Abstract：
:Voltage dependence of ionic currents and ion fluxes in a walled, turgor-regulating cell were measured in Neurospora crassa. The hyphal morphology of the model organism Neurospora simplifies cable analysis of ionic currents to determine current density for quantitative comparisons with ion fluxes. The ion fluxes were measured directly and non-invasively with self-referencing ion-selective microelectrodes. Four ions (H(+), Ca(2+), K(+), and Cl(-)) were examined. H(+) net uptake and Ca(2+) net release were small (10.2 nmol m(-2) s(-1) and 1.1 nmol m(-2) s(-1), respectively) and voltage independent. K(+) and Cl(-) fluxes were larger and voltage dependent. Maximal K(+) net release ( approximately 1440 nmol m(-2) s(-1)) was observed at positive voltages (+15 mV), while maximal Cl(-) net release ( approximately 905 nmol m(-2) s(-1)) was observed at negative voltage (-210 mV). A possible function of the net outward K(+) and Cl(-) fluxes is regulation of the plasma membrane potential. Total ion fluxes were 37-58% of the total ionic current density (about +/-244 mA m(-2), equivalent to +/-2500 nmol m(-2) s(-1), at 0 mV and -200 mV) so other ions must contribute significantly to the ionic currents.
journal_name
J Exp Botjournal_title
Journal of experimental botanyauthors
Lew RRdoi
10.1093/jxb/erm204subject
Has Abstractpub_date
2007-01-01 00:00:00pages
3475-81issue
12eissn
0022-0957issn
1460-2431pii
erm204journal_volume
58pub_type
杂志文章abstract：:We initially observed that Brassica rapa cultivars containing the Turnip mosaic virus (TuMV) resistance gene, Rnt1-1, accumulated a high level of endogenous ascorbic acid (AS) and dehydroascobic acid (DHA) when infected with TuMV. We here hypothesized a possible contribution of an elevated level of AS+DHA (TAA) to the...
journal_title：Journal of experimental botany
pub_type： 杂志文章
doi：10.1093/jxb/erw223
更新日期：2016-07-01 00:00:00
abstract：:An Arabidopsis thaliana cDNA (At-74) has been isolated that encoded an uncharacterized protein showing homology with members of the d-PGMase superfamily: cofactor-dependent phosphoglycerate mutases (d-PGM-ases) and the phosphatase domain of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatases (6PF2Kase/F2, 6Pase). Pr...
journal_title：Journal of experimental botany
pub_type： 杂志文章
doi：10.1093/jxb/eri105
更新日期：2005-04-01 00:00:00
abstract：:Hydration at low temperatures, commonly referred to as cold stratification, is widely used for releasing dormancy and triggering germination in a wide range of species including wheat. However, the molecular mechanism that underlies its effect on germination has largely remained unknown. Our previous studies showed th...
journal_title：Journal of experimental botany
pub_type： 杂志文章
doi：10.1093/jxb/erw172
更新日期：2016-05-01 00:00:00
abstract：:Many molecular mechanisms that regulate dormancy have been identified individually in controlled laboratory studies. However, little is known about how the seed employs this complex suite of mechanisms during dormancy cycling in the variable environment of the soil seed bank. Nevertheless, this behaviour is essential ...
journal_title：Journal of experimental botany
pub_type： 杂志文章,评审
doi：10.1093/jxb/erw477
更新日期：2017-02-01 00:00:00
abstract：:Short-chain esters contribute to the blend of volatiles that define the strawberry aroma. The last step in their biosynthesis involves an alcohol acyltransferase that catalyses the esterification of an acyl moiety of acyl-CoA with an alcohol. This study identified a novel strawberry alcohol acyltransferase gene (FaAAT...
journal_title：Journal of experimental botany
pub_type： 杂志文章
doi：10.1093/jxb/ers120
更新日期：2012-06-01 00:00:00
abstract：:The feedback regulation of ethylene biosynthesis in banana [Musa sp. (AAA group, Cavendish subgroup) cv. Grand Nain] fruit was investigated in an attempt to clarify the opposite effect of 1-methylcyclopropene (1-MCP), an ethylene action inhibitor, before and after the onset of ripening. 1-MCP pre-treatment completely ...
journal_title：Journal of experimental botany
pub_type： 杂志文章
doi：10.1093/jxb/erl265
更新日期：2007-01-01 00:00:00
abstract：:Mitochondria from plants, yeast, and animals each contain at least one peroxiredoxin (Prx) that is involved in peroxide detoxification and redox signalling. The supramolecular dynamics of atypical type II Prx targeted to the mitochondrion was addressed in pea. Microcalorimetric (ITC) titrations identified an extremely...
journal_title：Journal of experimental botany
pub_type： 杂志文章
doi：10.1093/jxb/ern177
更新日期：2008-01-01 00:00:00
abstract：:Plant nucleotide-binding domain, leucine-rich repeat receptor (NLR) proteins play important roles in recognition of pathogen-derived effectors. However, the mechanism by which plant NLRs activate immunity is still largely unknown. The paired Arabidopsis NLRs RRS1-R and RPS4, that confer recognition of bacterial effect...
journal_title：Journal of experimental botany
pub_type： 杂志文章
doi：10.1093/jxb/erz571
更新日期：2020-03-25 00:00:00
abstract：:The eukaryotic actin cytoskeleton is a highly dynamic framework that is involved in many biological processes, such as cell growth, division, morphology, and motility. G-actin polymerizes into microfilaments that associate into bundles, patches, and networks, which, in turn, organize into higher order structures that ...
journal_title：Journal of experimental botany
pub_type： 杂志文章,评审
doi：10.1093/jxb/erx195
更新日期：2017-06-15 00:00:00
abstract：:Receptor kinases play important roles in plant growth and development, but only few of them have been functionally characterized in depth. Over the past decade CRINKLY 4 (CR4)-related research has peaked as a result of a newly discovered role of ARABIDOPSIS CR4 (ACR4) in the root. Here, we comprehensively review the a...
journal_title：Journal of experimental botany
pub_type： 杂志文章,评审
doi：10.1093/jxb/erw192
更新日期：2016-08-01 00:00:00
abstract：:Since cysteine is the first committed molecule in plant metabolism containing both sulphur and nitrogen, the regulation of its biosynthesis is critically important. Cysteine itself is required for the production of an abundance of key metabolites in diverse pathways. Plants alter their metabolism to compensate for sul...
journal_title：Journal of experimental botany
pub_type： 杂志文章,评审
doi：10.1093/jxb/erh136
更新日期：2004-06-01 00:00:00
abstract：:Brassinosteroids (BRs) are essential for many biological processes in plants, however, little is known about their roles in early fruit development. To address this, BR levels were manipulated through the application of exogenous BRs (24-epibrassinolide, EBR) or a BR biosynthesis inhibitor (brassinazole, Brz) and thei...
journal_title：Journal of experimental botany
pub_type： 杂志文章
doi：10.1093/jxb/ern093
更新日期：2008-01-01 00:00:00
abstract：:Soil fertility provides the foundation for nutritious food production and resilient and sustainable livelihoods. A comprehensive survey and summit meeting were conducted with the aims of understanding barriers to enhancing soil fertility in sub-Saharan Africa and providing evidence-based recommendations. The focus reg...
journal_title：Journal of experimental botany
pub_type： 杂志文章
doi：10.1093/jxb/erz446
更新日期：2020-01-07 00:00:00
abstract：:Mobilization of reserves in germinated cereal grains is critical for early seedling vigour, global crop productivity, and hence food security. Gibberellins (GAs) are central to this process. We have developed a spatio-temporal model that describes the multifaceted mechanisms of GA regulation in germinated barley grain...
journal_title：Journal of experimental botany
pub_type： 杂志文章
doi：10.1093/jxb/erz546
更新日期：2020-03-25 00:00:00
abstract：:Examination of root tips from cucumber (Cucumis sativus L.) seedlings grown at 8 degrees C for varying periods ranging from 15 min to 96 h, showed marked changes in the ultrastructure of cortical cells within only 15 min of exposure. Greater parts of the cortex were affected with longer periods of exposure, but the se...
journal_title：Journal of experimental botany
pub_type： 杂志文章
doi：10.1093/jxb/erf071
更新日期：2002-11-01 00:00:00
abstract：:Low-temperature stress is an important factor affecting the growth and development of rice (Oryza sativa L.) in temperate and high-elevation areas. Cold stress may cause various seedling injuries, delayed heading and yield reduction due to spikelet sterility. In this study, 181 microsatellite marker loci were used to ...
journal_title：Journal of experimental botany
pub_type： 杂志文章
doi：10.1093/jxb/erg243
更新日期：2003-11-01 00:00:00
abstract：:Successful phloem feeding requires overcoming a number of phloem-related plant properties and reactions. The most important hurdle is formed by the phloem wound responses, such as coagulating proteins in the phloem sieve elements of the plant and in the capillary food canal in the insect's mouth parts, i.e. the stylet...
journal_title：Journal of experimental botany
pub_type： 杂志文章,评审
doi：10.1093/jxb/erj088
更新日期：2006-01-01 00:00:00
abstract：:The response of plants to the stress caused by herbivores involves several different defence mechanisms. These responses begin at the plant cell plasma membrane, where insect herbivores interact physically by causing mechanical damage and chemically by introducing elicitors or by triggering plant-derived signalling mo...
journal_title：Journal of experimental botany
pub_type： 杂志文章,评审
doi：10.1093/jxb/eru480
更新日期：2015-02-01 00:00:00
abstract：:Two groups of linear gene constructs (gus and bar, and 1Ax1 and bar) lacking vector backbone sequences were independently transferred into the elite wheat (Triticum aestivum L.) variety EM12, and genetically stable transgenic plants with low copy number transgene integration were recovered. Co-transformation experimen...
journal_title：Journal of experimental botany
pub_type： 杂志文章
doi：10.1093/jxb/erl145
更新日期：2006-01-01 00:00:00
abstract：:Water deficits are known to alter fine root structure and function, but little is known about how these responses contribute to differences in drought resistance across grapevine rootstocks. The ways in which water deficit affects root anatomical and physiological characteristics were studied in two grapevine rootstoc...
journal_title：Journal of experimental botany
pub_type： 杂志文章
doi：10.1093/jxb/erv324
更新日期：2015-09-01 00:00:00
abstract：:Inside chloroplasts, several abiotic stresses (including drought, high light, salinity, or extreme temperatures) induce a reduction in CO2 assimilation rates with a consequent increase in reactive oxygen species (ROS) production, ultimately leading to leaf senescence and yield loss. Photo-oxidation processes should th...
journal_title：Journal of experimental botany
pub_type： 杂志文章,评审
doi：10.1093/jxb/eru086
更新日期：2014-07-01 00:00:00
abstract：:The structure of arabinan and galactan domains in association with cellulose microfibrils was investigated using enzymatic and alkali degradation procedures. Sugar beet and potato cell wall residues (called 'natural' composites), rich in pectic neutral sugar side chains and cellulose, as well as 'artificial' composite...
journal_title：Journal of experimental botany
pub_type： 杂志文章
doi：10.1093/jxb/erm037
更新日期：2007-01-01 00:00:00
abstract：:Plants have fundamental dependences on nitrogen and sulfur and frequently have to cope with chronic limitations when their supply is sub-optimal. This study aimed at characterizing the metabolomic, proteomic, and transcriptomic changes occurring in Arabidopsis leaves under chronic nitrate (Low-N) and chronic sulfate (...
journal_title：Journal of experimental botany
pub_type： 杂志文章
doi：10.1093/jxb/eraa337
更新日期：2020-10-22 00:00:00
abstract：:Oleoresin produced and stored in pine tree leaves provides direct resistance to herbivores, while leaf volatile terpenes (LVT) in the resin are also powerful airborne infochemicals. Resin concentration and profile show considerable spatial and temporal phenotypic variation within and among pine populations. LVT bioche...
journal_title：Journal of experimental botany
pub_type： 杂志文章
doi：10.1093/jxb/erq246
更新日期：2010-10-01 00:00:00
abstract：:In Crocus vernus, a spring bulbous species, prolonged growth at low temperatures results in the development of larger perennial organs and delayed foliar senescence. Because corm growth is known to stop before the first visual sign of leaf senescence, it is clear that factors other than leaf duration alone determine f...
journal_title：Journal of experimental botany
pub_type： 杂志文章
doi：10.1093/jxb/erp103
更新日期：2009-01-01 00:00:00
abstract：:Polyacetylene (PA) compounds from Bidens pilosa are known to have several pharmacological activities. In this study, we aimed to elucidate the major genes encoding enzymes involved in the biosynthesis of PA in B. pilosa. Seven PA metabolites present in B. pilosa leaves were found to be induced by methyl jasmonate (MeJ...
journal_title：Journal of experimental botany
pub_type： 杂志文章
doi：10.1093/jxb/eraa457
更新日期：2020-10-16 00:00:00
abstract：:Increasing drought stress poses a severe threat to agricultural productivity. Plants, however, evolved numerous mechanisms to cope with such environmental stress. Here we report that the stress-induced production of a peptide signal contributes to stress tolerance. The expression of phytosulfokine (PSK) peptide precur...
journal_title：Journal of experimental botany
pub_type： 杂志文章
doi：10.1093/jxb/erab017
更新日期：2021-01-20 00:00:00
abstract：:The stamen produces pollen grains for pollination in higher plants. Coordinated development of four microsporangia in the stamen is essential for normal fertility. The roles of miR165/166-directed pathways in the establishment of adaxial-abaxial polarity have been well defined in leaves. However, the molecular mechani...
journal_title：Journal of experimental botany
pub_type： 杂志文章
doi：10.1093/jxb/ert178
更新日期：2013-08-01 00:00:00
abstract：:Teosinte branched1/cycloidea/proliferating (TCP) transcription factors play a broad role in plant growth and development, but their involvement in the regulation of anthocyanin biosynthesis is currently unclear. In this study, anthocyanin biosynthesis induced by different light intensities in apple (Malus domestica) w...
journal_title：Journal of experimental botany
pub_type： 杂志文章
doi：10.1093/jxb/eraa056
更新日期：2020-05-30 00:00:00
abstract：:A recent analysis of the hexokinase (HXK) gene family from Arabidopsis revealed that three hexokinase-like (HKL) proteins lack catalytic activity, but share about 50% identity with the primary glucose (glc) sensor/transducer protein AtHXK1. Since the AtHKL1 protein is predicted to bind glc, although with a relatively ...
journal_title：Journal of experimental botany
pub_type： 杂志文章
doi：10.1093/jxb/erp252
更新日期：2009-01-01 00:00:00
