Abstract:
:Pectins are complex polysaccharides that are essential components of the plant cell wall. In this study, a novel putative Arabidopsis S-adenosyl-L-methionine (SAM)-dependent methyltransferase, termed QUASIMODO 3 (QUA3, At4g00740), has been characterized and it was demonstrated that it is a Golgi-localized, type II integral membrane protein that functions in methylesterification of the pectin homogalacturonan (HG). Although transgenic Arabidopsis seedlings with overexpression, or knock-down, of QUA3 do not show altered phenotypes or changes in pectin methylation, this enzyme is highly expressed and abundant in Arabidopsis suspension-cultured cells. In contrast, in cells subjected to QUA3 RNA interference (RNAi) knock-down there is less pectin methylation as well as altered composition and assembly of cell wall polysaccharides. Taken together, these observations point to a Golgi-localized QUA3 playing an essential role in controlling pectin methylation and cell wall biosynthesis in Arabidopsis suspension cell cultures.
journal_name
J Exp Botjournal_title
Journal of experimental botanyauthors
Miao Y,Li HY,Shen J,Wang J,Jiang Ldoi
10.1093/jxb/err211subject
Has Abstractpub_date
2011-10-01 00:00:00pages
5063-78issue
14eissn
0022-0957issn
1460-2431pii
err211journal_volume
62pub_type
杂志文章abstract::Voltage dependence of ionic currents and ion fluxes in a walled, turgor-regulating cell were measured in Neurospora crassa. The hyphal morphology of the model organism Neurospora simplifies cable analysis of ionic currents to determine current density for quantitative comparisons with ion fluxes. The ion fluxes were m...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erm204
更新日期:2007-01-01 00:00:00
abstract::Many plant species and/or genotypes are highly recalcitrant to Agrobacterium-mediated genetic transformation, and yet little is known about this phenomenon. Using several Arabidopsis genotypes/ecotypes, the results of this study indicated that phytohormone pretreatment could overcome this recalcitrance by increasing t...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jexbot/51.353.1961
更新日期:2000-12-01 00:00:00
abstract::Research into the molecular basis of plant-insect interactions is hampered by the inability to alter the expression of individual genes in plants growing under natural conditions. The ability of virus-induced gene silencing (VIGS) to silence the expression of two jasmonate-induced genes known to mediate the expression...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erh004
更新日期:2004-01-01 00:00:00
abstract::The mechanism of the post-translational modulation of nitrate reductase activity (NR, EC 1.6.6.1) is briefly summarized, and it is shown that by this mechanism nitric oxide production through NR is also rapidly modulated. New and partly unexpected details on the modulation mechanism have been obtained by using immunol...
journal_title:Journal of experimental botany
pub_type: 杂志文章,评审
doi:10.1093/jexbot/53.370.875
更新日期:2002-04-01 00:00:00
abstract::Increasing drought stress poses a severe threat to agricultural productivity. Plants, however, evolved numerous mechanisms to cope with such environmental stress. Here we report that the stress-induced production of a peptide signal contributes to stress tolerance. The expression of phytosulfokine (PSK) peptide precur...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erab017
更新日期:2021-01-20 00:00:00
abstract::Although the mechanisms of nodule N(2) fixation in legumes are now well documented, some uncertainty remains on the metabolic consequences of water deficit. In most cases, little consideration is given to other organs and, therefore, the coordinated changes in metabolism in leaves, roots, and nodules are not well know...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/ers367
更新日期:2013-02-01 00:00:00
abstract::Mutations affecting the Arabidopsis SWC6 gene encoding a putative orthologue of a component of the SWR1 chromatin remodelling complex in plants have been characterized. swc6 mutations cause early flowering, shortened inflorescence internodes, and altered leaf and flower development. These phenotypic defects resemble t...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erm332
更新日期:2008-01-01 00:00:00
abstract::Dormancy of hazel (Corylus avellana L.) seeds is alleviated by a chilling treatment during which cytological, hormonal, and biochemical changes occur. Phytic acid and phosphate mobilization have been examined during this treatment. Phytic acid accounted for 0.7% and up to 3.2% of dry weight in axiferous and cotyledona...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/eri032
更新日期:2005-02-01 00:00:00
abstract::Ureides dramatically accumulate in shoots of N(2)-fixing soybean (Glycine max L. Merr.) under water deficit and this accumulation is higher in cultivars that have N(2) fixation that is sensitive to water deficit. One possible explanation is that ureide accumulation is associated with a feedback inhibition of nitrogena...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:
更新日期:2000-08-01 00:00:00
abstract::Somatic embryogenesis in Arabidopsis is achieved by culturing bending-cotyledon embryos on a 2,4-D-containing induction medium for 14 d followed by a transfer on to a hormone-free development medium. Several genes orthologous to Arabidopsis SHOOTMERISTEMLESS (STM), CLAVATA 1 (CLV1), and ZWILLE (ZLL) were isolated from...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erq222
更新日期:2010-09-01 00:00:00
abstract::Photosynthesis has become a major trait of interest for cereal yield improvement as breeders appear to have reached the theoretical genetic limit for harvest index, the mass of grain as a proportion of crop biomass. Yield improvements afforded by the adoption of green revolution dwarfing genes to wheat and rice are be...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/eraa077
更新日期:2020-04-06 00:00:00
abstract::The gene encoding stilbene synthase (STS) plays a central role in many biochemical and physiological actions, and its metabolite resveratrol possesses broad-spectrum resistance to pathogens, as well as diverse pharmacological properties, notably an anticancer effect. Here, we report the expression analysis of the gene...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erq447
更新日期:2011-05-01 00:00:00
abstract::The integration of signals received by a cell, and their transduction to targets, is essential for all cellular responses. The cytoskeleton has been identified as a major target of signalling cascades in both animal and plant cells. Self-incompatibility (SI) in Papaver rhoeas involves an allele-specific recognition be...
journal_title:Journal of experimental botany
pub_type: 杂志文章,评审
doi:10.1093/jxb/erg003
更新日期:2003-01-01 00:00:00
abstract::Photosystem (PS) II is the multisubunit complex which uses light energy to split water, providing the reducing equivalents needed for photosynthesis. The complex is susceptible to damage from environmental stresses such as excess excitation energy and high temperature. This research investigated the in vivo photosynth...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/ers156
更新日期:2012-08-01 00:00:00
abstract::Plants of Lolium perenne L. were grown in sterile solution culture supplied with 2 mol m(-3) nitrogen as either nitrate or ammonium. Glutamine at 5 mol m(-3) was added to the nutrient solution of half the plants for 24 h. Root nitrate influx (at external nitrate concentrations 0-2000 mmol m(-3)) and amino acid concent...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erh066
更新日期:2004-03-01 00:00:00
abstract::The response of stomata to many environmental factors is well documented. Multiple signalling pathways for abscisic acid (ABA)-induced stomatal closure have been proposed over the last decades. However, it seems that exposure of a leaf for a long time (several days) to some environmental conditions generates a sort of...
journal_title:Journal of experimental botany
pub_type: 杂志文章,评审
doi:10.1093/jxb/ert192
更新日期:2013-09-01 00:00:00
abstract::Intact coconuts were germinated in situ and compared with excised zygotic embryos germinated in vitro. The growth of the embryonic tissue and their fatty acid compositions were measured. Haustoria, plumules and radicles of coconuts germinated in situ grew continuously and proportionately throughout the 120 d experimen...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jexbot/52.358.933
更新日期:2001-05-01 00:00:00
abstract::Nitrogen (N) management is a promising agronomic strategy to minimize cadmium (Cd) contamination in crops. However, it is unclear how N affects Cd uptake by plants. Wild-type and iron uptake-inefficient tomato (Solanum lycopersicum) mutant (T3238fer) plants were grown in pH-buffered hydroponic culture to investigate t...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/ers036
更新日期:2012-05-01 00:00:00
abstract::γ-Aminobutyric acid (GABA) is implicated in pollen tube growth, but the molecular and cellular mechanisms that it mediates are largely unknown. Here, it is shown that exogenous GABA modulates putative Ca(2+)-permeable channels on the plasma membranes of tobacco pollen grains and pollen tubes. Whole-cell voltage-clamp ...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/eru171
更新日期:2014-07-01 00:00:00
abstract::Iron is an essential element for most organisms. As an indispensable co-factor of many enzymes, iron is involved in various crucial metabolic processes, which are required for the survival of plants and pathogens. Conversely, excessive iron produces highly active reactive oxygen species (ROS) that is toxic to the cell...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/eraa516
更新日期:2020-11-08 00:00:00
abstract::The timing of seed germination is crucial for seed plants and is coordinated by internal and external cues, reflecting adaptations to different habitats. Physiological and molecular studies with lettuce and Arabidopsis thaliana have documented a strict requirement for light to initiate germination and identified many ...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erz146
更新日期:2019-06-28 00:00:00
abstract::Proanthocyanidins (PAs) are agronomically important biopolymers in higher plants composed primarily of catechin and epicatechin units. The biosynthesis of these natural products is regulated by transcription factors including proteins of the R2R3MYB class. To gain insight into the genetic control of the catechin and e...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erq344
更新日期:2011-01-01 00:00:00
abstract::Axenically grown Arabidopsis thaliana plants were analysed for the occurrence of trehalose. Using gas chromatography-mass spectrometry (GC-MS) analysis, trehalose was unambiguously identified in extracts from Arabidopsis inflorescences. In a variety of organisms, the synthesis of trehalose is catalysed by trehalose-6-...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jexbot/52.362.1817
更新日期:2001-09-01 00:00:00
abstract::Leaf senescence is a very important trait that limits yield and biomass accumulation of agronomic crops and reduces post-harvest performance and the nutritional value of horticultural crops. Significant advance in physiological and molecular understanding of leaf senescence has made it possible to devise ways of manip...
journal_title:Journal of experimental botany
pub_type: 杂志文章,评审
doi:10.1093/jxb/eru248
更新日期:2014-07-01 00:00:00
abstract::Fructans are water-soluble fructose oligomers and polymers that are based on sucrose, and have been implicated in protecting plants against water stress. Rice (Oryza sativa L.) is highly sensitive to chilling temperatures, and is not able to synthesize fructans. Two wheat fructan-synthesizing enzymes, sucrose:sucrose ...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erm367
更新日期:2008-01-01 00:00:00
abstract::The target of rapamycin (TOR) pathway has emerged as a central hub synchronizing plant growth according to the nutrient/energy status and environmental inputs. Molecular mechanisms through which TOR promotes plant growth involve the positive regulation of transcription of cell proliferation-associated genes, mRNA tran...
journal_title:Journal of experimental botany
pub_type: 杂志文章,评审
doi:10.1093/jxb/ery459
更新日期:2019-04-15 00:00:00
abstract::Polyunsaturated fatty acids (PUFAs) are powerful modulators of several animal ion channels. It is shown here that PUFAs strongly affect the activity of the Slow Vacuolar (SV) channel encoded by the plant TPC1 gene. The patch-clamp technique was applied to isolated vacuoles from carrot taproots and Arabidopsis thaliana...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/ers272
更新日期:2012-10-01 00:00:00
abstract::In Arabidopsis, 20 genes encode putative glutamate receptor-like proteins (AtGLRs). However, the functions of most genes are unknown. In this study, our results revealed that loss of function of AtGLR3.6 (atglr3.6-1) leads to reduced primary root growth and fewer lateral roots, whereas AtGLR3.6 overexpression induced ...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erv576
更新日期:2016-03-01 00:00:00
abstract::The protein encoded by the Arabidopsis At3g55360 gene was selected as a candidate for the enoyl reductase of the microsomal elongase system based on its homology to the Tsc13p protein of S. cerevisiae. The studies presented here demonstrate that heterologous expression of At3g55360 functionally complements the tempera...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erh061
更新日期:2004-02-01 00:00:00
abstract::We previously identified the W-box-like-4 (Wbl-4) element (GTAGTGACTCAT), one of six Wbl elements in the BjC-P promoter of the unusual chitinase gene BjCHI1 from Brassica juncea, as the core element responsive to fungal infection. Here, we report the isolation and characterization of the cognate transcription factor i...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erw240
更新日期:2016-08-01 00:00:00