Abstract:
:Methicillin-resistant Staphylococcus aureus (MRSA) with exogenous cassette DNA containing the methicillin-resistant gene mecA (SCCmec) poses a problem as a drug-resistant bacterium responsible for hospital- and community-acquired infections. The frequency of MRSA detection has recently been increasing rapidly in Japan, and SCCmec has also been classified more diversely into types I-V. A rapid test is essential for early diagnosis and treatment of MRSA infections, but detection by conventional methods requires at least two days. The newly developed multiplex PCR lateral flow method allows specific amplification of femA to detect S. aureus, mecA to detect SCCmec, and kdpC to detect SCCmec type II; moreover, PCR products can be evaluated visually in about 3 h. In the present study, we developed a PCR lateral flow method for MRSA using this method and investigated its clinical usefulness in the detection of MRSA. The results showed a diagnostic concordance rate of 91.7% for MRSA and methicillin-susceptible S. aureus between bacteriological examination and PCR lateral flow, and a high level of specificity in PCR lateral flow. In addition, a higher detection rate for S. aureus using the same sample was observed for PCR lateral flow (70.2%) than for bacteriological tests (48.6%). The above results show that PCR lateral flow for MRSA detection has high sensitivity, specificity, and speed, and its clinical application as a method for early diagnosis of MRSA infections appears to be feasible.
journal_name
Inflammationjournal_title
Inflammationauthors
Nihonyanagi S,Kanoh Y,Okada K,Uozumi T,Kazuyama Y,Yamaguchi T,Nakazaki N,Sakurai K,Hirata Y,Munekata S,Ohtani S,Takemoto T,Bandoh Y,Akahoshi Tdoi
10.1007/s10753-011-9395-4subject
Has Abstractpub_date
2012-06-01 00:00:00pages
927-34issue
3eissn
0360-3997issn
1573-2576journal_volume
35pub_type
杂志文章相关文献
INFLAMMATION文献大全abstract::We have characterized leukocyte migration to the pleural cavity in a methylated-BSA (mBSA)-induced model of murine delayed-type hypersensitivity and evaluated the ability of IL-4 and IL-10 to modulate this response. Neutrophils, macrophages, T cells, and dendritic cells migrated to the pleural cavity in a time-depende...
journal_title:Inflammation
pub_type: 杂志文章
doi:10.1023/a:1025076111950
更新日期:2003-08-01 00:00:00
abstract::Interleukin-10 (IL-10) is a pleiotropic cytokine and plays an important role in the pathogenesis of systemic lupus erythematosus (SLE). Unlike IL-10 protein, few studies have focused on the potential association between IL-10 receptor (IL-10R) and SLE. The purpose of this study was to examine the association of a sing...
journal_title:Inflammation
pub_type: 杂志文章
doi:10.1007/s10753-013-9658-3
更新日期:2013-12-01 00:00:00
abstract::Bee venom (BV) is used in folk medicine to treat arthritis. It has antiinflammatory effects in animal models of rheumatic disease. We have studied the effects of BV on human neutrophil production of superoxide (O2-) and hydrogen peroxide, finding potent, nontoxic, dose-dependent production inhibition. Melittin, the ma...
journal_title:Inflammation
pub_type: 杂志文章
doi:10.1007/BF00915999
更新日期:1986-06-01 00:00:00
abstract::Ischemic stroke is a major cause of death and motor disabilities all over the world. It is a muti-factorial disorder associated with inflammatory, apoptotic, and oxidative responses. Nateglinide (NAT), an insulinotropic agent used for the treatment of type 2 diabetes mellitus, recently showed potential anti-inflammato...
journal_title:Inflammation
pub_type: 杂志文章
doi:10.1007/s10753-019-01154-3
更新日期:2020-04-01 00:00:00
abstract::Baicalein shows anti-inflammatory effects in human rheumatoid arthritis fibroblast-like synoviocytes (RAFLS). Considering its anti-proliferatory effects on various cancer cells, we investigated the effects of baicalein on interleukin-1 beta (IL-1β)-induced proliferation of human RAFLS. Cell proliferation was examined ...
journal_title:Inflammation
pub_type: 杂志文章
doi:10.1007/s10753-013-9725-9
更新日期:2014-02-01 00:00:00
abstract::We analyzed the effect of the PAF receptor antagonist (+)-cis-3,5-dimethyl-2-(3-pyridyl)thiazolidin-4-one hydrochloride (SM-12502) on human platelet aggregation as well as mediator release. After incubation of human platelet with different concentrations of SM-12502 the cells were subsequently stimulated with either t...
journal_title:Inflammation
pub_type: 杂志文章
doi:10.1007/BF01487746
更新日期:1996-02-01 00:00:00
abstract::Platelet factor 4 (PF4) was the first discovered CXC chemokine and is found in platelet granules at very high concentration. Now, it is becoming increasingly evident that PF4 actively participates in inflammation and immune response. Recent paper demonstrated that PF4 limits the development and response of the Th17 ce...
journal_title:Inflammation
pub_type: 杂志文章
doi:10.1007/s10753-014-9903-4
更新日期:2014-10-01 00:00:00
abstract::Statins are widely used hypocholesterolemic drugs that block the mevalonate pathway. Some studies have shown that statins may have the potential to inhibit intervertebral disk (IVD) degeneration (IDD). Interleukin (IL)-1β, a catabolic cytokine, is a key regulator of IDD. This study aimed to investigate the mechanism u...
journal_title:Inflammation
pub_type: 杂志文章
doi:10.1007/s10753-017-0516-6
更新日期:2017-06-01 00:00:00
abstract::DBA/1LacJ mice were immunized with type II collagen and boosted with bacterial lipopolysaccharide (LPS) 17 days later to induce accelerated arthritis. Clinical signs of inflammation were observed as early as Day 20. Matrix metalloproteinases MMP-2, -3, -9, and -13, but not MMP-12, mRNA levels were increased on Day 24....
journal_title:Inflammation
pub_type: 杂志文章
doi:10.1023/a:1023282701505
更新日期:2003-04-01 00:00:00
abstract::Injury to podocytes leads to proteinuria, a hallmark of most glomerular diseases as well as being associated with the progression of kidney disease. Activation of the Wnt/β-catenin pathway is associated with the pathogenesis of podocyte dysfunction and can play a role in renal injury. Furthermore, the expression of fr...
journal_title:Inflammation
pub_type: 杂志文章
doi:10.1007/s10753-019-00988-1
更新日期:2019-08-01 00:00:00
abstract::The role of peptide leukotrienes (p-LTs), especially LTC4 and LTD4 in liver disease, was investigated in mice experimental liver injury models. The liver injury was induced by the injection of bacterial lipopolysaccharide (LPS) into Corynebacterium parvum pretreated mice. Carbon tetrachloride (CCl4)-induced liver inju...
journal_title:Inflammation
pub_type: 杂志文章
doi:10.1007/BF00914311
更新日期:1989-12-01 00:00:00
abstract::Type I allergy is characterized by the release of granule-associated mediators, lipid-derived substances, cytokines, and chemokines by activated mast cells. To evaluate the anti-allergic effects of macelignan isolated from Myristica fragrans Houtt., we determined its ability to inhibit calcium (Ca(2+)) influx, degranu...
journal_title:Inflammation
pub_type: 杂志文章
doi:10.1007/s10753-012-9490-1
更新日期:2012-10-01 00:00:00
abstract::Chronic obstructive pulmonary disease (COPD), characterized by progressive airway inflammation and irreversible airflow limitation, leads to serious decline in life quality. The acute exacerbation of COPD (AECOPD) results in high healthcare costs as well as a significant mortality rate. The most common cause of acute ...
journal_title:Inflammation
pub_type: 杂志文章,评审
doi:10.1007/s10753-018-0830-7
更新日期:2018-10-01 00:00:00
abstract::Several experiments have detected that melatonin exerts a marked influence on the inflammatory process. In the present study we evaluated the effect of exogenous melatonin on the experimental granulomatous tissues in rat. Our data show that subcutaneous administration of different doses of melatonin given daily during...
journal_title:Inflammation
pub_type: 杂志文章
doi:10.1023/b:ifla.0000049043.89586.89
更新日期:2004-08-01 00:00:00
abstract::Asthma is a complex disease linked to various pathophysiological events, including proteinase activity. In this study, we examined whether a Diospyros blancoi methanolic extract (DBE) exerts protective effects on allergic asthma in a murine asthma model. To investigate the specific role of DBE, we employed a murine mo...
journal_title:Inflammation
pub_type: 杂志文章
doi:10.1007/s10753-011-9354-0
更新日期:2012-04-01 00:00:00
abstract::Valnemulin reportedly regulates inflammatory responses in addition to its in vitro antibacterial activity. In this study, we established a mouse model of lipopolysaccharide (LPS)-induced inflammatory lung injury and investigated the effect of valnemulin (100 mg/kg) on acute lung injury (ALI) 8 h after LPS challenge. W...
journal_title:Inflammation
pub_type: 杂志文章
doi:10.1007/s10753-010-9186-3
更新日期:2010-10-01 00:00:00
abstract::NSAIDs are widely used to alleviate the symptoms of OA. It remains controversial as to what effects these agents have on the progression of OA. In vitro studies showed several types of NSAIDs (e.g., sodium salicylate, indomethacin) inhibited the synthesis of cartilage matrix component, but some types of NSAIDs (e.g., ...
journal_title:Inflammation
pub_type: 杂志文章,评审
doi:10.1023/a:1015504632021
更新日期:2002-06-01 00:00:00
abstract::The phenotypic alterations between blood monocytes from 11 patients with end-stage renal disease, who had been on peritoneal dialysis for less than one week, and blood monocytes from 10 healthy controls, were analyzed. In addition, peritoneal macrophages in the dialysate effluent were enclosed. Analysis of functional ...
journal_title:Inflammation
pub_type: 杂志文章
doi:10.1023/a:1022395723972
更新日期:1998-02-01 00:00:00
abstract::Sepsis, a clinical syndrome occurring in patients following infection or injury, is a leading cause of morbidity and mortality worldwide. CD86 (B7-2) is a costimulatory molecule on antigen-presenting cells and plays critical roles in immune responses. In the current study, we investigated the association of two CD86 p...
journal_title:Inflammation
pub_type: 杂志文章
doi:10.1007/s10753-014-9997-8
更新日期:2015-04-01 00:00:00
abstract::The impact of several environmental and genetic factors on diabetes and its complications is well documented. It has also been established that cytokines play a key role in the regulation of immune responses which have been shown to be important in the pathogenesis of diabetes. Studies showed that single-nucleotide po...
journal_title:Inflammation
pub_type: 杂志文章
doi:10.1007/s10753-011-9376-7
更新日期:2012-06-01 00:00:00
abstract::Previous studies have suggested that Interleukin-6 (IL-6) acts as a marker of vasculitis. To determine the role of IL-6 in vasculitis we utilized two models of immune complex induced vascular injury (dermal Arthus and acute pulmonary alveolitis) in IL-6 deficient (IL-6(-/-)) and IL-6 sufficient (IL-6(+/+)) mice. Plasm...
journal_title:Inflammation
pub_type: 杂志文章
doi:10.1007/s10753-006-9011-1
更新日期:2005-12-01 00:00:00
abstract::D-limonene epoxidation generates (+)-limonene epoxide, an understudied compound in the pharmacologically point of view. Herein, we investigated the anti-inflammatory and antinociceptive potentialities of (+)-limonene epoxide and suggested a mechanism of action. The anti-inflammatory potential was analyzed using agents...
journal_title:Inflammation
pub_type: 杂志文章
doi:10.1007/s10753-016-0496-y
更新日期:2017-04-01 00:00:00
abstract::Various disease states are associated with eosinophilia and the release of eosinophil peroxidase (EPO) into the microenvironment. The present study targets the effects of low levels of EPO on macrophage (M phi) phagocytosis and intracellular killing of Candida albicans as well as M phi oxidative activity measured as t...
journal_title:Inflammation
pub_type: 杂志文章
doi:10.1023/a:1027366119901
更新日期:1997-04-01 00:00:00
abstract::We aim to determine the extent of the CD40-CD40L pathway involvement in activated platelet-induced changes in human umbilical endothelial cells (HUVECs). Activated platelets were co-incubated with HUVECs in the presence or absence of CD40LmAb. HUVECs were also directly stimulated with rhCD40L. HUVEC endothelial cycloo...
journal_title:Inflammation
pub_type: 杂志文章
doi:10.1007/s10753-011-9427-0
更新日期:2012-06-01 00:00:00
abstract::Recent data have demonstrated the importance of IL-18 in the induction and perpetuation of chronic inflammation in experimental arthritis. The aim of the present study was to elucidate whether IL-18 has any indirect effects on osteoclastogenesis by regulating the production of molecules from fibroblast-like synoviocyt...
journal_title:Inflammation
pub_type: 杂志文章
doi:10.1007/s10753-012-9524-8
更新日期:2013-02-01 00:00:00
abstract::Our study showed that S-methylisothiourea (SMT) had anti-inflammatory effects in treating herpes simplex encephalitis in mice, and SMT also induced apoptosis of herpes simplex virus (HSV-1)-infected microglial cells. Both animal and cell models were employed in this study. Both models included the following five group...
journal_title:Inflammation
pub_type: 杂志文章
doi:10.1007/s10753-010-9246-8
更新日期:2011-10-01 00:00:00
abstract::Inflammatory cells, macrophages induced by lipopolysaccharide (LPS) stimulation, lead to the production of inflammatory cytokines, which are crucial to host defense. MicroRNAs are short noncoding RNAs that regulate key biological processes via suppression of gene expression at posttranscriptional levels. Recently, miR...
journal_title:Inflammation
pub_type: 杂志文章
doi:10.1007/s10753-013-9713-0
更新日期:2014-02-01 00:00:00
abstract::Diabetic retinopathy (DR) is a serious-threatening complication of diabetes and urgently needed to be treated. Evidence has accumulated indicating that microglia inflammation within the retina plays a critical role in DR. Microglial matrix metalloproteinase 9 (MMP-9) has an important role in the destruction of the int...
journal_title:Inflammation
pub_type: 杂志文章
doi:10.1007/s10753-017-0571-z
更新日期:2017-10-01 00:00:00
abstract::Protamine sulfate induces enzyme secretion from rabbit neutrophils. Enzyme secretion is mainly due to exocytosis but, depending on the experimental conditions, a small amount of cytolysis may occur. As compared with stimulation of neutrophil functions by other activators, protamine sulfate-induced enzyme release by ex...
journal_title:Inflammation
pub_type: 杂志文章
doi:10.1007/BF00915825
更新日期:1986-12-01 00:00:00
abstract::Different studies have shown the regulatory effects of vitamin D(3) on the immune system and bone metabolism. Regarding the effects of vitamin D on immune cells and the importance of cytokines on bone metabolism, we assessed the association between serum levels of interleukin (IL)-6, IL-10, IL-12, IL-17 and IFN-γ cyto...
journal_title:Inflammation
pub_type: 杂志文章
doi:10.1007/s10753-012-9531-9
更新日期:2013-02-01 00:00:00