Abstract：
:Aging is a major risk factor of osteoarthritis, which is characterized by the degeneration of articular cartilage. CCN3, a member of the CCN family, is expressed in cartilage and has various physiological functions during chondrocyte development, differentiation, and regeneration. Here, we examine the role of CCN3 in cartilage maintenance. During aging, the expression of Ccn3 mRNA in mouse primary chondrocytes from knee cartilage increased and showed a positive correlation with p21 and p53 mRNA. Increased accumulation of CCN3 protein was confirmed. To analyze the effects of CCN3 in vitro, either primary cultured human articular chondrocytes or rat chondrosarcoma cell line (RCS) were used. Artificial senescence induced by H2O2 caused a dose-dependent increase in Ccn3 gene and CCN3 protein expression, along with enhanced expression of p21 and p53 mRNA and proteins, as well as SA-β gal activity. Overexpression of CCN3 also enhanced p21 promoter activity via p53. Accordingly, the addition of recombinant CCN3 protein to the culture increased the expression of p21 and p53 mRNAs. We have produced cartilage-specific CCN3-overexpressing transgenic mice, and found degradative changes in knee joints within two months. Inflammatory gene expression was found even in the rib chondrocytes of three-month-old transgenic mice. Similar results were observed in human knee articular chondrocytes from patients at both mRNA and protein levels. These results indicate that CCN3 is a new senescence marker of chondrocytes, and the overexpression of CCN3 in cartilage may in part promote chondrocyte senescence, leading to the degeneration of articular cartilage through the induction of p53 and p21.
journal_name
Int J Mol Scijournal_title
International journal of molecular sciencesauthors
Kuwahara M,Kadoya K,Kondo S,Fu S,Miyake Y,Ogo A,Ono M,Furumatsu T,Nakata E,Sasaki T,Minagi S,Takigawa M,Kubota S,Hattori Tdoi
10.3390/ijms21207556subject
Has Abstractpub_date
2020-10-13 00:00:00issue
20issn
1422-0067pii
ijms21207556journal_volume
21pub_type
杂志文章abstract：:Microglia are the resident macrophages of the central nervous system (CNS) and play an important role in neuronal recovery by scavenging damaged neurons. However, overactivation of microglia leads to neuronal death that is associated with CNS disorders. Therefore, regulation of microglial activation has been suggested...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms150915512
更新日期：2014-09-02 00:00:00
abstract：:Granulocytic myeloid-derived suppressor cells (Gr-MDSCs) promote immune evasion and resistance to immunotherapeutics in a variety of malignancies. Our previous study showed that dual PI3K/mTOR inhibitor Dactolisib impaired the viability and immunosuppressive function of Gr-MDSCs, and significantly synergized with immu...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21072337
更新日期：2020-03-27 00:00:00
abstract：:Protein-protein interactions are important for the molecular understanding of the biological processes of proteins. The dimerization of bZIPs (basic leucine zipper proteins) is involved in modifying binding site specificities, altering dimer stability, and permitting a new set of specific protein-to-protein interactio...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms150610005
更新日期：2014-06-04 00:00:00
abstract：:Proteins have been used as building blocks to provide various supramolecular structures in efforts to develop nano-biomaterials possessing broad biological functionalities. A series of unique structures have been obtained from the engineering of hemoproteins which contain the iron porphyrin known as heme, as a prosthe...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms22031012
更新日期：2021-01-20 00:00:00
abstract：:Biomarkers for the early detection of pancreatic cancer are urgently needed. The aim of this pilot study was to evaluate changes in serum N-glycoproteins and their glycosylation status prior to clinical presentation of pancreatic cancer that may be potential biomarkers. Prediagnosis serum samples pooled according to f...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms18122670
更新日期：2017-12-09 00:00:00
abstract：:Alzheimer's disease (AD) is the leading cause of dementia worldwide. It involves progressive impairment of cognitive function. A growing number of neuroprotective compounds have been identified with potential anti-AD properties through in vitro and in vivo models of AD. Quercetin, a natural flavonoid contained in a wi...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21020493
更新日期：2020-01-13 00:00:00
abstract：:Neurons are particularly susceptible to microtubule (MT) defects and deregulation of the MT cytoskeleton is considered to be a common insult during the pathogenesis of neurodegenerative disorders. Evidence that dysfunctions in the MT system have a direct role in neurodegeneration comes from findings that several forms...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms21197354
更新日期：2020-10-05 00:00:00
abstract：:Phostensin is encoded by KIAA1949. 5'-RACEanalysis has been used to identify the translation start site of phostensin mRNA, indicating that it encodes 165 amino acids with an apparent molecular weight of 26 kDa on SDS-PAGE. This low-molecular-weight phostensin is present in human peripheral blood mononuclear cells and...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms15011068
更新日期：2014-01-15 00:00:00
abstract：:Brain metastases are the most prevalent of intracranial malignancies. They are associated with a very poor prognosis and near 100% mortality. This has been the case for decades, largely because we lack effective therapeutics to augment surgery and radiotherapy. Notwithstanding improvements in the precision and efficac...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms20061280
更新日期：2019-03-14 00:00:00
abstract：:The molecular pathology of diseases seen from the mitochondrial axis has become more complex with the progression of research. A variety of factors, including the failure of mitochondrial dynamics and quality control, have made it extremely difficult to narrow down drug discovery targets. We have identified MITOL (mit...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms21113781
更新日期：2020-05-27 00:00:00
abstract：:Inflammation and transforming growth factor-β1 (TGF-β1) contribute to the development of peritoneal fibrosis (PF), which is associated with peritoneal dialysis (PD). Astragalus membranaceus (Astragalus) has anti-inflammatory and anti-fibrotic effects in many diseases. The goal of this study was to determine the anti-f...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms150712959
更新日期：2014-07-22 00:00:00
abstract：:Stem cell-based therapy is a promising approach to treat cartilage lesions and clinical benefits have been reported in a number of studies. However, the efficacy of cell injection procedures may be impaired by cell manipulation and damage as well as by cell dissemination to non-target tissues. To overcome such issues,...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms19113322
更新日期：2018-10-25 00:00:00
abstract：:That the nervous system is the main target of lead (Pb) has long been considered an established fact until recent evidence has linked the Pb effect on the immune system to the toxic effects of Pb on the nervous system. In this paper, we present recent literature reports on the effect of Pb on the inflammatory processe...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms17122140
更新日期：2016-12-19 00:00:00
abstract：:Glyphodes pyloalis Walker (Lepidoptera: Pyralididae) is a serious pest in the sericulture industry, which has caused damage and losses in recent years. With the widespread use of insecticides, the insecticide resistance of G. pyloalis has becomes increasingly apparent. In order to find other effective methods to contr...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21051904
更新日期：2020-03-10 00:00:00
abstract：:Chaetomium globosum is frequently isolated in water-damaged buildings and produces two mycotoxins called chaetoglobosins A and C when cultured on building material. In this study, the influence of ambient pH on the growth of C. globosum was examined on an artificial medium. This fungus was capable of growth on potato ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms9122357
更新日期：2008-12-01 00:00:00
abstract：:Recent advances in the field of RNA research have provided compelling evidence implicating microRNA (miRNA) and long non-coding RNA molecules in many diverse and substantial biological processes, including transcriptional and post-transcriptional regulation of gene expression, genomic imprinting, and modulation of pro...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms13010840
更新日期：2012-01-01 00:00:00
abstract：:Increasing scientific evidence demonstrated the deregulation of human endogenous retroviruses (HERVs) expression in complex diseases, such as cancer, autoimmune, psychiatric, and neurological disorders. The dynamic regulation of HERV activity and their responsiveness to a variety of environmental stimuli designate HER...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms19113286
更新日期：2018-10-23 00:00:00
abstract：:The maintenance of ordinal cell cycle phases is a critical biological process in cancer genesis, which is a crucial target for anti-cancer drugs. As an important natural isoquinoline alkaloid from Chinese herbal medicine, Berberine (BBR) has been reported to possess anti-cancer potentiality to induce cell cycle arrest...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms19020327
更新日期：2018-01-23 00:00:00
abstract：:Accurate prediction of the potential hepatotoxic nature of new pharmaceuticals remains highly challenging. Therefore, novel in vitro models with improved external validity are needed to investigate hepatic metabolism and timely identify any toxicity of drugs in humans. In this study, we examined the effects of diclofe...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms17040584
更新日期：2016-04-16 00:00:00
abstract：:Parkinson's disease (PD) is caused by the loss of dopaminergic (DA) neurons in the midbrain substantia nigra (SN). Neuroinflammation, which is marked by microglial activation, plays a very important role in the pathogenesis of PD. Pro-inflammatory mediators produced by activated microglia could damage DA neurons. Henc...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms19010012
更新日期：2017-12-21 00:00:00
abstract：:Aeromonas veronii is a pathogen capable of infecting humans, livestock and aquatic animals, resulting in serious economic losses. In this study, two recombinant Lactobacillus casei expressing flagellin A (FlaA) of A. veronii, Lc-pPG-1-FlaA (surface-displayed) and Lc-pPG-2-FlaA (secretory) were constructed. The immune ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21010033
更新日期：2019-12-19 00:00:00
abstract：:A new chemical pathway yielding A-type vitisins in red wines is proposed herein from the reaction between anthocyanins and oxaloacetic acid (OAA). This new chemical path is thought to occur in the first stages of the wine production even during the fermentation process. This is due to the revealed high reactivity of O...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms18040762
更新日期：2017-04-04 00:00:00
abstract：:Bacillus cereus can survive in the form of spores for prolonged periods posing a serious problem for the manufacture of safe shelf-stable foods of optimal quality. Our study aims at increasing knowledge of B. cereus spores focusing primarily on germination mechanisms to develop novel milder food preservation strategie...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21155198
更新日期：2020-07-22 00:00:00
abstract：:With the development of molecular cloning technology and the deep understanding of antibody engineering, there are diverse bispecific antibody formats from which to choose to pursue the optimal biological activity and clinical purpose. The single-chain-based bispecific antibodies usually bridge tumor cells with immune...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms18010048
更新日期：2016-12-28 00:00:00
abstract：:Crown rot (CR), caused by various Fusarium species, is a major disease in many cereal-growing regions worldwide. Fusarium culmorum is one of the most important species, which can cause significant yield losses in wheat. A set of 126 advanced International Maize and Wheat Improvement Center (CIMMYT) spring bread wheat ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms19092666
更新日期：2018-09-08 00:00:00
abstract：:Saturated aqueous solutions of various common salts were examined for their effect on aqueous aldol reactions catalysted by a highly active C(2)-symmetric diprolinamide organocatalyst developed in our laboratory. With respect to the aldol reaction between cyclohexanone and 4-nitrobenzaldehyde, deionised water was alwa...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms12129083
更新日期：2011-01-01 00:00:00
abstract：:The mechanical component in the pathophysiology of dry eye disease (DED) deserves attention as an important factor. The lubrication deficit induced impaired mechano-transduction of lid pressure to the ocular surfaces may lead to the dysregulation of homeostasis in the epithelium, with sensations of pain and secondary ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms21124333
更新日期：2020-06-18 00:00:00
abstract：:Telomere length (TL) influences the development of lifestyle-related diseases, and neonatal TL may influence their prevalence. Various factors have been reported to affect neonatal TL. Although the fetus is exposed to multiple conditions in utero, the main factors affecting the shortening of neonatal TL are still not ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21249688
更新日期：2020-12-18 00:00:00
abstract：:The objective of this study was to investigate the underlying mechanisms behind the radiation-sensitising effects of the antennapedia proteins (ANTP)-smacN7 fusion protein on tumour cells. ANTP-SmacN7 fusion proteins were synthesised, and the ability of this fusion protein to penetrate cells was observed. Effects of r...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms141224087
更新日期：2013-12-11 00:00:00
abstract：:A series of novel 1-(4-substitutedbenzoyl)-4-(4-chlorobenzhydryl)piperazine derivatives 5a-g was designed by a nucleophilic substitution reaction of 1-(4-chlorobenzhydryl)piperazine with various benzoyl chlorides and characterized by elemental analyses, IR and (1)H nuclear magnetic resonance spectra. Cytotoxicity of t...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms13078071
更新日期：2012-01-01 00:00:00
