Normalization of qRT-PCR data: the necessity of adopting a systematic, experimental conditions-specific, validation of references.

abstract：:Linking our understanding of biological processes at different scales is a major conceptual challenge in biology and aggravated by differences in research methods. Modelling can be a useful approach to consolidating our understanding across traditional research domains. The laboratory model species Arabidopsis is very...

journal_title：Journal of experimental botany

authors： Zardilis A,Hume A,Millar AJ

更新日期：2019-04-29 00:00:00

abstract：:One of the most important roles of plant roots is to take up essential mineral nutrients from the soil for use in plant growth and development. The uptake of mineral elements is mediated by various transporters belonging to different transporter families. Here we reviewed transporters for the uptake of macronutrients ...

journal_title：Journal of experimental botany

authors： Sasaki A,Yamaji N,Ma JF

更新日期：2016-06-01 00:00:00

abstract：:The importance of gene duplication for evolutionary diversification has been mainly discussed in terms of genetic redundancy allowing neofunctionalization. In the case of C4 photosynthesis, which evolved via the co-option of multiple enzymes to boost carbon fixation in tropical conditions, the importance of genetic re...

journal_title：Journal of experimental botany

authors： Bianconi ME,Dunning LT,Moreno-Villena JJ,Osborne CP,Christin PA

更新日期：2018-04-09 00:00:00

abstract：:The transcriptomes of senescing flag leaves collected from barley field plots with standard or high nitrogen supply were compared to identify genes specifically associated with nitrogen remobilization during leaf senescence under agronomically relevant conditions. In flag leaves collected in field plots with high nitr...

journal_title：Journal of experimental botany

authors： Hollmann J,Gregersen PL,Krupinska K

更新日期：2014-07-01 00:00:00

abstract：:Successful plant reproduction relies on the perfect orchestration of singular processes that culminate in the product of reproduction: the seed. The floral transition, floral organ development, and fertilization are well-studied processes and the genetic regulation of the various steps is being increasingly unveiled. ...

journal_title：Journal of experimental botany

authors： Pajoro A,Biewers S,Dougali E,Leal Valentim F,Mendes MA,Porri A,Coupland G,Van de Peer Y,van Dijk AD,Colombo L,Davies B,Angenent GC

更新日期：2014-09-01 00:00:00

abstract：:The transition from reversible to permanent wilting, in whole tomato seedlings (Lycopersicon esculentum Mill. cv. M82) following severe salt-stress by root exposure to 300 mM NaCl, was investigated. Salinized seedlings wilted rapidly but recovered if returned to non-saline nutrient solution within 6 h. However, after ...

journal_title：Journal of experimental botany

authors： Shalata A,Neumann PM

更新日期：2001-11-01 00:00:00

abstract：:Leaves and callus of Kalanchoë daigremontiana and Taxus brevifolia were used to investigate nitric oxide-induced apoptosis in plant cells. The effect of nitric oxide (NO) was studied by using a NO donor, sodium nitroprusside (SNP), a nitric oxide-synthase (NOS) inhibitor, N:(G)-monomethyl-L-arginine (NMMA), and centri...

journal_title：Journal of experimental botany

authors： Pedroso MC,Magalhaes JR,Durzan D

更新日期：2000-06-01 00:00:00

abstract：:The effect of water stress on stomatal closure in sunflower plants has been found to be dependent on K(+) nutrient status. When plants with different internal K(+) content were subjected to a water-stress period, stomatal conductance was reduced more markedly in plants with an adequate K(+) supply than in K(+)-starved...

journal_title：Journal of experimental botany

authors： Benlloch-González M,Romera J,Cristescu S,Harren F,Fournier JM,Benlloch M

更新日期：2010-02-01 00:00:00

abstract：:This article reviews the historical development of cytology and cytogenetics in Arabidopsis, and summarizes recent developments in molecular cytogenetics, with special emphasis on meiotic studies. Despite the small genome and small chromosomes of Arabidopsis, considerable progress has been made in developing appropria...

journal_title：Journal of experimental botany

authors： Armstrong SJ,Jones GH

更新日期：2003-01-01 00:00:00

abstract：:The transcription factor Woolly (Wo) and its downstream gene CycB2 have been shown to regulate trichome development in tomato (Solanum lycopersicum). It has been demonstrated that only the gain-of-function allele of Slwo (SlWoV, the Slwo woolly motif mutant allele) can increase the trichome density; however, it remain...

journal_title：Journal of experimental botany

authors： Wu ML,Cui YC,Ge L,Cui LP,Xu ZC,Zhang HY,Wang ZJ,Zhou D,Wu S,Chen L,Cui H

更新日期：2020-03-25 00:00:00

abstract：:Soil respiration is affected by distributions of roots and soil carbon substrates and by temperature and soil water content, all of which vary spatially and temporally. The objective of this paper was to compare a manual system for measuring soil respiration in a temperate forest, which had a greater spatial distribut...

journal_title：Journal of experimental botany

authors： Savage KE,Davidson EA

更新日期：2003-03-01 00:00:00

abstract：:Pelargonium graveolens is a wild predecessor to rose-scented geranium hybrids prized for their essential oils used as fragrances and flavorings. However, little is known about their biosynthesis. Here we present metabolic evidence that at least two distinct monoterpene biosynthetic pathways contribute to their volatil...

journal_title：Journal of experimental botany

authors： Bergman ME,Chávez Á,Ferrer A,Phillips MA

更新日期：2020-01-01 00:00:00

abstract：:Six MaMADS-box genes have been cloned from the banana fruit cultivar Grand Nain. The similarity of these genes to tomato LeRIN is low and neither MaMADS2 nor MaMADS1 complement the tomato rin mutation. Nevertheless, the expression patterns, specifically in fruit and the induction during ripening and in response to eth...

journal_title：Journal of experimental botany

authors： Elitzur T,Vrebalov J,Giovannoni JJ,Goldschmidt EE,Friedman H

更新日期：2010-03-01 00:00:00

abstract：:ABC transporters are ubiquitously present in all kingdoms and mediate the transport of a large spectrum of structurally different compounds. Plants possess high numbers of ABC transporters in relation to other eukaryotes; the ABCG subfamily in particular is extensive. Earlier studies demonstrated that ABCG transporter...

journal_title：Journal of experimental botany

authors： Gräfe K,Schmitt L

更新日期：2021-01-20 00:00:00

abstract：:Heat-shock protein 90 (HSP90) is a highly conserved molecular chaperone that is involved in modulating a multitude of cellular processes under both physiological and stress conditions. In Arabidopsis, there are seven HSP90 isoforms (HSP90.1-HSP90.7) that are localized in the cytoplasm/nucleus, mitochondrion, chloropla...

journal_title：Journal of experimental botany

authors： Chong LP,Wang Y,Gad N,Anderson N,Shah B,Zhao R

更新日期：2015-01-01 00:00:00

abstract：:Individuals, families, networks, and botanic gardens have made records of flowering times of a wide range of plant species over many years. These data can highlight year to year changes in seasonal events (phenology) and those datasets covering long periods draw interest for their perspective on plant responses to cli...

journal_title：Journal of experimental botany

authors： Tooke F,Battey NH

更新日期：2010-06-01 00:00:00

abstract：:Abscisic acid conjugate concentrations increased in barley xylem sap under salinity, whereas it remained at a low level in the intercellular washing fluid (IWF) of barley primary leaves (Hordeum vulgare cv. Gerbel). Here it is shown that IWF contains beta-glucosidase activity which releases abscisic acid (ABA) from th...

journal_title：Journal of experimental botany

authors： Dietz KJ,Sauter A,Wichert K,Messdaghi D,Hartung W

更新日期：2000-05-01 00:00:00

abstract：:Protein tyrosine nitration is a post-translational modification mediated by reactive nitrogen species (RNS) that is associated with nitro-oxidative damage. No information about this process is available in relation to higher plants during development and senescence. Using pea plants at different developmental stages (...

journal_title：Journal of experimental botany

authors： Begara-Morales JC,Chaki M,Sánchez-Calvo B,Mata-Pérez C,Leterrier M,Palma JM,Barroso JB,Corpas FJ

更新日期：2013-02-01 00:00:00

abstract：:The genomes of many plant viruses have a coding capacity limited to <10 proteins, yet it is becoming increasingly clear that individual plant virus proteins may interact with several targets in the host for establishment of infection. As new functions are uncovered for individual viral proteins, virologists have reali...

journal_title：Journal of experimental botany

authors： Schoelz JE,Angel CA,Nelson RS,Leisner SM

更新日期：2016-03-01 00:00:00

abstract：:The plant root system is important for the uptake of water and nutrients and the anchoring of plants in the soil. Lateral roots (LRs) contribute considerably to root system architecture. Their post-embryonic formation is regulated by hormones and environmental cues. The hormone cytokinin influences LR formation and gr...

journal_title：Journal of experimental botany

authors： Chang L,Ramireddy E,Schmülling T

更新日期：2013-11-01 00:00:00

abstract：:Symbiotic nitrogen fixation carried out in legume root nodules requires transition metals. These nutrients are delivered by the host plant to the endosymbiotic nitrogen-fixing bacteria living within the nodule cells, a process in which vascular transport is essential. As members of the Yellow Stripe-Like (YSL) family ...

journal_title：Journal of experimental botany

authors： Castro-Rodríguez R,Abreu I,Reguera M,Novoa-Aponte L,Mijovilovich A,Escudero V,Jiménez-Pastor FJ,Abadía J,Wen J,Mysore KS,Álvarez-Fernández A,Küpper H,Imperial J,González-Guerrero M

更新日期：2020-12-31 00:00:00

abstract：:The integration of signals received by a cell, and their transduction to targets, is essential for all cellular responses. The cytoskeleton has been identified as a major target of signalling cascades in both animal and plant cells. Self-incompatibility (SI) in Papaver rhoeas involves an allele-specific recognition be...

journal_title：Journal of experimental botany

authors： Staiger CJ,Franklin-Tong VE

更新日期：2003-01-01 00:00:00

abstract：:Capsella is a small genus within the mustard family (Brassicaceae). Its three species, however, show many evolutionary trends also observed in other Brassicaceae (including Arabidopsis) and far beyond, including transitions from a diploid, self-incompatible, obligatory outcrossing species with comparatively large and ...

journal_title：Journal of experimental botany

authors： Hintz M,Bartholmes C,Nutt P,Ziermann J,Hameister S,Neuffer B,Theissen G

更新日期：2006-01-01 00:00:00

abstract：:Both strigolactones (SLs) and abscisic acid (ABA) biosynthetically originate from carotenoids. Considering their common origin, the interaction of these two hormones at the biosynthetic and/or regulatory level may be anticipated. Here we show that, in rice, drought simultaneously induces SL production in the root, and...

journal_title：Journal of experimental botany

authors： Haider I,Andreo-Jimenez B,Bruno M,Bimbo A,Floková K,Abuauf H,Ntui VO,Guo X,Charnikhova T,Al-Babili S,Bouwmeester HJ,Ruyter-Spira C

更新日期：2018-04-23 00:00:00

abstract：:14C-salicylic acid (SA) was used to monitor SA metabolism and its regulation in tobacco cell suspension culture. Two SA concentrations (20 microM and 200 microM) were used for comparison. SA was quickly taken up in both treatments, and the 200 microM-treated cells absorbed approximately 15 times that of 20 microM-trea...

journal_title：Journal of experimental botany

authors： Chen HJ,Hou WC,Kuć J,Lin YH

更新日期：2001-06-01 00:00:00

abstract：:The C4 photosynthetic pathway uses water more efficiently than the C3 type, yet biogeographical analyses show a decline in C4 species relative to C3 species with decreasing rainfall. To investigate this paradox, the hypothesis that the C4 advantage over C3 photosynthesis is diminished by drought was tested, and the un...

journal_title：Journal of experimental botany

authors： Ripley BS,Gilbert ME,Ibrahim DG,Osborne CP

更新日期：2007-01-01 00:00:00

abstract：:The chance to watch floral organs develop live is not to be missed! Here, we outline reasons why quantitative, live-cell imaging is an important approach to study floral morphogenesis, and provide a basic workflow of how to get started. We highlight key advances in morphodynamics of lateral organ development, and disc...

journal_title：Journal of experimental botany

authors： Rambaud-Lavigne L,Hay A

更新日期：2020-05-09 00:00:00

abstract：:The cotyledon represents one of the bases of classification within the plant kingdom, providing the name-giving difference between dicotyledonous and monocotyledonous plants. It is also a fundamental organ and there have been many reports of cotyledon mutants in many species. The use of these mutants where they have a...

journal_title：Journal of experimental botany

authors： Chandler JW

更新日期：2008-01-01 00:00:00

abstract：:Leghemoglobins transport and deliver O2 to the symbiosomes inside legume nodules and are essential for nitrogen fixation. However, the roles of other hemoglobins (Hbs) in the rhizobia-legume symbiosis are unclear. Several Lotus japonicus mutants affecting LjGlb1-1, a non-symbiotic class 1 Hb, have been used to study t...

journal_title：Journal of experimental botany

authors： Fukudome M,Calvo-Begueria L,Kado T,Osuki K,Rubio MC,Murakami E,Nagata M,Kucho K,Sandal N,Stougaard J,Becana M,Uchiumi T

更新日期：2016-09-01 00:00:00

abstract：:Rapid alkalinization factor (RALF) is a peptide signal that plays a basic role in cell biology and most likely regulates cell expansion. In this study, transgenic Arabidopsis thaliana lines with high and low levels of AtRALF1 transcripts were used to investigate this peptide's mechanism of action. Overexpression of th...

journal_title：Journal of experimental botany

authors： Bergonci T,Ribeiro B,Ceciliato PH,Guerrero-Abad JC,Silva-Filho MC,Moura DS

更新日期：2014-05-01 00:00:00