Abstract:
:The stamen produces pollen grains for pollination in higher plants. Coordinated development of four microsporangia in the stamen is essential for normal fertility. The roles of miR165/166-directed pathways in the establishment of adaxial-abaxial polarity have been well defined in leaves. However, the molecular mechanism underlying the adaxial-abaxial polarity of the stamen is elusive. Here it is reported that HYPONASTIC LEAVES1 (HYL1), a general regulator of microRNA (miRNA) biogenesis, plays an essential role in establishing the stamen architecture of the four microsporangia in Arabidopsis thaliana. In stamens, HYL1 and miR165/6 expression are progressively restricted to the lateral region, microsporangia, microspore mother cells, and microspores, whereas HD-ZIP III genes are preferentially expressed in the middle region, vascular bundle, and stomium. Loss of HYL1 leads to the formation of two rather than four microsporangia in each stamen. In the stamen of the hyl1 mutant, miR165/6 accumulation is reduced, whereas miR165/6-targeted HD-ZIP III genes are up-regulated and FILAMENTOUS FLOWER (FIL) is down-regulated; and, specifically, REVOLUTA (REV) is overexpressed in the adaxial region and FIL is underexpressed in the abaxial regions, concomitant with the aberrance of the two inner microsporangia and partial adaxialization of the connectives. Genetic analysis reveals that FIL works downstream of HYL1, and the defects in hyl1 stamens are partially rescued by rev-9 or phv-5 phb-6 alleles. These results suggest that HYL1 modulates inner microsporangia and stamen architecture by repression of HD-ZIP III genes and promotion of the FIL gene through miR165/6. Thus, the role of HYL1 in establishment of stamen architecture provides insight into the molecular mechanism of male fertility.
journal_name
J Exp Botjournal_title
Journal of experimental botanyauthors
Lian H,Li X,Liu Z,He Ydoi
10.1093/jxb/ert178subject
Has Abstractpub_date
2013-08-01 00:00:00pages
3397-410issue
11eissn
0022-0957issn
1460-2431pii
ert178journal_volume
64pub_type
杂志文章abstract::Cortical microtubules (CMTs) are often aligned in a particular direction in individual cells or even in groups of cells and play a central role in the definition of growth anisotropy. How the CMTs themselves are aligned is not well known, but two hypotheses have been proposed. According to the first hypothesis, CMTs a...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/ert352
更新日期:2013-12-01 00:00:00
abstract::Plant programmed cell death (PCD) is a genetically controlled process that plays an important role in development and stress responses. Reactive oxygen species (ROS) are key inducers of PCD. The addition of 50 mM H₂O₂ to tobacco Bright Yellow-2 (TBY-2) cell cultures induces PCD. A comparative proteomic analysis of TBY...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/ers031
更新日期:2012-05-01 00:00:00
abstract::A cDNA sequence coding for a pea (Pisum sativum L.) 2-Cys peroxiredoxin (2-Cys Prx) has been cloned. The deduced amino acid sequence showed a high sequence homology to the 2-Cys Prx enzymes of Phaseolus vulgaris (86%), Arabidopsis thaliana (75%), and Spinacia oleracea (75%), and contained a chloroplast target sequence...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erh238
更新日期:2004-10-01 00:00:00
abstract::Leaf senescence is a very important trait that limits yield and biomass accumulation of agronomic crops and reduces post-harvest performance and the nutritional value of horticultural crops. Significant advance in physiological and molecular understanding of leaf senescence has made it possible to devise ways of manip...
journal_title:Journal of experimental botany
pub_type: 杂志文章,评审
doi:10.1093/jxb/eru248
更新日期:2014-07-01 00:00:00
abstract::Two unlinked semi-dominant loci, A (NIC1) and B (NIC2), control nicotine and related alkaloid biosynthesis in Burley tobaccos. Mutations in either or both loci (nic1 and nic2) lead to low nicotine phenotypes with altered environmental stress responses. Here we show that the transcripts derived from the pathogenesis-re...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erw345
更新日期:2016-10-01 00:00:00
abstract::Mango is characterized by high tocopherol and carotenoid content during ripening. From a cDNA screen of differentially expressing genes during mango ripening, a full-length p-hydroxyphenylpyruvate dioxygenase (MiHPPD) gene homologue was isolated that encodes a key enzyme in the biosynthesis of tocopherols. The gene en...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/err006
更新日期:2011-06-01 00:00:00
abstract::The plant root system is important for the uptake of water and nutrients and the anchoring of plants in the soil. Lateral roots (LRs) contribute considerably to root system architecture. Their post-embryonic formation is regulated by hormones and environmental cues. The hormone cytokinin influences LR formation and gr...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/ert291
更新日期:2013-11-01 00:00:00
abstract::This paper outlines a numerical scheme for accurate, detailed, and high-throughput image analysis of plant roots. In contrast to existing root image analysis tools that focus on root system-average traits, a novel, fully automated and robust approach for the detailed characterization of root traits, based on a graph o...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erv359
更新日期:2015-11-01 00:00:00
abstract::One of the earliest symptoms of boron (B) deficiency is the inhibition of root elongation which can reasonably be attributed to the damaging effects of B deprivation on cell wall integrity. It is shown here that exposure of wild-type Arabidopsis thaliana seedlings to B deficiency for 4h led to a drastic inhibition of ...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erv186
更新日期:2015-07-01 00:00:00
abstract::Storage of phosphorus (P) in stem tissue is important in Mediterranean Proteaceae, because proteoid root growth and P uptake is greatest during winter, whereas shoot growth occurs mostly in summer. This has prompted the present investigation of the P distribution amongst roots, stems, and leaves of Hakea prostrata R.B...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erh111
更新日期:2004-05-01 00:00:00
abstract::Virus-induced flowering (VIF) exploits RNA or DNA viruses to express flowering time genes to induce flowering in plants. Such plant virus-based tools have recently attracted widespread attention for their fundamental and applied uses in flowering physiology and in accelerating breeding in dicotyledonous crops and wood...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/eraa080
更新日期:2020-05-30 00:00:00
abstract::The response of both specific (ascorbate peroxidase, APX) and unspecific (POD) peroxidases and H(2)O(2) content of sunflower plants (Helianthus annuus L. cv. Hor) grown hydroponically with (C) or without (-Fe) iron in the nutrient solution were analysed to verify whether iron deficiency led to cell oxidative status. I...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:
更新日期:2001-01-01 00:00:00
abstract::Leaf dry mass per area (LMA) is a composite parameter relating to a suite of structural traits that have the potential to influence photosynthesis. However, the extent to which each of these traits contributes to variation in LMA and photosynthetic rates is not well understood, especially at the high end of the LMA sp...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erq128
更新日期:2010-06-01 00:00:00
abstract::In order to understand the role of cytosolic antioxidant enzymes in drought stress protection, transgenic tobacco (Nicotiana tabacum cv. Xanthi) plants overexpressing cytosolic Cu/Zn-superoxide dismutase (cytsod) (EC 1.15.1.1) or ascorbate peroxidase (cytapx) (EC 1.11.1.1) alone, or in combination, were produced and t...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erq432
更新日期:2011-05-01 00:00:00
abstract::A gene encoding a RING zinc finger ankyrin repeat protein (MjXB3), a putative E3 ubiquitin ligase, is highly expressed in petals of senescing four o'clock (Mirabilis jalapa) flowers, increasing >40,000-fold during the onset of visible senescence. The gene has homologues in many other species, and the Petunia homologue...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erm212
更新日期:2007-01-01 00:00:00
abstract::Abscisic acid conjugate concentrations increased in barley xylem sap under salinity, whereas it remained at a low level in the intercellular washing fluid (IWF) of barley primary leaves (Hordeum vulgare cv. Gerbel). Here it is shown that IWF contains beta-glucosidase activity which releases abscisic acid (ABA) from th...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:
更新日期:2000-05-01 00:00:00
abstract::Groundnuts (Arachis hypogaea L.) are an important crop of the semi-arid tropics where they are often exposed to maximum temperatures of > 40 degrees C for short periods during the growing season. The objectives of this study were to determine: (i) the effects of short periods of exposure to high temperature on flower ...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jexbot/51.345.777
更新日期:2000-04-01 00:00:00
abstract::Soybean (Glycine max L. Merr.) plants exposed to 10 mM KNO(3) for a 4 d period were used to test the correlation between nitrogenase activity, gene expression and sucrose metabolism. Nitrate caused the down-regulation of sucrose synthase (SS) transcripts within 1 d, although a decline in nodule SS activity and an incr...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jexbot/53.368.423
更新日期:2002-03-01 00:00:00
abstract::Sugars play an important role in grapevine flowering. This complex process from inflorescence initiation to fruit maturity takes two growing seasons. Currently, most of the available data concern the involvement of sugars as energy sources during the formation of reproductive structures from initiation of inflorescenc...
journal_title:Journal of experimental botany
pub_type: 杂志文章,评审
doi:10.1093/jxb/ern135
更新日期:2008-01-01 00:00:00
abstract::Seed loss resulting from pod shattering is a major constraint in production of oilseed rape (Brassica napus L.). However, the molecular mechanisms underlying pod shatter resistance are not well understood. Here, we show that the pod shatter resistance at quantitative trait locus qSRI.A9.1 is controlled by one of the B...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/eraa281
更新日期:2020-09-19 00:00:00
abstract::Soil fertility provides the foundation for nutritious food production and resilient and sustainable livelihoods. A comprehensive survey and summit meeting were conducted with the aims of understanding barriers to enhancing soil fertility in sub-Saharan Africa and providing evidence-based recommendations. The focus reg...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erz446
更新日期:2020-01-07 00:00:00
abstract::The genetic basis of stem cell specification in somatic embryogenesis and organogenesis is still obscure. SOMATIC EMBRYOGENESIS RECEPTOR-LIKE KINASE (SERK) genes are involved in embryogenesis and organogenesis in numerous species. In vitro culture of Cyclamen persicum immature ovules provides a system for investigatin...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/err295
更新日期:2012-01-01 00:00:00
abstract::Most plant oxylipins, a large class of diverse oxygenated polyunsaturated fatty acids and their derivatives, are produced through the lipoxygenase (LOX) pathway. Recent progress in dicots has highlighted the biological roles of oxylipins in plant defence responses to pathogens and pests. By contrast, the physiological...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erl137
更新日期:2006-01-01 00:00:00
abstract::An alkaline proteinase activity is present in quiescent seeds and up to the 24th day of development of Canavalia ensiformis DC (L.) plants. By a simple protocol consisting of cation exchange chromatography, followed by an anion exchange column, a serine proteinase (Q-SP) was purified to homogeneity from quiescent seed...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erl223
更新日期:2007-01-01 00:00:00
abstract::Cytoplasmic male sterility (CMS) is a widespread phenomenon in flowering plants caused by mitochondrial (mt) genes. CMS genes typically encode novel proteins that interfere with mt functions and can be silenced by nuclear fertility-restorer genes. Although the molecular basis of CMS is well established in a number of ...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erx057
更新日期:2017-03-01 00:00:00
abstract::In eukaryotes, the RHO superfamily of small G-proteins is implicated in the regulation of cell polarity and growth. Rho of Plants (ROPs)/RACs are plant-specific Rho family proteins that have been shown to regulate cell polarity, auxin transport and responses, ABA signalling, and response to pathogens. A hallmark of RO...
journal_title:Journal of experimental botany
pub_type: 杂志文章,评审
doi:10.1093/jxb/erv057
更新日期:2015-03-01 00:00:00
abstract::Rice is an important crop that is very sensitive to salinity. However, some varieties differ greatly in this feature, making investigations of salinity tolerance mechanisms possible. The cultivar Pokkali is salinity tolerant and is known to have more extensive hydrophobic barriers in its roots than does IR20, a more s...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/err135
更新日期:2011-08-01 00:00:00
abstract::We previously reported that a novel motor protein belonging to the kinesin-12 family, NtKRP, displays critical roles in regulating embryo and seed size establishment. However, it remains unknown exactly how NtKRP contributes to this developmental process. Here, we report that a 60S ribosomal protein NtRPL17 directly i...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erx361
更新日期:2017-11-28 00:00:00
abstract::Non-invasive X-ray computed tomography (XRCT) is increasingly used in rhizosphere research to visualize development of soil-root interfaces in situ. However, exposing living systems to X-rays can potentially impact their processes and metabolites. In order to evaluate these effects, we assessed the responses of rhizos...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/eraa262
更新日期:2020-09-19 00:00:00
abstract::C-to-U RNA editing in plant mitochondria requires the participation of many nucleus-encoded factors, most of which are pentatricopeptide repeat (PPR) proteins. There is a large number of PPR proteins and the functions many of them are unknown. Here, we report a mitochondrion-localized DYW-subgroup PPR protein, PPR27, ...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/eraa273
更新日期:2020-09-19 00:00:00