In vitro Studies on Cytotoxic, DNA Protecting, Antibiofilm and Antibacterial Effects of Biogenic Silver Nanoparticles Prepared with Bergenia ciliata Rhizome Extract.

Abstract:

BACKGROUND:Many health hazardous diseases are caused by clinical pathogens. Drugresistant microbes are one of the major health problems in the world. To overcome the effect of infectious diseases new antimicrobial agent from nature has been explored which is environmentally friendly, less costly and more effective for the development of next-generation drugs. Bergenia ciliata and silver nitrate both have medicinal properties. OBJECTIVES:The aim of the current research was to evaluate the cytotoxic, and antibacterial effect of green synthesized nanoparticles using Bergenia ciliata rhizome against clinical bacterial pathogens. METHODS:Extract of Bergenia ciliata was prepared by maceration technique. Silver nanoparticles were synthesized using Bergenia ciliata rhizome extract. Synthesized silver nanoparticles were confirmed by UV-vis spectrophotometer, Scanning electron microscope (SEM) and Fourier Transform Infrared Spectroscopy (FTIR). The antibacterial, anti-biofilm, cell proliferation inhibition, DNA protection, brine shrimp lethality effects of synthesized nanoparticles were investigated. RESULTS:UV-vis spectrophotometer indicated the prelaminar synthesis of silver nanoparticles at 400 nm. The spherical shape of synthesized nanoparticles with 35 nm size was confirmed using SEM. Greatest zone of inhibition (6.0 ± 0.0 mm to 8.3 ± 0.57 mm) was recorded against all tested pathogens compared with the B. ciliata aqueous extract. Anti-biofilm analysis and MTT assay supported the results of the antibacterial activity. Silver nanoparticles protect the DNA degradation. CONCLUSION:Green synthesized nanoparticles had potent antibacterial activity and may provide a basis for the development of the new antibacterial drug.

journal_name

Curr Pharm Biotechnol

authors

Zia G,Sadia H,Nazir S,Ejaz K,Ali S,Ihsan-Ul-Haq,Iqbal T,Khan MAR,Raza A,Andleeb S

doi

10.2174/1389201019666180417160049

subject

Has Abstract

pub_date

2018-01-01 00:00:00

pages

68-78

issue

1

eissn

1389-2010

issn

1873-4316

pii

CPB-EPUB-89790

journal_volume

19

pub_type

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