Gain-of-function analysis of poplar CLE genes in Arabidopsis by exogenous application and over-expression assays.

Abstract:

:Among 50 CLE gene family members in the Populus trichocarpa genome, three and six PtCLE genes encode a CLE motif sequence highly homologous to Arabidopsis CLV3 and TDIF peptides, respectively, which potentially make them functional equivalents. To test and compare their biological activity, we first chemically synthesized each dodecapeptide and analysed itsi n vitro bioactivity on Arabidopsis seedlings. Similarly, but to a different extent, three types of poplar CLV3-related peptides caused root meristem consumption, phyllotaxis disorder, anthocyanin accumulation and failure to enter the bolting stage. In comparison, application of two poplar TDIF-related peptides led to root length promotion in a dose-dependent manner with an even stronger effect observed for poplar TDIF-like peptide than TDIF. Next, we constructed CaMV35S:PtCLE transgenic plants for each of the nine PtCLE genes. Phenotypic abnormalities exemplified by arrested shoot apical meristem and abnormal flower structure were found to be more dominant and severe in 35S:PtCLV3 and 35S:PtCLV3-like2 lines than in the 35S:PtCLV3-like line. Disordered vasculature was detected in both stem and hypocotyl cross-sections in Arabidopsis plants over-expressing poplar TDIF-related genes with the most defective vascular patterning observed for TDIF2 and two TDIF-like genes. Phenotypic difference consistently observed in peptide application assay and transgenic analysis indicated the functional diversity of nine poplar PtCLE genes under investigation. This work represents the first report on the functional analysis of CLE genes in a tree species and constitutes a basis for further study of the CLE peptide signalling pathway in tree development.

journal_name

J Exp Bot

authors

Liu Y,Yang S,Song Y,Men S,Wang J

doi

10.1093/jxb/erw045

subject

Has Abstract

pub_date

2016-04-01 00:00:00

pages

2309-24

issue

8

eissn

0022-0957

issn

1460-2431

pii

erw045

journal_volume

67

pub_type

杂志文章
  • Ionic currents and ion fluxes in Neurospora crassa hyphae.

    abstract::Voltage dependence of ionic currents and ion fluxes in a walled, turgor-regulating cell were measured in Neurospora crassa. The hyphal morphology of the model organism Neurospora simplifies cable analysis of ionic currents to determine current density for quantitative comparisons with ion fluxes. The ion fluxes were m...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erm204

    authors: Lew RR

    更新日期:2007-01-01 00:00:00

  • ABC transporter OsABCG18 controls the shootward transport of cytokinins and grain yield in rice.

    abstract::Cytokinins are one of the most important phytohormones and play essential roles in multiple life processes in planta. Root-derived cytokinins are transported to the shoots via long-distance transport. The mechanisms of long-distance transport of root-derived cytokinins remain to be demonstrated. In this study, we repo...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erz382

    authors: Zhao J,Yu N,Ju M,Fan B,Zhang Y,Zhu E,Zhang M,Zhang K

    更新日期:2019-11-18 00:00:00

  • Effects of temperature on the cuticular transpiration barrier of two desert plants with water-spender and water-saver strategies.

    abstract::The efficacy of the cuticular transpiration barrier and its resistance to elevated temperatures are significantly higher in a typical water-saver than in a water-spender plant growing in hot desert. ...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erz018

    authors: Bueno A,Alfarhan A,Arand K,Burghardt M,Deininger AC,Hedrich R,Leide J,Seufert P,Staiger S,Riederer M

    更新日期:2019-03-11 00:00:00

  • Foxtail mosaic virus-induced flowering assays in monocot crops.

    abstract::Virus-induced flowering (VIF) exploits RNA or DNA viruses to express flowering time genes to induce flowering in plants. Such plant virus-based tools have recently attracted widespread attention for their fundamental and applied uses in flowering physiology and in accelerating breeding in dicotyledonous crops and wood...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/eraa080

    authors: Yuan C,Li H,Qin C,Zhang X,Chen Q,Zhang P,Xu X,He M,Zhang X,Tör M,Xue D,Wang H,Jackson S,He Y,Liu Y,Shi N,Hong Y

    更新日期:2020-05-30 00:00:00

  • Leaf anatomical adaptations have central roles in photosynthetic acclimation to humidity.

    abstract::Rates of photosynthesis can be lower in plants grown under conditions of high leaf-to-air vapour pressure difference (VPD) than under low VPD. Leaf phenotype plasticity is a primary factor determining photosynthetic responses to environmental stimuli. However, it remains unclear how changes in leaf anatomical traits d...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erz238

    authors: Du Q,Liu T,Jiao X,Song X,Zhang J,Li J

    更新日期:2019-09-24 00:00:00

  • The influence of temperature on within-canopy acclimation and variation in leaf photosynthesis: spatial acclimation to microclimate gradients among climatically divergent Acer rubrum L. genotypes.

    abstract::Leaf gas exchange and temperature response were measured to assess temperature acclimation within a tree canopy in climatically contrasting genotypes of Acer rubrum L. Over the course of two 50 d continuous periods, growth temperature was controlled within tree crowns and the steady-state rate of leaf gas exchange was...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erm177

    authors: Bauerle WL,Bowden JD,Wang GG

    更新日期:2007-01-01 00:00:00

  • Differential adaptation of two varieties of common bean to abiotic stress: I. Effects of drought on yield and photosynthesis.

    abstract::The yield of 24 commercial varieties and accessions of common bean (Phaseolus vulgaris) has been determined at different sites in Chile and Bolivia. Statistical analysis was performed in order to characterize whether a particular variety was more or less stable in yield under different environmental conditions. Amongs...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erj062

    authors: Lizana C,Wentworth M,Martinez JP,Villegas D,Meneses R,Murchie EH,Pastenes C,Lercari B,Vernieri P,Horton P,Pinto M

    更新日期:2006-01-01 00:00:00

  • The inverse relationship between solar-induced fluorescence yield and photosynthetic capacity: benefits for field phenotyping.

    abstract::Improving photosynthesis is considered a promising way to increase crop yield to feed a growing population. Realizing this goal requires non-destructive techniques to quantify photosynthetic variation among crop cultivars. Despite existing remote sensing-based approaches, it remains a question whether solar-induced fl...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/eraa537

    authors: Fu P,Meacham-Hensold K,Siebers MH,Bernacchi CJ

    更新日期:2020-12-19 00:00:00

  • My favourite flowering image.

    abstract::I selected my favourite image from a paper by Professor Friedrich Laibach, the founder of Arabidopsis research. His paper from 1951 is the first paper dealing with natural variation for flowering time in this species, a topic many scientists including myself, have followed up and has resulted in large steps forward in...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/err413

    authors: Koornneef M

    更新日期:2013-12-01 00:00:00

  • Down-regulation of a LBD-like gene, OsIG1, leads to occurrence of unusual double ovules and developmental abnormalities of various floral organs and megagametophyte in rice.

    abstract::The indeterminate gametophyte1 (ig1) mutation was first characterized to modulate female gametophyte development in maize (Zea mays). However, the function of its rice orthologue, OsIG1, remains unknown. For this, we first analysed OsIG1 localization from differential tissues in rice. Real-time quantitative PCR (qRT-P...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/eru396

    authors: Zhang J,Tang W,Huang Y,Niu X,Zhao Y,Han Y,Liu Y

    更新日期:2015-01-01 00:00:00

  • Subcellular localization and interactions among rubber particle proteins from Hevea brasiliensis.

    abstract::Natural rubber (polyisoprene) from the rubber tree Hevea brasiliensis is synthesized by specialized cells called laticifers. It is not clear how rubber particles arise, although one hypothesis is that they derive from the endoplasmic reticulum (ER) membrane. Here we cloned the genes encoding four key proteins found in...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erx331

    authors: Brown D,Feeney M,Ahmadi M,Lonoce C,Sajari R,Di Cola A,Frigerio L

    更新日期:2017-11-02 00:00:00

  • Alleviation of rapid, futile ammonium cycling at the plasma membrane by potassium reveals K+-sensitive and -insensitive components of NH4+ transport.

    abstract::Futile plasma membrane cycling of ammonium (NH4+) is characteristic of low-affinity NH4+ transport, and has been proposed to be a critical factor in NH4+ toxicity. Using unidirectional flux analysis with the positron-emitting tracer 13N in intact seedlings of barley (Hordeum vulgare L.), it is shown that rapid, futile...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erm309

    authors: Szczerba MW,Britto DT,Balkos KD,Kronzucker HJ

    更新日期:2008-01-01 00:00:00

  • A genetic relationship between nitrogen use efficiency and seedling root traits in maize as revealed by QTL analysis.

    abstract::That root system architecture (RSA) has an essential role in nitrogen acquisition is expected in maize, but the genetic relationship between RSA and nitrogen use efficiency (NUE) traits remains to be elucidated. Here, the genetic basis of RSA and NUE traits was investigated in maize using a recombination inbred line p...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erv127

    authors: Li P,Chen F,Cai H,Liu J,Pan Q,Liu Z,Gu R,Mi G,Zhang F,Yuan L

    更新日期:2015-06-01 00:00:00

  • The mitochondrial pentatricopeptide repeat protein EMP12 is involved in the splicing of three nad2 introns and seed development in maize.

    abstract::Plant mitochondrial genes contain cis- and trans-group II introns that must be spliced before translation. The mechanism by which these introns are spliced is not well understood. Several families of proteins have been implicated in the intron splicing, of which the pentatricopeptide repeat (PPR) proteins are proposed...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/ery432

    authors: Sun F,Xiu Z,Jiang R,Liu Y,Zhang X,Yang YZ,Li X,Zhang X,Wang Y,Tan BC

    更新日期:2019-02-05 00:00:00

  • Antagonistic action of low-fluence and high-irradiance modes of response of phytochrome on germination and beta-mannanase activity in Datura ferox seeds.

    abstract::Seed germination is often induced by a pulse of red light perceived by phytochrome and cancelled by a subsequent pulse of far-red light. When the pulse of red light is followed by several hours of darkness, a pulse of far-red light is no longer effective and prolonged far-red is necessary to block germination. The aim...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jexbot/51.347.1127

    authors: de Miguel L,Burgin MJ,Casal JJ,Sánchez RA

    更新日期:2000-06-01 00:00:00

  • The kiwifruit lycopene beta-cyclase plays a significant role in carotenoid accumulation in fruit.

    abstract::The composition of carotenoids, along with anthocyanins and chlorophyll, accounts for the distinctive range of colour found in the Actinidia (kiwifruit) species. Lutein and beta-carotene are the most abundant carotenoids found during fruit development, with beta-carotene concentration increasing rapidly during fruit m...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erp218

    authors: Ampomah-Dwamena C,McGhie T,Wibisono R,Montefiori M,Hellens RP,Allan AC

    更新日期:2009-01-01 00:00:00

  • Synthetic biology approaches for improving photosynthesis.

    abstract::The phenomenal increase in agricultural yields that we have witnessed in the last century has slowed down as we approach the limits of selective breeding and optimization of cultivation techniques. To support the yield increase required to feed an ever-growing population, we will have to identify new ways to boost the...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章,评审

    doi:10.1093/jxb/erz029

    authors: Kubis A,Bar-Even A

    更新日期:2019-03-11 00:00:00

  • Induction of glutathione S-transferase genes of Nicotiana benthamiana following infection by Colletotrichum destructivum and C. orbiculare and involvement of one in resistance.

    abstract::Four glutathione S-transferase (GST) genes, NbGSTU1, NbGSTU2, NbGSTU3, and NbGSTF1, were amplified from cDNA of Nicotiana benthamiana leaves infected with Colletotrichum destructivum using primers based on conserved regions of N. tabacum GST sequences. Expression of NbGSTU1 and NbGSTU3 increased progressively during i...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/eri145

    authors: Dean JD,Goodwin PH,Hsiang T

    更新日期:2005-06-01 00:00:00

  • Thrips advisor: exploiting thrips-induced defences to combat pests on crops.

    abstract::Plants have developed diverse defence mechanisms to ward off herbivorous pests. However, agriculture still faces estimated crop yield losses ranging from 25% to 40% annually. These losses arise not only because of direct feeding damage, but also because many pests serve as vectors of plant viruses. Herbivorous thrips ...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章,评审

    doi:10.1093/jxb/ery060

    authors: Steenbergen M,Abd-El-Haliem A,Bleeker P,Dicke M,Escobar-Bravo R,Cheng G,Haring MA,Kant MR,Kappers I,Klinkhamer PGL,Leiss KA,Legarrea S,Macel M,Mouden S,Pieterse CMJ,Sarde SJ,Schuurink RC,De Vos M,Van Wees SCM,Broekg

    更新日期:2018-04-09 00:00:00

  • Effect of P-deficiency on photoassimilate partitioning and rhythmic changes in fruit and stem diameter of tomato (Lycopersicon esculentum) during fruit growth.

    abstract::Tomato (Lycopersicon esculentum) plants were grown in liquid culture inside the greenhouse of Hiroshima University, Japan. At the first fruiting stage, P was withdrawn from the rooting medium for a period of 19 d and its effect was studied on photosynthesis, stomatal conductance, transpiration, partitioning of 13C and...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erg273

    authors: Fujita K,Okada M,Lei K,Ito J,Ohkura K,Adu-Gyamfi JJ,Mohapatra PK

    更新日期:2003-11-01 00:00:00

  • Differences in glycosyltransferase family 61 accompany variation in seed coat mucilage composition in Plantago spp.

    abstract::Xylans are the most abundant non-cellulosic polysaccharide found in plant cell walls. A diverse range of xylan structures influence tissue function during growth and development. Despite the abundance of xylans in nature, details of the genes and biochemical pathways controlling their biosynthesis are lacking. In this...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erw424

    authors: Phan JL,Tucker MR,Khor SF,Shirley N,Lahnstein J,Beahan C,Bacic A,Burton RA

    更新日期:2016-12-01 00:00:00

  • Overexpression of the OsZIP4 zinc transporter confers disarrangement of zinc distribution in rice plants.

    abstract::Zinc (Zn), an essential nutrient in cells, plays a vital role in controlling cellular processes such as growth, development, and differentiation. Although the mechanisms of Zn translocation in rice plants (Oryza sativa) are not fully understood, it has recently received increased interest. OsZIP4 is a Zn transporter t...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erm147

    authors: Ishimaru Y,Masuda H,Suzuki M,Bashir K,Takahashi M,Nakanishi H,Mori S,Nishizawa NK

    更新日期:2007-01-01 00:00:00

  • Phloem sap proteins: their identities and potential roles in the interaction between plants and phloem-feeding insects.

    abstract::The phloem is a well-known target of sucking and piercing insects that utilize the transported fluid as their major nutrient source. In addition to small molecules like sugars and amino acids, phloem sap of higher land plants contains proteins that can accumulate up to high concentrations. Although the knowledge about...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章,评审

    doi:10.1093/jxb/erj087

    authors: Kehr J

    更新日期:2006-01-01 00:00:00

  • News from the PUB: plant U-box type E3 ubiquitin ligases.

    abstract::Plant U-box type E3 ubiquitin ligases (PUBs) are well known for their functions in a variety of stress responses, including immune responses and the adaptation to abiotic stresses. First linked to pollen self-incompatibility, their repertoire of roles has grown to encompass also the regulation of developmental process...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章,评审

    doi:10.1093/jxb/erx411

    authors: Trujillo M

    更新日期:2018-01-23 00:00:00

  • Ferroptosis in plants: triggers, proposed mechanisms and the role of iron in modulating cell death.

    abstract::Regulated cell death plays key roles during essential processes along the plant life cycle. It takes part of specific developmental programs and maintains the organism homeostasis in response to unfavorable environments. Ferroptosis is a recently discovered iron-dependent cell death pathway characterized by the accumu...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/eraa425

    authors: Distéfano AM,López GA,Setzes N,Marchetti F,Cainzos M,Cascallares M,Zabaleta E,Pagnussat GC

    更新日期:2020-09-11 00:00:00

  • The soluble proteome of tobacco Bright Yellow-2 cells undergoing H₂O₂-induced programmed cell death.

    abstract::Plant programmed cell death (PCD) is a genetically controlled process that plays an important role in development and stress responses. Reactive oxygen species (ROS) are key inducers of PCD. The addition of 50 mM H₂O₂ to tobacco Bright Yellow-2 (TBY-2) cell cultures induces PCD. A comparative proteomic analysis of TBY...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/ers031

    authors: Vannini C,Marsoni M,Cantara C,De Pinto MC,Locato V,De Gara L,Bracale M

    更新日期:2012-05-01 00:00:00

  • Cloning and functional comparison of a high-affinity K+ transporter gene PhaHKT1 of salt-tolerant and salt-sensitive reed plants.

    abstract::To understand the mechanisms of ion homeostasis in salt-tolerant and salt-sensitive plants, cDNAs for a high-affinity K(+) transporter PhaHKT1 were isolated from salt-sensitive (Utsunomiya) and salt-tolerant (Nanpi, Enchi) reed plants. A cDNA of Utsunomiya (PhaHKT1-u) contained two insertions in the region correspondi...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erm306

    authors: Takahashi R,Liu S,Takano T

    更新日期:2007-01-01 00:00:00

  • Transcriptional and biochemical analyses of gibberellin expression and content in germinated barley grain.

    abstract::Mobilization of reserves in germinated cereal grains is critical for early seedling vigour, global crop productivity, and hence food security. Gibberellins (GAs) are central to this process. We have developed a spatio-temporal model that describes the multifaceted mechanisms of GA regulation in germinated barley grain...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erz546

    authors: Betts NS,Dockter C,Berkowitz O,Collins HM,Hooi M,Lu Q,Burton RA,Bulone V,Skadhauge B,Whelan J,Fincher GB

    更新日期:2020-03-25 00:00:00

  • Using chromosome introgression lines to map quantitative trait loci for photosynthesis parameters in rice (Oryza sativa L.) leaves under drought and well-watered field conditions.

    abstract::Photosynthesis is fundamental to biomass production, but sensitive to drought. To understand the genetics of leaf photosynthesis, especially under drought, upland rice cv. Haogelao, lowland rice cv. Shennong265, and 94 of their introgression lines (ILs) were studied at flowering and grain filling under drought and wel...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/err292

    authors: Gu J,Yin X,Struik PC,Stomph TJ,Wang H

    更新日期:2012-01-01 00:00:00

  • Influx and accumulation of Cs(+) by the akt1 mutant of Arabidopsis thaliana (L.) Heynh. lacking a dominant K(+) transport system.

    abstract::An extensive literature reports that Cs(+), an environmental contaminant, enters plant cells through K(+) transport systems. Several recently identified plant K(+) transport systems are permeable to Cs(+). Permeation models indicate that most Cs(+) uptake into plant roots under typical soil ionic conditions will be me...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jexbot/52.357.839

    authors: Broadley MR,Escobar-Gutiérrez AJ,Bowen HC,Willey NJ,White PJ

    更新日期:2001-04-01 00:00:00