Abstract:
:The objective of this study was to examine the toxicity of 1-(4-morpholinophenyl)-3-(4-fluorophenyl)-propenone (A) and 1-(4-morpholinophenyl)-3-(3-fluorophenyl)-propenone (B) on Spodoptera frugiperda (Sf9) cells and the mechanism of the toxicity. By cell-based thiazolyl blue tetrazolium bromide (MTT) assay, we found that the IC(50) were 35.45 μM for A and 31.97 μM for B respectively. Formation of apoptotic bodies was observed at 24 h in the treated cells. There was a significant increase of DNA ladders in the treated Sf9 cells compared to controls. In the presence of 50 μM compound A and B for 24 h, ATP content of Sf9 cells reduced by approximately 50%. Compared to the controls, the significant over-expression of caspase-3 in treated cells was measured by caspase-3 activity kit, and the loss of mitochondrial membrane potential (ΔΨm) was detected in apoptosis cells. The results suggested that the two compounds could be identified as new potent cell apoptosis inducers.
journal_name
Toxicol Mech Methodsjournal_title
Toxicology mechanisms and methodsauthors
Zhang X,Tao K,Hou Tdoi
10.3109/15376516.2012.658979subject
Has Abstractpub_date
2012-05-01 00:00:00pages
315-20issue
4eissn
1537-6516issn
1537-6524journal_volume
22pub_type
杂志文章abstract::Renal disease is a common complication of diabetes mellitus. The pathogenesis of diabetic nephropathy is not well understood, but hyperglycemia seems to be a crucial factor. Recent evidence indicates that the overproduction of reactive oxygen species, observed in both clinical and experimental diabetes, and mitochondr...
journal_title:Toxicology mechanisms and methods
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