Abstract:
:Genes encoding pathogenesis-related (PR-) proteins isolated from a cDNA library of Fusarium graminearum-infected wheat spikes of scab-resistant cultivar 'Sumai-3' were transformed into susceptible spring wheat, 'Bobwhite' using a biolistic transformation protocol, with the goal of enhancing levels of resistance against scab. Twenty-four putative transgenic lines expressing either a single PR-protein gene or combinations thereof were regenerated. Transgene expression in a majority of these lines (20) was completely silenced in the T(1) or T(2) generations. Four transgenic wheat lines showed stable inheritance and expression of either a single transgene or transgene combinations up to four generations. One line co-expressing a chitinase and beta-1,3-glucanase gene combination, when bioassayed against scab showed a delay in the spread of the infection (type II resistance) under greenhouse conditions. This line and a second transgenic line expressing a rice thaumatin-like protein gene (tlp) which had moderate resistance to scab in previous greenhouse trials, along with susceptible and resistance checks were evaluated for resistance to scab under field conditions. None of the transgenic lines had resistance to scab in the field under conditions of strong pathogen, suggesting these plants lacked effective resistance to initial infection (type I resistance) under these conditions. As far as is known, this is the first report of field evaluation of transgenic wheat expressing genes for PR-proteins against disease resistance.
journal_name
J Exp Botjournal_title
Journal of experimental botanyauthors
Anand A,Zhou T,Trick HN,Gill BS,Bockus WW,Muthukrishnan Sdoi
10.1093/jxb/erg110keywords:
subject
Has Abstractpub_date
2003-03-01 00:00:00pages
1101-11issue
384eissn
0022-0957issn
1460-2431journal_volume
54pub_type
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journal_title:Journal of experimental botany
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journal_title:Journal of experimental botany
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journal_title:Journal of experimental botany
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journal_title:Journal of experimental botany
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journal_title:Journal of experimental botany
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journal_title:Journal of experimental botany
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journal_title:Journal of experimental botany
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更新日期:2008-01-01 00:00:00
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journal_title:Journal of experimental botany
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journal_title:Journal of experimental botany
pub_type: 杂志文章,评审
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journal_title:Journal of experimental botany
pub_type: 杂志文章
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journal_title:Journal of experimental botany
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journal_title:Journal of experimental botany
pub_type: 杂志文章
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journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:
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