Abstract:
:This paper outlines a numerical scheme for accurate, detailed, and high-throughput image analysis of plant roots. In contrast to existing root image analysis tools that focus on root system-average traits, a novel, fully automated and robust approach for the detailed characterization of root traits, based on a graph optimization process is presented. The scheme, firstly, distinguishes primary roots from lateral roots and, secondly, quantifies a broad spectrum of root traits for each identified primary and lateral root. Thirdly, it associates lateral roots and their properties with the specific primary root from which the laterals emerge. The performance of this approach was evaluated through comparisons with other automated and semi-automated software solutions as well as against results based on manual measurements. The comparisons and subsequent application of the algorithm to an array of experimental data demonstrate that this method outperforms existing methods in terms of accuracy, robustness, and the ability to process root images under high-throughput conditions.
journal_name
J Exp Botjournal_title
Journal of experimental botanyauthors
Cai J,Zeng Z,Connor JN,Huang CY,Melino V,Kumar P,Miklavcic SJdoi
10.1093/jxb/erv359subject
Has Abstractpub_date
2015-11-01 00:00:00pages
6551-62issue
21eissn
0022-0957issn
1460-2431pii
erv359journal_volume
66pub_type
杂志文章abstract::We aim to incorporate deep root traits into future wheat varieties to increase access to stored soil water during grain development, which is twice as valuable for yield as water captured at younger stages. Most root phenotyping efforts have been indirect studies in the laboratory, at young plant stages, or using indi...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/eru250
更新日期:2014-11-01 00:00:00
abstract::AGAMOUS (AG) MADS-box transcription factors have been shown to play crucial roles in floral organ and fruit development in angiosperms. Here, we isolated a tomato (Solanum lycopersicum) AG MADS-box gene SlMBP3 and found that it is preferentially expressed in flowers and during early fruit developmental stages in the w...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/ery418
更新日期:2019-02-05 00:00:00
abstract::Several root anatomical phenes affect water acquisition from drying soil, and may therefore have utility in breeding more drought-tolerant crops. Anatomical phenes that reduce the metabolic cost of the root cortex ('cortical burden') improve soil exploration and therefore water acquisition from drying soil. The best e...
journal_title:Journal of experimental botany
pub_type: 杂志文章,评审
doi:10.1093/jxb/eru162
更新日期:2014-11-01 00:00:00
abstract::Root growth is modulated by different factors, including phytohormones, transcription factors, and microRNAs (miRNAs). MicroRNA156 and its targets, the SQUAMOSA PROMOTER BINDING PROTEIN-LIKE (SPL) genes, define an age-dependent pathway that controls several developmental processes, including lateral root emergence. Ho...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erz475
更新日期:2020-01-23 00:00:00
abstract::Cytophotometry of individual nuclei was used to examine the level of endoreduplication in epidermal cells from the upper and lower parts of the ovary during Ornithogalum umbellatum flower and fruit development. An increase in DNA content from 2-4C to 2-8C in both parts of the ovary was observed, while the epidermal ce...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erm071
更新日期:2007-01-01 00:00:00
abstract::Members of the Orchidaceae, one of the largest families of flowering plants, evolved the crassulacean acid metabolism (CAM) photosynthesis strategy. It is thought that CAM triggers adaptive radiation into new niche spaces, yet very little is known about its origin and diversification on different continents. Here, we ...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erz461
更新日期:2019-11-29 00:00:00
abstract::Since cysteine is the first committed molecule in plant metabolism containing both sulphur and nitrogen, the regulation of its biosynthesis is critically important. Cysteine itself is required for the production of an abundance of key metabolites in diverse pathways. Plants alter their metabolism to compensate for sul...
journal_title:Journal of experimental botany
pub_type: 杂志文章,评审
doi:10.1093/jxb/erh136
更新日期:2004-06-01 00:00:00
abstract::The promoter of the Arabidopsis thaliana L. AtEm1 gene encoding a late embryogenesis abundant protein was fused to the beta-glucuronidase reporter gene and introduced into Brassica napus. The promoter is highly active in the vascular tissues of embryo and pollen grains and also active in petals, sepals, caulinar leave...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jexbot/52.360.1587
更新日期:2001-07-01 00:00:00
abstract::The floral repressor APETALA2 (AP2) in Arabidopsis regulates flowering through the age pathway. The AP2 ortholog in the alpine perennial Arabis alpina, PERPETUAL FLOWERING 2 (PEP2), was previously reported to control flowering through the vernalization pathway via enhancing the expression of another floral repressor P...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/ery423
更新日期:2019-02-05 00:00:00
abstract::A model of kiwifruit berry development is presented, building on the model of Fishman and Génard used for peach fruit. That model has been extended to incorporate a number of important features of kiwifruit growth. First, the kiwifruit berry is attached to the stem through a pedicel/receptacle complex which contribute...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/ert317
更新日期:2013-12-01 00:00:00
abstract::As a first step to investigate whether Rab GTPases are involved in grape berry development, the Vitis vinifera EST and gene databases were searched for members of the VvRab family. The grapevine genome was found to contain 26 VvRabs that could be distributed into all of the eight groups described in the literature for...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/ern132
更新日期:2008-01-01 00:00:00
abstract::Pelargonium graveolens is a wild predecessor to rose-scented geranium hybrids prized for their essential oils used as fragrances and flavorings. However, little is known about their biosynthesis. Here we present metabolic evidence that at least two distinct monoterpene biosynthetic pathways contribute to their volatil...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erz397
更新日期:2020-01-01 00:00:00
abstract::During grain filling in barley (Hordeum vulgare L. cv. Barke) reserves are remobilized from vegetative organs. Glumes represent the vegetative tissues closest to grains, senesce late, and are involved in the conversion of assimilates. To analyse glume development and metabolism related to grain filling, parallel trans...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/eru492
更新日期:2015-03-01 00:00:00
abstract::The absence of a terminal flower in inflorescences ('open inflorescences') is currently explained by the maintenance of putative stem-cells in the central zone (CZ) of the inflorescence meristem (IM) governed by the CLAVATA-WUSCHEL regulatory loop. Disruption of this regulatory pathway, as in Arabidopsis TERMINAL FLOW...
journal_title:Journal of experimental botany
pub_type: 杂志文章,评审
doi:10.1093/jxb/erq262
更新日期:2011-01-01 00:00:00
abstract::The role of nitrate reduction to produce nitric oxide (NO) and its subsequent oxidation by oxyhaemoglobin as a mechanism to maintain plant cell energetics during hypoxia is examined. Nitrate reduction in hypoxic conditions can be considered as an alternative respiratory pathway, with nitrate as an intermediate electro...
journal_title:Journal of experimental botany
pub_type: 杂志文章,评审
doi:10.1093/jxb/erh272
更新日期:2004-12-01 00:00:00
abstract::It is generally believed that an osmotically generated pressure gradient drives the phloem mass flow. So far, this widely accepted Münch theory has required remarkably few adaptations, but the debate on alternative and additional hypotheses is still ongoing. Recently, a possible shortcoming of the Münch theory has bee...
journal_title:Journal of experimental botany
pub_type: 杂志文章,评审
doi:10.1093/jxb/ert302
更新日期:2013-11-01 00:00:00
abstract::Leaves and callus of Kalanchoë daigremontiana and Taxus brevifolia were used to investigate nitric oxide-induced apoptosis in plant cells. The effect of nitric oxide (NO) was studied by using a NO donor, sodium nitroprusside (SNP), a nitric oxide-synthase (NOS) inhibitor, N:(G)-monomethyl-L-arginine (NMMA), and centri...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jexbot/51.347.1027
更新日期:2000-06-01 00:00:00
abstract::Despite the economic importance of grasses as food, feed, and energy crops, little is known about the genes that control their cell wall synthesis, assembly, and remodelling. Here a detailed transcriptome analysis that allowed the identification of genes involved in grass cell wall biogenesis is provided. Differential...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/err045
更新日期:2011-06-01 00:00:00
abstract::Classical and recently found phytohormones play an important role in plant growth and development, but plants additionally control these processes through small signalling peptides. Over 1000 potential small signalling peptide sequences are present in the Arabidopsis genome. However, to date, a mere handful of small s...
journal_title:Journal of experimental botany
pub_type: 杂志文章,评审
doi:10.1093/jxb/ert283
更新日期:2013-12-01 00:00:00
abstract::Plants of Lolium perenne L. were grown in sterile solution culture supplied with 2 mol m(-3) nitrogen as either nitrate or ammonium. Glutamine at 5 mol m(-3) was added to the nutrient solution of half the plants for 24 h. Root nitrate influx (at external nitrate concentrations 0-2000 mmol m(-3)) and amino acid concent...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erh066
更新日期:2004-03-01 00:00:00
abstract::Most rhizobial strains inhibit rice root growth in the presence of calcium or potassium nitrates, but not ammonium nitrate. Certain rhizobial strains, however, such as strain R4, do not inhibit rice growth and can enter rice roots and multiply in the intercellular spaces. By using the green fluorescent protein (GFP) a...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erm181
更新日期:2007-01-01 00:00:00
abstract::Potted grapevines of 140 Ruggeri (Vitis berlandieri × Vitis rupestris), a good Cl(-) excluder, and K 51-40 (Vitis champinii × Vitis riparia 'Gloire'), a poor Cl(-) excluder, and of a family obtained by crossing the two genotypes, were used to examine the inheritance of Cl(-) exclusion. Rooted leaves were then used to ...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erq326
更新日期:2011-01-01 00:00:00
abstract::The chance to watch floral organs develop live is not to be missed! Here, we outline reasons why quantitative, live-cell imaging is an important approach to study floral morphogenesis, and provide a basic workflow of how to get started. We highlight key advances in morphodynamics of lateral organ development, and disc...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/eraa038
更新日期:2020-05-09 00:00:00
abstract::Pectins are major components of plant primary cell walls. They include homogalacturonans (HGs), which are the most abundant pectin and can be the target of apoplastic enzymes like pectin methylesterases (PMEs) that control their methylesterification level. Several PMEs are expressed in the seed coat of Arabidopsis tha...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erw025
更新日期:2016-04-01 00:00:00
abstract::Heterotrimeric G-proteins (G-proteins) have been implicated in ubiquitous signalling mechanisms in eukaryotes. In plants, G-proteins modulate hormonal and stress responses and regulate diverse developmental processes. However, the molecular mechanisms of their functions are largely unknown. A yeast two-hybrid screen w...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/ert327
更新日期:2013-12-01 00:00:00
abstract::The plant-specific NAC transcription factors have attracted particular attention because of their involvement in stress responses, senescence, and nutrient remobilization. The HvNAC005 gene of barley encodes a protein belonging to subgroup NAC-a6 of the NAC family. This study shows that HvNAC005 is associated with dev...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erw286
更新日期:2016-09-01 00:00:00
abstract::Nutrient requirements for plant growth are expected to rise in response to the predicted changes in CO(2) and temperature. In this context, little attention has been paid to the effects of soil temperature, which limits plant growth at early stages in temperate regions. A factorial growth-room experiment was conducted...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jexbot/52.362.1913
更新日期:2001-09-01 00:00:00
abstract::The timing of seed germination is crucial for seed plants and is coordinated by internal and external cues, reflecting adaptations to different habitats. Physiological and molecular studies with lettuce and Arabidopsis thaliana have documented a strict requirement for light to initiate germination and identified many ...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erz146
更新日期:2019-06-28 00:00:00
abstract::Plant mitochondrial genes contain cis- and trans-group II introns that must be spliced before translation. The mechanism by which these introns are spliced is not well understood. Several families of proteins have been implicated in the intron splicing, of which the pentatricopeptide repeat (PPR) proteins are proposed...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/ery432
更新日期:2019-02-05 00:00:00
abstract::The phytohormone abscisic acid (ABA) plays pivotal roles in the regulation of developmental and environmental responses in plants. Identification of cytoplasmic ABA receptors enabled the elucidation of the main ABA signalling pathway, connecting ABA perception to either nuclear events or the action of several transpor...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/ers188
更新日期:2012-09-01 00:00:00