Cloning and functional comparison of a high-affinity K+ transporter gene PhaHKT1 of salt-tolerant and salt-sensitive reed plants.


:To understand the mechanisms of ion homeostasis in salt-tolerant and salt-sensitive plants, cDNAs for a high-affinity K(+) transporter PhaHKT1 were isolated from salt-sensitive (Utsunomiya) and salt-tolerant (Nanpi, Enchi) reed plants. A cDNA of Utsunomiya (PhaHKT1-u) contained two insertions in the region corresponding to the first and second introns of the PhaHKT1 gene, which resulted in a sequence 141 amino acid residues shorter than that of Nanpi. Expression of PhaHKT1 mRNA was detected in the roots of Nanpi and Enchi plants under K(+) starvation conditions and also under Na(+) treatment conditions, whereas it was only slightly detected in the roots of Utsunomiya plants under each of these conditions. In the upper parts, PhaHKT1 expression was detected in the Utsunomiya plants, and two signals were obtained in the Nanpi and Enchi plants under all and K(+) starvation conditions, respectively. Yeasts expressing the PhaHKT1 of Nanpi (PhaHKT1-n) or the PhaHKT1 of Enchi (PhaHKT1-e) grew better in the presence of NaCl than yeast expressing PhaHKT1-u. Furthermore, yeast expressing a chimeric cDNA containing the 5' region of the Utsunomiya gene and the 3' region of the Nanpi gene had partial salt tolerance, and yeast expressing a chimeric cDNA containing the 5' region of the Nanpi gene and the 3' region of the Utsunomiya gene had a reduced ability to take up ions. These results suggest that PhaHKT1 plays an important role in the acquisition of K(+) and maintenance of ion balance under saline conditions.


J Exp Bot


Takahashi R,Liu S,Takano T




Has Abstract


2007-01-01 00:00:00














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    journal_title:Journal of experimental botany

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    authors: Sowiński P,Szczepanik J,Minchin PE

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    abstract::Receptor kinases play important roles in plant growth and development, but only few of them have been functionally characterized in depth. Over the past decade CRINKLY 4 (CR4)-related research has peaked as a result of a newly discovered role of ARABIDOPSIS CR4 (ACR4) in the root. Here, we comprehensively review the a...

    journal_title:Journal of experimental botany

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    authors: Czyzewicz N,Nikonorova N,Meyer MR,Sandal P,Shah S,Vu LD,Gevaert K,Rao AG,De Smet I

    更新日期:2016-08-01 00:00:00

  • Targeting of a polytopic membrane protein to the inner envelope membrane of chloroplasts in vivo involves multiple transmembrane segments.

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    authors: Okawa K,Inoue H,Adachi F,Nakayama K,Ito-Inaba Y,Schnell DJ,Uehara S,Inaba T

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  • Intron-regulated expression of SUVH3, an Arabidopsis Su(var)3-9 homologue.

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    journal_title:Journal of experimental botany

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    authors: Casas-Mollano JA,Lao NT,Kavanagh TA

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    journal_title:Journal of experimental botany

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    authors: Rodriguez-Saona C,Vorsa N,Singh AP,Johnson-Cicalese J,Szendrei Z,Mescher MC,Frost CJ

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    journal_title:Journal of experimental botany

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    authors: Zhang L,Li Y,Lu W,Meng F,Wu CA,Guo X

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    journal_title:Journal of experimental botany

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    journal_title:Journal of experimental botany

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  • KNOX protein KNAT1 regulates fruitlet abscission in litchi by repressing ethylene biosynthetic genes.

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    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Zhao M,Li C,Ma X,Xia R,Chen J,Liu X,Ying P,Peng M,Wang J,Shi CL,Li J

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    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Broadley MR,Bowen HC,Cotterill HL,Hammond JP,Meacham MC,Mead A,White PJ

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    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Lizana C,Wentworth M,Martinez JP,Villegas D,Meneses R,Murchie EH,Pastenes C,Lercari B,Vernieri P,Horton P,Pinto M

    更新日期:2006-01-01 00:00:00

  • Effects of drought stress and subsequent rewatering on photosynthetic and respiratory pathways in Nicotiana sylvestris wild type and the mitochondrial complex I-deficient CMSII mutant.

    abstract::The interaction of photosynthesis and respiration has been studied in vivo under conditions of limited water supply and after consecutive rewatering. The role of the alternative (v(alt)) and cytochrome (v(cyt)) pathways on drought stress-induced suppression of photosynthesis and during photosynthetic recovery was exam...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Galle A,Florez-Sarasa I,Thameur A,de Paepe R,Flexas J,Ribas-Carbo M

    更新日期:2010-03-01 00:00:00