Abstract:
:A cDNA that encodes a transporter from the NHAD family was identified in Physcomitrella patens. Computer-based searches using the amino acid sequence of PpNHAD1 revealed that, in addition to being expressed in flowering plants, highly conserved transporters of this family are expressed in red algae, green algae, mosses, liverworts, and photosynthetic stramenopiles, but not in heterotrophic stramenopiles. A chloroplast transit peptide was detected in PpNHAD1 and in most of the related sequences, indicating that PpNHAD1 is a chloroplast transporter. A PpNHAD1-GFP fusion localized to the chloroplast in Physcomitrella protoplasts, and truncation of the N-terminus of the protein dispersed the fluorescence signal outside the chloroplast. PpNHAD1 did not show functional expression in either yeast or bacterial mutants, but truncated proteins with shorter N-termini, PpNHAD1-1 and PpNHAD1-2, could be functionally expressed in bacteria. PpNHAD1-1 alleviated the Li(+) intolerance of a Na(+)-efflux Escherichia coli mutant at acidic pH values. Both PpNHAD1-1 and PpNHAD1-2 reduced the K(+) requirements of a K(+)-influx E. coli mutant more actively at high pH values. PpNHAD1 seems to be an important transporter that mediates ionic homeostasis in chloroplasts from red algae to flowering plants.
journal_name
J Exp Botjournal_title
Journal of experimental botanyauthors
Barrero-Gil J,Rodríguez-Navarro A,Benito Bdoi
10.1093/jxb/erm094subject
Has Abstractpub_date
2007-01-01 00:00:00pages
2839-49issue
11eissn
0022-0957issn
1460-2431pii
erm094journal_volume
58pub_type
杂志文章abstract::A cDNA sequence coding for a pea (Pisum sativum L.) 2-Cys peroxiredoxin (2-Cys Prx) has been cloned. The deduced amino acid sequence showed a high sequence homology to the 2-Cys Prx enzymes of Phaseolus vulgaris (86%), Arabidopsis thaliana (75%), and Spinacia oleracea (75%), and contained a chloroplast target sequence...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erh238
更新日期:2004-10-01 00:00:00
abstract::Previous studies documented that metal hyperaccumulation armours plants with direct defences against pathogens. In the present study, it was found that high leaf Mn concentrations (<2500 µg g(-1)) induced grapevine resistance to powdery mildew [Uncinula necator (Schw.) Burr]. Manganese delayed pathogen spreading after...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/ers175
更新日期:2012-09-01 00:00:00
abstract::Genes encoding pathogenesis-related (PR-) proteins isolated from a cDNA library of Fusarium graminearum-infected wheat spikes of scab-resistant cultivar 'Sumai-3' were transformed into susceptible spring wheat, 'Bobwhite' using a biolistic transformation protocol, with the goal of enhancing levels of resistance agains...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erg110
更新日期:2003-03-01 00:00:00
abstract::S-RNase based gametophytic self-incompatibility (SI) is a widespread prezygotic reproductive barrier in flowering plants. In the Solanaceae, Plantaginaceae and Rosaceae gametophytic SI is controlled by the pistil-specific S-RNases and the pollen S-locus F-box proteins but non-S-specific factors, namely modifiers, are ...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erx336
更新日期:2017-11-02 00:00:00
abstract::Photosynthesis has become a major trait of interest for cereal yield improvement as breeders appear to have reached the theoretical genetic limit for harvest index, the mass of grain as a proportion of crop biomass. Yield improvements afforded by the adoption of green revolution dwarfing genes to wheat and rice are be...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/eraa077
更新日期:2020-04-06 00:00:00
abstract::ω-3 fatty acid desaturases (FADs) are thought to contribute to plant stress tolerance mainly through linolenic acid (C18:3)-induced membrane stabilization, but a comprehensive analysis of their roles in stress adaptation is lacking. Here, we isolated a microsomal ω-3 FAD gene (CbFAD3) from a cryophyte (Chorispora bung...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/ery050
更新日期:2018-04-09 00:00:00
abstract::Somatic embryogenesis in Arabidopsis is achieved by culturing bending-cotyledon embryos on a 2,4-D-containing induction medium for 14 d followed by a transfer on to a hormone-free development medium. Several genes orthologous to Arabidopsis SHOOTMERISTEMLESS (STM), CLAVATA 1 (CLV1), and ZWILLE (ZLL) were isolated from...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erq222
更新日期:2010-09-01 00:00:00
abstract::Apoplastic transport barriers in the roots of rice (Oryza sativa L. cv. IR64) and corn (Zea mays L. cv. Helix) were isolated enzymatically. Following chemical degradation (monomerization, derivatization), the amounts of aliphatic and aromatic suberin monomers were analysed quantitatively by gas chromatography and mass...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/eri144
更新日期:2005-05-01 00:00:00
abstract::HYL1 is an important regulator of microRNA (miRNA) biogenesis. A loss-of-function mutation of HYL1 causes the reduced accumulation of some miRNAs but fails to display the miRNA-deficient phenotypes of these miRNAs. In Arabidopsis, miR156 mediates phase transition through repression of SQUAMOSA PROMOTER-BINDING PROTEIN...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/err465
更新日期:2012-04-01 00:00:00
abstract::Detyrosination of α-tubulin seems to be conserved in all eukaryotes. However, its biological function in plants has remained obscure. A conserved C-terminal tyrosine is removed by a still unidentified tubulin-tyrosine carboxypeptidase (TTC) and can be religated by a tubulin-tyrosine ligase (TTL). To obtain insight int...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erv012
更新日期:2015-04-01 00:00:00
abstract::This paper outlines a numerical scheme for accurate, detailed, and high-throughput image analysis of plant roots. In contrast to existing root image analysis tools that focus on root system-average traits, a novel, fully automated and robust approach for the detailed characterization of root traits, based on a graph o...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erv359
更新日期:2015-11-01 00:00:00
abstract::Protein phosphorylation is key to the regulation of many proteins. Altered protein activity often requires the interaction of the phosphorylated protein with a class of "adapters" known as 14-3-3 proteins. This review will cover aspects of 14-3-3 interaction with key proteins of carbon and nitrogen metabolism such as ...
journal_title:Journal of experimental botany
pub_type: 杂志文章,评审
doi:10.1093/jxb/erg057
更新日期:2003-01-01 00:00:00
abstract::The garden pea has been a model for the genetics of flowering for several decades and numerous flowering loci have been identified, but until recently little was known about the molecular nature of these loci. This paper presents an update on recent work on the molecular genetics of flowering in pea, outlining progres...
journal_title:Journal of experimental botany
pub_type: 杂志文章,评审
doi:10.1093/jxb/erp120
更新日期:2009-01-01 00:00:00
abstract::In maize, water stress at flowering causes loss of kernel set and productivity. While changes in the levels of sugars and abscisic acid (ABA) are thought to play a role in this stress response, the mechanistic basis and genes involved are not known. A candidate gene approach was used with association mapping to identi...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erq308
更新日期:2011-01-01 00:00:00
abstract::Sugar beet is spring-sown for sugar production in most sugar beet-growing countries. It is grown as a vegetative crop and it accumulates yield (sugar) from very early in its growth cycle. As long as the sugar beet plants do not flower, the sugar accumulation period is indefinite and yield continues to increase. This p...
journal_title:Journal of experimental botany
pub_type: 杂志文章,评审
doi:10.1093/jxb/erp110
更新日期:2009-01-01 00:00:00
abstract::Metabolic perturbations by a gain-of-function approach provide a means to alter steady states of metabolites and query network properties, while keeping enzyme complexes intact. A combination of genetic and targeted metabolomics approach was used to understand the network properties of phenylpropanoid secondary metabo...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/ert310
更新日期:2013-11-01 00:00:00
abstract::Suberin and wax deposited in the cork (phellem) layer of the periderm form the lipophilic barrier that protects mature plant organs. Periderm lipids have been widely studied for their protective function with regards to dehydration and for how they respond to environmental stresses and wounding. However, despite advan...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erw305
更新日期:2016-10-01 00:00:00
abstract::Groundnuts (Arachis hypogaea L.) are an important crop of the semi-arid tropics where they are often exposed to maximum temperatures of > 40 degrees C for short periods during the growing season. The objectives of this study were to determine: (i) the effects of short periods of exposure to high temperature on flower ...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jexbot/51.345.777
更新日期:2000-04-01 00:00:00
abstract::Secondary salinization and its relationship to irrigation are strong incentives to improve the tolerance of crops to salinity and to drought. Achieving this through the pyramiding of physiological traits (phenotypic selection without knowledge of genotype) is feasible. However, wide application of this approach is lim...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:
更新日期:2000-01-01 00:00:00
abstract::The capacity of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) to consume RuBP is a major limitation on the rate of net CO(2) assimilation (A) in C(3) and C(4) plants. The pattern of Rubisco limitation differs between the two photosynthetic types, as shown by comparisons of temperature and CO(2) responses o...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jexbot/53.369.609
更新日期:2002-04-01 00:00:00
abstract::Flower and inflorescence reversion involve a switch from floral development back to vegetative development, thus rendering flowering a phase in an ongoing growth pattern rather than a terminal act of the meristem. Although it can be considered an unusual event, reversion raises questions about the nature and function ...
journal_title:Journal of experimental botany
pub_type: 杂志文章,评审
doi:10.1093/jxb/eri254
更新日期:2005-10-01 00:00:00
abstract::Plants of Lolium perenne were grown in sterile solution culture. 15N-labelled glycine (Gly) coupled with gas chromatograph mass spectrometry was used to prove that non-mycorrhizal plants of L. perenne are capable of acquiring N in the form of intact Gly. It was estimated that a minimum of 80% of Gly-N uptake, over a 3...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:
更新日期:2001-06-01 00:00:00
abstract::Quantitative RT-PCR (reverse transcription polymerase chain reaction, also known as qRT-PCR or real-time RT-PCR) has been used in large proportions of transcriptome analyses published to date. The accuracy of the results obtained by this method strongly depends on accurate transcript normalization using stably express...
journal_title:Journal of experimental botany
pub_type: 杂志文章,评审
doi:10.1093/jxb/ern305
更新日期:2009-01-01 00:00:00
abstract::SNARE proteins are central elements of the machinery involved in membrane fusion of eukaryotic cells. In animals and plants, SNAREs have diversified to sustain a variety of specific functions. In animals, R-SNARE proteins called brevins have diversified; in contrast, in plants, the R-SNARE proteins named longins have ...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erw094
更新日期:2016-04-01 00:00:00
abstract::The aleurone layer of cereal grains is important biologically as well as nutritionally and economically. Here, current knowledge on the regulation of aleurone development is reviewed. Recent reports suggest that the control of aleurone development is more complex than earlier models portrayed. Multiple levels of genet...
journal_title:Journal of experimental botany
pub_type: 杂志文章,评审
doi:10.1093/jxb/erq372
更新日期:2011-03-01 00:00:00
abstract::Two groups of linear gene constructs (gus and bar, and 1Ax1 and bar) lacking vector backbone sequences were independently transferred into the elite wheat (Triticum aestivum L.) variety EM12, and genetically stable transgenic plants with low copy number transgene integration were recovered. Co-transformation experimen...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erl145
更新日期:2006-01-01 00:00:00
abstract::The major source of nitrogen for rice (Oryza sativa L.) is ammonium (NH4(+)). The NH4(+) uptake of roots is mainly governed by membrane transporters, with OsAMT1;1 being a prominent member of the OsAMT1 gene family that is known to be involved in NH4(+) transport in rice plants. However, little is known about its invo...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/ert458
更新日期:2014-03-01 00:00:00
abstract::Metabolic and physiological analyses of Arabidopsis thaliana glycolate oxidase (GOX) mutant leaves were performed to understand the development of the photorespiratory phenotype after transfer from high CO2 to air. We show that two Arabidopsis genes, GOX1 and GOX2, share a redundant photorespiratory role. Air-grown si...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erw054
更新日期:2016-05-01 00:00:00
abstract::When plants encounter compacted soil, stomatal closure occurs and shoot growth slows. These responses occur in the absence of detectable changes in foliar water status. The use of genotypes with a reduced capacity to synthesize either ABA or ethylene has provided convincing evidence that ABA is responsible for providi...
journal_title:Journal of experimental botany
pub_type: 杂志文章,评审
doi:
更新日期:2002-01-01 00:00:00
abstract::An investigation aimed at a better understanding of the molecular adaptation mechanisms of salt stress was carried out in 7-d-old tomato Solanum lycopersicum (L.) Mill cultivars Patio and 'F144', using a proteomic approach. Total proteins were extracted from radicles and hypocotyls collected from both non-saline contr...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erp075
更新日期:2009-01-01 00:00:00