Abstract:
:The human heart cannot regenerate after an injury. Lost cardiomyocytes are replaced by scar tissue resulting in reduced cardiac function causing high morbidity and mortality. One possible solution to this problem is cardiac tissue engineering. Here, we have investigated the suitability of non-mulberry silk protein fibroin from Indian tropical tasar Antheraea mylitta as a scaffold for engineering a cardiac patch in vitro. We have tested cell adhesion, cellular metabolic activity, response to extracellular stimuli, cell-to-cell communication and contractility of 3-days postnatal rat cardiomyocytes on silk fibroin. Our data demonstrate that A. mylitta silk fibroin exhibits similar properties as fibronectin, a component of the natural matrix for cardiomyocytes. Comparison to mulberry Bombyx mori silk protein fibroin shows that A. mylitta silk fibroin is superior probably due to its RGD domains. 3D scaffolds can efficiently be loaded with cardiomyocytes resulting in contractile patches. In conclusion, our findings demonstrate that A. mylitta silk fibroin 3D scaffolds are suitable for the engineering of cardiac patches.
journal_name
Biomaterialsjournal_title
Biomaterialsauthors
Patra C,Talukdar S,Novoyatleva T,Velagala SR,Mühlfeld C,Kundu B,Kundu SC,Engel FBdoi
10.1016/j.biomaterials.2011.12.036subject
Has Abstractpub_date
2012-03-01 00:00:00pages
2673-80issue
9eissn
0142-9612issn
1878-5905pii
S0142-9612(11)01506-7journal_volume
33pub_type
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