Abstract:
:Neutrophil extracellular traps (NETs) are released as neutrophils die in vitro in a process requiring hours, leaving a temporal gap that invasive microbes may exploit. Neutrophils capable of migration and phagocytosis while undergoing NETosis have not been documented. During Gram-positive skin infections, we directly visualized live polymorphonuclear cells (PMNs) in vivo rapidly releasing NETs, which prevented systemic bacterial dissemination. NETosis occurred during crawling, thereby casting large areas of NETs. NET-releasing PMNs developed diffuse decondensed nuclei, ultimately becoming devoid of DNA. Cells with abnormal nuclei showed unusual crawling behavior highlighted by erratic pseudopods and hyperpolarization consistent with the nucleus being a fulcrum for crawling. A requirement for both Toll-like receptor 2 and complement-mediated opsonization tightly regulated NET release. Additionally, live human PMNs injected into mouse skin developed decondensed nuclei and formed NETS in vivo, and intact anuclear neutrophils were abundant in Gram-positive human abscesses. Therefore early in infection NETosis involves neutrophils that do not undergo lysis and retain the ability to multitask.
journal_name
Nat Medjournal_title
Nature medicineauthors
Yipp BG,Petri B,Salina D,Jenne CN,Scott BN,Zbytnuik LD,Pittman K,Asaduzzaman M,Wu K,Meijndert HC,Malawista SE,de Boisfleury Chevance A,Zhang K,Conly J,Kubes Pdoi
10.1038/nm.2847subject
Has Abstractpub_date
2012-09-01 00:00:00pages
1386-93issue
9eissn
1078-8956issn
1546-170Xpii
nm.2847journal_volume
18pub_type
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