Abstract：
:Plants of Lolium perenne were grown in sterile solution culture. 15N-labelled glycine (Gly) coupled with gas chromatograph mass spectrometry was used to prove that non-mycorrhizal plants of L. perenne are capable of acquiring N in the form of intact Gly. It was estimated that a minimum of 80% of Gly-N uptake, over a 3 h period, was as intact Gly, though possible processes resulting in deviation from this estimate are discussed. The relative incorporation of 15N derived from Gly uptake into serine (Ser) compared with other amino acids in the root amino acid pool suggested the enzyme serine:glyoxylate aminotransferase was at least partly responsible for the synthesis of Ser from Gly. Defoliation was shown to reduce Gly uptake by L. perenne. The addition of either 25 mol x m(-3) sucrose or 50 mol x m(-3) glucose to the uptake solution of defoliated plants increased Gly-N uptake compared with both defoliated plants without sugars and with undefoliated plants. Addition of a glucose analogue, 3-O-methyl-D-glucopyranose, that is absorbed but not metabolized by plants, did not affect Gly uptake by defoliated plants. Increasing pH from 3.5 to 9.2 caused a reduction in Gly uptake. Results of the effects of defoliation and pH are consistent with Gly uptake by L. perenne being by an energy-dependent proton symport. When either or Gly were supplied to plants at equimolar concentrations, uptake was five times greater than that of Gly at pH 6 and 13 times greater at pH 9.
journal_name
J Exp Botjournal_title
Journal of experimental botanyauthors
Thornton Bkeywords:
subject
Has Abstractpub_date
2001-06-01 00:00:00pages
1315-22issue
359eissn
0022-0957issn
1460-2431journal_volume
52pub_type
杂志文章abstract：:The transgenic traits associated with the majority of commercial genetically modified crops are focused on improving herbicide and insecticide management practices. The use of the transgenic technology in these crops and the associated chemistry has been the basis of studies that provide evidence for occasional improv...
journal_title：Journal of experimental botany
pub_type： 杂志文章
doi：10.1093/jxb/err332
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journal_title：Journal of experimental botany
pub_type： 杂志文章
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journal_title：Journal of experimental botany
pub_type： 杂志文章
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journal_title：Journal of experimental botany
pub_type： 杂志文章,评审
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更新日期：2018-01-23 00:00:00
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journal_title：Journal of experimental botany
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journal_title：Journal of experimental botany
pub_type： 杂志文章,评审
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journal_title：Journal of experimental botany
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journal_title：Journal of experimental botany
pub_type： 杂志文章
doi：10.1093/jxb/eraa586
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journal_title：Journal of experimental botany
pub_type： 杂志文章
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journal_title：Journal of experimental botany
pub_type： 杂志文章
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更新日期：2009-01-01 00:00:00
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journal_title：Journal of experimental botany
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journal_title：Journal of experimental botany
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journal_title：Journal of experimental botany
pub_type： 杂志文章
doi：10.1093/jxb/erh111
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journal_title：Journal of experimental botany
pub_type： 杂志文章
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journal_title：Journal of experimental botany
pub_type： 杂志文章
doi：10.1093/jxb/48.Special_Issue.539
更新日期：1997-03-01 00:00:00
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journal_title：Journal of experimental botany
pub_type： 杂志文章
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更新日期：2002-09-01 00:00:00
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journal_title：Journal of experimental botany
pub_type： 杂志文章
doi：10.1093/jxb/ery029
更新日期：2018-04-09 00:00:00
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journal_title：Journal of experimental botany
pub_type： 杂志文章
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更新日期：2015-06-01 00:00:00
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journal_title：Journal of experimental botany
pub_type： 杂志文章
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journal_title：Journal of experimental botany
pub_type： 杂志文章
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journal_title：Journal of experimental botany
pub_type： 杂志文章,评审
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更新日期：2016-01-01 00:00:00
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journal_title：Journal of experimental botany
pub_type： 杂志文章
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更新日期：2015-10-01 00:00:00
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journal_title：Journal of experimental botany
pub_type： 杂志文章
doi：10.1093/jxb/erp286
更新日期：2010-01-01 00:00:00
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journal_title：Journal of experimental botany
pub_type： 杂志文章,评审
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更新日期：2001-03-01 00:00:00
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journal_title：Journal of experimental botany
pub_type： 杂志文章
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journal_title：Journal of experimental botany
pub_type： 杂志文章
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journal_title：Journal of experimental botany
pub_type： 杂志文章
doi：10.1093/jxb/ers376
更新日期：2013-02-01 00:00:00
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journal_title：Journal of experimental botany
pub_type： 杂志文章,评审
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更新日期：2019-11-29 00:00:00
