Competence of Arabidopsis thaliana genotypes and mutants for Agrobacterium tumefaciens-mediated gene transfer: role of phytohormones.

Abstract:

:Many plant species and/or genotypes are highly recalcitrant to Agrobacterium-mediated genetic transformation, and yet little is known about this phenomenon. Using several Arabidopsis genotypes/ecotypes, the results of this study indicated that phytohormone pretreatment could overcome this recalcitrance by increasing the transformation rate in the known recalcitrant genotypes. Transient expression of a T-DNA encoded ss-glucuronidase (GUS) gene and stable kanamycin resistance were obtained for the ten Arabidopsis genotypes tested as well as for the mutant uvh1 (up to 69% of petioles with blue spots and up to 42% resistant calli). Cultivation of Arabidopsis tissues on phytohormones for 2-8 d before co-cultivation with Agrobacterium tumefaciens significantly increased transient GUS gene expression by 2-11-fold and stable T-DNA integration with petiole explants. Different Arabidopsis ecotypes revealed differences in their susceptibility to Agrobacterium-mediated transformation and in their type of reaction to pre-cultivation (three types of reactions were defined by gathering ecotypes into three groups). The Arabidopsis uvh1 mutant described as defective in a DNA repair system showed slightly lower competence to transformation than did its progenitor Colombia. This reduced transformation competence, however, could be overcome by 4-d pre-culture with phytohormones. The importance of pre-cultivation with phytohormones for genetic transformation is discussed.

journal_name

J Exp Bot

authors

Chateau S,Sangwan RS,Sangwan-Norreel BS

doi

10.1093/jexbot/51.353.1961

keywords:

subject

Has Abstract

pub_date

2000-12-01 00:00:00

pages

1961-8

issue

353

eissn

0022-0957

issn

1460-2431

journal_volume

51

pub_type

杂志文章
  • Tomato SlDREB gene restricts leaf expansion and internode elongation by downregulating key genes for gibberellin biosynthesis.

    abstract::Plants have evolved and adapted to different environments. Dwarfism is an adaptive trait of plants that helps them avoid high-energy costs under unfavourable conditions. The role of gibberellin (GA) in plant development has been well established. Several plant dehydration-responsive element-binding proteins (DREBs) ha...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/ers295

    authors: Li J,Sima W,Ouyang B,Wang T,Ziaf K,Luo Z,Liu L,Li H,Chen M,Huang Y,Feng Y,Hao Y,Ye Z

    更新日期:2012-11-01 00:00:00

  • Molecular characterization and expression analysis of the Rab GTPase family in Vitis vinifera reveal the specific expression of a VvRabA protein.

    abstract::As a first step to investigate whether Rab GTPases are involved in grape berry development, the Vitis vinifera EST and gene databases were searched for members of the VvRab family. The grapevine genome was found to contain 26 VvRabs that could be distributed into all of the eight groups described in the literature for...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/ern132

    authors: Abbal P,Pradal M,Muniz L,Sauvage FX,Chatelet P,Ueda T,Tesniere C

    更新日期:2008-01-01 00:00:00

  • An Arabidopsis mitochondria-localized RRL protein mediates abscisic acid signal transduction through mitochondrial retrograde regulation involving ABI4.

    abstract::The molecular mechanisms of abscisic acid (ABA) signalling have been studied for many years; however, how mitochondria-localized proteins play roles in ABA signalling remains unclear. Here an Arabidopsis mitochondria-localized protein RRL (RETARDED ROOT GROWTH-LIKE) was shown to function in ABA signalling. A previous ...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erv356

    authors: Yao X,Li J,Liu J,Liu K

    更新日期:2015-10-01 00:00:00

  • The role of reactive oxygen species and nitric oxide in programmed cell death associated with self-incompatibility.

    abstract::Successful sexual reproduction often relies on the ability of plants to recognize self- or genetically-related pollen and prevent pollen tube growth soon after germination in order to avoid self-fertilization. Angiosperms have developed different reproductive barriers, one of the most extended being self-incompatibili...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章,评审

    doi:10.1093/jxb/erv083

    authors: Serrano I,Romero-Puertas MC,Sandalio LM,Olmedilla A

    更新日期:2015-05-01 00:00:00

  • Formation and possible roles of nitric oxide in plant roots.

    abstract::Nitric oxide has been reported to act as a signalling molecule in different plant tissues and to participate in a variety of physiological processes. It is produced by different enzymes and sources. The root-specific plasma membrane-bound enzymes forming NO from the substrates nitrate and nitrite are of particular int...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erj058

    authors: Stöhr C,Stremlau S

    更新日期:2006-01-01 00:00:00

  • Function of pea amino acid permease AAP6 in nodule nitrogen metabolism and export, and plant nutrition.

    abstract::Legumes fix atmospheric nitrogen through a symbiotic relationship with bacteroids in root nodules. Following fixation in pea (Pisum sativum L.) nodules, nitrogen is reduced to amino acids that are exported via the nodule xylem to the shoot, and in the phloem to roots in support of growth. However, the mechanisms invol...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/ery289

    authors: Garneau MG,Tan Q,Tegeder M

    更新日期:2018-10-12 00:00:00

  • Post-translational modifications of FLOWERING LOCUS C modulate its activity.

    abstract::Flowering Locus C (FLC) is a key floral repressor that precisely controls flowering time. The role of FLC has been extensively studied at the transcriptional level using molecular biological and epigenetic approaches. However, how FLC functions and how its stability is controlled at the post-translational level are on...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章,评审

    doi:10.1093/jxb/erw431

    authors: Kwak JS,Son GH,Song JT,Seo HS

    更新日期:2017-01-01 00:00:00

  • Activity and protein level of AO isoforms in pea plants (Pisum sativum L.) during vegetative development and in response to stress conditions.

    abstract::Among three AO isoforms detected in pea plants, the activity of PAO-1 was dominant in leaves of seedlings and young leaves of mature plants, while PAO-3 revealed the highest band intensity in old leaves and roots. PAO-1 and PAO-3 are homodimers consisting of 145 kDa and 140 kDa subunits, respectively, while PAO-2 is a...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erh134

    authors: Zdunek-Zastocka E,Omarov RT,Koshiba T,Lips HS

    更新日期:2004-06-01 00:00:00

  • The NPR1-dependent salicylic acid signalling pathway is pivotal for enhanced salt and oxidative stress tolerance in Arabidopsis.

    abstract::The role of endogenous salicylic acid (SA) signalling cascades in plant responses to salt and oxidative stresses is unclear. Arabidopsis SA signalling mutants, namely npr1-5 (non-expresser of pathogenesis related gene1), which lacks NPR1-dependent SA signalling, and nudt7 (nudix hydrolase7), which has both constitutiv...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/eru528

    authors: Jayakannan M,Bose J,Babourina O,Shabala S,Massart A,Poschenrieder C,Rengel Z

    更新日期:2015-04-01 00:00:00

  • Isolation and characterization of a new, full-length cellulose synthase cDNA, PtrCesA5 from developing xylem of aspen trees.

    abstract::Cellulose synthases (CesA) represent a group of beta-1,4 glycosyltransferases involved in cellulose biosynthesis. Recent molecular studies confirmed that a large CesA gene family encoding various CesA isoforms exists in plants. The isolation and characterization of a new, full-length CesA cDNA, PtrCesA5 from an econom...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erg232

    authors: Kalluri UC,Joshi CP

    更新日期:2003-09-01 00:00:00

  • Intron-regulated expression of SUVH3, an Arabidopsis Su(var)3-9 homologue.

    abstract::SU(VAR)3-9 proteins are key regulators of heterochromatin structure and function in plants, mammals, Drosophila, and yeast. In contrast to animals and fungi, plants contain numerous Su(var)3-9 homologues (SUVH), the members of which form a discrete subfamily. The SU(VAR)3-9 and SUVH proteins associate with heterochrom...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erl093

    authors: Casas-Mollano JA,Lao NT,Kavanagh TA

    更新日期:2006-01-01 00:00:00

  • Expression of characteristics of ammonium nutrition as affected by pH of the root medium.

    abstract::To study the effect of root-zone pH on characteristic responses of NH4+ -fed plants, soybeans (Glycine max¿L.¿ Merr. cv. Ransom) were grown in flowing solution culture for 21 d on four sources of N (1.0 mol m-3 NO3-, 0.67 mol m-3 NO3- plus 0.33 mol m-3 NH4+, 0.33 mol m-3 NO3- plus 0.67 mol m-3 NH4+, and 1.0 mol m-3 NH...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/42.2.189

    authors: Chaillou S,Vessey JK,Morot-Gaudry JF,Raper CD Jr,Henry LT,Boutin JP

    更新日期:1991-02-01 00:00:00

  • NH4+-stimulated and -inhibited components of K+ transport in rice (Oryza sativa L.).

    abstract::The disruption of K(+) transport and accumulation is symptomatic of NH(4)(+) toxicity in plants. In this study, the influence of K(+) supply (0.02-40 mM) and nitrogen source (10 mM NH(4)(+) or NO(3)(-)) on root plasma membrane K(+) fluxes and cytosolic K(+) pools, plant growth, and whole-plant K(+) distribution in the...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/ern190

    authors: Szczerba MW,Britto DT,Ali SA,Balkos KD,Kronzucker HJ

    更新日期:2008-01-01 00:00:00

  • The influence of temperature on within-canopy acclimation and variation in leaf photosynthesis: spatial acclimation to microclimate gradients among climatically divergent Acer rubrum L. genotypes.

    abstract::Leaf gas exchange and temperature response were measured to assess temperature acclimation within a tree canopy in climatically contrasting genotypes of Acer rubrum L. Over the course of two 50 d continuous periods, growth temperature was controlled within tree crowns and the steady-state rate of leaf gas exchange was...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erm177

    authors: Bauerle WL,Bowden JD,Wang GG

    更新日期:2007-01-01 00:00:00

  • Silencing of the ACC synthase gene ACACS2 causes delayed flowering in pineapple [Ananas comosus (L.) Merr.].

    abstract::Flowering is a crucial developmental stage in the plant life cycle. A number of different factors, from environmental to chemical, can trigger flowering. In pineapple, and other bromeliads, it has been proposed that flowering is triggered by a small burst of ethylene production in the meristem in response to environme...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erl167

    authors: Trusov Y,Botella JR

    更新日期:2006-01-01 00:00:00

  • High-level overexpression of the Arabidopsis HsfA2 gene confers not only increased themotolerance but also salt/osmotic stress tolerance and enhanced callus growth.

    abstract::Heat shock transcription factors (Hsfs) are the central regulators of the heat shock (HS) stress response in all eukaryotic organisms. HsfA2 is one of the Arabidopsis class A Hsfs, and the induction of HsfA2 expression in response to HS stress is highest among all 21 Arabidopsis Hsfs. In this study, it is reported tha...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erm184

    authors: Ogawa D,Yamaguchi K,Nishiuchi T

    更新日期:2007-01-01 00:00:00

  • A defence-related Olea europaea β-glucosidase hydrolyses and activates oleuropein into a potent protein cross-linking agent.

    abstract::Oleuropein, the major secoiridoid compound in olive, is involved in a sophisticated two-component defence system comprising a β-glucosidase enzyme that activates oleuropein into a toxic glutaraldehyde-like structure. Although oleuropein deglycosylation studies have been monitored extensively, an oleuropein β-glucosida...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erv002

    authors: Koudounas K,Banilas G,Michaelidis C,Demoliou C,Rigas S,Hatzopoulos P

    更新日期:2015-04-01 00:00:00

  • Water transport in plant cuticles: an update.

    abstract::The scale, mechanism, and physiological importance of cuticular transpiration were last reviewed in this journal 5 and 10 years ago. Progress in our basic understanding of the underlying processes and their physiological and structural determinants has remained frustratingly slow ever since. There have been major adva...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章,评审

    doi:10.1093/jxb/erl017

    authors: Kerstiens G

    更新日期:2006-01-01 00:00:00

  • Sorghum stay-green QTL individually reduce post-flowering drought-induced leaf senescence.

    abstract::Sorghum is an important source of food, feed, and biofuel, especially in the semi-arid tropics because this cereal is well adapted to harsh, drought-prone environments. Post-flowering drought adaptation in sorghum is associated with the stay-green phenotype. Alleles that contribute to this complex trait have been mapp...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erl225

    authors: Harris K,Subudhi PK,Borrell A,Jordan D,Rosenow D,Nguyen H,Klein P,Klein R,Mullet J

    更新日期:2007-01-01 00:00:00

  • Fertility of CMS wheat is restored by two Rf loci located on a recombined acrocentric chromosome.

    abstract::Cytoplasmic male sterility (CMS) results from incompatibility between nuclear and cytoplasmic genomes, and is characterized by the inability to produce viable pollen. The restoration of male fertility generally involves the introgression of nuclear genes, termed restorers of fertility (Rf). CMS has been widely used fo...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/eru388

    authors: Castillo A,Atienza SG,Martín AC

    更新日期:2014-12-01 00:00:00

  • Actin fringes of polar cell growth.

    abstract::The eukaryotic actin cytoskeleton is a highly dynamic framework that is involved in many biological processes, such as cell growth, division, morphology, and motility. G-actin polymerizes into microfilaments that associate into bundles, patches, and networks, which, in turn, organize into higher order structures that ...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章,评审

    doi:10.1093/jxb/erx195

    authors: Stephan OOH

    更新日期:2017-06-15 00:00:00

  • The role of biochars in sustainable crop production and soil resiliency.

    abstract::Biochar is a promising soil additive for use in support of sustainable crop production. However, the high level of heterogeneity in biochar properties and the variations in soil composition present significant challenges to the successful uptake of biochar technologies in diverse agricultural soils. An improved unders...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章,评审

    doi:10.1093/jxb/erz301

    authors: Jiang Z,Lian F,Wang Z,Xing B

    更新日期:2020-01-07 00:00:00

  • Temperature during the day, but not during the night, controls flowering of Phalaenopsis orchids.

    abstract::Phalaenopsis orchids are among the most valuable potted flowering crops commercially produced throughout the world because of their long flower life and ease of crop scheduling to meet specific market dates. During commercial production, Phalaenopsis are usually grown at an air temperature > or =28 degrees C to inhibi...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erl176

    authors: Blanchard MG,Runkle ES

    更新日期:2006-01-01 00:00:00

  • Overproduction of SAT and/or OASTL in transgenic plants: a survey of effects.

    abstract::The last steps of cysteine biosynthesis are catalysed by a bi-enzyme complex composed of serine acetyltransferase (SAT) and cysteine synthase, also called O-acetyl-serine (thiol) lyase (OASTL). SAT is responsible for the production of O-acetyl-serine (OAS) from serine and acetyl-coenzyme A, while OASTL catalyses the f...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章,评审

    doi:10.1093/jxb/erh151

    authors: Sirko A,Błaszczyk A,Liszewska F

    更新日期:2004-08-01 00:00:00

  • Long-distance signalling from roots to shoots assessed: the flooding story.

    abstract::Several kinds of signal may be generated when roots are exposed to an environmental stress. Some, but not all, are conveyed to shoots in the transpiration stream. Principles are summarized that may help establish experimentally the presence or intensity of root signals transported by transpiration. In many dryland spe...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章,评审

    doi:10.1093/jexbot/53.367.175

    authors: Jackson MB

    更新日期:2002-02-01 00:00:00

  • Overexpression of a putative Arabidopsis BAHD acyltransferase causes dwarfism that can be rescued by brassinosteroid.

    abstract::Plant growth and development are ensured through networks of complex regulatory schemes. Genetic approaches have been invaluable in dissecting these regulatory pathways. This study reports the isolation of a semi-dominant dwarf mutant designated abnormal shoot1-1 dominant (abs1-1D) through an Arabidopsis T-DNA activat...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/ers227

    authors: Wang M,Liu X,Wang R,Li W,Rodermel S,Yu F

    更新日期:2012-10-01 00:00:00

  • Photosynthetic flexibility in maize exposed to salinity and shade.

    abstract::C4 photosynthesis involves a close collaboration of the C3 and C4 metabolic cycles across the mesophyll and bundle-sheath cells. This study investigated the coordination of C4 photosynthesis in maize plants subjected to two salinity (50 and 100mM NaCl) treatments and one shade (20% of full sunlight) treatment. Photosy...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/eru130

    authors: Sharwood RE,Sonawane BV,Ghannoum O

    更新日期:2014-07-01 00:00:00

  • Inhibition of nitrate influx by glutamine in Lolium perenne depends upon the contribution of the HATS to the total influx.

    abstract::Plants of Lolium perenne L. were grown in sterile solution culture supplied with 2 mol m(-3) nitrogen as either nitrate or ammonium. Glutamine at 5 mol m(-3) was added to the nutrient solution of half the plants for 24 h. Root nitrate influx (at external nitrate concentrations 0-2000 mmol m(-3)) and amino acid concent...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erh066

    authors: Thornton B

    更新日期:2004-03-01 00:00:00

  • 3D lidar imaging for detecting and understanding plant responses and canopy structure.

    abstract::Understanding and diagnosing plant responses to stress will benefit greatly from three-dimensional (3D) measurement and analysis of plant properties because plant responses are strongly related to their 3D structures. Light detection and ranging (lidar) has recently emerged as a powerful tool for direct 3D measurement...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章,评审

    doi:10.1093/jxb/erl142

    authors: Omasa K,Hosoi F,Konishi A

    更新日期:2007-01-01 00:00:00

  • Two key polymorphisms in a newly discovered allele of the Vitis vinifera TPS24 gene are responsible for the production of the rotundone precursor α-guaiene.

    abstract::Rotundone was initially identified as a grape-derived compound responsible for the peppery aroma of Shiraz wine varieties. It has subsequently been found in black and white pepper and several other spices. Because of its potent aroma, the molecular basis for rotundone formation is of particular relevance to grape and ...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erv491

    authors: Drew DP,Andersen TB,Sweetman C,Møller BL,Ford C,Simonsen HT

    更新日期:2016-02-01 00:00:00