Abstract:
:Methylglyoxal (MG) is a reactive dicarbonyl metabolite originated mainly from glucose degradation pathway that plays an important role in the pathogenesis of diabetes mellitus (DM). Reactions of MG with biological macromolecules (proteins, DNA and lipids) can induce cytotoxicity and apoptosis. Here, human erythrocytes, leukocytes and platelets were acutely exposed to MG at concentration ranging from 0.025 to 10 mM. Afterwards, hemolysis and osmotic fragility in erythrocytes, DNA damage and cell viability in leukocytes, and the activity of purinergic ecto-nucleotidases in platelets were evaluated. The levels of glycated products from leukocytes and free amino groups from erythrocytes and platelets were also measured. MG caused fragility of membrane, hemolysis and depletion of amino groups in erythrocytes. DNA damage, loss of cell viability and increased levels of glycated products were observed in leukocytes. In platelets, MG inhibited the activity of enzymes NTPDase, 5'-nucleotidase and adenosine deaminase (ADA) without affecting the levels of free amino groups. Our findings provide insights for understanding the mechanisms involved in MG acute toxicity towards distinct blood cells.
journal_name
Toxicol Mech Methodsjournal_title
Toxicology mechanisms and methodsauthors
Prestes AS,Dos Santos MM,Ecker A,Zanini D,Schetinger MR,Rosemberg DB,da Rocha JB,Barbosa NVdoi
10.1080/15376516.2017.1285971subject
Has Abstractpub_date
2017-05-01 00:00:00pages
307-317issue
4eissn
1537-6516issn
1537-6524journal_volume
27pub_type
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