Abstract:
:ABSTRACT Triptolide is a diterpene triepoxide isolated from the traditional Chinese medicinal vine Trypterygium wilfordii hook f. (T. wilfordii). It possesses multiple biological activities, such as antitumor, immunosuppression, and antifertility. Previous studies suggested that triptolide might be a potential candidate for post-testicular male contraceptive agent. Nevertheless, the mechanisms of triptolide-induced reproductive toxicity remain unclear. In the present study, the results of reverse-transcription PCR and Western blotting revealed that triptolide inhibited the expression of Cx43 compared with the different effect of estradiol in cultured SCs from male rats. Further computational study revealed that triptolide can bind to the active site of human estrogenic 17beta-hydroxysteroid dehydrogenase and human estrogen receptorbeta. Therefore, our results indicated that male reproductive toxicity induced by triptolide was associated with the effects on intratesticular estrogen levels and estrogen receptors rather than its cytotoxicity.
journal_name
Toxicol Mech Methodsjournal_title
Toxicology mechanisms and methodsauthors
Ni B,Zhu T,Jiang Z,Zhang R,Zhang T,Zhang Ldoi
10.1080/15376510802192882subject
Has Abstractpub_date
2008-01-01 00:00:00pages
717-24issue
9eissn
1537-6516issn
1537-6524journal_volume
18pub_type
杂志文章abstract::How triptolide is associated with mitochondrial dysfunction and apoptosis in connection with its hepatotoxicity remains unclear. The objective of our study was to find out the link between mitochondrial dynamics and cell death in triptolide induced hepatotoxicity. We treated L02 cells with 25 nM concentration of tript...
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