Abstract:
:There is inconsistent use of Matrigel for experiments with the HTR8/SVneo first trimester trophoblast and other cell lines. We quantified the effects of Matrigel on the expression of genes considered to be markers of extravillous cytotrophoblast (EVT) differentiation and invasive potential. Culture on Matrigel promoted formation of "endothelial-like" tubes and reduced mRNA expression of matrix metalloproteinase 2 (MMP2), cytokeratin 7 (KRT7) and integrin alpha 1 (ITGA1), while increasing VE-cadherin (CDH5) expression consistent with a vascular phenotype. This process may constitute part of the endothelial cell mimicry exhibited by endovascular EVTs invading the maternal spiral arteries. HTR8/SVneo appears to be phenotypically polymorphic and adopt endovascular morphology on Matrigel.
journal_name
Placentajournal_title
Placentaauthors
Highet AR,Zhang VJ,Heinemann GK,Roberts CTdoi
10.1016/j.placenta.2012.04.003subject
Has Abstractpub_date
2012-07-01 00:00:00pages
586-8issue
7eissn
0143-4004issn
1532-3102pii
S0143-4004(12)00145-2journal_volume
33pub_type
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