Abstract：
:A psychrotolerant bacterium, strain T-3 (identified as Psychrobacter piscatorii), that exhibited an extraordinarily high catalase activity was isolated from the drain pool of a plant that uses H(2)O(2) as a bleaching agent. Its cell extract exhibited a catalase activity (19,700 U·mg protein(-1)) that was higher than that of Micrococcus luteus used for industrial catalase production. Catalase was approximately 10% of the total proteins in the cell extract of the strain. The catalase (PktA) was purified homogeneously by only two purification steps, anion exchange and hydrophobic chromatographies. The purified catalase exhibited higher catalytic efficiency and higher sensitivity of activity at high temperatures than M. luteus catalase. The deduced amino acid sequence showed the highest homology with catalase of Psycrobacter cryohalolentis, a psychrotolelant bacterium obtained from Siberian permafrost. These findings suggest that the characteristics of the PktA molecule reflected the taxonomic relationship of the isolate as well as the environmental conditions (low temperatures and high concentrations of H(2)O(2)) under which the bacterium survives. Strain T-3 efficiently produces a catalase (PktA) at a higher rate than Exiguobacterium oxidotolerans, which produces a very strong activity of catalase (EktA) at a moderate rate, in order to adapt to high concentration of H(2)O(2).
journal_name
Int J Mol Scijournal_title
International journal of molecular sciencesauthors
Kimoto H,Yoshimune K,Matsuyma H,Yumoto Idoi
10.3390/ijms13021733subject
Has Abstractpub_date
2012-01-01 00:00:00pages
1733-46issue
2issn
1422-0067pii
ijms-13-01733journal_volume
13pub_type
杂志文章abstract：:Central hypogonadism is a clinical condition, characterized by sexual symptoms and low serum testosterone levels, due to an impaired function of the hypothalamus or pituitary gland. Testosterone replacement therapy (TRT) is the standard treatment for hypogonadism, but it has some disadvantages. TRT is not a good optio...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms22010021
更新日期：2020-12-22 00:00:00
abstract：:Spirulina maxima, a microalga containing high levels of protein and many polyphenols, including chlorophyll a and C-phycocyanin, has antioxidant and anti-inflammatory therapeutic effects. However, the mechanisms where by Spirulina maxima ameliorates cognitive disorders induced by amyloid-β 1-42 (Aβ1-42) are not fully ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms18112401
更新日期：2017-11-13 00:00:00
abstract：:Recent reports highlight the potential tumorigenic role of Dentin Sialophosphoprotein (DSPP) and its cognate partner Matrix Metalloproteinase 20 (MMP-20) in Oral Squamous Cell Carcinomas (OSCCs). However, the function/mechanism of these roles is yet to be fully established. The present study aimed to investigate the e...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21134734
更新日期：2020-07-02 00:00:00
abstract：:Brassica campestris L., a hyperaccumulator of cadmium (Cd), is considered a candidate plant for efficient phytoremediation. The hairy roots of Brassica campestris L are chosen here as a model plant system to investigate the response mechanism of Brassica campestris L. to Cd stress. High-throughput sequencing technolog...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21010180
更新日期：2019-12-26 00:00:00
abstract：:Winter turnip rape (Brassica rapa L.) is a large-scale winter-only oil crop cultivated in Northwest China. However, its cold-resistant molecular mechanism remains inadequate. Studying the cold adaptation mechanisms of winter turnip rape based on the proteomic technique of isobaric tags for relative and absolute quanti...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms19113346
更新日期：2018-10-26 00:00:00
abstract：:The cluster Re6Se8I63- has been shown to induce preferential cell death of a hepatic carcinoma cell line, thus becoming a promising anti-cancer drug. Whether this cluster induces acute hemolysis or if it interacts with albumin remains unclear. The effect of acute exposure of human red blood cells to different concentr...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms16011728
更新日期：2015-01-13 00:00:00
abstract：:Melatonin is an indoleamine produced from the amino acid l-tryptophan, whereas metabolites of melatonin are known as kynuramines. One of the best-known kynuramines is N¹-acetyl-N¹-formyl-5-methoxykynuramine (AFMK). Melatonin has attracted scientific attention as a potent antioxidant and protector of tissue against oxi...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms18051014
更新日期：2017-05-08 00:00:00
abstract：:The mitochondrial genome of the nematode Romanomermis culicivorax encodes for miniaturized hairpin-like tRNA molecules that lack D- as well as T-arms, strongly deviating from the consensus cloverleaf. The single tRNA nucleotidyltransferase of this organism is fully active on armless tRNAs, while the human counterpart ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21239047
更新日期：2020-11-28 00:00:00
abstract：:Hypertrophic scars and keloids are fibroproliferative disorders that may arise after any deep cutaneous injury caused by trauma, burns, surgery, etc. Hypertrophic scars and keloids are cosmetically problematic, and in combination with functional problems such as contractures and subjective symptoms including pruritus,...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,meta分析,评审
doi：10.3390/ijms19030711
更新日期：2018-03-02 00:00:00
abstract：:Defensins are small and rather ubiquitous cysteine-rich anti-microbial peptides. These proteins may act against pathogenic microorganisms either directly (by binding and disrupting membranes) or indirectly (as signaling molecules that participate in the organization of the cellular defense). Even though defensins are ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21249380
更新日期：2020-12-09 00:00:00
abstract：:Previously, we demonstrated that the immediate administration of multitarget anxiolytic afobazole slows down the progression of neuronal damage in a 6-hydroxidodamine (6-OHDA) model of Parkinson's disease due to the activation of chaperone Sigma1R. The aim of the present study is to evaluate the therapeutic potential ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21207620
更新日期：2020-10-15 00:00:00
abstract：:The whitefly Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) causes serious crop losses worldwide by transmitting viruses. We have previously shown that salicylic acid (SA)-related plant defenses directly affect whiteflies. In this study, we applied exogenous SA to tomato plants in order to investigate the interac...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms17071048
更新日期：2016-06-30 00:00:00
abstract：:High-density lipoproteins (HDL) are known to have vasoprotective functions in peripheral arteries and many of these functions extend to brain-derived endothelial cells. Importantly, several novel brain-relevant HDL functions have been discovered using brain endothelial cells and in 3D bioengineered human arteries. The...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20030462
更新日期：2019-01-22 00:00:00
abstract：:The genus of Termitomyces purchased from the market has been identified as Termitomyces eurrhizus using the Internal Transcribed Spacer (ITS) method. An α-galactosidase from T. eurrhizus (TEG), a monomeric protein with a molecular mass of 72 kDa, was purified 146 fold by employing ion exchange chromatography and gel f...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms161226159
更新日期：2015-12-08 00:00:00
abstract：:The progression of cancer is associated with increases in amino acid uptake by cancer cells. Upon their entry into cells through specific transporters, exogenous amino acids are used to synthesize proteins, nucleic acids and lipids and to generate ATP. The essential amino acid leucine is also important for maintaining...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms19082373
更新日期：2018-08-12 00:00:00
abstract：BACKGROUND:P1G10 is a cysteine proteolytic fraction from Vasconcellea cundinamarcensis latex, obtained by chromatographic separation on Sephadex-G10 and ultrafiltration. This fraction enhances healing in different models of skin lesions, and displays a protective/healing effect against gastric ulcers, where it was sugg...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20184373
更新日期：2019-09-06 00:00:00
abstract：:Non-alcoholic fatty liver disease (NAFLD) is the most common liver disorder worldwide. Several lines of evidence have indicated a pathogenic role of insulin resistance, and a strong association with type 2 diabetes (T2MD) and metabolic syndrome. Importantly, NAFLD appears to enhance the risk for T2MD, as well as worse...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms141122933
更新日期：2013-11-20 00:00:00
abstract：:Bradyrhizobium japonicum is a nitrogen-fixing symbiont of soybean. In previous studies, transcriptomic profiling of B. japonicum USDA110, grown under various environmental conditions, revealed the highly induced gene aceA, encoding isocitrate lyase (ICL). The ICL catalyzes the conversion of isocitrate to succinate and...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms160716695
更新日期：2015-07-22 00:00:00
abstract：BACKGROUND:Left atrial appendage closure (LAAC) represents the interventional alternative to oral anticoagulation for stroke prevention in atrial fibrillation (AF). The metabolism of acylcarnitines was shown to affect cardiovascular diseases. This study evaluates the influence of successful LAAC on the metabolism of ac...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms19020500
更新日期：2018-02-07 00:00:00
abstract：:Hepatocyte cell death is a key process in the pathogenesis of nonalcoholic steatohepatitis (NASH). However, the factors responsible for and mechanisms underlying NASH-related cell death have not yet been elucidated in detail. We herein investigated the effects of intracellular glyceraldehyde (GA)-derived advanced glyc...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21144861
更新日期：2020-07-09 00:00:00
abstract：:Patients with brain metastasis from renal cell carcinoma (RCC) or melanoma have historically had very poor prognoses of less than one year. Stereotactic radiosurgery (SRS) can be an effective treatment for patients with these tumors. This study analyzes the effect of pretreatment prognostic factors on overall survival...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms17030400
更新日期：2016-03-18 00:00:00
abstract：:Consecutive treatment of adult rat heart with isoproterenol and adenosine (Iso/Aden), known to consecutively activate PKA/PKC signaling, is cardioprotective against ischemia and reperfusion (I/R). Whether this is cardioprotective in an immature heart is unknown. Langendorff-perfused hearts from adult and immature (60 ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms19020494
更新日期：2018-02-07 00:00:00
abstract：:C-repeat binding factor/dehydration-responsive element (CBF/DRE) transcription factors (TFs) participate in a variety of adaptive mechanisms, and are involved in molecular signaling and abiotic stress tolerance in plants. In pear (Pyrus pyrifolia) and other rosaceous crops, the independent evolution of CBF subfamily m...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20092074
更新日期：2019-04-26 00:00:00
abstract：:The yield losses in cereal crops because of abiotic stress and the expected huge losses from climate change indicate our urgent need for useful traits to achieve food security. The stay-green (SG) is a secondary trait that enables crop plants to maintain their green leaves and photosynthesis capacity for a longer time...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms20235837
更新日期：2019-11-20 00:00:00
abstract：:The aqueous solubility (logW) and n-octanol/water partition coefficient (logP(OW)) are important properties for pharmacology, toxicology and medicinal chemistry. Based on an understanding of the dissolution process, the frontier orbital interaction model was suggested in the present paper to describe the solvent-solut...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms9060962
更新日期：2008-06-01 00:00:00
abstract：:Twenty-one microsatellite markers from the genome of Cardiocrinum giganteum var. yunnanense, an important economic plant in China, were developed with a fast isolation protocol by amplified fragment length polymorphism of sequences containing repeats (FIASCO). Polymorphism within each locus was assessed in 24 wild ind...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms13021437
更新日期：2012-01-01 00:00:00
abstract：:There have been tens of thousands of RNAs deposited in different databases that contain sequences of two genes and are coined chimeric RNAs, or chimeras. However, "chimeric RNA" has never been lucidly defined, partly because "gene" itself is still ill-defined and because the means of production for many RNAs is unclea...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms18040714
更新日期：2017-03-28 00:00:00
abstract：:Diabetic nephropathy (DN) is the leading cause of end-stage renal disease in diabetes mellitus. Oxidative stress, insulin resistance and pro-inflammatory cytokines have been shown to play an important role in pathogeneses of renal damage on type 2 diabetes mellitus (DM). Inonotus obliquus (IO) is a white rot fungus th...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms17091535
更新日期：2016-09-13 00:00:00
abstract：:The recent thriving development of biobanks and associated high-throughput phenotyping studies requires the elaboration of large-scale approaches for monitoring biological sample quality and compliance with standard protocols. We present a metabolomic investigation of human blood samples that delineates pitfalls and g...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms17122035
更新日期：2016-12-05 00:00:00
abstract：:Tyrosinase (TYR) is a metalloenzyme classified as a type-3 copper protein, which is involved in the synthesis of melanin through a catalytic process beginning with the conversion of the amino acid l-Tyrosine (l-Tyr) to l-3,4-dihydroxyphenylalanine (l-DOPA). It plays an important role in the mechanism of melanogenesis ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21134783
更新日期：2020-07-06 00:00:00
