Abstract:
:Twenty-one microsatellite markers from the genome of Cardiocrinum giganteum var. yunnanense, an important economic plant in China, were developed with a fast isolation protocol by amplified fragment length polymorphism of sequences containing repeats (FIASCO). Polymorphism within each locus was assessed in 24 wild individuals from Gaoligong Mountains in western Yunnan Province, China. The number of alleles per locus ranged from 2 to 4 with a mean of 2.9. The expected and observed levels of heterozygosity ranged from 0.042 to 0.726 and from 0.000 to 1.000, with averages of 0.44 and 0.31, respectively. These polymorphic microsatellite markers should prove useful in population genetics studies and assessments of genetic variation to develop conservation and management strategies for this species.
journal_name
Int J Mol Scijournal_title
International journal of molecular sciencesauthors
Li R,Yang J,Yang J,Dao Zdoi
10.3390/ijms13021437subject
Has Abstractpub_date
2012-01-01 00:00:00pages
1437-43issue
2issn
1422-0067pii
ijms-13-01437journal_volume
13pub_type
杂志文章abstract::Chemoradiation-based bladder preservation therapy (BPT) is currently a curative option for non-metastatic muscle-invasive bladder cancer (MIBC) patients at favorable risk or an alternative to radical cystectomy (RC) for those who are unfit for RC. In BPT, only patients who achieve complete response (CR) after chemorad...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms19092777
更新日期:2018-09-15 00:00:00
abstract::Cholesteatoma is a destructive and expanding growth of keratinizing squamous epithelium in the middle ear or petrous apex. The molecular and cellular processes of the pathogenesis of acquired middle ear cholesteatoma have not been fully understood. In this study, comparative proteomic analysis was conducted to investi...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms140714439
更新日期:2013-07-11 00:00:00
abstract:BACKGROUND:Patients with breast cancer-the deadliest cancer among women-are at constant risk of developing metastasis. Oxidative stress and hypoxia are common feature of tumor cells that can proliferate even in a resultant metabolic acidosis. Despite the low extracellular pH, intracellular pH of tumor cells remains rel...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms18040170
更新日期:2017-03-23 00:00:00
abstract::In the past few years, thorough investigation of chemical modifications operated in the cells on ribonucleic acid (RNA) molecules is gaining momentum. This new field of research has been dubbed "epitranscriptomics", in analogy to best-known epigenomics, to stress the potential of ensembles of RNA modifications to cons...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms21134684
更新日期:2020-06-30 00:00:00
abstract::Aging is a major risk factor of osteoarthritis, which is characterized by the degeneration of articular cartilage. CCN3, a member of the CCN family, is expressed in cartilage and has various physiological functions during chondrocyte development, differentiation, and regeneration. Here, we examine the role of CCN3 in ...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21207556
更新日期:2020-10-13 00:00:00
abstract::A whole-bacterium-based SELEX (Systematic Evolution of Ligands by Exponential Enrichment) procedure was adopted in this study for the selection of an ssDNA aptamer that binds to Bifidobacterium bifidum. After 12 rounds of selection targeted against B. bifidum, 30 sequences were obtained and divided into seven families...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms18050883
更新日期:2017-04-25 00:00:00
abstract::Response surface methodology (RSM) was employed to optimize the extracellular lipase production by Burkholderia sp. HL-10. Preliminary tests showed that olive oil, tryptone and Tween-80 exhibited significant effects on the lipase production. The optimum concentrations of these three components were determined using a ...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms131114889
更新日期:2012-11-13 00:00:00
abstract::The cluster Re6Se8I63- has been shown to induce preferential cell death of a hepatic carcinoma cell line, thus becoming a promising anti-cancer drug. Whether this cluster induces acute hemolysis or if it interacts with albumin remains unclear. The effect of acute exposure of human red blood cells to different concentr...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms16011728
更新日期:2015-01-13 00:00:00
abstract::Ramie (Boehmeria nivea L. Gaud), commonly known as China grass, is a perennial bast fiber plant of the Urticaceae. In China, ramie farming, industry, and trade provide income for about five million people. Drought stress severely affects ramie stem growth and causes a dramatic decrease in ramie fiber production. There...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms16023493
更新日期:2015-02-04 00:00:00
abstract::The whitefly Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) causes serious crop losses worldwide by transmitting viruses. We have previously shown that salicylic acid (SA)-related plant defenses directly affect whiteflies. In this study, we applied exogenous SA to tomato plants in order to investigate the interac...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms17071048
更新日期:2016-06-30 00:00:00
abstract::Glutamate is not only a neurotransmitter but also an important neurotoxin in central nervous system (CNS). Chronic elevation of glutamate induces both neuronal and glial cell apoptosis. However, its effect on astrocytes is complex and still remains unclear. In this study, we investigated whether morphine, a common opi...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms17101523
更新日期:2016-10-24 00:00:00
abstract::Multiple evidence in animal models and in humans suggest a beneficial role of cold physical plasma in wound treatment. Yet, risk assessment studies are important to further foster therapeutic advancement and acceptance of cold plasma in clinics. Accordingly, we investigated the longterm side effects of repetitive plas...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms18040868
更新日期:2017-04-19 00:00:00
abstract::Whether or how neural stem cells (NSCs) respond to toll-like receptor 4 (TLR4) in an inflammatory environment caused by traumatic brain injury (TBI) has not been understood. In the present study, association between TLR4 expression and NSCs proliferation in the hippocampus was investigated in a mouse model of TBI usin...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms150712651
更新日期:2014-07-17 00:00:00
abstract::Because angiogenesis is a major contributor to cancer progression and metastasis, it is an attractive target for cancer therapy. Although a diverse number of small compounds for anti-angiogenic therapy have been developed, severe adverse effects commonly occur, since small compounds can affect not only tumor endotheli...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms20235819
更新日期:2019-11-20 00:00:00
abstract::It has been suggested that GAP-43 (growth-associated protein) binds to various proteins in growing neurons as part of its mechanism of action. To test this hypothesis in vivo, differentiated N1E-115 neuroblastoma cells were labeled with [(35)S]-amino acids and were treated with a cleavable crosslinking reagent. The ce...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms9091753
更新日期:2008-09-01 00:00:00
abstract::Soil is a complex matrix where biotic and abiotic components establish a still unclear network involving bacteria, fungi, archaea, protists, protozoa, and roots that are in constant communication with each other. Understanding these interactions has recently focused on metagenomics, metatranscriptomics and less on met...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms21228455
更新日期:2020-11-11 00:00:00
abstract::KRAS oncogenic mutations are widespread in lung cancer and, because direct targeting of KRAS has proven to be challenging, KRAS-driven cancers lack effective therapies. One alternative strategy for developing KRAS targeted therapies is to identify downstream targets involved in promoting important malignant features, ...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21165806
更新日期:2020-08-13 00:00:00
abstract::Carotid atherosclerotic plaque rupture can lead to cerebrovascular accident (CVA). By comparing RNA-Seq data from vascular smooth muscle cells (VSMC) extracted from carotid atheroma surgically excised from a group of asymptomatic and symptomatic subjects, we identified more than 700 genomic variants associated with sy...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21249387
更新日期:2020-12-09 00:00:00
abstract::Mutations on epidermal growth factor receptor (EGFR) of adenocarcinomas of lung have been found to be associated with increased sensitivity to EGFR tyrosine kinase inhibitors and K-ras mutations may correlate with primary resistance. We aimed to explore the discordant mutation statuses of EGFR and K-ras between primar...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms17040524
更新日期:2016-04-07 00:00:00
abstract::Neuroblastoma (NB) is an aggressive, relapse-prone infancy tumor of the sympathetic nervous system and is the leading cause of death among preschool age diseases, so the search for novel therapeutic targets is crucial. Golgi phosphoprotein 3 (GOLPH3) has been reported to be involved in the development, and in the DNA ...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms20194764
更新日期:2019-09-25 00:00:00
abstract::The survival kinase protein kinase B (Akt) participates in the regulation of essential subcellular processes, e.g., proliferation, growth, survival, and apoptosis, and has a documented role in promoting resistance against genotoxic stress including radiotherapy, presumably by influencing the DNA damage response and DN...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms19082233
更新日期:2018-07-31 00:00:00
abstract::The FLOWERING LOCUS T (FT) gene plays crucial roles in regulating the transition from the vegetative to reproductive phase. To understand the molecular mechanism of reproduction, three homologous FT genes were isolated and characterized from Cymbidium sinense "Qi Jian Bai Mo", Cymbidium goeringii and Cymbidium ensifol...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms130911385
更新日期:2012-01-01 00:00:00
abstract::Sema3C protein, a member of the class 3 family of secreted semaphorins, play an important role in tumor development by regulating cell proliferation, migration, invasion, and angiogenesis processes. Depending on the type and malignancy grade of the tumor, Sema3C function remains controversial. In this study, we constr...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms20225672
更新日期:2019-11-12 00:00:00
abstract::S-equol is a major bacterial metabolite of the soy isoflavone daidzein. It is known to be a phytoestrogen that acts by binding to the nuclear estrogen receptors (ERs) that are expressed in various brain regions, including the cerebellum. However, the effects of S-equol on cerebellar development and function have not y...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms20205178
更新日期:2019-10-18 00:00:00
abstract::Single nucleotide polymorphisms (SNPs) are currently the marker of choice in a variety of genetic studies. Using the high resolution melting (HRM) genotyping approach, 101 gene-based SNP markers were developed for Apostichopus japonicus, a sea cucumber species with economic significance for the aquaculture industry in...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms13067080
更新日期:2012-01-01 00:00:00
abstract::Argininosuccinate synthetase (ASS), a key enzyme to synthesize arginine is down regulated in many tumors including hepatocellular carcinoma (HCC). Similar to previous reports, we have found the decrease in ASS expression in poorly differentiated HCC. These ASS(-) tumors are auxotrophic for arginine. Pegylated arginine...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms18061175
更新日期:2017-06-01 00:00:00
abstract::A series of gold(I) complexes of the general composition [Au(naza)(PPh₃)] (1-8) was prepared and thoroughly characterized (e.g., electrospray ionization (ESI) mass spectrometry and multinuclear nuclear magnetic resonance (NMR) spectroscopy). The N1-deprotonated anions of 7-azaindole or its derivatives (naza) are coord...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms17122084
更新日期:2016-12-11 00:00:00
abstract::The aim of the study was to verify if quantification of placental specific C19MC microRNAs in plasma exosomes would be able to differentiate during the early stages of gestation between patients subsequently developing pregnancy-related complications and women with the normal course of gestation and if this differenti...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms20122972
更新日期:2019-06-18 00:00:00
abstract::The genetic basis of the wide variation for nutritional traits in Brassica rapa is largely unknown. A new Recombinant Inbred Line (RIL) population was profiled using High Performance Liquid Chromatography (HPLC) and Nuclear Magnetic Resonance (NMR) analysis to detect quantitative trait loci (QTLs) controlling seed toc...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms140815561
更新日期:2013-07-25 00:00:00
abstract::Histone acetylation plays an important role in plant growth and development. Here, we investigated the effect of sodium butyrate (NaB), a histone deacetylase inhibitor, on adventitious shoot formation from protoplast-derived calli and cotyledon explants of tobacco (Nicotiana benthamiana) and tomato (Solanum lycopersic...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21228451
更新日期:2020-11-10 00:00:00