Non-coding RNA may be associated with cytoplasmic male sterility in Silene vulgaris.

Abstract:

:Cytoplasmic male sterility (CMS) is a widespread phenomenon in flowering plants caused by mitochondrial (mt) genes. CMS genes typically encode novel proteins that interfere with mt functions and can be silenced by nuclear fertility-restorer genes. Although the molecular basis of CMS is well established in a number of crop systems, our understanding of it in natural populations is far more limited. To identify CMS genes in a gynodioecious plant, Silene vulgaris, we constructed mt transcriptomes and compared transcript levels and RNA editing patterns in floral bud tissue from female and hermaphrodite full siblings. The transcriptomes from female and hermaphrodite individuals were very similar overall with respect to variation in levels of transcript abundance across the genome, the extent of RNA editing, and the order in which RNA editing and intron splicing events occurred. We found only a single genomic region that was highly overexpressed and differentially edited in females relative to hermaphrodites. This region is not located near any other transcribed elements and lacks an open-reading frame (ORF) of even moderate size. To our knowledge, this transcript would represent the first non-coding mt RNA associated with CMS in plants and is, therefore, an important target for future functional validation studies.

journal_name

J Exp Bot

authors

Stone JD,Koloušková P,Sloan DB,Štorchová H

doi

10.1093/jxb/erx057

subject

Has Abstract

pub_date

2017-03-01 00:00:00

pages

1599-1612

issue

7

eissn

0022-0957

issn

1460-2431

pii

3092367

journal_volume

68

pub_type

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