Abstract:
:In a non-model staple crop like wheat (Triticum aestivumI L.), functional validation of potential drought stress responsive genes identified in Arabidopsis could provide gene targets for breeding. Virus-induced gene silencing (VIGS) of genes of interest can overcome the inherent problems of polyploidy and limited transformation potential that hamper functional validation studies in wheat. In this study, three potential candidate genes shown to be involved in abiotic stress response pathways in Arabidopsis thaliana were selected for VIGS experiments in wheat. These include Era1 (enhanced response to abscisic acid), Cyp707a (ABA 8'-hydroxylase), and Sal1 (inositol polyphosphate 1-phosphatase). Gene homologues for these three genes were identified in wheat and cloned in the viral vector barley stripe mosaic virus (BSMV) in the antisense direction, followed by rub inoculation of BSMV viral RNA transcripts onto wheat plants. Quantitative real-time PCR showed that VIGS-treated wheat plants had significant reductions in target gene transcripts. When VIGS-treated plants generated for Era1 and Sal1 were subjected to limiting water conditions, they showed increased relative water content, improved water use efficiency, reduced gas exchange, and better vigour compared to water-stressed control plants inoculated with RNA from the empty viral vector (BSMV0). In comparison, the Cyp707a-silenced plants showed no improvement over BSMV0-inoculated plants under limited water condition. These results indicate that Era1 and Sal1 play important roles in conferring drought tolerance in wheat. Other traits affected by Era1 silencing were also studied. Delayed seed germination in Era1-silenced plants suggests this gene may be a useful target for developing resistance to pre-harvest sprouting.
journal_name
J Exp Botjournal_title
Journal of experimental botanyauthors
Manmathan H,Shaner D,Snelling J,Tisserat N,Lapitan Ndoi
10.1093/jxb/ert003subject
Has Abstractpub_date
2013-03-01 00:00:00pages
1381-92issue
5eissn
0022-0957issn
1460-2431pii
ert003journal_volume
64pub_type
杂志文章abstract::Although protein phosphorylation is integral to the regulation of protein function in diverse biological responses, relatively little is currently known about the rules that govern phosphorylation in plants. This review will discuss how the data acquired by evolving phosphoproteomic methods are beginning to fill the g...
journal_title:Journal of experimental botany
pub_type: 杂志文章,评审
doi:10.1093/jxb/erj126
更新日期:2006-01-01 00:00:00
abstract::The effect of iron (Fe) and sulphur (S) deprivation on sulphate uptake and assimilation pathways was investigated in durum wheat by analysing the expression of genes coding for major transporters and enzymes involved in sulphate assimilation and reduction: high-affinity sulphate transporters (TdSultr1.1 and TdSultr1.3...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/ert027
更新日期:2013-04-01 00:00:00
abstract::MicroRNAs (miRNAs) are important transcriptional and post-transcriptional modulators of gene expression that play crucial roles in the responses to diverse stresses. To explore jasmonic acid (JA)-dependent miRNA-mediated regulatory networks that are responsive to root-knot nematode (RKN), two small RNA libraries were ...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erv238
更新日期:2015-08-01 00:00:00
abstract::The absence of a terminal flower in inflorescences ('open inflorescences') is currently explained by the maintenance of putative stem-cells in the central zone (CZ) of the inflorescence meristem (IM) governed by the CLAVATA-WUSCHEL regulatory loop. Disruption of this regulatory pathway, as in Arabidopsis TERMINAL FLOW...
journal_title:Journal of experimental botany
pub_type: 杂志文章,评审
doi:10.1093/jxb/erq262
更新日期:2011-01-01 00:00:00
abstract::Rates of photosynthesis can be lower in plants grown under conditions of high leaf-to-air vapour pressure difference (VPD) than under low VPD. Leaf phenotype plasticity is a primary factor determining photosynthetic responses to environmental stimuli. However, it remains unclear how changes in leaf anatomical traits d...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erz238
更新日期:2019-09-24 00:00:00
abstract::Short-chain esters contribute to the blend of volatiles that define the strawberry aroma. The last step in their biosynthesis involves an alcohol acyltransferase that catalyses the esterification of an acyl moiety of acyl-CoA with an alcohol. This study identified a novel strawberry alcohol acyltransferase gene (FaAAT...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/ers120
更新日期:2012-06-01 00:00:00
abstract::Jasmonates are signaling compounds that regulate plant responses to stress. Jasmonic acid (JA) is the direct precursor of the bioactive plant hormone JA-isoleucine (JA-Ile), the ligand of the CORONATINE INSENSITIVE 1-jasmonate ZIM-domain (COI1-JAZ) co-receptor complex. JA, its methyl ester, and three furanonyl esters ...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/ery114
更新日期:2018-05-25 00:00:00
abstract::Plants respond to many environmental changes by rapidly adjusting their hydraulic conductivity and transpiration rate, thereby optimizing water-use efficiency and preventing damage due to low water potential. A multiple-load-cell apparatus, time-series analysis of the measured data, and residual low-pass filtering met...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erq168
更新日期:2010-07-01 00:00:00
abstract::When plants encounter compacted soil, stomatal closure occurs and shoot growth slows. These responses occur in the absence of detectable changes in foliar water status. The use of genotypes with a reduced capacity to synthesize either ABA or ethylene has provided convincing evidence that ABA is responsible for providi...
journal_title:Journal of experimental botany
pub_type: 杂志文章,评审
doi:
更新日期:2002-01-01 00:00:00
abstract::Seed size is an important component of grain yield and a key determinant trait for crop domestication. The Krug Yellow Dent long-term selection experiment for large and small seed provides a valuable resource to dissect genetic and phenotypic changes affecting seed size within a common genetic background. In this stud...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erw006
更新日期:2016-03-01 00:00:00
abstract::Plant stress studies are more and more based on gene expression. The analysis of gene expression requires sensitive, precise, and reproducible measurements for specific mRNA sequences. Real-time RT-PCR is at present the most sensitive method for the detection of low abundance mRNA. To avoid bias, real-time RT-PCR is r...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/eri285
更新日期:2005-11-01 00:00:00
abstract::Plant mitochondrial respiration involves operation of various alternate pathways. These pathways participate, both directly and indirectly, in the maintenance of mitochondrial functions though they do not contribute to energy production being uncoupled from the generation of an electrochemical gradient across the mito...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/eraa510
更新日期:2020-11-27 00:00:00
abstract::Ergosterol (a fungal membrane component) was shown to induce transient influx of protons and membrane hyperpolarization in cotyledonary cells of Mimosa pudica L. By contrast, chitosan (a fungal wall component with known elicitor properties) triggered membrane depolarization. In the processes induced by ergosterol, a s...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erj090
更新日期:2006-01-01 00:00:00
abstract::Plants as photoautotrophic organisms are able to produce the carbohydrates they require and have developed mechanisms to co-ordinate carbohydrate production and its metabolism. Carbohydrate-derived signals regulate the expression of genes involved in both photosynthesis and metabolism, and control carbohydrate partiti...
journal_title:Journal of experimental botany
pub_type: 杂志文章,评审
doi:10.1093/jxb/erg054
更新日期:2003-01-01 00:00:00
abstract::The two-fold purpose of this work is, first, to review current hypotheses for multiple paths of sugar-sensing in an oxygen-responsive context, and second, to present evidence for the extent of sugar/oxygen overlap regulating genes for sucrose and ethanol metabolism. Current data indicate that sugar signals in plants m...
journal_title:Journal of experimental botany
pub_type: 杂志文章,评审
doi:10.1093/jexbot/51.suppl_1.417
更新日期:2000-02-01 00:00:00
abstract::HYL1 is an important regulator of microRNA (miRNA) biogenesis. A loss-of-function mutation of HYL1 causes the reduced accumulation of some miRNAs but fails to display the miRNA-deficient phenotypes of these miRNAs. In Arabidopsis, miR156 mediates phase transition through repression of SQUAMOSA PROMOTER-BINDING PROTEIN...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/err465
更新日期:2012-04-01 00:00:00
abstract::Classical and recently found phytohormones play an important role in plant growth and development, but plants additionally control these processes through small signalling peptides. Over 1000 potential small signalling peptide sequences are present in the Arabidopsis genome. However, to date, a mere handful of small s...
journal_title:Journal of experimental botany
pub_type: 杂志文章,评审
doi:10.1093/jxb/ert283
更新日期:2013-12-01 00:00:00
abstract::In planta, a vital regulatory complex, MYB-basic helix-loop-helix (bHLH)-WD40 (MBW), is involved in trichome development and synthesis of anthocyanin and proanthocyanin in Arabidopsis. Usually, WD40 proteins provide a scaffold for protein-protein interaction between MYB and bHLH proteins. Members of subgroup 9 of the ...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/eraa104
更新日期:2020-06-22 00:00:00
abstract::Phi thickenings are specialized secondary walls found in root cortical cells. Despite their widespread occurrence throughout the plant kingdom, these specialized thickenings remain poorly understood. First identified by Van Tieghem in 1871, phi thickenings are a lignified and thickened cell wall band that is deposited...
journal_title:Journal of experimental botany
pub_type: 杂志文章,评审
doi:10.1093/jxb/erz240
更新日期:2019-09-24 00:00:00
abstract::In order to understand the role of cytosolic antioxidant enzymes in drought stress protection, transgenic tobacco (Nicotiana tabacum cv. Xanthi) plants overexpressing cytosolic Cu/Zn-superoxide dismutase (cytsod) (EC 1.15.1.1) or ascorbate peroxidase (cytapx) (EC 1.11.1.1) alone, or in combination, were produced and t...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erq432
更新日期:2011-05-01 00:00:00
abstract::In photosynthetic tissues of the CAM plant pineapple (Ananas comosus), storage of soluble sugars in the central vacuole during the daytime and their remobilization at night is required to provide carbon skeletons for nocturnal CO(2) fixation. However, soluble sugars produced photosynthetically must also be exported to...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/ern077
更新日期:2008-01-01 00:00:00
abstract::Silicon (Si) is known to alleviate manganese (Mn) toxicity in a number of plant species; however, the mechanisms responsible for this effect are poorly understood. Here, we investigated the interaction between Si and Mn in rice (Oryza sativa) by using a mutant defective in Si uptake. Silicon alleviated Mn toxicity in ...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erv545
更新日期:2016-03-01 00:00:00
abstract::Reactive oxygen species (ROS) play a key role in the regulation of many biological processes in plants. Nonetheless, they are considered highly reactive and toxic to cells. Owing to their toxicity, as well as their important role in signaling, the level of ROS in cells needs to be tightly regulated. The ROS gene netwo...
journal_title:Journal of experimental botany
pub_type: 杂志文章,评审
doi:10.1093/jxb/erw382
更新日期:2016-11-01 00:00:00
abstract::The phloem is a well-known target of sucking and piercing insects that utilize the transported fluid as their major nutrient source. In addition to small molecules like sugars and amino acids, phloem sap of higher land plants contains proteins that can accumulate up to high concentrations. Although the knowledge about...
journal_title:Journal of experimental botany
pub_type: 杂志文章,评审
doi:10.1093/jxb/erj087
更新日期:2006-01-01 00:00:00
abstract::Plant cell elongation depends on the physical properties of the primary cell wall. Because xyloglucan endotransglycosylases (XETs) are enzymes that mediate cleavage and rejoining of the beta(1-4)-XG backbone of primary cell wall, they are potentially involved in cell elongation. In this paper, the growth of the barley...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:
更新日期:2000-05-01 00:00:00
abstract::Low temperature affects seed germination in plants, and low-temperature germination (LTG) is an important agronomic trait. Natural variation of LTG has been reported in rice, but the molecular basis for this variation is largely unknown. Here we report the phenotypic analysis of LTG in 187 rice natural accessions and ...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erx413
更新日期:2018-01-23 00:00:00
abstract::To study the effect of root-zone pH on characteristic responses of NH4+ -fed plants, soybeans (Glycine max¿L.¿ Merr. cv. Ransom) were grown in flowing solution culture for 21 d on four sources of N (1.0 mol m-3 NO3-, 0.67 mol m-3 NO3- plus 0.33 mol m-3 NH4+, 0.33 mol m-3 NO3- plus 0.67 mol m-3 NH4+, and 1.0 mol m-3 NH...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/42.2.189
更新日期:1991-02-01 00:00:00
abstract::At least seven isoforms (PsABI3-1 to PsABI3-7) of a putative, pea ABI3-like factor, originated by alternative splicing, have been identified after cDNA cloning. A similar variability had previously only been described for monocot genes. The full-length isoform, PsABI3-1, contains the typical N-terminal acidic domains ...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erp038
更新日期:2009-01-01 00:00:00
abstract::The productivity of sorghum is mainly determined by agronomically important traits. The genetic bases of these traits have historically been dissected and analysed through quantitative trait locus (QTL) mapping based on linkage maps with low-throughput molecular markers, which is one of the factors that hinder precise...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/ers205
更新日期:2012-09-01 00:00:00
abstract::The rise in atmospheric [CO(2)] is associated with increasing air temperature. However, studies on plant responses to interactive effects of [CO(2)] and temperature are limited, particularly for leaf structural attributes. In this study, Eucalyptus saligna plants were grown in sun-lit glasshouses differing in [CO(2)] ...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/ers231
更新日期:2012-10-01 00:00:00