Abstract:
:Soil is a complex matrix where biotic and abiotic components establish a still unclear network involving bacteria, fungi, archaea, protists, protozoa, and roots that are in constant communication with each other. Understanding these interactions has recently focused on metagenomics, metatranscriptomics and less on metaproteomics studies. Metaproteomic allows total extraction of intracellular and extracellular proteins from soil samples, providing a complete picture of the physiological and functional state of the "soil community". The advancement of high-performance mass spectrometry technologies was more rapid than the development of ad hoc extraction techniques for soil proteins. The protein extraction from environmental samples is biased due to interfering substances and the lower amount of proteins in comparison to cell cultures. Soil sample preparation and extraction methodology are crucial steps to obtain high-quality resolution and yields of proteins. This review focuses on the several soil protein extraction protocols to date to highlight the methodological challenges and critical issues for the application of proteomics to soil samples. This review concludes that improvements in soil protein extraction, together with the employment of ad hoc metagenome database, may enhance the identification of proteins with low abundance or from non-dominant populations and increase our capacity to predict functional changes in soil.
journal_name
Int J Mol Scijournal_title
International journal of molecular sciencesauthors
Tartaglia M,Bastida F,Sciarrillo R,Guarino Cdoi
10.3390/ijms21228455subject
Has Abstractpub_date
2020-11-11 00:00:00issue
22issn
1422-0067pii
ijms21228455journal_volume
21pub_type
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journal_title:International journal of molecular sciences
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journal_title:International journal of molecular sciences
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doi:10.3390/ijms131114073
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journal_title:International journal of molecular sciences
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doi:10.3390/ijms21072315
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doi:10.3390/ijms21103458
更新日期:2020-05-14 00:00:00
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journal_title:International journal of molecular sciences
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更新日期:2021-01-15 00:00:00
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journal_title:International journal of molecular sciences
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journal_title:International journal of molecular sciences
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