Abstract:
:Preeclampsia is a multisystem disorder with profound implications on both mother and fetus. Analysis of umbilical cord blood red blood cell (RBC) changes shall depict the fetal response to pregnancy-specific complications like preeclampsia. This study aims to analyze the regulation relationship between HSP90α and heme oxygenase-2 (HO-2) in cord blood RBC during preeclampsia. The lipid hydroperoxide (LHP) and 3-nitrotyrosine (3-NT) levels were measured as stress markers in cord blood RBC of both subjects. The impact of stress on RBC was assessed by measuring the level of membrane bound enzymes and assessing the changes in cord blood RBC. The expression of HSP90α and HO-2 were analyzed by enzyme-linked immunosorbent assay (ELISA) and immunohistochemistry analysis, respectively. There was significant increase in the level of LHP (p < 0.01), 3-NT (p < 0.05), HSP90α (p < 0.01) with decrease in the expression of HO-2 (p < 0.05) in cord blood RBC of preeclamptic subjects compared to normotensive subjects. Similarly, the membrane damage in preeclamptic RBC was assessed by spectrophotometrically and found to be increased by 41.7%, along with increase in number of nucleated RBC. The antiproliferative effect of carbon monoxide under stress might decrease the expression of HO-2 under conditions when there is an increasing need for RBC. The role of HSP90α level in cord blood RBC is discussed with reference to nitrative stress in preeclampsia. This study concludes the increased expression of nucleated RBC, HSP90α and corresponding decreased expression of HO-2 in such hypoxic condition may play a protective role; to prevent cord blood RBC against stress induced damage during preeclampsia.
journal_name
Toxicol Mech Methodsjournal_title
Toxicology mechanisms and methodsauthors
Ekambaram P,Jayachandran T,Dhakshinamoorthy Ldoi
10.3109/15376516.2012.725432subject
Has Abstractpub_date
2013-02-01 00:00:00pages
113-9issue
2eissn
1537-6516issn
1537-6524journal_volume
23pub_type
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journal_title:Toxicology mechanisms and methods
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journal_title:Toxicology mechanisms and methods
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journal_title:Toxicology mechanisms and methods
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journal_title:Toxicology mechanisms and methods
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journal_title:Toxicology mechanisms and methods
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journal_title:Toxicology mechanisms and methods
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journal_title:Toxicology mechanisms and methods
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abstract::ABSTRACT Body packers are people who illegally carry drugs, mostly cocaine as well as opium and/or heroin, concealed within their bodies. The packets are inserted in the mouth, rectum, or vagina in order to get across borders without being detected. In this presentation we report a case of an opium body packer and rev...
journal_title:Toxicology mechanisms and methods
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journal_title:Toxicology mechanisms and methods
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journal_title:Toxicology mechanisms and methods
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journal_title:Toxicology mechanisms and methods
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journal_title:Toxicology mechanisms and methods
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journal_title:Toxicology mechanisms and methods
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journal_title:Toxicology mechanisms and methods
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abstract:UNLABELLED:Cisplatin (CP) is an antineoplastic agent that induces nephrotoxicity and oxidative stress. It is unknown whether renal tight junction (TJ) proteins expression and localization are modified in CP-induced nephrotoxicity. OBJECTIVE:To study if the expression of the TJ proteins occludin, claudin-2, claudin-5 a...
journal_title:Toxicology mechanisms and methods
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journal_title:Toxicology mechanisms and methods
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