当前位置： SCI文献检索 > INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES期刊下所有文献 > Biosynthesis and characterization of polyhydroxyalkanoates by Pseudomonas guezennei from alkanoates and glucose.

Biosynthesis and characterization of polyhydroxyalkanoates by Pseudomonas guezennei from alkanoates and glucose.

Abstract：

:The biosynthesis of medium chain length poly(3-hydroxyalkanoates) mcl PHAs by Pseudomonas guezennei using glucose, sodium octanoate, and 10-undecenoic acid as sole or mixed carbon sources was investigated. Chemical composition of polyesters was analyzed by GCMS and NMR. The copolyester produced by P. guezennei from glucose mainly consisted of 3-hydroxyoctanoate and 3-hydroxydecanoate, and the presence of 3-hydroxydodec-5-enoate was demonstrated. Using sodium octanoate as the sole nutrient, the microorganism produced a poly(3-hydroxyoctanoate) (PHO) polymer containing up to 94 mol% 3-hydroxyoctanoate. Biosynthesis of poly[(3-hydroxyoctanoate)-co-(3-hydroxyundecenoate)] (PHOU) copolymers bearing terminal reactive double bonds on its side chains with unsaturation degree ranging from 8.8% to 78.2% was obtained by tuning the ratio of sodium octanoate/10-undecenoic acid in the medium. Thermal analysis indicated semi-crystalline polymers with melting temperatures (T(m)) ranging from 46 to 55°C, fusion enthalpy (ΔH) comprised between 3 and 35 J/g and glass transition temperature (T(g)) from -36 to -44°C, except for the highly amorphous 78.2% unsaturated PHOU with a low T(g) (-50°C). Molecular weights determined by GPC ranged from 119000 and 530000 g/mol. The biosynthesis of natural polyesters with controlled ratio of vinyl-terminated side chains is of great interest for further chemical modifications.

journal_name

Int J Biol Macromol

journal_title

International journal of biological macromolecules

authors

Simon-Colin C,Gouin C,Lemechko P,Schmitt S,Senant A,Kervarec N,Guezennec J

doi

10.1016/j.ijbiomac.2012.08.018

subject

Has Abstract

pub_date

2012-12-01 00:00:00

pages

1063-9

issue

5

eissn

0141-8130

issn

1879-0003

pii

S0141-8130(12)00334-0

journal_volume

51

pub_type

杂志文章

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