Virus-induced gene silencing of jasmonate-induced direct defences, nicotine and trypsin proteinase-inhibitors in Nicotiana attenuata.

Abstract:

:Research into the molecular basis of plant-insect interactions is hampered by the inability to alter the expression of individual genes in plants growing under natural conditions. The ability of virus-induced gene silencing (VIGS) to silence the expression of two jasmonate-induced genes known to mediate the expression of two potent direct defences (nicotine and proteinase inhibitors) that are produced in different tissues (roots and shoots, respectively) in Nicotiana attenuata is documented here. Fragments of consensus sequences of N. attenuata's putrescine N-methyltransferase (PMT) and trypsin inhibitor (TI) genes were cloned in sense, anti-sense and inverted repeat orientations into the Tobacco Rattle Virus (TRV) to trigger post-transcriptional gene silencing by Agrobacterium-mediated inoculation in plants previously elicited with methyl jasmonate (MeJA) or left as controls. MeJA treatment elicited 2.4- and 9.8-fold increases in the concentrations of nicotine and proteinase inhibitors, respectively, and inoculation with constructs containing appropriate genes inhibited these MeJA-induced increases and halved constitutive accumulations, regardless of the orientation of the gene fragment. Root PMT transcript levels were significantly elevated in MeJA-treated plants 10 h after elicitation, but not in plants inoculated with the appropriate TRV constructs 9 d prior to MeJA treatment, demonstrating that VIGS was responsible for the inhibition of these potent direct defences. While additional research is required to minimize the effects on plant growth and the risks of using such constructs in natural settings, it is concluded that VIGS has a potential to manipulate the expression of genes important for ecological interactions.

journal_name

J Exp Bot

authors

Saedler R,Baldwin IT

doi

10.1093/jxb/erh004

keywords:

subject

Has Abstract

pub_date

2004-01-01 00:00:00

pages

151-7

issue

395

eissn

0022-0957

issn

1460-2431

pii

erh004

journal_volume

55

pub_type

杂志文章
  • The rate of CO(2) assimilation controls the expression and activity of glutamine synthetase through sugar formation in sunflower (Helianthus annuus L) leaves.

    abstract::The expression and activity of glutamine synthetase (GS, EC 6.3.1.2) were examined in relation to the rate of CO2 assimilation in sunflower (Helianthus annuus L.) leaves. Intact plants were kept in the dark for 72 h and subsequently exposed to light under different atmospheric CO2 concentrations (100, 400 and 1200 mic...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erh017

    authors: Larios B,Aguera E,Cabello P,Maldonado JM,de la Haba P

    更新日期:2004-01-01 00:00:00

  • Mutations in the Arabidopsis SWC6 gene, encoding a component of the SWR1 chromatin remodelling complex, accelerate flowering time and alter leaf and flower development.

    abstract::Mutations affecting the Arabidopsis SWC6 gene encoding a putative orthologue of a component of the SWR1 chromatin remodelling complex in plants have been characterized. swc6 mutations cause early flowering, shortened inflorescence internodes, and altered leaf and flower development. These phenotypic defects resemble t...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erm332

    authors: Lázaro A,Gómez-Zambrano A,López-González L,Piñeiro M,Jarillo JA

    更新日期:2008-01-01 00:00:00

  • A comparison of the effects of IAA and 4-Cl-IAA on growth, proton secretion and membrane potential in maize coleoptile segments.

    abstract::The physiological activity of exogenous 4-Cl-IAA, as compared to IAA, was examined in maize coleoptile segments. It was found that in this model system 4-Cl-IAA is much more active in the stimulation of elongation than IAA. Simultaneous measurements of growth and external pH indicated that administration of either IAA...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jexbot/53.371.1089

    authors: Karcz W,Burdach Z

    更新日期:2002-05-01 00:00:00

  • The fruit ripening-related gene FaAAT2 encodes an acyl transferase involved in strawberry aroma biogenesis.

    abstract::Short-chain esters contribute to the blend of volatiles that define the strawberry aroma. The last step in their biosynthesis involves an alcohol acyltransferase that catalyses the esterification of an acyl moiety of acyl-CoA with an alcohol. This study identified a novel strawberry alcohol acyltransferase gene (FaAAT...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/ers120

    authors: Cumplido-Laso G,Medina-Puche L,Moyano E,Hoffmann T,Sinz Q,Ring L,Studart-Wittkowski C,Caballero JL,Schwab W,Muñoz-Blanco J,Blanco-Portales R

    更新日期:2012-06-01 00:00:00

  • Expression of FRIGIDA in root inhibits flowering in Arabidopsis thaliana.

    abstract::FRIGIDA (FRI), as the major regulator of flowering time in Arabidopsis accessions, can activate its target FLOWERING LOCUS C (FLC) to delay flowering before vernalization. In addition to FLC, other FRI targets also exist in Arabidopsis. Although leaves sense environmental cues to modulate flowering time, it is not kno...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erz287

    authors: Kong X,Luo L,Zhao J,Chen Q,Chang G,Huang J,Yang Y,Hu X

    更新日期:2019-10-15 00:00:00

  • Capture and use of solar radiation, water, and nitrogen by sugar beet (Beta vulgaris L.).

    abstract::Sugar beet is spring-sown for sugar production in most sugar beet-growing countries. It is grown as a vegetative crop and it accumulates yield (sugar) from very early in its growth cycle. As long as the sugar beet plants do not flower, the sugar accumulation period is indefinite and yield continues to increase. This p...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章,评审

    doi:10.1093/jxb/erp110

    authors: Jaggard KW,Qi A,Ober ES

    更新日期:2009-01-01 00:00:00

  • Extracellular beta-glucosidase activity in barley involved in the hydrolysis of ABA glucose conjugate in leaves.

    abstract::Abscisic acid conjugate concentrations increased in barley xylem sap under salinity, whereas it remained at a low level in the intercellular washing fluid (IWF) of barley primary leaves (Hordeum vulgare cv. Gerbel). Here it is shown that IWF contains beta-glucosidase activity which releases abscisic acid (ABA) from th...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:

    authors: Dietz KJ,Sauter A,Wichert K,Messdaghi D,Hartung W

    更新日期:2000-05-01 00:00:00

  • Silencing Sl-EBF1 and Sl-EBF2 expression causes constitutive ethylene response phenotype, accelerated plant senescence, and fruit ripening in tomato.

    abstract::The hormone ethylene regulates a wide range of plant developmental processes and EBF (EIN3-binding F-box) proteins were shown to negatively regulate the ethylene signalling pathway via mediating the degradation of EIN3/EIL proteins. The present study reports on the identification of two tomato F-box genes, Sl-EBF1 and...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erp332

    authors: Yang Y,Wu Y,Pirrello J,Regad F,Bouzayen M,Deng W,Li Z

    更新日期:2010-03-01 00:00:00

  • Functional relationship between mTERF4 and GUN1 in retrograde signaling.

    abstract::Plastid-to-nucleus retrograde signaling plays an important role in regulating the expression of photosynthesis-associated nuclear genes (PhANGs) in accordance with physiological demands on chloroplast biogenesis and function. Despite its fundamental importance, little is known about the molecular nature of the plastid...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erv525

    authors: Sun X,Xu D,Liu Z,Kleine T,Leister D

    更新日期:2016-06-01 00:00:00

  • Rapid disruption of nitrogen metabolism and nitrate transport in spinach plants deprived of sulphate.

    abstract::Hydroponically grown spinach plants were deprived of an external source of sulphate after an initial period when the S-supply was sufficient. The time-course of events following this treatment was monitored. The first responses were found in the uptake and translocation of NO(3)(-) and the uptake of SO(4)(2-). The for...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:

    authors: Prosser IM,Purves JV,Saker LR,Clarkson DT

    更新日期:2001-01-01 00:00:00

  • The plastid outer envelope protein OEP16 affects metabolic fluxes during ABA-controlled seed development and germination.

    abstract::Previously, the OEP16.1 channel pore in the outer envelope membrane of mature pea (Pisum sativum) chloroplasts in vitro has been characterized to be selective for amino acids. Isolation of OEP16.2, a second OEP16 isoform from pea, in the current study allowed membrane localization and gene expression of OEP16 to be fo...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/err375

    authors: Pudelski B,Schock A,Hoth S,Radchuk R,Weber H,Hofmann J,Sonnewald U,Soll J,Philippar K

    更新日期:2012-03-01 00:00:00

  • The kiwifruit lycopene beta-cyclase plays a significant role in carotenoid accumulation in fruit.

    abstract::The composition of carotenoids, along with anthocyanins and chlorophyll, accounts for the distinctive range of colour found in the Actinidia (kiwifruit) species. Lutein and beta-carotene are the most abundant carotenoids found during fruit development, with beta-carotene concentration increasing rapidly during fruit m...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erp218

    authors: Ampomah-Dwamena C,McGhie T,Wibisono R,Montefiori M,Hellens RP,Allan AC

    更新日期:2009-01-01 00:00:00

  • Transpiration efficiency: new insights into an old story.

    abstract::Producing more food per unit of water has never been as important as it is at present, and the demand for water by economic sectors other than agriculture will necessarily put a great deal of pressure on a dwindling resource, leading to a call for increases in the productivity of water in agriculture. This topic has b...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章,评审

    doi:10.1093/jxb/eru040

    authors: Vadez V,Kholova J,Medina S,Kakkera A,Anderberg H

    更新日期:2014-11-01 00:00:00

  • Housekeeping gene selection for real-time RT-PCR normalization in potato during biotic and abiotic stress.

    abstract::Plant stress studies are more and more based on gene expression. The analysis of gene expression requires sensitive, precise, and reproducible measurements for specific mRNA sequences. Real-time RT-PCR is at present the most sensitive method for the detection of low abundance mRNA. To avoid bias, real-time RT-PCR is r...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/eri285

    authors: Nicot N,Hausman JF,Hoffmann L,Evers D

    更新日期:2005-11-01 00:00:00

  • A multi-model framework for the Arabidopsis life cycle.

    abstract::Linking our understanding of biological processes at different scales is a major conceptual challenge in biology and aggravated by differences in research methods. Modelling can be a useful approach to consolidating our understanding across traditional research domains. The laboratory model species Arabidopsis is very...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/ery394

    authors: Zardilis A,Hume A,Millar AJ

    更新日期:2019-04-29 00:00:00

  • Expression of characteristics of ammonium nutrition as affected by pH of the root medium.

    abstract::To study the effect of root-zone pH on characteristic responses of NH4+ -fed plants, soybeans (Glycine max¿L.¿ Merr. cv. Ransom) were grown in flowing solution culture for 21 d on four sources of N (1.0 mol m-3 NO3-, 0.67 mol m-3 NO3- plus 0.33 mol m-3 NH4+, 0.33 mol m-3 NO3- plus 0.67 mol m-3 NH4+, and 1.0 mol m-3 NH...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/42.2.189

    authors: Chaillou S,Vessey JK,Morot-Gaudry JF,Raper CD Jr,Henry LT,Boutin JP

    更新日期:1991-02-01 00:00:00

  • Involvement of brassinosteroid signals in the floral-induction network of Arabidopsis.

    abstract::The transition to flowering is known to be regulated by numerous interacting endogenous and environmental cues, of which brassinosteroids (BRs), a group of polyhydroxylated steroid phytohormones, appear to be linked to the regulation of flowering time. In Arabidopsis, BR biosynthetic det2 mutants exhibited delayed flo...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章,评审

    doi:10.1093/jxb/erq241

    authors: Li J,Li Y,Chen S,An L

    更新日期:2010-10-01 00:00:00

  • The 2'-O-methyladenosine nucleoside modification gene OsTRM13 positively regulates salt stress tolerance in rice.

    abstract::Stress induces changes of modified nucleosides in tRNA, and these changes can influence codon-anticodon interaction and therefore the translation of target proteins. Certain nucleoside modification genes are associated with regulation of stress tolerance and immune response in plants. In this study, we found a dramati...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erx061

    authors: Wang Y,Li D,Gao J,Li X,Zhang R,Jin X,Hu Z,Zheng B,Persson S,Chen P

    更新日期:2017-03-01 00:00:00

  • A link between magnesium-chelatase H subunit and sucrose nonfermenting 1 (SNF1)-related protein kinase SnRK2.6/OST1 in Arabidopsis guard cell signalling in response to abscisic acid.

    abstract::Magnesium-chelatase H subunit [CHLH/putative abscisic acid (ABA) receptor ABAR] positively regulates guard cell signalling in response to ABA, but the molecular mechanism remains largely unknown. A member of the sucrose nonfermenting 1 (SNF1)-related protein kinase 2 family, SnRK2.6/open stomata 1 (OST1)/SRK2E, which ...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erv341

    authors: Liang S,Lu K,Wu Z,Jiang SC,Yu YT,Bi C,Xin Q,Wang XF,Zhang DP

    更新日期:2015-10-01 00:00:00

  • Cotton GhMKK5 affects disease resistance, induces HR-like cell death, and reduces the tolerance to salt and drought stress in transgenic Nicotiana benthamiana.

    abstract::Mitogen-activated protein kinase (MAPK) cascades are involved in various processes from plant growth and development to biotic and abiotic stress responses. MAPK kinases (MAPKKs), which link MAPKs and MAPKK kinases (MAPKKKs), play crucial roles in MAPK cascades to mediate a variety of stress responses in plants. Howev...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/ers086

    authors: Zhang L,Li Y,Lu W,Meng F,Wu CA,Guo X

    更新日期:2012-06-01 00:00:00

  • The impact of chromatin dynamics on plant light responses and circadian clock function.

    abstract::Research on the functional properties of nucleosome structure and composition dynamics has revealed that chromatin-level regulation is an essential component of light signalling and clock function in plants, two processes that rely extensively on transcriptional controls. In particular, several types of histone post-t...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章,评审

    doi:10.1093/jxb/eru011

    authors: Barneche F,Malapeira J,Mas P

    更新日期:2014-06-01 00:00:00

  • Natural variation in stomatal response to closing stimuli among Arabidopsis thaliana accessions after exposure to low VPD as a tool to recognize the mechanism of disturbed stomatal functioning.

    abstract::Stomatal responses to closing stimuli are disturbed after long-term exposure of plants to low vapour pressure deficit (VPD). The mechanism behind this disturbance is not fully understood. Genetic variation between naturally occurring ecotypes can be helpful to elucidate the mechanism controlling stomatal movements in ...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/eru370

    authors: Aliniaeifard S,van Meeteren U

    更新日期:2014-12-01 00:00:00

  • Expression of galactinol synthase from Ammopiptanthus nanus in tomato improves tolerance to cold stress.

    abstract::Soluble carbohydrates not only directly affect plant growth and development but also act as signal molecules in processes that enhance tolerance to cold stress. Raffinose family oligosaccharides (RFOs) are an example and play an important role in abiotic stress tolerance. This study aimed to determine whether galactin...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erz450

    authors: Liu Y,Zhang L,Meng S,Liu Y,Zhao X,Pang C,Zhang H,Xu T,He Y,Qi M,Li T

    更新日期:2020-01-01 00:00:00

  • Modified sucrose, starch, and ATP levels in two alloplasmic male-sterile lines of B. napus.

    abstract::Alloplasmic lines of Brassica napus with rearranged Arabidopsis thaliana mitochondrial DNA are male sterile and vegetatively altered compared with B. napus cv. Hanna. The CMS lines contain pure nuclear and plastid genomes from B. napus. Cross-sections of leaves revealed elevated starch accumulation and a higher number...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/eri120

    authors: Teixeira RT,Knorpp C,Glimelius K

    更新日期:2005-04-01 00:00:00

  • Using chromosome introgression lines to map quantitative trait loci for photosynthesis parameters in rice (Oryza sativa L.) leaves under drought and well-watered field conditions.

    abstract::Photosynthesis is fundamental to biomass production, but sensitive to drought. To understand the genetics of leaf photosynthesis, especially under drought, upland rice cv. Haogelao, lowland rice cv. Shennong265, and 94 of their introgression lines (ILs) were studied at flowering and grain filling under drought and wel...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/err292

    authors: Gu J,Yin X,Struik PC,Stomph TJ,Wang H

    更新日期:2012-01-01 00:00:00

  • Ionic and osmotic relations in quinoa (Chenopodium quinoa Willd.) plants grown at various salinity levels.

    abstract::Ionic and osmotic relations in quinoa (Chenopodium quinoa Willd.) were studied by exposing plants to six salinity levels (0-500 mM NaCl range) for 70 d. Salt stress was administered either by pre-mixing of the calculated amount of NaCl with the potting mix before seeds were planted or by the gradual increase of NaCl l...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erq257

    authors: Hariadi Y,Marandon K,Tian Y,Jacobsen SE,Shabala S

    更新日期:2011-01-01 00:00:00

  • Sustained enhancement of photosynthesis in coffee trees grown under free-air CO2 enrichment conditions: disentangling the contributions of stomatal, mesophyll, and biochemical limitations.

    abstract::Coffee (Coffea spp.), a globally traded commodity, is a slow-growing tropical tree species that displays an improved photosynthetic performance when grown under elevated atmospheric CO2 concentrations ([CO2]). To investigate the mechanisms underlying this response, two commercial coffee cultivars (Catuaí and Obatã) we...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erv463

    authors: DaMatta FM,Godoy AG,Menezes-Silva PE,Martins SC,Sanglard LM,Morais LE,Torre-Neto A,Ghini R

    更新日期:2016-01-01 00:00:00

  • A transcriptomic study of grapevine (Vitis vinifera cv. Cabernet-Sauvignon) interaction with the vascular ascomycete fungus Eutypa lata.

    abstract::Eutypa dieback is a vascular disease that may severely affect vineyards throughout the world. In the present work, microarrays were made in order (i) to improve our knowledge of grapevine (Vitis vinifera cv. Cabernet-Sauvignon) responses to Eutypa lata, the causal agent of Eutypa dieback; and (ii) to identify genes th...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erq040

    authors: Camps C,Kappel C,Lecomte P,Léon C,Gomès E,Coutos-Thévenot P,Delrot S

    更新日期:2010-06-01 00:00:00

  • Auxin regulation of Arabidopsis flower development involves members of the AINTEGUMENTA-LIKE/PLETHORA (AIL/PLT) family.

    abstract::Auxin is an important regulator of many aspects of plant growth and development. During reproductive development, auxin specifies the site of flower initiation and subsequently regulates organ growth and patterning as well as later events that determine reproductive success. Underlying auxin action in plant tissues is...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章,评审

    doi:10.1093/jxb/err127

    authors: Krizek BA

    更新日期:2011-06-01 00:00:00

  • The novel rice (Oryza sativa L.) gene OsSbf1 encodes a putative member of the Na+/bile acid symporter family.

    abstract::PCR-based differential screening was used to identify ethylene-induced genes in deep-water rice (Oryza sativa L.). One of the isolated cDNAs represented a novel protein, OsSBF1, with high homology to mammalian Na+/bile acid transporters and to sodium-dependent transporters from bacteria. One highly homologous protein ...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erf053

    authors: Rzewuski G,Sauter M

    更新日期:2002-09-01 00:00:00