Abstract:
:The gene encoding stilbene synthase (STS) plays a central role in many biochemical and physiological actions, and its metabolite resveratrol possesses broad-spectrum resistance to pathogens, as well as diverse pharmacological properties, notably an anticancer effect. Here, we report the expression analysis of the gene encoding STS and its promoter function from a powdery mildew (PM)-resistant Chinese wild Vitis pseudoreticulata, and compare it with two PM-susceptible cultivated grapevines, Vitis vinifera cvs. Carignane and Thompson Seedless. We show an unusual expression pattern of STS in V. pseudoreticulata, which differs markedly from that of the cultivated species. Sequence comparisons reveal that the genomic DNA sequences encoding STS in the three grapevines are highly conserved, but a novel residue mutation within the key motif of STS is solely present in V. pseudoreticulata. Moreover, the STS promoter in V. pseudoreticulata displays a significantly different structure from that found in the two V. vinifera. The three promoter-driven GUS differential expression patterns in transformed tobacco plants induced with Alternaria alternata, methyl jasmonate, and wounding indicated that the structurally different STS promoter of V. pseudoreticulata is responsible for its specific regulatory function. We also demonstrate that the expression of STS genes from their native promoters are functional in transformed tobacco and retain pathogen inducibility. Importantly, the genomic DNA-2 of V. pseudoreticulata under its native promoter shows good induction and the maximum level of resveratrol content. These findings further our understanding of the regulation of STS expression in a resistant grapevine and provide a new pathogen-inducible promoter system for the genetic improvement of plant disease resistance.
journal_name
J Exp Botjournal_title
Journal of experimental botanyauthors
Xu W,Yu Y,Zhou Q,Ding J,Dai L,Xie X,Xu Y,Zhang C,Wang Ydoi
10.1093/jxb/erq447subject
Has Abstractpub_date
2011-05-01 00:00:00pages
2745-61issue
8eissn
0022-0957issn
1460-2431pii
erq447journal_volume
62pub_type
杂志文章abstract::Hydration at low temperatures, commonly referred to as cold stratification, is widely used for releasing dormancy and triggering germination in a wide range of species including wheat. However, the molecular mechanism that underlies its effect on germination has largely remained unknown. Our previous studies showed th...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erw172
更新日期:2016-05-01 00:00:00
abstract::Tomato, melon, grape, peach, and strawberry primarily accumulate soluble sugars during fruit development. In contrast, kiwifruit (Actinidia Lindl. spp.) and banana store a large amount of starch that is released as soluble sugars only after the fruit has reached maturity. By integrating metabolites measured by gas chr...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/ert293
更新日期:2013-11-01 00:00:00
abstract::Mitochondria from plants, yeast, and animals each contain at least one peroxiredoxin (Prx) that is involved in peroxide detoxification and redox signalling. The supramolecular dynamics of atypical type II Prx targeted to the mitochondrion was addressed in pea. Microcalorimetric (ITC) titrations identified an extremely...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/ern177
更新日期:2008-01-01 00:00:00
abstract::Plants have fundamental dependences on nitrogen and sulfur and frequently have to cope with chronic limitations when their supply is sub-optimal. This study aimed at characterizing the metabolomic, proteomic, and transcriptomic changes occurring in Arabidopsis leaves under chronic nitrate (Low-N) and chronic sulfate (...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/eraa337
更新日期:2020-10-22 00:00:00
abstract::Narrow-leafed lupin (Lupinus angustifolius L.) is the predominant grain legume crop in southern Australia, contributing half of the total grain legume production of Australia. Its yield in Australia is hampered by a range of subsoil constraints. The adaptation of lupin genotypes to subsoil constraints may be improved ...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erw127
更新日期:2016-06-01 00:00:00
abstract::This study presents the characterization of the plasma membrane (PM) H+-ATPases in potato, focusing on their role in stolon and tuber development. Seven PM H+-ATPase genes were identified in the Solanum tuberosum genome, designated PHA1-PHA7. PHA genes show distinct expression patterns in different plant tissues and u...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erx284
更新日期:2017-10-13 00:00:00
abstract::Dormancy of hazel (Corylus avellana L.) seeds is alleviated by a chilling treatment during which cytological, hormonal, and biochemical changes occur. Phytic acid and phosphate mobilization have been examined during this treatment. Phytic acid accounted for 0.7% and up to 3.2% of dry weight in axiferous and cotyledona...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/eri032
更新日期:2005-02-01 00:00:00
abstract::Hydroponically grown spinach plants were deprived of an external source of sulphate after an initial period when the S-supply was sufficient. The time-course of events following this treatment was monitored. The first responses were found in the uptake and translocation of NO(3)(-) and the uptake of SO(4)(2-). The for...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:
更新日期:2001-01-01 00:00:00
abstract::Transgenic tobacco (Nicotiana tabacum cv. LA Burley 21) lines expressing three genes encoding enzymes thought to be critical for the efficient production of phytochelatins, (i) serine acetyltransferase (EC 2.3.1.30) involved in the production of O-acetylserine, the cysteine precursor, (ii) gamma-glutamylcysteine synth...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erj176
更新日期:2006-01-01 00:00:00
abstract::FRIGIDA (FRI), as the major regulator of flowering time in Arabidopsis accessions, can activate its target FLOWERING LOCUS C (FLC) to delay flowering before vernalization. In addition to FLC, other FRI targets also exist in Arabidopsis. Although leaves sense environmental cues to modulate flowering time, it is not kno...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erz287
更新日期:2019-10-15 00:00:00
abstract::Understanding how green sink strength is regulated in planta poses a difficult problem because non-structural carbohydrate (NSC) levels can have integrated, simultaneous feedback effects on photosynthesis, sugar uptake, and respiration that depend on specific NSC moieties. Photosynthetic gametophytes of the fern Cerat...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erq407
更新日期:2011-03-01 00:00:00
abstract::The promoter of the Arabidopsis thaliana L. AtEm1 gene encoding a late embryogenesis abundant protein was fused to the beta-glucuronidase reporter gene and introduced into Brassica napus. The promoter is highly active in the vascular tissues of embryo and pollen grains and also active in petals, sepals, caulinar leave...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jexbot/52.360.1587
更新日期:2001-07-01 00:00:00
abstract::The process of selecting certain desirable traits for plant breeding may compromise other potentially important traits, such as defences against pests; however, specific phenotypic changes occurring over the course of domestication are unknown for most domesticated plants. Cranberry (Vaccinium macrocarpon) offers a un...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erq466
更新日期:2011-05-01 00:00:00
abstract::In photosynthetic tissues of the CAM plant pineapple (Ananas comosus), storage of soluble sugars in the central vacuole during the daytime and their remobilization at night is required to provide carbon skeletons for nocturnal CO(2) fixation. However, soluble sugars produced photosynthetically must also be exported to...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/ern077
更新日期:2008-01-01 00:00:00
abstract::Plant annexins are ubiquitous, soluble proteins capable of Ca(2+)-dependent and Ca(2+)-independent binding to endomembranes and the plasma membrane. Some members of this multigene family are capable of binding to F-actin, hydrolysing ATP and GTP, acting as peroxidases or cation channels. These multifunctional proteins...
journal_title:Journal of experimental botany
pub_type: 杂志文章,评审
doi:10.1093/jxb/erm344
更新日期:2008-01-01 00:00:00
abstract::Ethylene, or its precursor 1-aminocyclopropane-1-carboxylic acid (ACC), can stimulate hypocotyl elongation in the light. It is questioned whether gibberellins (GAs) play a role in this response. Tests with light of different wavelengths demonstrated that the ethylene response depends on blue light and functional crypt...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erm288
更新日期:2007-01-01 00:00:00
abstract::The mechanism of the post-translational modulation of nitrate reductase activity (NR, EC 1.6.6.1) is briefly summarized, and it is shown that by this mechanism nitric oxide production through NR is also rapidly modulated. New and partly unexpected details on the modulation mechanism have been obtained by using immunol...
journal_title:Journal of experimental botany
pub_type: 杂志文章,评审
doi:10.1093/jexbot/53.370.875
更新日期:2002-04-01 00:00:00
abstract::Plants generate reactive oxygen species (ROS) as part of their metabolism and in response to various external stress factors, potentially causing significant damage to biomolecules and cell structures. During the course of evolution, plants have adapted to ROS toxicity, and use ROS as signalling messengers that activa...
journal_title:Journal of experimental botany
pub_type: 杂志文章,评审
doi:10.1093/jxb/erv044
更新日期:2015-05-01 00:00:00
abstract::Vivipary, the germination of seeds before they are shed from the parent plant, is a rare event in angiosperms involving complex ecophysiological processes. Pseudovivipary and cryptovivipary occur in approximately 30 (2%) species of the cactus family. A remarkable case of vivipary in Epiphyllum phyllanthus is described...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erm232
更新日期:2007-01-01 00:00:00
abstract::The relationship between photosynthetic energy conservation and thermal dissipation of light energy is considered, with emphasis on organisms which tolerate full desiccation without suffering photo-oxidative damage in strong light. As soon as water becomes available to dry poikilohydric organisms, they resume photosyn...
journal_title:Journal of experimental botany
pub_type: 杂志文章,评审
doi:10.1093/jxb/erj104
更新日期:2006-01-01 00:00:00
abstract::To investigate the relationship between a carotenoid profile and gene expression for carotenoid cleavage dioxygenases, three citrus varieties that exhibit different 9-cis-violaxanthin levels in their juice sacs, Satsuma mandarin (Citrus unshiu Marc.; a variety accumulating a low level of 9-cis-violaxanthin), Valencia ...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erj172
更新日期:2006-01-01 00:00:00
abstract::Root proliferation as a response to exploit zones of nutrient enrichment in soil has been demonstrated for a wide range of plant species. However, the effectiveness of this as a strategy to acquire nutrients is also dependent on interactions with the soil microbial community. Specifically, C-flow from roots modifies m...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erj214
更新日期:2006-01-01 00:00:00
abstract::Heavy metal pollution often occurs together with organic contaminants. Brassinosteroids (BRs) induce plant tolerance to several abiotic stresses, including phenanthrene (PHE) and cadmium (Cd) stress. However, the role of BRs in PHE+Cd co-contamination-induced stress amelioration is unknown. Here, the interactive effec...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/ers323
更新日期:2013-01-01 00:00:00
abstract::The present work describes the intrachloroplast localization and the changes that took place in the thylakoid and stroma-located superoxide dismutases (SOD, EC 1.15.1.1) and ascorbate peroxidases (APX, EC 1.11.1.11), in response to long-term NaCl stress in Pisum sativum L. cv. Puget plants. Native PAGE using high chlo...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erh013
更新日期:2004-01-01 00:00:00
abstract::Endomembrane traffic in eukaryotic cells functions partially as a means of communication; delivery of membrane in one direction has to be balanced with a reduction at the other end. This effect is typically the case during the defence against pathogens. To combat pathogens, cellular growth and differentiation are supp...
journal_title:Journal of experimental botany
pub_type: 杂志文章,评审
doi:10.1093/jxb/erx363
更新日期:2017-12-18 00:00:00
abstract::The rise in atmospheric [CO(2)] is associated with increasing air temperature. However, studies on plant responses to interactive effects of [CO(2)] and temperature are limited, particularly for leaf structural attributes. In this study, Eucalyptus saligna plants were grown in sun-lit glasshouses differing in [CO(2)] ...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/ers231
更新日期:2012-10-01 00:00:00
abstract::The aleurone layer of cereal grains is important biologically as well as nutritionally and economically. Here, current knowledge on the regulation of aleurone development is reviewed. Recent reports suggest that the control of aleurone development is more complex than earlier models portrayed. Multiple levels of genet...
journal_title:Journal of experimental botany
pub_type: 杂志文章,评审
doi:10.1093/jxb/erq372
更新日期:2011-03-01 00:00:00
abstract::Wounding of plants leads to endogenous rise of jasmonic acid (JA) accompanied with the expression of a distinct set of genes. Among them are those coding for the allene oxide cyclase (AOC) that catalyses a regulatory step in JA biosynthesis, and for 1-deoxy-D-xylulose 5-phosphate synthase 2 (DXS2), an enzyme involved ...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/ern145
更新日期:2008-01-01 00:00:00
abstract::Small non-coding RNAs are key post-transcriptional and transcriptional regulators of plant gene expression in angiosperm sporophytes. In recent years, gametophytic small RNAs have also been investigated, predominantly in Arabidopsis male gametophytes, revealing features in common with the sporophyte as well as some su...
journal_title:Journal of experimental botany
pub_type: 杂志文章,评审
doi:10.1093/jxb/erq399
更新日期:2011-03-01 00:00:00
abstract::The development and maturation of tomato fruits has received considerable attention because of both the uniqueness of such processes to the biology of plants and the importance of these fruits as a component of the human diet. Molecular and genetic analysis of fruit development, and especially ripening of fleshy fruit...
journal_title:Journal of experimental botany
pub_type: 杂志文章,评审
doi:10.1093/jxb/erj020
更新日期:2006-01-01 00:00:00