Abstract:
:Color and geometric characteristics of stained areas in histochemical slides are among the features pathologists assess to evaluate the severity of lesions. In this research, image processing techniques were used to perform objective quantification of these characteristics in images of H&E-stained spleen tissues. A segmentation algorithm was developed to isolate the areas of interest in microscopic tissue images. Image features important to pathological evaluation were then extracted. These features were used to build statistical and neural network models to predict pathologist scores. A linear regression model predicted the scores to an R(2)-value of 0.6, and a neural network model classified samples to an accuracy of 75%. The results show the usefulness of image processing as a tool for pathological evaluation.
journal_name
Toxicol Mech Methodsjournal_title
Toxicology mechanisms and methodsauthors
Liu X,Tan J,Hatem I,Smith BLdoi
10.1080/15376520490434638subject
Has Abstractpub_date
2004-01-01 00:00:00pages
301-7issue
5eissn
1537-6516issn
1537-6524journal_volume
14pub_type
杂志文章abstract::For good performance in clinical and forensic toxicology, it is important to be aware of the signs and symptoms related to xenobiotic exposure since they will assist clinicians to reach a useful and rapid diagnosis. This manuscript highlights and critically analyses clinical and forensic imaging related to ethanol abu...
journal_title:Toxicology mechanisms and methods
pub_type: 杂志文章,评审
doi:10.3109/15376516.2013.869782
更新日期:2014-02-01 00:00:00
abstract::Human exposure to copper oxide (CuO) nanoparticles (NPs) is rapidly increasing and for this reason reliable toxicity test systems are urgently needed. Recently, the acute cytotoxicity of CuO NPs using the new toxicity test based on human bone marrow mesenchymal stem cells (hBMMSCs) has been evaluated. It was shown tha...
journal_title:Toxicology mechanisms and methods
pub_type: 杂志文章
doi:10.1080/15376516.2016.1220654
更新日期:2016-10-01 00:00:00
abstract::The present study was designed to investigate the effects of aqueous Azadirchta Indica leaf extract (AAILE) on serum glycoprotein contents and tumor incidence rate in colon of rats subjected to Dimethylhydrazine (DMH) treatment. Forty rats were divided equally and randomly into four groups viz., Group I (normal contro...
journal_title:Toxicology mechanisms and methods
pub_type: 杂志文章
doi:10.1080/15376510802646523
更新日期:2009-05-01 00:00:00
abstract::Owing to their structure and function, low-density lipoproteins (LDLs) are particularly susceptible to the oxidative modifications. To prevent against oxidative modification of LDL, L-carnitine, with endogenous small water-soluble quaternary amine possessing antioxidative properties, was used. The aim of this paper wa...
journal_title:Toxicology mechanisms and methods
pub_type: 杂志文章
doi:10.1080/15376510701623508
更新日期:2008-07-01 00:00:00
abstract::Angiogenesis, disturbance in redox homeostasis, and deregulated redox signaling are considered as common hallmarks of cancer progression and chemo resistance. In this context, PERK (protein kinase PKR-like ER kinase) branch of the unfolded protein response (UPR), an adaptive mechanism triggered by endoplasmic reticulu...
journal_title:Toxicology mechanisms and methods
pub_type: 杂志文章
doi:10.1080/15376516.2020.1727596
更新日期:2020-05-01 00:00:00
abstract::The brain is a well-organized, yet highly complex, organ in the mammalian system. Most investigators use the whole brain, instead of a selected brain region(s), for biochemical analytes as toxicological endpoints. As a result, the obtained data is often of limited value, since their significance is compromised due to ...
journal_title:Toxicology mechanisms and methods
pub_type: 杂志文章
doi:10.1080/15376520490429175
更新日期:2004-01-01 00:00:00
abstract::This article described an improved brine shrimp larvae lethality microwell test method. A simply designed connecting vessel with alternative photoperiod was used to culture and collect high yield of active Artemia parthenogenetica nauplii for brine shrimp larvae lethality microwell test. Using this method, pure A. par...
journal_title:Toxicology mechanisms and methods
pub_type: 杂志文章
doi:10.3109/15376516.2011.583297
更新日期:2012-01-01 00:00:00
abstract::In the present investigation acetylcholinesterase (AChE) was estimated in erythrocytes from blood samples exposed to four commonly used organophosphorus pesticides in vitro. The aim of the study was to determine the IC50 concentration of the pesticides monocrotophos, chlorpyrifos, profenofos, and acephate as inhibitor...
journal_title:Toxicology mechanisms and methods
pub_type: 杂志文章
doi:10.1080/15376520600719281
更新日期:2006-01-01 00:00:00
abstract::Preeclampsia is a multisystem disorder with profound implications on both mother and fetus. Analysis of umbilical cord blood red blood cell (RBC) changes shall depict the fetal response to pregnancy-specific complications like preeclampsia. This study aims to analyze the regulation relationship between HSP90α and heme...
journal_title:Toxicology mechanisms and methods
pub_type: 杂志文章
doi:10.3109/15376516.2012.725432
更新日期:2013-02-01 00:00:00
abstract:CONTEXT:The critical role of CaMKIIδ isoforms in cardiac hypertrophy is well documented. OBJECTIVE:This study was aimed to investigate the possible inhibitory effects of aliskiren (ALS) and/or carvedilol (CAV) on CaMKIIδ isoforms expression in experimental cardiac hypertrophy. MATERIALS AND METHODS:Male Wistar albino...
journal_title:Toxicology mechanisms and methods
pub_type: 杂志文章
doi:10.3109/15376516.2015.1128035
更新日期:2016-02-01 00:00:00
abstract::Limited information is available regarding systemic changes in mammals associated with exposures to petroleum/hydrocarbon fuels. In this study, systemic toxicity of JP-8 jet fuel was observed in a rat inhalation model at different JP-8 fuel vapor concentrations (250, 500, or 1000 mg/m(3), for 91 days). Gel electrophor...
journal_title:Toxicology mechanisms and methods
pub_type: 杂志文章
doi:10.3109/15376511003681009
更新日期:2010-05-01 00:00:00
abstract::The objective of this study was to examine the toxicity of 1-(4-morpholinophenyl)-3-(4-fluorophenyl)-propenone (A) and 1-(4-morpholinophenyl)-3-(3-fluorophenyl)-propenone (B) on Spodoptera frugiperda (Sf9) cells and the mechanism of the toxicity. By cell-based thiazolyl blue tetrazolium bromide (MTT) assay, we found t...
journal_title:Toxicology mechanisms and methods
pub_type: 杂志文章
doi:10.3109/15376516.2012.658979
更新日期:2012-05-01 00:00:00
abstract::ABSTRACT Male Wistar rats with streptozotocin-induced diabetes received a control diet and a pyridoxine-deficient diet. The animals were divided at random into four groups: control rats (CR), control diabetic rats (CDR), diabetic rats receiving a pyridoxine-free diet (DRB6), and diabetic rats receiving saline solution...
journal_title:Toxicology mechanisms and methods
pub_type: 杂志文章
doi:10.1080/15376510601003744
更新日期:2007-01-01 00:00:00
abstract::Histone deacetylase 2 (HDAC2), a prominent member of the class I HDAC family, plays crucial roles in inflammation and other pathological processes. Recent studies have found that the activity and expression of HDAC2 were altered under oxidative stress conditions. The aim of the current study was to elucidate the expre...
journal_title:Toxicology mechanisms and methods
pub_type: 杂志文章
doi:10.3109/15376516.2012.716091
更新日期:2012-10-01 00:00:00
abstract::The current study evaluated in vitro and in vivo toxicity of carboxyl or amine polyethylene glycol (PEG) surface functionalization of single-walled carbon nanotubes (SWCNTs). Assessments of cytotoxicity, genotoxicity, immunotoxicity, and oxidative stress were performed in vitro and in vivo (in a 1-month follow-up stud...
journal_title:Toxicology mechanisms and methods
pub_type: 杂志文章
doi:10.1080/15376516.2018.1540674
更新日期:2019-05-01 00:00:00
abstract::The specific properties of silver nanoparticles (AgNPs), such as antimicrobial activity and electrical conductivity, allow them to be used in many fields. However, their expanding application is also raising health, environmental and safety concerns. Previous in vivo AgNP toxicity studies have indicated a gender-diffe...
journal_title:Toxicology mechanisms and methods
pub_type: 杂志文章
doi:10.3109/15376516.2013.780196
更新日期:2013-07-01 00:00:00
abstract::ABSTRACT Although copper is an essential metal and cadmium is an environmental pollutant, both are toxic when present in excess. Metallothionein and glutathione are two of the key components that participate in detoxification of copper and cadmium. In the present study the role of glutathione in resistance to copper a...
journal_title:Toxicology mechanisms and methods
pub_type: 杂志文章
doi:10.1080/15376510601091392
更新日期:2007-01-01 00:00:00
abstract:BACKGROUND:Arsenic has been shown to cause various diseases (such as blackfoot disease, cardiovascular diseases, bladder cancer and skin cancer) in many areas of the world. However, the effects of arsenic on cardiac rhythm functions still lack investigation. METHODS:In this study, different concentrations of arsenic w...
journal_title:Toxicology mechanisms and methods
pub_type: 杂志文章
doi:10.1080/15376516.2018.1440679
更新日期:2018-07-01 00:00:00
abstract::The β-glucans (β-G) are polysaccharides produced by various organisms, and sulfation of β-G renders them more soluble. With the objective to assess the effects of sulfated and non-sulfated β-G extracted from Agaricus brasiliensis in MCF-7 cells, assays were used to evaluate cytotoxicity, genotoxicity, cell proliferati...
journal_title:Toxicology mechanisms and methods
pub_type: 杂志文章
doi:10.3109/15376516.2015.1043762
更新日期:2015-01-01 00:00:00
abstract::Valproic acid (VPA), an anticonvulsant and mood-stabilizing drug, is widely used for the treatment of different types of seizures and myoclonic epilepsy. Several mechanisms have been suggested for VPA hepatotoxicity, and most of them are associated with oxidative stress. It seems that oxidative stress by VPA treatment...
journal_title:Toxicology mechanisms and methods
pub_type: 杂志文章
doi:10.3109/15376516.2013.821567
更新日期:2013-10-01 00:00:00
abstract:BACKGROUND AND OBJECTIVES:The aim of this study was to evaluate genotoxicity and mutagenicity in peripheral blood and buccal mucosal cells in mucopolysaccharidosis (MPS) I, II or VI patients. METHODS:A total of 12 patients with MPS type I, II and VI attended at the Institute of Genetics and Inborn Errors of Metabolism...
journal_title:Toxicology mechanisms and methods
pub_type: 杂志文章
doi:10.3109/15376516.2014.956913
更新日期:2014-12-01 00:00:00
abstract::How triptolide is associated with mitochondrial dysfunction and apoptosis in connection with its hepatotoxicity remains unclear. The objective of our study was to find out the link between mitochondrial dynamics and cell death in triptolide induced hepatotoxicity. We treated L02 cells with 25 nM concentration of tript...
journal_title:Toxicology mechanisms and methods
pub_type: 杂志文章
doi:10.1080/15376516.2019.1669247
更新日期:2020-02-01 00:00:00
abstract:OBJECTIVE:The objective of the present study is to evaluate the antihyperlipidemic effect of Chlorella pyrenoidosa in diabetic rats exposed to cadmium (Cd). MATERIALS AND METHODS:Group 1 and 2 rats were treated as control and C. pyrenoidosa control. Group 3 and 4 rats were given single injection of streptozotocin (40 ...
journal_title:Toxicology mechanisms and methods
pub_type: 杂志文章
doi:10.3109/15376516.2012.714007
更新日期:2012-10-01 00:00:00
abstract::In this study, 162 samples were analysed for monomer styrene content with using high performance liquid chromatography (HPLC) method in hot tea, milk, cocoa milk. The monomer styrene content, expressed in microg/l of drink and the level of migration of styrene monomer were varied from 0.61 to 8.15 for hot tea, from 0....
journal_title:Toxicology mechanisms and methods
pub_type: 杂志文章
doi:10.1080/15376510802510299
更新日期:2009-03-01 00:00:00
abstract::Mitochondrial haplogroups can differ in frequency of occurrence in those who are 100 years old and in the rest of the population. These differences are not universally observed; moreover, they are population-specific. We analyzed the haplogroups in 97 Polish 100-year-olds and compared them with those of more than 145 ...
journal_title:Toxicology mechanisms and methods
pub_type: 杂志文章
doi:10.1080/15376520490257572
更新日期:2004-01-01 00:00:00
abstract::Benzene (C6H6) is one of the most commonly used industrial chemicals causing environmental pollution. This study aimed to examine the effect of benzene and its metabolite hydroquinone on glucose regulating organs, liver and pancreas, and to reveal the involved toxic mechanisms, in rats. In the in vivo part, benzene wa...
journal_title:Toxicology mechanisms and methods
pub_type: 杂志文章
doi:10.3109/15376516.2015.1053650
更新日期:2015-01-01 00:00:00
abstract::Up to now, the ability of target cells to activate protein kinase C (PKC) and protein kinase D (PKD) (which is often a downstream target of PKC) has not been examined in natural killer (NK) lymphocytes. Here we examined whether exposure of human NK cells to lysis sensitive tumor cells activated PKC and PKD. The result...
journal_title:Toxicology mechanisms and methods
pub_type: 杂志文章
doi:10.3109/15376516.2015.1070226
更新日期:2015-01-01 00:00:00
abstract::The US military has placed major emphasis on developing therapeutics against nerve agents (NA). Current efforts are hindered by the lack of effective in vitro cellular models to aid in the preliminary screening of potential candidate drugs/antidotes. The development of an in vitro cellular model to aid in discovering ...
journal_title:Toxicology mechanisms and methods
pub_type: 杂志文章
doi:10.3109/15376516.2014.943442
更新日期:2014-10-01 00:00:00
abstract::In this study, the preventive effects of NGF against colistin-induced autophagy and apoptosis in PC12 cells have been investigated. Fluorescence microscopy, real-time PCR, transmission electron microscopy (TEM), flow cytometery, and western blotting technique were used. The results showed that large amounts of autopha...
journal_title:Toxicology mechanisms and methods
pub_type: 杂志文章
doi:10.1080/15376516.2018.1534298
更新日期:2019-03-01 00:00:00
abstract:BACKGROUND:Oxytocin (OT) injections have been indiscriminately used to milk cattle in dairy industries. There is no study available regarding surveillance of OT in market milk samples. MATERIAL AND METHODS:OT from milk samples was extracted by precipitation with trichloroacetic acid and passed through the solid phase ...
journal_title:Toxicology mechanisms and methods
pub_type: 杂志文章
doi:10.3109/15376516.2014.910851
更新日期:2014-06-01 00:00:00