The Medicago truncatula Yellow Stripe1-Like3 gene is involved in vascular delivery of transition metals to root nodules.

Abstract:

:Symbiotic nitrogen fixation carried out in legume root nodules requires transition metals. These nutrients are delivered by the host plant to the endosymbiotic nitrogen-fixing bacteria living within the nodule cells, a process in which vascular transport is essential. As members of the Yellow Stripe-Like (YSL) family of metal transporters are involved in root to shoot transport, they should also be required for root to nodule metal delivery. The genome of the model legume Medicago truncatula encodes eight YSL proteins, four of them with a high degree of similarity to Arabidopsis thaliana YSLs involved in long-distance metal trafficking. Among them, MtYSL3 is a plasma membrane protein expressed by vascular cells in roots and nodules and by cortical nodule cells. Reducing the expression level of this gene had no major effect on plant physiology when assimilable nitrogen was provided in the nutrient solution. However, nodule functioning was severely impaired, with a significant reduction of nitrogen fixation capabilities. Further, iron and zinc accumulation and distribution changed. Iron was retained in the apical region of the nodule, while zinc became strongly accumulated in the nodule veins in the ysl3 mutant. These data suggest a role for MtYSL3 in vascular delivery of iron and zinc to symbiotic nitrogen fixation.

journal_name

J Exp Bot

authors

Castro-Rodríguez R,Abreu I,Reguera M,Novoa-Aponte L,Mijovilovich A,Escudero V,Jiménez-Pastor FJ,Abadía J,Wen J,Mysore KS,Álvarez-Fernández A,Küpper H,Imperial J,González-Guerrero M

doi

10.1093/jxb/eraa390

subject

Has Abstract

pub_date

2020-12-31 00:00:00

pages

7257-7269

issue

22

eissn

0022-0957

issn

1460-2431

pii

5897034

journal_volume

71

pub_type

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