Abstract：
:The role of the collagen-platelet interaction is of crucial importance to the haemostatic response during both injury and pathogenesis of the blood vessel wall. Of particular interest is the high affinity interaction of the platelet transmembrane receptor, alpha 2 beta 1, responsible for firm attachment of platelets to collagen at and around injury sites. We employ single molecule force spectroscopy (SMFS) using the atomic force microscope (AFM) to study the interaction of the I-domain from integrin alpha 2 beta 1 with a synthetic collagen related triple-helical peptide containing the high-affinity integrin-binding GFOGER motif, and a control peptide lacking this sequence, referred to as GPP. By utilising synthetic peptides in this manner we are able to study at the molecular level subtleties that would otherwise be lost when considering cell-to-collagen matrix interactions using ensemble techniques. We demonstrate for the first time the complexity of this interaction as illustrated by the complex multi-peaked force spectra and confirm specificity using control blocking experiments. In addition we observe specific interaction of the GPP peptide sequence with the I-domain. We propose a model to explain these observations.
journal_name
Int J Mol Scijournal_title
International journal of molecular sciencesauthors
Attwood SJ,Simpson AM,Hamaia SW,Bihan D,Roy D,Farndale RW,Welland MEdoi
10.3390/ijms14022832subject
Has Abstractpub_date
2013-01-29 00:00:00pages
2832-45issue
2issn
1422-0067pii
ijms14022832journal_volume
14pub_type
杂志文章abstract：:Platycodin D (PD), a major saponin (platycoside) in Platycodi radix (balloon flower root), has higher pharmacological activity than the other major platycosides; however, its content in the plant root is only approximately 10% (w/w) and the productivities of PD by several enzymes are still too low for industrial appli...
journal_title：International journal of molecular sciences
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abstract：:Cytokine-induced killer (CIK) cells are heterogeneous, major histocompatibility complex (MHC)-unrestricted T lymphocytes that have acquired the expression of several natural killer (NK) cell surface markers following the addition of interferon gamma (IFN-γ), OKT3 and interleukin-2 (IL-2). Treatment with CIK cells demo...
journal_title：International journal of molecular sciences
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journal_title：International journal of molecular sciences
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journal_title：International journal of molecular sciences
pub_type： 杂志文章
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journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
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journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
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journal_title：International journal of molecular sciences
pub_type： 杂志文章
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更新日期：2016-01-13 00:00:00
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journal_title：International journal of molecular sciences
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journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
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更新日期：2016-05-05 00:00:00
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journal_title：International journal of molecular sciences
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更新日期：2020-09-20 00:00:00
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journal_title：International journal of molecular sciences
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更新日期：2017-07-07 00:00:00
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journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
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更新日期：2019-03-25 00:00:00
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journal_title：International journal of molecular sciences
pub_type： 杂志文章
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更新日期：2019-04-26 00:00:00
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journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
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更新日期：2018-11-29 00:00:00
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journal_title：International journal of molecular sciences
pub_type： 杂志文章
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更新日期：2012-01-01 00:00:00
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journal_title：International journal of molecular sciences
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更新日期：2020-02-07 00:00:00
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journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms14010226
更新日期：2012-12-21 00:00:00
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journal_title：International journal of molecular sciences
pub_type： 杂志文章,多中心研究,随机对照试验
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更新日期：2016-07-02 00:00:00
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journal_title：International journal of molecular sciences
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journal_title：International journal of molecular sciences
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journal_title：International journal of molecular sciences
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journal_title：International journal of molecular sciences
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更新日期：2020-08-04 00:00:00
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journal_title：International journal of molecular sciences
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journal_title：International journal of molecular sciences
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更新日期：2012-11-22 00:00:00
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journal_title：International journal of molecular sciences
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更新日期：2019-05-27 00:00:00
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journal_title：International journal of molecular sciences
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更新日期：2020-11-11 00:00:00
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journal_title：International journal of molecular sciences
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更新日期：2018-10-26 00:00:00
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journal_title：International journal of molecular sciences
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更新日期：2020-08-31 00:00:00
abstract：:Transcriptome profiling of 3D models compared to 2D models in various cancer cell lines shows differential expression of TGF-β-mediated and cell adhesion pathways. Presence of TGF-β in these cell lines shows an increased invasion potential which is specific to cell type. In the present study, we identified exogenous a...
journal_title：International journal of molecular sciences
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更新日期：2019-07-22 00:00:00
