Abstract:
AIM:To establish a multiple detection method based on comparative polymerase chain reaction (cPCR) and ligase detection reaction (LDR)/ligase chain reaction (LCR) to quantify the intestinal bacterial components. METHODS:Comparative quantification of 16S rDNAs from different intestinal bacterial components was used to quantify multiple intestinal bacteria. The 16S rDNAs of different bacteria were amplified simultaneously by cPCR. The LDR/LCR was examined to actualize the genotyping and quantification. Two beneficial (Bifidobacterium, Lactobacillus) and three conditionally pathogenic bacteria (Enterococcus, Enterobacterium and Eubacterium) were used in this detection. With cloned standard bacterial 16S rDNAs, standard curves were prepared to validate the quantitative relations between the ratio of original concentrations of two templates and the ratio of the fluorescence signals of their final ligation products. The internal controls were added to monitor the whole detection flow. The quantity ratio between two bacteria was tested. RESULTS:cPCR and LDR revealed obvious linear correlations with standard DNAs, but cPCR and LCR did not. In the sample test, the distributions of the quantity ratio between each two bacterial species were obtained. There were significant differences among these distributions in the total samples. But these distributions of quantity ratio of each two bacteria remained stable among groups divided by age or sex. CONCLUSION:The detection method in this study can be used to conduct multiple intestinal bacteria genotyping and quantification, and to monitor the human intestinal health status as well.
journal_name
World J Gastroenteroljournal_title
World journal of gastroenterologyauthors
Tang ZR,Li K,Zhou YX,Xiao ZX,Xiao JH,Huang R,Gu GHdoi
10.3748/wjg.v18.i3.268subject
Has Abstractpub_date
2012-01-21 00:00:00pages
268-74issue
3eissn
1007-9327issn
2219-2840journal_volume
18pub_type
杂志文章abstract:AIM:To investigate whether butyrate or glutamine enemas could diminish inflammation in experimental diversion colitis. METHODS:Wistar specific pathogen-free rats were submitted to a Hartmann's end colostomy and treated with enemas containing glutamine, butyrate, or saline. Enemas were administered twice a week in the ...
journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v18.i32.4278
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journal_title:World journal of gastroenterology
pub_type: 杂志文章,评审
doi:10.3748/wjg.v24.i14.1521
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journal_title:World journal of gastroenterology
pub_type: 杂志文章
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pub_type: 杂志文章,评审
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更新日期:2007-08-07 00:00:00
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journal_title:World journal of gastroenterology
pub_type: 杂志文章
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pub_type: 杂志文章
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journal_title:World journal of gastroenterology
pub_type: 杂志文章
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journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v19.i15.2362
更新日期:2013-04-21 00:00:00
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journal_title:World journal of gastroenterology
pub_type: 社论,评审
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journal_title:World journal of gastroenterology
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doi:10.3748/wjg.v23.i3.533
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journal_title:World journal of gastroenterology
pub_type: 杂志文章,评审
doi:10.3748/wjg.v20.i39.14105
更新日期:2014-10-21 00:00:00
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journal_title:World journal of gastroenterology
pub_type: 杂志文章,评审
doi:10.3748/wjg.v25.i30.4158
更新日期:2019-08-14 00:00:00
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journal_title:World journal of gastroenterology
pub_type: 杂志文章
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journal_title:World journal of gastroenterology
pub_type: 杂志文章
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journal_title:World journal of gastroenterology
pub_type: 社论,评审
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journal_title:World journal of gastroenterology
pub_type: 杂志文章,评审
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更新日期:2015-06-21 00:00:00
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journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v19.i3.375
更新日期:2013-01-21 00:00:00
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journal_title:World journal of gastroenterology
pub_type: 杂志文章,评审
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更新日期:2014-06-28 00:00:00
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journal_title:World journal of gastroenterology
pub_type: 杂志文章
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更新日期:2016-09-21 00:00:00
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journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v12.i21.3410
更新日期:2006-06-07 00:00:00
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journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.15.2708
更新日期:2009-06-14 00:00:00
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journal_title:World journal of gastroenterology
pub_type: 杂志文章,随机对照试验
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journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v11.i31.4865
更新日期:2005-08-21 00:00:00
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journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v12.i25.4098
更新日期:2006-07-07 00:00:00
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journal_title:World journal of gastroenterology
pub_type: 社论,评审
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更新日期:2013-06-28 00:00:00