Abstract:
:Better understanding of protein higher order structures (HOS) is of major interest to researchers in the field of biotechnology and biopharmaceutics. Monitoring a protein's HOS is crucial towards understanding the impact of molecular conformation on the biotechnological application. In addition, maintaining the HOS is critical for achieving robust processes and developing stable formulations of therapeutic proteins. Loss of HOS contributes to increased aggregation, enhanced immunogenicity and loss of function. Selecting the proper biophysical methods to monitor the secondary and tertiary structures of therapeutic proteins remains the central question in this field. In this study, both Fourier Transform Infrared (FTIR) and vibrational circular dichroism (VCD) spectroscopy are employed to characterize the secondary structures of various proteins as a function of temperature and pH. Three proteins with different secondary structures were examined, human serum albumin (HSA), myoglobin, and the monoclonal antibody, ofatumumab. This work demonstrates that VCD is useful technique for monitoring subtle secondary structure changes of protein therapeutics that may occur during processing or handling.
journal_name
Curr Pharm Biotechnoljournal_title
Current pharmaceutical biotechnologyauthors
Nagarkar RP,Murphy BM,Yu X,Manning MC,Al-Azzam WAdoi
10.2174/1389201011314020010subject
Has Abstractpub_date
2013-01-01 00:00:00pages
199-208issue
2eissn
1389-2010issn
1873-4316pii
CPB-EPUB-20121116-1journal_volume
14pub_type
杂志文章abstract::Many cationic peptides with antimicrobial properties have been isolated from bacteria, fungi, plants, and animals. These peptides vary in molecular size, potency and spectra of activities. This report surveyed the literature to highlight the peptides that have antifungal activity and greatest potential for development...
journal_title:Current pharmaceutical biotechnology
pub_type: 杂志文章,评审
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journal_title:Current pharmaceutical biotechnology
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journal_title:Current pharmaceutical biotechnology
pub_type: 杂志文章,评审
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journal_title:Current pharmaceutical biotechnology
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journal_title:Current pharmaceutical biotechnology
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journal_title:Current pharmaceutical biotechnology
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journal_title:Current pharmaceutical biotechnology
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journal_title:Current pharmaceutical biotechnology
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journal_title:Current pharmaceutical biotechnology
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journal_title:Current pharmaceutical biotechnology
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更新日期:2011-07-01 00:00:00
abstract:BACKGROUND:Herein, we report the biosynthesis procedure to prepare silver nanoparticles as reduction and capping agents with the aqueous plant extract of Perovskia abrotanoides. METHODS:The therapeutic application of silver nanoparticles entirely depends on the size and shape of the nanoparticles therefore, their cont...
journal_title:Current pharmaceutical biotechnology
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journal_title:Current pharmaceutical biotechnology
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abstract::3,4-Methylenedioxymethamphetamine (MDMA or ecstasy) is a worldwide illegally used amphetamine-derived designer drug known to be hepatotoxic to humans. Jaundice, hepatomegaly, centrilobular necrosis, hepatitis and fibrosis represent some of the adverse effects caused by MDMA in the liver. Although there is irrefutable ...
journal_title:Current pharmaceutical biotechnology
pub_type: 杂志文章,评审
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更新日期:2010-08-01 00:00:00
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journal_title:Current pharmaceutical biotechnology
pub_type: 杂志文章,评审
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更新日期:2008-06-01 00:00:00
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journal_title:Current pharmaceutical biotechnology
pub_type: 杂志文章,评审
doi:10.2174/1389201013378581
更新日期:2001-12-01 00:00:00
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journal_title:Current pharmaceutical biotechnology
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更新日期:2013-01-01 00:00:00
abstract::The purpose of this review article is to describe the history of photodynamic therapy (PDT), its current medical applications, the mechanism of action, contraindications of the method, and different types of photosensitizers used. The second part of the article deals with applications for gastrointestinal diseases. Th...
journal_title:Current pharmaceutical biotechnology
pub_type: 杂志文章,评审
doi:10.2174/1389201043376805
更新日期:2004-08-01 00:00:00
abstract::Ivermectin is a broad spectrum antiparasitic veterinary drug introduced in human medicine in 1987. It is considered the drug of choice in onchocerciasis and strongyloidiasis infections, and remains as a therapeutic option for mass treatment in lymphatic filariasis, for which it has widely proved its efficacy. While re...
journal_title:Current pharmaceutical biotechnology
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更新日期:2012-05-01 00:00:00
abstract::Signals relayed through G protein-coupled receptors (GPCR) play pivotal roles in human physiology and are important drug targets. About 40% of all GPCRs couple to the heterotrimeric G protein Gq. Biochemical studies as well as crystallography have improved our understanding of GqPCRs and their downstream partners. Her...
journal_title:Current pharmaceutical biotechnology
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journal_title:Current pharmaceutical biotechnology
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journal_title:Current pharmaceutical biotechnology
pub_type: 杂志文章,评审
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更新日期:2005-04-01 00:00:00
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journal_title:Current pharmaceutical biotechnology
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更新日期:2009-09-01 00:00:00
abstract:BACKGROUND:`Herbal mixtures` containing synthetic cannabinoid receptor agonists (SCRAs) are promoted as legal alternative to marihuana and are easily available via the Internet. Keeping analytical methods for the detection of these SCRAs up-to-date is a continuous challenge for clinicians and toxicologists due to the h...
journal_title:Current pharmaceutical biotechnology
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更新日期:2018-01-01 00:00:00
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journal_title:Current pharmaceutical biotechnology
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更新日期:2013-01-01 00:00:00
abstract::Gene therapy is a potential treatment for severe inherited disorders for which there is little hope of finding a conventional cure. These include lethal diseases like immunodeficiencies and metabolic disorders, and non lethal conditions associated to poor quality of life and life-long symptomatic treatments, like musc...
journal_title:Current pharmaceutical biotechnology
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更新日期:2012-08-01 00:00:00
abstract:BACKGROUND:Many health hazardous diseases are caused by clinical pathogens. Drugresistant microbes are one of the major health problems in the world. To overcome the effect of infectious diseases new antimicrobial agent from nature has been explored which is environmentally friendly, less costly and more effective for ...
journal_title:Current pharmaceutical biotechnology
pub_type: 信件
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更新日期:2018-01-01 00:00:00
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journal_title:Current pharmaceutical biotechnology
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更新日期:2015-01-01 00:00:00
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journal_title:Current pharmaceutical biotechnology
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更新日期:2011-05-01 00:00:00
abstract:BACKGROUND:Up to 25% of diabetic patients will develop a diabetic foot ulcer. Chronic wounds such as diabetic foot ulcers often fail to heal with conventional therapies. In recent years, it has been identified that chronic wounds are usually associated with elevated level of matrix metalloproteinases (MMPs). Doxycyclin...
journal_title:Current pharmaceutical biotechnology
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更新日期:2017-01-01 00:00:00