal culture.

    abstract：:In this work, the in vitro interaction of cerebellar granule neurons prepared from 7-day-old Wistar rats and poly ethylene-co-vinyl alcohol (EVAL) membranes was investigated. Cells were cultured in smooth and particulate EVAL membranes for up to 7 days. Particulate membranes were prepared by using 1-octanol to precipi...

    journal_title：Biomaterials

    pub_type： 杂志文章

    doi：10.1016/s0142-9612(00)00337-9

    authors： Young TH,Lin CW,Cheng LP,Hsieh CC

    更新日期：2001-07-01 00:00:00

  • Extracellular matrix deposition of bone marrow stroma enhanced by macromolecular crowding.

    abstract：:Decellularized extracellular matrices (ECM) from in vitro cell cultures can serve as in vivo-like matrix scaffolds for modulating cell-ECM interactions. Macromolecular crowding (MMC), the supplementation of synthetic or naturally occurring molecules resulting in excluded volume effects (EVE), has been demonstrated to ...

    journal_title：Biomaterials

    pub_type： 杂志文章

    doi：10.1016/j.biomaterials.2015.09.014

    authors： Prewitz MC,Stißel A,Friedrichs J,Träber N,Vogler S,Bornhäuser M,Werner C

    更新日期：2015-12-01 00:00:00

  • Establishing the protein MIA (melanoma inhibitory activity) as a marker for chondrocyte differentiation.

    abstract：:Melanoma inhibitory activity (MIA), also referred to as cartilage derived retinoic acid-sensitive protein (CD-RAP), is detected physiologically in cartilage tissue and pathologically in malignant melanomas. To measure MIA/CD-RAP quantitatively we developed a sensitive ELISA system. Recently, we described diagnostic ap...

    journal_title：Biomaterials

    pub_type： 杂志文章

    doi：10.1016/s0142-9612(03)00184-4

    authors： Bosserhoff AK,Buettner R

    更新日期：2003-08-01 00:00:00

  • Volumetric interpretation of protein adsorption: interfacial packing of protein adsorbed to hydrophobic surfaces from surface-saturating solution concentrations.

    abstract：:The maximum capacity of a hydrophobic adsorbent is interpreted in terms of square or hexagonal (cubic and face-centered-cubic, FCC) interfacial packing models of adsorbed blood proteins in a way that accommodates experimental measurements by the solution-depletion method and quartz-crystal-microbalance (QCM) for the h...

    journal_title：Biomaterials

    pub_type： 杂志文章

    doi：10.1016/j.biomaterials.2010.09.075

    authors： Kao P,Parhi P,Krishnan A,Noh H,Haider W,Tadigadapa S,Allara DL,Vogler EA

    更新日期：2011-02-01 00:00:00

  • Hyaluronic acid hydrogel in the treatment of osteoarthritis.

    abstract：:In order to overcome the problem of rapid clearance of the polysaccharide hyaluronic acid (Hyal) in the treatment of osteoarthritis (OA), a 50% cross-linked Hyal hydrogel (Hyal 50%) was synthesised. The 50% refers to the amount of COOH groups of the polysaccharide involved in the cross-linking reaction. i.e. 50% of th...

    journal_title：Biomaterials

    pub_type： 杂志文章

    doi：10.1016/s0142-9612(02)00194-1

    authors： Barbucci R,Lamponi S,Borzacchiello A,Ambrosio L,Fini M,Torricelli P,Giardino R

    更新日期：2002-12-01 00:00:00

  • Tumor-activatable ultrasmall nanozyme generator for enhanced penetration and deep catalytic therapy.

    abstract：:Tumor-activatable ultrasmall nanozyme generation is an unprecedented strategy to overcome intrinsically fatal defects of traditional reactive oxygen species (ROS)-based nanoagents for deep tumor penetration, including limited tissue-penetrating depth of external energy, heavy reliance on oxygen and nonspecific toxicit...

    journal_title：Biomaterials

    pub_type： 杂志文章

    doi：10.1016/j.biomaterials.2020.120263

    authors： Liu X,Liu Z,Dong K,Wu S,Sang Y,Cui T,Zhou Y,Ren J,Qu X

    更新日期：2020-11-01 00:00:00

  • The use of chitosan as a condensing agent to enhance emulsion-mediated gene transfer.

    abstract：:Previously we have formulated a new cationic emulsion, composed of 3beta [N-(N',N'-dimethylaminoethane) carbamoyl] cholesterol and dioleoylphosphatidyl ethanolamine, castor oil and Tween 80, and it efficiently delivered plasmid DNA into various cancer cells with low toxicity. Chitosan is a natural cationic polysacchar...

    journal_title：Biomaterials

    pub_type： 杂志文章

    doi：10.1016/j.biomaterials.2004.07.008

    authors： Lee MK,Chun SK,Choi WJ,Kim JK,Choi SH,Kim A,Oungbho K,Park JS,Ahn WS,Kim CK

    更新日期：2005-05-01 00:00:00

  • Effects of clinically relevant alumina ceramic wear particles on TNF-alpha production by human peripheral blood mononuclear phagocytes.

    abstract：:The recent introduction of microseparation of the components of ceramic-on-ceramic hip prostheses during hip simulations has produced clinically relevant wear rates, wear patterns and wear particles. This provided an opportunity to determine the response of primary human peripheral blood mononuclear cells to clinicall...

    journal_title：Biomaterials

    pub_type： 杂志文章

    doi：10.1016/s0142-9612(02)00510-0

    authors： Hatton A,Nevelos JE,Matthews JB,Fisher J,Ingham E

    更新日期：2003-03-01 00:00:00

  • Tissue response to polyanionic collagen: elastin matrices implanted in rat calvaria.

    abstract：:The tissue response to polyanionic collagen matrices, prepared from bovine pericardium and implanted subperiosteally in rat calvaria, was studied. The materials were implanted in 72 male rats (Rattus norvegicus, albinus, Holtzman), randomly divided into four groups: GI-MBP hydrolyzed for 24 h; GII-MBP hydrolyzed for 3...

    journal_title：Biomaterials

    pub_type： 杂志文章

    doi：10.1016/s0142-9612(02)00292-2

    authors： Rosa FP,Lia RC,de Souza KO,Goissis G,Marcantonio E Jr

    更新日期：2003-01-01 00:00:00

  • Chondroitin sulphate-based 3D scaffolds containing MWCNTs for nervous tissue repair.

    abstract：:Nervous tissue lesions are an important social concern due to their increasing prevalence and their high sanitary costs. Their treatment still remains a challenge because of the reduced ability of nervous tissue to regenerate, its intrinsic structural and functional complexity and the rapid formation of fibroglial sca...

    journal_title：Biomaterials

    pub_type： 杂志文章

    doi：10.1016/j.biomaterials.2013.11.017

    authors： Serrano MC,Nardecchia S,García-Rama C,Ferrer ML,Collazos-Castro JE,del Monte F,Gutiérrez MC

    更新日期：2014-02-01 00:00:00

  • Degradable poly(anhydride ester) implants: effects of localized salicylic acid release on bone.

    abstract：:Degradable poly(anhydride ester) implants in which the polymer backbone breaks down into salicylic acid (SA) were investigated. In this preliminary work, local release of SA from the poly(anhydride esters), thus classified as 'active polymers', on healthy bone and tissue was evaluated in vivo using a mouse model. Degr...

    journal_title：Biomaterials

    pub_type： 杂志文章

    doi：10.1016/s0142-9612(00)00118-6

    authors： Erdmann L,Macedo B,Uhrich KE

    更新日期：2000-12-01 00:00:00