Suppression of tubulin detyrosination by parthenolide recruits the plant-specific kinesin KCH to cortical microtubules.

Abstract:

:Detyrosination of α-tubulin seems to be conserved in all eukaryotes. However, its biological function in plants has remained obscure. A conserved C-terminal tyrosine is removed by a still unidentified tubulin-tyrosine carboxypeptidase (TTC) and can be religated by a tubulin-tyrosine ligase (TTL). To obtain insight into the still elusive biological function of this detyrosination-tyrosination cycle, the effects of the TTC inhibitor parthenolide were analysed in BY-2 tobacco cells. Parthenolide caused a depletion of detyrosinated α-tubulin, whereas the level of tyrosinated tubulin was elevated. This biochemical effect was accompanied by growth inhibition in cycling BY-2 cells and alteration of microtubule-dependent events that define division and expansion geometry such as cell plate alignment or axial expansion. Furthermore, parthenolide triggered an apoplastic alkalinization indicative of activation of defence-related calcium influx channels. At the same time, parthenolide promoted the association of the plant-specific kinesin KCH with cortical microtubules. These observations are integrated into a working model, where detyrosination acts as signal to modulate the binding of kinesin motors involved in structural and sensory functions of the microtubular cytoskeleton.

journal_name

J Exp Bot

authors

Schneider N,Ludwig H,Nick P

doi

10.1093/jxb/erv012

subject

Has Abstract

pub_date

2015-04-01 00:00:00

pages

2001-11

issue

7

eissn

0022-0957

issn

1460-2431

pii

erv012

journal_volume

66

pub_type

杂志文章
  • Analysis of carbohydrate metabolism enzymes and cellular contents of sugars and proteins during spruce somatic embryogenesis suggests a regulatory role of exogenous sucrose in embryo development.

    abstract::Carbohydrate metabolism was investigated during spruce somatic embryogenesis. During the period of maintenance corresponding to the active phase of embryogenic tissue growth, activities of soluble acid invertase and alkaline invertase increased together with cellular glucose and fructose levels. During the same time, ...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jexbot/52.365.2301

    authors: Iraqi D,Tremblay FM

    更新日期:2001-12-01 00:00:00

  • To what extent may changes in the root system architecture of Arabidopsis thaliana grown under contrasted homogenous nitrogen regimes be explained by changes in carbon supply? A modelling approach.

    abstract::Root system architecture adapts to low nitrogen (N) nutrition. Some adaptations may be mediated by modifications of carbon (C) fluxes. The objective of this study was to test the hypothesis that changes in root system architecture under different N regimes may be accounted for by using simple hypotheses of C allocatio...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章,评审

    doi:10.1093/jxb/erq090

    authors: Brun F,Richard-Molard C,Pagès L,Chelle M,Ney B

    更新日期:2010-05-01 00:00:00

  • High-level overexpression of the Arabidopsis HsfA2 gene confers not only increased themotolerance but also salt/osmotic stress tolerance and enhanced callus growth.

    abstract::Heat shock transcription factors (Hsfs) are the central regulators of the heat shock (HS) stress response in all eukaryotic organisms. HsfA2 is one of the Arabidopsis class A Hsfs, and the induction of HsfA2 expression in response to HS stress is highest among all 21 Arabidopsis Hsfs. In this study, it is reported tha...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erm184

    authors: Ogawa D,Yamaguchi K,Nishiuchi T

    更新日期:2007-01-01 00:00:00

  • The climacteric-like behaviour of young, mature and wounded citrus leaves.

    abstract::Although leaves and other vegetative tissues are generally considered as non-climacteric, citrus leaves show a climacteric system II behaviour after detachment. Upon harvest, young, fully expanded 'Valencia' orange (Citrus sinensis) leaves ( approximately 60-d-old) exhibited two phases of ethylene production. The firs...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/eri137

    authors: Katz E,Riov J,Weiss D,Goldschmidt EE

    更新日期:2005-05-01 00:00:00

  • Root architecture simulation improves the inference from seedling root phenotyping towards mature root systems.

    abstract::Root phenotyping provides trait information for plant breeding. A shortcoming of high-throughput root phenotyping is the limitation to seedling plants and failure to make inferences on mature root systems. We suggest root system architecture (RSA) models to predict mature root traits and overcome the inference problem...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erw494

    authors: Zhao J,Bodner G,Rewald B,Leitner D,Nagel KA,Nakhforoosh A

    更新日期:2017-02-01 00:00:00

  • FIT, a regulatory hub for iron deficiency and stress signaling in roots, and FIT-dependent and -independent gene signatures.

    abstract::Iron (Fe) is vital for plant growth. Plants balance the beneficial and toxic effects of this micronutrient, and tightly control Fe uptake and allocation. Here, we review the role of the basic helix-loop-helix (bHLH) transcription factor FIT (FER-LIKE FE DEFICIENCY-INDUCED TRANSCRIPTION FACTOR) in Fe acquisition. FIT i...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/eraa012

    authors: Schwarz B,Bauer P

    更新日期:2020-03-12 00:00:00

  • Making sense of the metabolome using evolutionary computation: seeing the wood with the trees.

    abstract::One should perhaps start off by asking the question, 'But what wood is it we want to see?' There are so many trees that make up the wood; within a post-genomics context, genes, transcripts, proteins, and metabolites are the more tangible ones. Rather than studying these components in isolation, a more holistic approac...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章,评审

    doi:10.1093/jxb/eri043

    authors: Goodacre R

    更新日期:2005-01-01 00:00:00

  • The role of monovalent cation transporters in plant responses to salinity.

    abstract::Exposure to high ambient levels of NaCl affects plant water relations and creates ionic stress in the form of the cellular accumulation of Cl- and, in particular, Na+ ions. However, salt stress also impacts heavily on the homeostasis of other ions such as Ca2+, K+, and NO(3)(-) and therefore requires insights into how...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erj001

    authors: Maathuis FJ

    更新日期:2006-01-01 00:00:00

  • Tricho- and atrichoblast cell files show distinct PIN2 auxin efflux carrier exploitations and are jointly required for defined auxin-dependent root organ growth.

    abstract::The phytohormone auxin is a vital growth regulator in plants. In the root epidermis auxin steers root organ growth. However, the mechanisms that allow adjacent tissues to integrate growth are largely unknown. Here, the focus is on neighbouring epidermal root tissues to assess the integration of auxin-related growth re...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erv282

    authors: Löfke C,Scheuring D,Dünser K,Schöller M,Luschnig C,Kleine-Vehn J

    更新日期:2015-08-01 00:00:00

  • Virus-induced gene silencing of Arabidopsis thaliana gene homologues in wheat identifies genes conferring improved drought tolerance.

    abstract::In a non-model staple crop like wheat (Triticum aestivumI L.), functional validation of potential drought stress responsive genes identified in Arabidopsis could provide gene targets for breeding. Virus-induced gene silencing (VIGS) of genes of interest can overcome the inherent problems of polyploidy and limited tran...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/ert003

    authors: Manmathan H,Shaner D,Snelling J,Tisserat N,Lapitan N

    更新日期:2013-03-01 00:00:00

  • Uptake of sodium in protoplasts of salt-sensitive and salt-tolerant cultivars of rice, Oryza sativa L. determined by the fluorescent dye SBFI.

    abstract::In this study, the uptake of Na+ into the cytosol of rice (Oryza sativa L. cvs Pokkali and BRRI Dhan29) protoplasts was measured using the acetoxy methyl ester of the fluorescent sodium-binding benzofuran isopthalate, SBFI-AM, and fluorescence microscopy. By means of inhibitor analyses the mechanisms for uptake and se...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/eri312

    authors: Kader MA,Lindberg S

    更新日期:2005-12-01 00:00:00

  • Cross-talk between reactive oxygen species and polyamines in regulation of ion transport across the plasma membrane: implications for plant adaptive responses.

    abstract::Many stresses are associated with increased accumulation of reactive oxygen species (ROS) and polyamines (PAs). PAs act as ROS scavengers, but export of putrescine and/or PAs to the apoplast and their catabolization by amine oxidases gives rise to H2O2 and other ROS, including hydroxyl radicals ((•)OH). PA catabolizat...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章,评审

    doi:10.1093/jxb/ert423

    authors: Pottosin I,Velarde-Buendía AM,Bose J,Zepeda-Jazo I,Shabala S,Dobrovinskaya O

    更新日期:2014-03-01 00:00:00

  • CIRCADIAN CLOCK ASSOCIATED 1 and ATAF2 differentially suppress cytochrome P450-mediated brassinosteroid inactivation.

    abstract::Brassinosteroids (BRs) are a group of steroid hormones regulating plant growth and development. Since BRs do not undergo transport among plant tissues, their metabolism is tightly regulated by transcription factors (TFs) and feedback loops. BAS1 (CYP734A1, formerly CYP72B1) and SOB7 (CYP72C1) are two BR-inactivating c...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erz468

    authors: Peng H,Neff MM

    更新日期:2020-01-23 00:00:00

  • Fine mapping of a quantitative trait locus for spikelet number per panicle in a new plant type rice and evaluation of a near-isogenic line for grain productivity.

    abstract::Total spikelet number per panicle (TSN) is one of the determinants of grain productivity in rice (Oryza sativa L.). In this study, we attempted to detect quantitative trait loci (QTLs) for TSN in the introgression lines with high TSN, derived from the cross of Indica Group variety IR 64 with new plant type lines. Two ...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erx128

    authors: Sasaki K,Fujita D,Koide Y,Lumanglas PD,Gannaban RB,Tagle AG,Obara M,Fukuta Y,Kobayashi N,Ishimaru T

    更新日期:2017-05-17 00:00:00

  • miR168 influences phase transition, leaf epinasty, and fruit development via SlAGO1s in tomato.

    abstract::In Arabidopsis thaliana, Argonaute1 (AGO1) interacts with miR168 to modulate the small RNA regulatory pathway. However, the underlying mechanism of regulation and relationship between AGO1 and miR168 is poorly understood in the cash crop Solanum lycopersicum (tomato). We previously found that SlAGO1A and SlAGO1B were ...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/eru387

    authors: Xian Z,Huang W,Yang Y,Tang N,Zhang C,Ren M,Li Z

    更新日期:2014-12-01 00:00:00

  • The rate of CO(2) assimilation controls the expression and activity of glutamine synthetase through sugar formation in sunflower (Helianthus annuus L) leaves.

    abstract::The expression and activity of glutamine synthetase (GS, EC 6.3.1.2) were examined in relation to the rate of CO2 assimilation in sunflower (Helianthus annuus L.) leaves. Intact plants were kept in the dark for 72 h and subsequently exposed to light under different atmospheric CO2 concentrations (100, 400 and 1200 mic...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erh017

    authors: Larios B,Aguera E,Cabello P,Maldonado JM,de la Haba P

    更新日期:2004-01-01 00:00:00

  • FIE, a nuclear PRC2 protein, forms cytoplasmic complexes in Arabidopsis thaliana.

    abstract::Polycomb group (PcG) proteins are evolutionarily conserved chromatin modifiers that regulate developmental pathways in plants. PcGs form nuclear multi-subunit Polycomb Repressive Complexes (PRCs). The PRC2 complex mediates gene repression via methylation of lysine 27 on histone H3, which consequently leads to chromati...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erw373

    authors: Oliva M,Butenko Y,Hsieh TF,Hakim O,Katz A,Smorodinsky NI,Michaeli D,Fischer RL,Ohad N

    更新日期:2016-11-01 00:00:00

  • Long-term in vitro culture of grape berries and its application to assess the effects of sugar supply on anthocyanin accumulation.

    abstract::Grape berry development and ripening are under complex regulation by the nutrients, hormones, and environment cues sensed by the berry. However, the biochemical and molecular mechanisms underlying these types of regulation are poorly understood. A simplified but realistic model system that enables fruit growth conditi...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/ert489

    authors: Dai ZW,Meddar M,Renaud C,Merlin I,Hilbert G,Delrot S,Gomès E

    更新日期:2014-08-01 00:00:00

  • Re-evaluation of the ethylene-dependent and -independent pathways in the regulation of floral and organ abscission.

    abstract::Abscission is a developmental process with important implications for agricultural practices. Ethylene has long been considered as a key regulator of the abscission process. The existence of an ethylene-independent abscission pathway, controlled by the complex of INFLORESCENCE DEFICIENT IN ABSCISSION (IDA) peptide and...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章,评审

    doi:10.1093/jxb/erz038

    authors: Meir S,Philosoph-Hadas S,Riov J,Tucker ML,Patterson SE,Roberts JA

    更新日期:2019-03-11 00:00:00

  • Enhancing trehalose biosynthesis improves yield potential in marker-free transgenic rice under drought, saline, and sodic conditions.

    abstract::Edaphic factors such as salinity, sodicity, and drought adversely affect crop productivity, either alone or in combination. Despite soil sodicity being reported as an increasing problem worldwide, limited efforts have been made to address this issue. In the present study, we aimed to generate rice with tolerance to so...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erz462

    authors: Joshi R,Sahoo KK,Singh AK,Anwar K,Pundir P,Gautam RK,Krishnamurthy SL,Sopory SK,Pareek A,Singla-Pareek SL

    更新日期:2020-01-07 00:00:00

  • Leaf-atmosphere NH(3) exchange of white clover (Trifolium repens L.) in relation to mineral N nutrition and symbiotic N(2) fixation.

    abstract::Plant-atmosphere NH(3) exchange was studied in white clover (Trifolium repens L. cv. Seminole) growing in nutrient solution containing 0 (N(2) based), 0.5 (low N) or 4.5 (high N) mM NO(3)(-). The aim was to show whether the NH(3) exchange potential is influenced by the proportion of N(2) fixation relative to NO(3)(-) ...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:

    authors: Herrmann B,Mattsson M,Fuhrer J,Schjoerring JK

    更新日期:2002-01-01 00:00:00

  • Wing loading, not terminal velocity, is the best parameter to predict capacity of diaspores for secondary wind dispersal.

    abstract::Lift-off velocity may be the most useful surrogate to measure the secondary dispersal capacity of diaspores. However, the most important diaspore attribute determining diaspore lift-off velocity is unclear. Furthermore, it is not known whether terminal velocity used to characterize the primary dispersal capacity of di...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/eraa170

    authors: Liang W,Liu Z,Liu M,Qin X,Baskin CC,Baskin JM,Xin Z,Wang Z,Su Z,Zhou Q

    更新日期:2020-07-06 00:00:00

  • Seed dormancy cycling and the regulation of dormancy mechanisms to time germination in variable field environments.

    abstract::Many molecular mechanisms that regulate dormancy have been identified individually in controlled laboratory studies. However, little is known about how the seed employs this complex suite of mechanisms during dormancy cycling in the variable environment of the soil seed bank. Nevertheless, this behaviour is essential ...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章,评审

    doi:10.1093/jxb/erw477

    authors: Finch-Savage WE,Footitt S

    更新日期:2017-02-01 00:00:00

  • Physical effects of soil drying on roots and crop growth.

    abstract::The nature and effect of the stresses on root growth in crops subject to drying is reviewed. Drought is a complex stress, impacting on plant growth in a number of interacting ways. In response, there are a number of ways in which the growing plant is able to adapt to or alleviate these stresses. It is suggested that t...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章,评审

    doi:10.1093/jxb/erp200

    authors: Whitmore AP,Whalley WR

    更新日期:2009-01-01 00:00:00

  • Analysis of potential redundancy among Arabidopsis 6-phosphogluconolactonase isoforms in peroxisomes.

    abstract::Recent work revealed that PGD2, an Arabidopsis 6-phosphogluconate dehydrogenase (6-PGD) catalysing the third step of the oxidative pentose-phosphate pathway (OPPP) in peroxisomes, is essential during fertilization. Earlier studies on the second step, catalysed by PGL3, a dually targeted Arabidopsis 6-phosphogluconolac...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erz473

    authors: Lansing H,Doering L,Fischer K,Baune MC,Schaewen AV

    更新日期:2020-01-23 00:00:00

  • Root-ABA1 QTL affects root lodging, grain yield, and other agronomic traits in maize grown under well-watered and water-stressed conditions.

    abstract::A major QTL affecting root traits and leaf ABA concentration was identified in maize (Zea mays L.) and named root-ABA1. For this QTL, back-cross-derived lines (BDLs) homozygous either for the (+) or for the (-) allele increasing or decreasing, respectively, root size and leaf ABA concentration, were developed. This st...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erl161

    authors: Landi P,Sanguineti MC,Liu C,Li Y,Wang TY,Giuliani S,Bellotti M,Salvi S,Tuberosa R

    更新日期:2007-01-01 00:00:00

  • Antagonistic peptide technology for functional dissection of CLE peptides revisited.

    abstract::In the Arabidopsis thaliana genome, over 1000 putative genes encoding small, presumably secreted, signalling peptides can be recognized. However, a major obstacle in identifying the function of genes encoding small signalling peptides is the limited number of available loss-of-function mutants. To overcome this, a pro...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erv284

    authors: Czyzewicz N,Wildhagen M,Cattaneo P,Stahl Y,Pinto KG,Aalen RB,Butenko MA,Simon R,Hardtke CS,De Smet I

    更新日期:2015-08-01 00:00:00

  • Mechanism of phytohormone involvement in feedback regulation of cotton leaf senescence induced by potassium deficiency.

    abstract::To elucidate the phytohormonal basis of the feedback regulation of leaf senescence induced by potassium (K) deficiency in cotton (Gossypium hirsutum L.), two cultivars contrasting in sensitivity to K deficiency were self- and reciprocally grafted hypocotyl-to-hypocotyl, using standard grafting (one scion grafted onto ...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/ers238

    authors: Wang Y,Li B,Du M,Eneji AE,Wang B,Duan L,Li Z,Tian X

    更新日期:2012-10-01 00:00:00

  • HPS4/SABRE regulates plant responses to phosphate starvation through antagonistic interaction with ethylene signalling.

    abstract::The phytohormone ethylene plays important roles in regulating plant responses to phosphate (Pi) starvation. To date, however, no molecular components have been identified that interact with ethylene signalling in regulating such responses. In this work, an Arabidopsis mutant, hps4, was characterized that exhibits enha...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/ers131

    authors: Yu H,Luo N,Sun L,Liu D

    更新日期:2012-07-01 00:00:00

  • Gain-of-function analysis of poplar CLE genes in Arabidopsis by exogenous application and over-expression assays.

    abstract::Among 50 CLE gene family members in the Populus trichocarpa genome, three and six PtCLE genes encode a CLE motif sequence highly homologous to Arabidopsis CLV3 and TDIF peptides, respectively, which potentially make them functional equivalents. To test and compare their biological activity, we first chemically synthes...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erw045

    authors: Liu Y,Yang S,Song Y,Men S,Wang J

    更新日期:2016-04-01 00:00:00