Abstract：
:In diploid eukaryotes, repair of double-stranded DNA breaks by homologous recombination often leads to loss of heterozygosity (LOH). Most previous studies of mitotic recombination in Saccharomyces cerevisiae have focused on a single chromosome or a single region of one chromosome at which LOH events can be selected. In this study, we used two techniques (single-nucleotide polymorphism microarrays and high-throughput DNA sequencing) to examine genome-wide LOH in a diploid yeast strain at a resolution averaging 1 kb. We examined both selected LOH events on chromosome V and unselected events throughout the genome in untreated cells and in cells treated with either γ-radiation or ultraviolet (UV) radiation. Our analysis shows the following: (1) spontaneous and damage-induced mitotic gene conversion tracts are more than three times larger than meiotic conversion tracts, and conversion tracts associated with crossovers are usually longer and more complex than those unassociated with crossovers; (2) most of the crossovers and conversions reflect the repair of two sister chromatids broken at the same position; and (3) both UV and γ-radiation efficiently induce LOH at doses of radiation that cause no significant loss of viability. Using high-throughput DNA sequencing, we also detected new mutations induced by γ-rays and UV. To our knowledge, our study represents the first high-resolution genome-wide analysis of DNA damage-induced LOH events performed in any eukaryote.
journal_name
Geneticsjournal_title
Geneticsauthors
St Charles J,Hazkani-Covo E,Yin Y,Andersen SL,Dietrich FS,Greenwell PW,Malc E,Mieczkowski P,Petes TDdoi
10.1534/genetics.111.137927subject
Has Abstractpub_date
2012-04-01 00:00:00pages
1267-84issue
4eissn
0016-6731issn
1943-2631pii
genetics.111.137927journal_volume
190pub_type
杂志文章相关文献
GENETICS文献大全abstract：:From the analysis of restriction maps of the Amy region in eight sibling species belonging to the Drosophila melanogaster species subgroup, we herein show that the patterns of duplication of the Amy gene are almost the same in all species. This indicates that duplication occurred before speciation within this species ...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1995-09-01 00:00:00
abstract：:Dosage compensation refers to the equal expression of X-linked genes despite the difference in copy number between the two sexes. The male-specific lethal (MSL) complex is concentrated on the X chromosome in males. A gene expression assay for embryos was developed to examine the function of this complex. In mutant mal...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.104.036020
更新日期：2005-04-01 00:00:00
abstract：:The K1 killer plasmid, [KIL-k1], of Saccharomyces cerevisiae is a 1.25 x 10(6) dalton linear double-stranded RNA plasmid coding for a protein toxin and immunity to that toxin. The [KIL-sd1] plasmid is a replication-defective mutant of [KIL-k1] that depends on one of the recessive chromosomal superkiller (ski-) mutatio...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1982-02-01 00:00:00
abstract：:DnaB is the helicase associated with the DNA polymerase III replication fork in Escherichia coli. Previously we observed that the dnaB107(ts) mutation, at its permissive temperature, greatly stimulated deletion events at chromosomal tandem repeats. This stimulation required recA, which suggests a recombinational mecha...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1999-05-01 00:00:00
abstract：:Differential expression of mRNA among animal strains is one of the mechanisms for their diversity. cDNA microarray analysis of the prostates of BUF/Nac (BUF) and ACI/N (ACI) rats, which show different susceptibility to prostate cancers, found 195 differentially expressed genes. To identify loci that control differenti...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.104.038174
更新日期：2005-11-01 00:00:00
abstract：:Additional experiments with homologous as well as heterologous hybridization confirmed our previous finding in Sciara coprophila that XX females have nearly twice the number of ribosomal RNA cistrons as XO males. A comparison between two different X' chromosomes revealed that only the one carrying the irradiation-indu...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1976-05-01 00:00:00
abstract：:The F(1) hybrids of Nicotiana suaveolens (subgenus Petunioides, 2n = 32) and N. glutinosa (subgenus Tabacum, 2n = 24), were examined during their development, from seedlings to mature plants. It was observed that in the hybrids, there was a progressive change of dominant N. glutinosa morphological characteristics towa...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1973-04-01 00:00:00
abstract：:Boundaries (insulators) in the Drosophila bithorax complex (BX-C) delimit autonomous regulatory domains that orchestrate the parasegment (PS)-specific expression of the BX-C homeotic genes. The Fab-7 boundary separates the iab-6 and iab-7 regulatory domains, which control Abd-B expression in PS11 and PS12, respectivel...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.118.301259
更新日期：2018-10-01 00:00:00
abstract：:A supposed sporulation-deficient mutation of Saccharomyces cerevisiae is found to affect mating in haploids and in diploids, and to be inseparable from the mating-type locus by recombination. The mutation is regarded as a defective a allele and is designated a*. This is confirmed by its dominance relations in diploids...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1976-02-01 00:00:00
abstract：:Mutants at the snail locus are zygotically acting embryonic lethals that affect dorsoventral patterning. A comparison of seven mutant alleles shows considerable variation in expressivity and a graded effect along the dorsoventral axis: more extreme alleles result in the abnormal development of the dorsally derived ect...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1984-10-01 00:00:00
abstract：:Electrophoretic comparisons of 40S and 55S ribosomal subunit proteins from 18 strains of Drosophila melanogaster revealed the virtual absence of allelic variation. More detailed two-dimensional studies on the large subunit proteins in 6 of the strains demonstrated additional complexity but still no interstrain variati...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1974-12-01 00:00:00
abstract：:Previous studies on gene expression in aneuploids revealed numerous trans-acting dosage effects. Segmental aneuploidy of each varied chromosomal region exhibited predominantly inverse effects on several target genes. Here, dosage regulation was examined in a maize (Zea mays L.) ploidy series where the complete genomic...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1996-04-01 00:00:00
abstract：:A genetic map for the model legume Lotus japonicus has been developed. The F(2) mapping population was established from an interspecific cross between L. japonicus and L. filicaulis. A high level of DNA polymorphism between these parents was the source of markers for linkage analysis and the map is based on a framewor...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：2002-08-01 00:00:00
abstract：:Environmental toxicants are chemicals that negatively affect human health. Although there are numerous ways to limit exposure, the ubiquitous nature of certain environmental toxicants makes it impossible to avoid them entirely. Consequently, scientists are continuously working toward developing strategies for combatin...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.120.303577
更新日期：2020-12-01 00:00:00
abstract：:Gene mapping studies to determine the order of alpha- and beta-interferon (Ifa, Ifb), aminolevulinate dehydratase (Lv), and alpha-1 acid glycoprotein-1 and -2 (Orm-1, Orm-2) relative to each other and to the reference genes brown (b), B-cell maturation factor responsiveness (Bmfr-1), and major urinary protein-1 (Mup-1...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1986-12-01 00:00:00
abstract：:In Drosophila melanogaster, the female sexual development of the soma and the germline requires the activity of the gene Sxl. The somatic cells need the function of the gene fl(2)d to follow the female developmental pathway, due to its involvement in the female-specific splicing of Sxl RNA. Here we report the analysis...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1992-03-01 00:00:00
abstract：:The L1 family of single-pass transmembrane cell adhesion molecules (L1CAMs) is conserved from Caenorhabditis elegans and Drosophila to vertebrates and is required for axon guidance, neurite outgrowth, and maintenance of neuronal positions. The extracellular region of L1CAMs mediates cell adhesion via interactions with...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.108.091272
更新日期：2008-11-01 00:00:00
abstract：:Laboratory crosses between wild strains of the two European house mouse subspecies Mus musculus domesticus (2n = 34) and M. m. musculus (2n = 40) were performed to analyze the selective processes involved in the non-introgression of centromeric regions of Robertsonian (Rb) fusions in the Danish hybrid zone. The chromo...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1997-11-01 00:00:00
abstract：:We developed stochastic population genetic theory for mitochondrial and chloroplast genes, using an infinite alleles model appropriate for molecular genetic data. We considered the effects of mutation, random drift, and migration in a finite island model on selectively neutral alleles. Recurrence equations were obtain...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1989-03-01 00:00:00
abstract：:Within the context of a conservation program the management of subdivided populations implies a compromise between the control of the global genetic diversity, the avoidance of high inbreeding levels, and, sometimes, the maintenance of a certain degree of differentiation between subpopulations. We present a dynamic an...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.107.083816
更新日期：2008-05-01 00:00:00
abstract：:Protein aggregates are a common pathological feature of most neurodegenerative diseases (NDs). Understanding their formation and regulation will help clarify their controversial roles in disease pathogenesis. To date, there have been few systematic studies of aggregates formation in Drosophila, a model organism that h...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.109.112516
更新日期：2010-04-01 00:00:00
abstract：:The pattern of mutational covariance among traits plays a central, but largely untested, role in many theories in evolutionary genetics. Here we estimate the pattern of phenotypic, environmental, and mutational correlations for a set of life-history, behavioral, and morphological traits using 67 self-fertilizing lines...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.104.040022
更新日期：2005-06-01 00:00:00
abstract：:The decrease of meiotic exchanges (crossing over and conversion) in two mutants of Sordaria macrospora correlated strongly with a reduction of chiasmata and of both types of "recombination nodules." Serial section reconstruction electron microscopy was used to compare the synapsis pattern of meiotic prophase I in wild...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1992-09-01 00:00:00
abstract：:To develop a catalog of regulatory sites in two major model organisms, Drosophila melanogaster and Caenorhabditis elegans, the modERN (model organism Encyclopedia of Regulatory Networks) consortium has systematically assayed the binding sites of transcription factors (TFs). Combined with data produced by our predecess...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.117.300657
更新日期：2018-03-01 00:00:00
abstract：:Pathogen resistance is an ecologically important phenotype increasingly well understood at the molecular genetic level. In this article, we examine levels of avrRpt2-dependent resistance and Rps2 locus DNA sequence variability in a worldwide sample of 27 accessions of Arabidopsis thaliana. The rooted parsimony tree of...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：2003-02-01 00:00:00
abstract：:Fossil evidence links human ancestry with populations that evolved from modern gracile morphology in Africa 130,000-160,000 years ago. Yet fossils alone do not provide clear answers to the question of whether the ancestors of all modern Homo sapiens comprised a single African population or an amalgamation of distinct ...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.105.041095
更新日期：2005-08-01 00:00:00
abstract：:Crossovers (COs) between homologous chromosomes are critical for meiotic chromosome segregation and form in the context of the synaptonemal complex (SC), a meiosis-specific structure that assembles between aligned homologs. During Caenorhabditis elegans meiosis, central region components of the SC (SYP proteins) are e...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.119.302625
更新日期：2019-10-01 00:00:00
abstract：:Resolving the mechanistic and genetic bases of reproductive barriers between species is essential to understanding the evolutionary forces that shape speciation. Intrinsic hybrid incompatibilities are often treated as fixed between species, yet there can be considerable variation in the strength of reproductive isolat...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.118.300840
更新日期：2018-07-01 00:00:00
abstract：:Four species of the Hawaiian Drosophila planitibia subgroup which are homosequential in their polytene chromosomes are resident on the islands of Molokai, Maui and Hawaii. Comparisons of DNA sequence divergence in these four have been made by hybridization of total single-copy radiolabeled tracer DNA from each of the ...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1983-06-01 00:00:00
abstract：:A unique aspect of meiosis is the segregation of homologous chromosomes at the meiosis I division. The pairing of homologous chromosomes is a critical aspect of meiotic prophase I that aids proper disjunction at anaphase I. We have used a site-specific recombination assay in Saccharomyces cerevisiae to examine allelic...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.105.050658
更新日期：2006-07-01 00:00:00
