Abstract:
:Environmental stresses such as drought, heat, and salinity limit plant development and agricultural productivity. While individual stresses have been studied extensively, much less is known about the molecular interaction of responses to multiple stresses. To address this problem, we investigated molecular responses of Arabidopsis to single, double, and triple combinations of salt, osmotic, and heat stresses. A metabolite profiling analysis indicated the production of specific compatible solutes depending on the nature of the stress applied. We found that in combination with other stresses, heat has a dominant effect on global gene expression and metabolite level patterns. Treatments that include heat stress lead to strongly reduced transcription of genes coding for abundant photosynthetic proteins and proteins regulating the cell life cycle, while genes involved in protein degradation are up-regulated. Under combined stress conditions, the plants shifted their metabolism to a survival state characterized by low productivity. Our work provides molecular evidence for the dangers for plant productivity and future world food security posed by heat waves resulting from global warming. We highlight candidate genes, many of which are functionally uncharacterized, for engineering plant abiotic stress tolerance.
journal_name
J Exp Botjournal_title
Journal of experimental botanyauthors
Sewelam N,Brilhaus D,Bräutigam A,Alseekh S,Fernie AR,Maurino VGdoi
10.1093/jxb/eraa250subject
Has Abstractpub_date
2020-08-06 00:00:00pages
5098-5112issue
16eissn
0022-0957issn
1460-2431pii
5842162journal_volume
71pub_type
杂志文章abstract::With the aim of increasing the cysteine level in rice (Oryza sativa L.) and thus improving its nutritional quality, transgenic rice plants were generated expressing an Escherichia coli serine acetyltransferase isoform (EcSAT), the enzyme synthesizing O-acetylserine, the precursor of cysteine. The gene was fused to the...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/ers253
更新日期:2012-10-01 00:00:00
abstract::Previously, it was found that Nep1Mo (a Nep1-like protein from Magnaporthe oryzae) could trigger a variety of plant responses, including stomatal closure, hypersensitive cell death (HCD), and defence-related gene expression, in Nicotiana benthamiana. In this study, it was found that Nep1Mo-induced cell death could be ...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/eru136
更新日期:2014-06-01 00:00:00
abstract::Metabolic and physiological analyses of Arabidopsis thaliana glycolate oxidase (GOX) mutant leaves were performed to understand the development of the photorespiratory phenotype after transfer from high CO2 to air. We show that two Arabidopsis genes, GOX1 and GOX2, share a redundant photorespiratory role. Air-grown si...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erw054
更新日期:2016-05-01 00:00:00
abstract::The P1B ATPase heavy metal ATPase 4 (HMA4) is responsible for zinc and cadmium translocation from roots to shoots in Arabidopsis thaliana. It couples ATP hydrolysis to cytosolic domain movements, enabling metal transport across the membrane. The detailed mechanism of metal permeation by HMA4 through the membrane remai...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/ery353
更新日期:2019-01-01 00:00:00
abstract::Plant programmed cell death (PCD) is a genetically controlled process that plays an important role in development and stress responses. Reactive oxygen species (ROS) are key inducers of PCD. The addition of 50 mM H₂O₂ to tobacco Bright Yellow-2 (TBY-2) cell cultures induces PCD. A comparative proteomic analysis of TBY...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/ers031
更新日期:2012-05-01 00:00:00
abstract::The fact that silicon application alleviates water deficit stress has been widely reported, but the underlying mechanism remains unclear. Here the effects of silicon on water uptake and transport of sorghum seedlings (Sorghum bicolor L.) growing under polyethylene glycol-simulated osmotic stress in hydroponic culture ...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/eru220
更新日期:2014-09-01 00:00:00
abstract::Most terrestrial plants use C3 photosynthesis to fix carbon. In multiple plant lineages a modified system known as C4 photosynthesis has evolved. To better understand the molecular patterns associated with induction of C4 photosynthesis, the genus Flaveria that contains C3 and C4 species was used. A base to tip matura...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erw488
更新日期:2017-01-01 00:00:00
abstract::A cDNA that encodes a transporter from the NHAD family was identified in Physcomitrella patens. Computer-based searches using the amino acid sequence of PpNHAD1 revealed that, in addition to being expressed in flowering plants, highly conserved transporters of this family are expressed in red algae, green algae, mosse...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erm094
更新日期:2007-01-01 00:00:00
abstract::Cytokinin hormones are important regulators of development and environmental responses of plants that execute their action via the molecular machinery of signal perception and transduction. The limiting step of the whole process is the availability of the hormone in suitable concentrations in the right place and at th...
journal_title:Journal of experimental botany
pub_type: 杂志文章,评审
doi:10.1093/jxb/err004
更新日期:2011-05-01 00:00:00
abstract::Virus-induced gene silencing (VIGS) is a useful tool for functional characterization of genes in plants. Unfortunately, the efficiency of infection by Tobacco rattle virus (TRV) is relatively low for some non-Solanaceae plants, which are economically important, such as rose (Rosa sp.). Here, to generate an easy tracea...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/ert381
更新日期:2014-01-01 00:00:00
abstract::Natural vegetation is predicted to suffer from extreme heat events as a result of global warming. In this study, we focused on the immediate response to heat stress. Photosynthesis and volatile emissions were measured in the leaves of tobacco (Nicotiana tabacum cv. Wisconsin 38) after exposure to heat shock treatments...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erz255
更新日期:2019-09-24 00:00:00
abstract::The tomato (Solanum lycopersicum) sulfurea mutation displays trans-inactivation of wild-type alleles in heterozygous plants, a phenomenon referred to as paramutation. Homozygous mutant plants and paramutated leaf tissue of heterozygous plants show a pigment-deficient phenotype. The molecular basis of this phenotype an...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/ern213
更新日期:2008-01-01 00:00:00
abstract::Notwithstanding the economic importance of non-climacteric fruits like grape and strawberry, little is known about the mechanisms that regulate their ripening. Up to now no growth regulator has emerged with a primary role similar to that played by ethylene in the ripening of the climacteric fruits. Strawberries can pr...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/eri202
更新日期:2005-08-01 00:00:00
abstract::In order to understand the role of cytosolic antioxidant enzymes in drought stress protection, transgenic tobacco (Nicotiana tabacum cv. Xanthi) plants overexpressing cytosolic Cu/Zn-superoxide dismutase (cytsod) (EC 1.15.1.1) or ascorbate peroxidase (cytapx) (EC 1.11.1.1) alone, or in combination, were produced and t...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erq432
更新日期:2011-05-01 00:00:00
abstract::Relatively little is known about programmed cell death (PCD) in plants. It is nonetheless suggested here that tonoplast rupture and the subsequent rapid destruction of the cytoplasm can distinguish two large PCD classes. One class, which is here called 'autolytic', shows this feature, whilst the second class (called '...
journal_title:Journal of experimental botany
pub_type: 杂志文章,评审
doi:10.1093/jxb/err196
更新日期:2011-10-01 00:00:00
abstract::Understanding the evolution events defining silicon (Si) uptake in plant species is important for the efficient exploration of Si-derived benefits. In the present study, Si accumulation was studied in 456 diverse plant species grown in uniform field conditions, and in a subset of 151 species grown under greenhouse con...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/eraa342
更新日期:2020-12-02 00:00:00
abstract::While many C4 lineages have Kranz anatomy around individual veins, Salsoleae have evolved the Salsoloid Kranz anatomy where a continuous dual layer of chlorenchyma cells encloses the vascular and water-storage tissue. With the aim of elucidating the evolution of C4 photosynthesis in Salsoleae, a broadly sampled molecu...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erw432
更新日期:2017-01-01 00:00:00
abstract::Water deficits are known to alter fine root structure and function, but little is known about how these responses contribute to differences in drought resistance across grapevine rootstocks. The ways in which water deficit affects root anatomical and physiological characteristics were studied in two grapevine rootstoc...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erv324
更新日期:2015-09-01 00:00:00
abstract::Plant annexins are ubiquitous, soluble proteins capable of Ca(2+)-dependent and Ca(2+)-independent binding to endomembranes and the plasma membrane. Some members of this multigene family are capable of binding to F-actin, hydrolysing ATP and GTP, acting as peroxidases or cation channels. These multifunctional proteins...
journal_title:Journal of experimental botany
pub_type: 杂志文章,评审
doi:10.1093/jxb/erm344
更新日期:2008-01-01 00:00:00
abstract::Seed loss resulting from pod shattering is a major constraint in production of oilseed rape (Brassica napus L.). However, the molecular mechanisms underlying pod shatter resistance are not well understood. Here, we show that the pod shatter resistance at quantitative trait locus qSRI.A9.1 is controlled by one of the B...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/eraa281
更新日期:2020-09-19 00:00:00
abstract::By using a fine oil-filled glass microcapillary mounted on a micromanipulator, the solutes of individual plant cells can be sampled. These samples can then be analysed using a range of physical and chemical methods. Hydrostatic pressure (cell pressure probe), osmotic pressure (picolitre osmometer), organic solutes (en...
journal_title:Journal of experimental botany
pub_type: 杂志文章,评审
doi:
更新日期:2001-04-01 00:00:00
abstract::Portulacaceae is one of 19 families of terrestrial plants in which species having C(4) photosynthesis have been found. Representative species from major clades of the genus Portulaca were studied to characterize the forms of photosynthesis structurally and biochemically. The species P. amilis, P. grandiflora, P. molok...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erq178
更新日期:2010-08-01 00:00:00
abstract::Shoot branching is the process by which axillary buds, located on the axil of a leaf, develop and form new flowers or branches. The process by which a dormant bud activates and becomes an actively growing branch is complex and very finely tuned. Bud outgrowth is regulated by the interaction of environmental signals an...
journal_title:Journal of experimental botany
pub_type: 杂志文章,评审
doi:10.1093/jxb/erm134
更新日期:2008-01-01 00:00:00
abstract::Manganese (Mn) is an essential micronutrient for plant growth but is often toxic in acid or waterlogged soils. Using cowpea (Vigna unguiculata L. Walp.) grown with 0.05-1500 μM Mn in solution, two short-term (48 h) solution culture experiments examined if the effects of cations (Ca, Mg, Na, Al, or H) on Mn nutrition a...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/err097
更新日期:2011-07-01 00:00:00
abstract::The secretory cavity is a typical structure in Citrus fruit and is formed by schizolysigeny. Previous reports have indicated that programmed cell death (PCD) is involved in the degradation of secretory cavity cells in the fruit, and that the spatio-temporal location of calcium is closely related to nuclear DNA degrada...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/eraa199
更新日期:2020-08-06 00:00:00
abstract::Carotenoids are important isoprenoids produced in the plastids of photosynthetic organisms that play key roles in photoprotection and antioxidative processes. β-carotene is generated from lycopene by the lycopene β-cyclase (LCYB). Previously, we demonstrated that the introduction of the Daucus carota (carrot) DcLCYB1 ...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erab029
更新日期:2021-01-23 00:00:00
abstract::Although protein phosphorylation is integral to the regulation of protein function in diverse biological responses, relatively little is currently known about the rules that govern phosphorylation in plants. This review will discuss how the data acquired by evolving phosphoproteomic methods are beginning to fill the g...
journal_title:Journal of experimental botany
pub_type: 杂志文章,评审
doi:10.1093/jxb/erj126
更新日期:2006-01-01 00:00:00
abstract::Brassica rapa includes several important leafy vegetable crops with the potential for high cadmium (Cd) accumulation, posing a risk to human health. This study aims to understand the genetic basis underlying the variation in Cd accumulation among B. rapa vegetables. Cd uptake and translocation in 64 B. rapa accessions...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erz310
更新日期:2019-10-24 00:00:00
abstract::Topoisomerase I (topo I) is a nuclear enzyme which plays a fundamental role in several pathways involving changes in DNA topology. The topo I-mediated reaction is accomplished by the transient covalent binding of the enzyme to DNA (topo I-DNA complex). Stabilization of the topo I-DNA complex, leading to irreversible d...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erj194
更新日期:2006-01-01 00:00:00
abstract::In order to investigate the physiological role of ethylene in the initiation and subsequent progression of softening, pear fruit were treated with propylene, an analogue of ethylene or 1-methylcyclopropene (1-MCP), a gaseous inhibitor of ethylene action at the preclimacteric or ripening stages. The propylene treatment...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erg073
更新日期:2003-02-01 00:00:00