Abstract:
:The influence of experimental conditions on gradient high-performance liquid chromatography/diode array detector separation and identification of 13 psychotropic drugs belonging to two groups--phenothiazines and tricyclic antidepressants--was examined. The main interaction effects of three experimental factors were determined according to a 2 3 factorial design, and the optimum conditions of analysis were searched for. The degree to which the chromatographic peaks overlap is taken into account in a proposed criterion for separation quality. The screening analysis proposed was tested on whole blood samples spiked with mixtures of the examined drugs. The method was characterized by such validation parameters as relative retention times, absorbance ratios at two wavelengths, detection limits, linearity ranges, and extraction recoveries. The method proved to be a suitable tool for the identification of psychotropic drugs tested for forensic purposes.
journal_name
Toxicol Mech Methodsjournal_title
Toxicology mechanisms and methodsauthors
Madej K,Parczewski A,Kała Mdoi
10.1080/15376510309842subject
Has Abstractpub_date
2003-01-01 00:00:00pages
121-7issue
2eissn
1537-6516issn
1537-6524journal_volume
13pub_type
杂志文章abstract::Miniscrew implants are widely used nowadays in orthodontic treatments due to their good results in clinical practice. However, data regarding the biocompatibility of commercially available orthodontic miniscrews and temporary devices are very scarce, and their role as genotoxicity inducers has been not previously eval...
journal_title:Toxicology mechanisms and methods
pub_type: 杂志文章
doi:10.3109/15376516.2015.1053652
更新日期:2015-01-01 00:00:00
abstract::In this study, the preventive effects of NGF against colistin-induced autophagy and apoptosis in PC12 cells have been investigated. Fluorescence microscopy, real-time PCR, transmission electron microscopy (TEM), flow cytometery, and western blotting technique were used. The results showed that large amounts of autopha...
journal_title:Toxicology mechanisms and methods
pub_type: 杂志文章
doi:10.1080/15376516.2018.1534298
更新日期:2019-03-01 00:00:00
abstract::Impurities in drug substances and drug products generally do not have beneficial effects and may impose a risk without associated benefit. Levofloxacin n-oxide is an impurity isolated from levofloxacin. However there is insufficient toxic information about levofloxacin n-oxide. This study investigates the genotoxicity...
journal_title:Toxicology mechanisms and methods
pub_type: 杂志文章
doi:10.3109/15376516.2011.635319
更新日期:2012-04-01 00:00:00
abstract::Oxidative stress in biological systems can result in radical-induced lipid peroxidation (LPO), which can lead to the production of secondary reactive by-products such as 4-hydroxy-2-nonenal (HNE), malondialdehyde (MDA), acrolein, and acetaldehyde. These deleterious compounds are known to react with and concomitantly m...
journal_title:Toxicology mechanisms and methods
pub_type: 杂志文章
doi:10.1080/15376510903104224
更新日期:2009-09-01 00:00:00
abstract::In this study, 162 samples were analysed for monomer styrene content with using high performance liquid chromatography (HPLC) method in hot tea, milk, cocoa milk. The monomer styrene content, expressed in microg/l of drink and the level of migration of styrene monomer were varied from 0.61 to 8.15 for hot tea, from 0....
journal_title:Toxicology mechanisms and methods
pub_type: 杂志文章
doi:10.1080/15376510802510299
更新日期:2009-03-01 00:00:00
abstract::Methylglyoxal (MG) is a reactive dicarbonyl metabolite originated mainly from glucose degradation pathway that plays an important role in the pathogenesis of diabetes mellitus (DM). Reactions of MG with biological macromolecules (proteins, DNA and lipids) can induce cytotoxicity and apoptosis. Here, human erythrocytes...
journal_title:Toxicology mechanisms and methods
pub_type: 杂志文章
doi:10.1080/15376516.2017.1285971
更新日期:2017-05-01 00:00:00
abstract:AIM:Nicotine exerts a number of physiological effects. The purpose of this study was to determine the effects of nicotine on thioacetamide (TAA)-induced liver fibrosis in mice. MATERIALS AND METHODS:For in vivo experiments, hepatic fibrosis was induced by TAA (0.25 g/kg, i.p.) three times a week for 6 weeks. Mice of T...
journal_title:Toxicology mechanisms and methods
pub_type: 杂志文章
doi:10.1080/15376516.2017.1323256
更新日期:2017-09-01 00:00:00
abstract::In cosmetics and food products, parabens are widely used as antimicrobial agents. Reports have suggested that parabens may be linked to infertility, owing to their effects on basal steroidogenesis properties or their capacity to inflict mitochondrial damage. Despite growing concerns about parabens as endocrine disrupt...
journal_title:Toxicology mechanisms and methods
pub_type: 杂志文章
doi:10.1080/15376516.2020.1741052
更新日期:2020-06-01 00:00:00
abstract::Toxicogenomics has evolved into a useful technique for providing greater mechanistic insights into adverse effects that will spur the development of novel approaches for identifying and understanding toxicity issues. The ability to capture a snapshot of the transcriptome at any given time during the development of an ...
journal_title:Toxicology mechanisms and methods
pub_type: 杂志文章
doi:10.1080/15376520600558317
更新日期:2006-01-01 00:00:00
abstract::Nematode Caenorhabditis elegans (C. elegans) was used to investigate the impact of silver nanoparticles (SNP), multiwalled carbon nanotubes (MWCNT), and polyamidoamine dendrimers (PAMAM) used in concentration of 1010 particle/mL. Population-based observations and gene expression analysis were employed in this study. S...
journal_title:Toxicology mechanisms and methods
pub_type: 杂志文章
doi:10.1080/15376516.2018.1449277
更新日期:2018-07-01 00:00:00
abstract::Owing to their structure and function, low-density lipoproteins (LDLs) are particularly susceptible to the oxidative modifications. To prevent against oxidative modification of LDL, L-carnitine, with endogenous small water-soluble quaternary amine possessing antioxidative properties, was used. The aim of this paper wa...
journal_title:Toxicology mechanisms and methods
pub_type: 杂志文章
doi:10.1080/15376510701623508
更新日期:2008-07-01 00:00:00
abstract::ABSTRACT When two toxicants overtly producing similar effects are given together, the effect of the combination may either be equal to the algebraic sum of their individual effects or may be more than or less than the algebraic sum of their individual effects. Therefore, to ascertain the nature of interaction precisel...
journal_title:Toxicology mechanisms and methods
pub_type: 杂志文章
doi:10.1080/15376510601177654
更新日期:2007-01-01 00:00:00
abstract::Cadmium is one of the most toxic elements to which man can be exposed at work or in the environment. By far, the most salient toxicological property of Cd is its exceptionally long half-life in the human body. Once absorbed, Cd accumulates in the human body, particularly in the liver and other vital organs. The cellul...
journal_title:Toxicology mechanisms and methods
pub_type: 杂志文章
doi:10.3109/15376510903325670
更新日期:2009-10-01 00:00:00
abstract::Concern over the influence of carbon nanotubes (CNTs) on human health has arisen due to advances; however, little is known about the potential toxicity of CNTs. In this study, impurity-free single-wall carbon nanotubes (SWCNTs), with different physical properties in cell culture medium, were prepared by a novel disper...
journal_title:Toxicology mechanisms and methods
pub_type: 杂志文章
doi:10.3109/15376516.2013.811568
更新日期:2013-10-01 00:00:00
abstract::Ethanol metabolism is accompanied by generation of free radicals that damage cell components, especially lipids. The present study was designed to investigate the efficacy of the preventive effect of black tea on the lipid oxidative modifications in different tissues (plasma, liver, brain, kidney, stomach, lung, intes...
journal_title:Toxicology mechanisms and methods
pub_type: 杂志文章
doi:10.1080/15376510701624050
更新日期:2008-07-01 00:00:00
abstract::To explore the toxic effect of T-2 toxin on mouse Leydig cells and its underlying molecular mechanisms, we isolated Leydig cells from mature mice, set-up Leydig cells culture, treated cells with T-2 toxin, evaluated cell proliferation, detected the caspase-3 activity, mitochondrial activity and apoptosis rate, and mea...
journal_title:Toxicology mechanisms and methods
pub_type: 杂志文章
doi:10.1080/15376516.2017.1354413
更新日期:2018-01-01 00:00:00
abstract::The current study evaluated in vitro and in vivo toxicity of carboxyl or amine polyethylene glycol (PEG) surface functionalization of single-walled carbon nanotubes (SWCNTs). Assessments of cytotoxicity, genotoxicity, immunotoxicity, and oxidative stress were performed in vitro and in vivo (in a 1-month follow-up stud...
journal_title:Toxicology mechanisms and methods
pub_type: 杂志文章
doi:10.1080/15376516.2018.1540674
更新日期:2019-05-01 00:00:00
abstract::The B6C3F1 mouse is the standard mouse strain used in National Toxicology Program (NTP) carcinogenesis studies. Over time, increased liver tumorigenesis that was correlated with elevated body weights was noted in males and females. NTP therefore replaced the NIH-07 diet with the NTP-2000 diet and returned to group hou...
journal_title:Toxicology mechanisms and methods
pub_type: 杂志文章
doi:10.3109/15376516.2012.672482
更新日期:2012-07-01 00:00:00
abstract::Due to their exceptional properties, gold nanoparticles (AuNPs) have shown promising medical and technological applications in the treatment of cancer and the development of antimicrobial packaging and time-temperature indicators in the food sector. However, little is known about their cytotoxicity when they come into...
journal_title:Toxicology mechanisms and methods
pub_type: 杂志文章
doi:10.3109/15376516.2013.869783
更新日期:2014-03-01 00:00:00
abstract::Carbon tetrachloride (CCl4) is a highly toxic industrial solvent with pronounced systemic toxicity including brain. Neurotoxicity may be a direct result of hepatic dysfunction from CCl4 intoxication. Over the years CCl4 has been used as an excellent model for studying experimentally induced neurotoxicity in murine mod...
journal_title:Toxicology mechanisms and methods
pub_type: 杂志文章
doi:10.3109/15376516.2014.945108
更新日期:2014-10-01 00:00:00
abstract::Misuse of various chemicals, such as chemical warfare agents, industrial chemicals or pesticides during warfare or terrorists attacks requires adequate protection. Thus, development and evaluation of novel decontamination dispositives and techniques are needed. In this study, in vitro permeation and decontamination of...
journal_title:Toxicology mechanisms and methods
pub_type: 杂志文章
doi:10.3109/15376516.2012.686535
更新日期:2012-09-01 00:00:00
abstract::Tumor progression is the continual selection of variant subpopulations of malignant cells that have acquired increasing levels of genetic instability (Nowell Science 1976, 194, 23-28). This instability is manifested as chromosomal aneuploidy or translocations, viral integration or somatic mutations that typically affe...
journal_title:Toxicology mechanisms and methods
pub_type: 杂志文章,评审
doi:10.3109/15376516.2011.562759
更新日期:2011-05-01 00:00:00
abstract::ABSTRACT The ideal toxicity biomarker is composed of the properties of prediction (is detected prior to traditional pathological signs of injury), accuracy (high sensitivity and specificity), and mechanistic relationships to the endpoint measured (biological relevance). Gene expression-based toxicity biomarkers ("sign...
journal_title:Toxicology mechanisms and methods
pub_type: 杂志文章
doi:10.1080/15376510701857072
更新日期:2008-01-01 00:00:00
abstract::The US military has placed major emphasis on developing therapeutics against nerve agents (NA). Current efforts are hindered by the lack of effective in vitro cellular models to aid in the preliminary screening of potential candidate drugs/antidotes. The development of an in vitro cellular model to aid in discovering ...
journal_title:Toxicology mechanisms and methods
pub_type: 杂志文章
doi:10.3109/15376516.2014.943442
更新日期:2014-10-01 00:00:00
abstract::This study applied broccoli and cauliflower extracts (whole, floret, and stem) to zebrafish larvae in parallel to receive 100 mJ/cm(2) of UVB six times, and recorded their fin malformation phenotypes. Chemopreventive effects of each group, including UVB, whole-, floret-, and stem-extracts of broccoli and cauliflower o...
journal_title:Toxicology mechanisms and methods
pub_type: 杂志文章
doi:10.3109/15376516.2010.529191
更新日期:2011-01-01 00:00:00
abstract::The need for reliable results in Toxicological Analysis is recognized and required worldwide. The analytical validation ensures that a method will provide trustworthy information about a particular sample when applied in accordance with a predefined protocol, being able to determine a specific analyte at a distinct co...
journal_title:Toxicology mechanisms and methods
pub_type: 杂志文章
doi:10.1080/15376516.2020.1839612
更新日期:2021-01-01 00:00:00
abstract::Histone deacetylase 2 (HDAC2), a prominent member of the class I HDAC family, plays crucial roles in inflammation and other pathological processes. Recent studies have found that the activity and expression of HDAC2 were altered under oxidative stress conditions. The aim of the current study was to elucidate the expre...
journal_title:Toxicology mechanisms and methods
pub_type: 杂志文章
doi:10.3109/15376516.2012.716091
更新日期:2012-10-01 00:00:00
abstract::Preeclampsia is a multisystem disorder with profound implications on both mother and fetus. Analysis of umbilical cord blood red blood cell (RBC) changes shall depict the fetal response to pregnancy-specific complications like preeclampsia. This study aims to analyze the regulation relationship between HSP90α and heme...
journal_title:Toxicology mechanisms and methods
pub_type: 杂志文章
doi:10.3109/15376516.2012.725432
更新日期:2013-02-01 00:00:00
abstract::Mitochondrial haplogroups can differ in frequency of occurrence in those who are 100 years old and in the rest of the population. These differences are not universally observed; moreover, they are population-specific. We analyzed the haplogroups in 97 Polish 100-year-olds and compared them with those of more than 145 ...
journal_title:Toxicology mechanisms and methods
pub_type: 杂志文章
doi:10.1080/15376520490257572
更新日期:2004-01-01 00:00:00
abstract::This study was to evaluate the protective effects of Danshensu on liver injury induced by omethoate in Sprague Dawley rats. The acute omethoate poisoning model was established by administrating subcutaneously with omethoate at a single dose of 60 mg/kg. Danshensu treatment markedly inhibited the increases of aspartate...
journal_title:Toxicology mechanisms and methods
pub_type: 杂志文章
doi:10.3109/15376516.2010.514963
更新日期:2010-10-01 00:00:00