Cloning and functional comparison of a high-affinity K+ transporter gene PhaHKT1 of salt-tolerant and salt-sensitive reed plants.

Abstract:

:To understand the mechanisms of ion homeostasis in salt-tolerant and salt-sensitive plants, cDNAs for a high-affinity K(+) transporter PhaHKT1 were isolated from salt-sensitive (Utsunomiya) and salt-tolerant (Nanpi, Enchi) reed plants. A cDNA of Utsunomiya (PhaHKT1-u) contained two insertions in the region corresponding to the first and second introns of the PhaHKT1 gene, which resulted in a sequence 141 amino acid residues shorter than that of Nanpi. Expression of PhaHKT1 mRNA was detected in the roots of Nanpi and Enchi plants under K(+) starvation conditions and also under Na(+) treatment conditions, whereas it was only slightly detected in the roots of Utsunomiya plants under each of these conditions. In the upper parts, PhaHKT1 expression was detected in the Utsunomiya plants, and two signals were obtained in the Nanpi and Enchi plants under all and K(+) starvation conditions, respectively. Yeasts expressing the PhaHKT1 of Nanpi (PhaHKT1-n) or the PhaHKT1 of Enchi (PhaHKT1-e) grew better in the presence of NaCl than yeast expressing PhaHKT1-u. Furthermore, yeast expressing a chimeric cDNA containing the 5' region of the Utsunomiya gene and the 3' region of the Nanpi gene had partial salt tolerance, and yeast expressing a chimeric cDNA containing the 5' region of the Nanpi gene and the 3' region of the Utsunomiya gene had a reduced ability to take up ions. These results suggest that PhaHKT1 plays an important role in the acquisition of K(+) and maintenance of ion balance under saline conditions.

journal_name

J Exp Bot

authors

Takahashi R,Liu S,Takano T

doi

10.1093/jxb/erm306

subject

Has Abstract

pub_date

2007-01-01 00:00:00

pages

4387-95

issue

15-16

eissn

0022-0957

issn

1460-2431

pii

erm306

journal_volume

58

pub_type

杂志文章
  • Enhancing trehalose biosynthesis improves yield potential in marker-free transgenic rice under drought, saline, and sodic conditions.

    abstract::Edaphic factors such as salinity, sodicity, and drought adversely affect crop productivity, either alone or in combination. Despite soil sodicity being reported as an increasing problem worldwide, limited efforts have been made to address this issue. In the present study, we aimed to generate rice with tolerance to so...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erz462

    authors: Joshi R,Sahoo KK,Singh AK,Anwar K,Pundir P,Gautam RK,Krishnamurthy SL,Sopory SK,Pareek A,Singla-Pareek SL

    更新日期:2020-01-07 00:00:00

  • Architectural and physiological heterogeneity within the synflorescence of the pseudoviviparous grass Poa alpina var. vivipara L.

    abstract::Many biotypes of the northern-hemisphere Arctic-Alpine grass Poa alpina L. reproduce asexually via prolification of the spikelet axis to produce dehiscing shoots. Although capable of photosynthesis, the source-sink characteristics of these synflorescence systems are unknown, including the degree to which plantlets fro...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jexbot/51.351.1705

    authors: Pierce S,Stirling CM,Baxter R

    更新日期:2000-10-01 00:00:00

  • Metabolic analysis of kiwifruit (Actinidia deliciosa) berries from extreme genotypes reveals hallmarks for fruit starch metabolism.

    abstract::Tomato, melon, grape, peach, and strawberry primarily accumulate soluble sugars during fruit development. In contrast, kiwifruit (Actinidia Lindl. spp.) and banana store a large amount of starch that is released as soluble sugars only after the fruit has reached maturity. By integrating metabolites measured by gas chr...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/ert293

    authors: Nardozza S,Boldingh HL,Osorio S,Höhne M,Wohlers M,Gleave AP,MacRae EA,Richardson AC,Atkinson RG,Sulpice R,Fernie AR,Clearwater MJ

    更新日期:2013-11-01 00:00:00

  • Involvement of reactive oxygen species in endosperm cap weakening and embryo elongation growth during lettuce seed germination.

    abstract::Endosperm cap (CAP) weakening and embryo elongation growth are prerequisites for the completion of lettuce seed germination. Although it has been proposed that the cell wall loosening underlying these processes results from an enzymatic mechanism, it is still unclear which enzymes are involved. Here it is shown that r...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/eru167

    authors: Zhang Y,Chen B,Xu Z,Shi Z,Chen S,Huang X,Chen J,Wang X

    更新日期:2014-07-01 00:00:00

  • Co-ordinated gene expression during phases of dormancy release in raspberry (Rubus idaeus L.) buds.

    abstract::Bud break in raspberry (Rubus idaeus L.) is often poor and uneven, with many of the subapical buds remaining in a dormant state. In order to determine the dormancy status of raspberry buds, an empirical measure of bud burst in a growth-permissive environment following exposure to chilling (4 degrees C cold storage) wa...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erl266

    authors: Mazzitelli L,Hancock RD,Haupt S,Walker PG,Pont SD,McNicol J,Cardle L,Morris J,Viola R,Brennan R,Hedley PE,Taylor MA

    更新日期:2007-01-01 00:00:00

  • Effects of simultaneous expression of heterologous genes involved in phytochelatin biosynthesis on thiol content and cadmium accumulation in tobacco plants.

    abstract::Transgenic tobacco (Nicotiana tabacum cv. LA Burley 21) lines expressing three genes encoding enzymes thought to be critical for the efficient production of phytochelatins, (i) serine acetyltransferase (EC 2.3.1.30) involved in the production of O-acetylserine, the cysteine precursor, (ii) gamma-glutamylcysteine synth...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erj176

    authors: Wawrzynski A,Kopera E,Wawrzynska A,Kaminska J,Bal W,Sirko A

    更新日期:2006-01-01 00:00:00

  • Contrasting adaptive strategies to terminal drought-stress gradients in Mediterranean legumes: phenology, productivity, and water relations in wild and domesticated Lupinus luteus L.

    abstract::Our understanding of within-species annual plant adaptation to rainfall gradients is fragmented. Broad-scale ecological applications of Grime's C-S-R triangle are often superficial, while detailed drought physiology tends to be narrow, focusing on elite cultivars. The former lack the detail to explain how plants respo...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/eru006

    authors: Berger JD,Ludwig C

    更新日期:2014-11-01 00:00:00

  • Identification and characterization of Arabidopsis AtNUDX9 as a GDP-d-mannose pyrophosphohydrolase: its involvement in root growth inhibition in response to ammonium.

    abstract::GDP-d-mannose (GDP-d-Man) is an important intermediate in ascorbic acid (AsA) synthesis, cell wall synthesis, protein N-glycosylation, and glycosylphosphatidylinositol-anchoring in plants. Thus, the modulation of intracellular levels of GDP-d-Man could be important for maintaining various cellular processes. Here an A...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erv281

    authors: Tanaka H,Maruta T,Ogawa T,Tanabe N,Tamoi M,Yoshimura K,Shigeoka S

    更新日期:2015-09-01 00:00:00

  • Expression pattern, genomic structure, and promoter analysis of the gene encoding stilbene synthase from Chinese wild Vitis pseudoreticulata.

    abstract::The gene encoding stilbene synthase (STS) plays a central role in many biochemical and physiological actions, and its metabolite resveratrol possesses broad-spectrum resistance to pathogens, as well as diverse pharmacological properties, notably an anticancer effect. Here, we report the expression analysis of the gene...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erq447

    authors: Xu W,Yu Y,Zhou Q,Ding J,Dai L,Xie X,Xu Y,Zhang C,Wang Y

    更新日期:2011-05-01 00:00:00

  • Endosperm development in Brachypodium distachyon.

    abstract::Grain development and its evolution in grasses remains poorly understood, despite cereals being our most important source of food. The grain, for which many grass species have been domesticated, is a single-seeded fruit with prominent and persistent endosperm. Brachypodium distachyon, a small wild grass, is being posi...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erq309

    authors: Opanowicz M,Hands P,Betts D,Parker ML,Toole GA,Mills EN,Doonan JH,Drea S

    更新日期:2011-01-01 00:00:00

  • A new technique for measurement of water permeability of stomatous cuticular membranes isolated from Hedera helix leaves.

    abstract::Transpiration of cuticular membranes isolated from the lower stomatous surface of Hedera helix (ivy) leaves was measured using a novel approach which allowed a distinction to be made between gas phase diffusion (through stomatal pores) and solid phase diffusion (transport through the polymer matrix membrane and cuticu...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erh150

    authors: Santrůcek J,Simánová E,Karbulková J,Simková M,Schreiber L

    更新日期:2004-06-01 00:00:00

  • Photosynthesis at an extreme end of the leaf trait spectrum: how does it relate to high leaf dry mass per area and associated structural parameters?

    abstract::Leaf dry mass per area (LMA) is a composite parameter relating to a suite of structural traits that have the potential to influence photosynthesis. However, the extent to which each of these traits contributes to variation in LMA and photosynthetic rates is not well understood, especially at the high end of the LMA sp...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erq128

    authors: Hassiotou F,Renton M,Ludwig M,Evans JR,Veneklaas EJ

    更新日期:2010-06-01 00:00:00

  • QTL: their place in engineering tolerance of rice to salinity.

    abstract::Secondary salinization and its relationship to irrigation are strong incentives to improve the tolerance of crops to salinity and to drought. Achieving this through the pyramiding of physiological traits (phenotypic selection without knowledge of genotype) is feasible. However, wide application of this approach is lim...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:

    authors: Flowers TJ,Koyama ML,Flowers SA,Sudhakar C,Singh KP,Yeo AR

    更新日期:2000-01-01 00:00:00

  • SPL33, encoding an eEF1A-like protein, negatively regulates cell death and defense responses in rice.

    abstract::Lesion-mimic mutants are useful to dissect programmed cell death and defense-related pathways in plants. Here we identified a new rice lesion-mimic mutant, spotted leaf 33 (spl33) and cloned the causal gene by a map-based cloning strategy. SPL33 encodes a eukaryotic translation elongation factor 1 alpha (eEF1A)-like p...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erx001

    authors: Wang S,Lei C,Wang J,Ma J,Tang S,Wang C,Zhao K,Tian P,Zhang H,Qi C,Cheng Z,Zhang X,Guo X,Liu L,Wu C,Wan J

    更新日期:2017-02-01 00:00:00

  • Developmental and stress regulation of gene expression for a 9-cis-epoxycarotenoid dioxygenase, CstNCED, isolated from Crocus sativus stigmas.

    abstract::Oxidative cleavage of cis-epoxycarotenoids by 9-cis-epoxycarotenoid dioxygenase (NCED) is the critical step in the regulation of abscisic acid (ABA) synthesis in higher plants. ABA has been associated with dormancy and flower senescence, while also regulating plant adaptive responses to various environmental stresses....

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/err293

    authors: Ahrazem O,Rubio-Moraga A,Trapero A,Gómez-Gómez L

    更新日期:2012-01-01 00:00:00

  • Exploitation of genomic sequences in a systematic analysis to access how cyanobacteria sense environmental stress.

    abstract::The perception and subsequent transduction of environmental signals are primary events in the acclimation of living organisms to changes in their environment. Many of the molecular sensors and transducers of environmental stress cannot be identified by traditional and conventional methods. Therefore, the genomic infor...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章,评审

    doi:10.1093/jxb/erj005

    authors: Murata N,Suzuki I

    更新日期:2006-01-01 00:00:00

  • Metabolic and transcriptional transitions in barley glumes reveal a role as transitory resource buffers during endosperm filling.

    abstract::During grain filling in barley (Hordeum vulgare L. cv. Barke) reserves are remobilized from vegetative organs. Glumes represent the vegetative tissues closest to grains, senesce late, and are involved in the conversion of assimilates. To analyse glume development and metabolism related to grain filling, parallel trans...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/eru492

    authors: Kohl S,Hollmann J,Erban A,Kopka J,Riewe D,Weschke W,Weber H

    更新日期:2015-03-01 00:00:00

  • High water availability increases the negative impact of a native hemiparasite on its non-native host.

    abstract::Environmental factors alter the impacts of parasitic plants on their hosts. However, there have been no controlled studies on how water availability modulates stem hemiparasites' effects on hosts. A glasshouse experiment was conducted to investigate the association between the Australian native stem hemiparasite Cassy...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erv548

    authors: Cirocco RM,Facelli JM,Watling JR

    更新日期:2016-03-01 00:00:00

  • Analysis of potential redundancy among Arabidopsis 6-phosphogluconolactonase isoforms in peroxisomes.

    abstract::Recent work revealed that PGD2, an Arabidopsis 6-phosphogluconate dehydrogenase (6-PGD) catalysing the third step of the oxidative pentose-phosphate pathway (OPPP) in peroxisomes, is essential during fertilization. Earlier studies on the second step, catalysed by PGL3, a dually targeted Arabidopsis 6-phosphogluconolac...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erz473

    authors: Lansing H,Doering L,Fischer K,Baune MC,Schaewen AV

    更新日期:2020-01-23 00:00:00

  • The plastid outer envelope protein OEP16 affects metabolic fluxes during ABA-controlled seed development and germination.

    abstract::Previously, the OEP16.1 channel pore in the outer envelope membrane of mature pea (Pisum sativum) chloroplasts in vitro has been characterized to be selective for amino acids. Isolation of OEP16.2, a second OEP16 isoform from pea, in the current study allowed membrane localization and gene expression of OEP16 to be fo...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/err375

    authors: Pudelski B,Schock A,Hoth S,Radchuk R,Weber H,Hofmann J,Sonnewald U,Soll J,Philippar K

    更新日期:2012-03-01 00:00:00

  • Bridging old and new: diversity and evaluation of high iron-associated stress response of rice cultivated in West Africa.

    abstract::Adoption of rice varieties that perform well under high iron-associated (HIA) stress environments can enhance rice production in West Africa. This study reports the genetic characterization of 323 rice accessions and breeding lines cultivated in West Africa using genotyping-by-sequencing and their phenotypic response ...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/eraa182

    authors: Diop B,Wang DR,Drame KN,Gracen V,Tongoona P,Dzidzienyo D,Nartey E,Greenberg AJ,Djiba S,Danquah EY,McCouch SR

    更新日期:2020-07-06 00:00:00

  • Genetic approaches to understanding sugar-response pathways.

    abstract::Plants as photoautotrophic organisms are able to produce the carbohydrates they require and have developed mechanisms to co-ordinate carbohydrate production and its metabolism. Carbohydrate-derived signals regulate the expression of genes involved in both photosynthesis and metabolism, and control carbohydrate partiti...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章,评审

    doi:10.1093/jxb/erg054

    authors: Rook F,Bevan MW

    更新日期:2003-01-01 00:00:00

  • Overexpression of VP, a vacuolar H+-pyrophosphatase gene in wheat (Triticum aestivum L.), improves tobacco plant growth under Pi and N deprivation, high salinity, and drought.

    abstract::Establishing crop cultivars with strong tolerance to P and N deprivation, high salinity, and drought is an effective way to improve crop yield and promote sustainable agriculture worldwide. A vacuolar H+-pyrophosphatase (V-H+-PPase) gene in wheat (TaVP) was functionally characterized in this study. TaVP cDNA is 2586-b...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章,收录出版

    doi:10.1093/jxb/ert442

    authors: Li X,Guo C,Gu J,Duan W,Zhao M,Ma C,Du X,Lu W,Xiao K

    更新日期:2014-02-01 00:00:00

  • Sustained enhancement of photosynthesis in coffee trees grown under free-air CO2 enrichment conditions: disentangling the contributions of stomatal, mesophyll, and biochemical limitations.

    abstract::Coffee (Coffea spp.), a globally traded commodity, is a slow-growing tropical tree species that displays an improved photosynthetic performance when grown under elevated atmospheric CO2 concentrations ([CO2]). To investigate the mechanisms underlying this response, two commercial coffee cultivars (Catuaí and Obatã) we...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erv463

    authors: DaMatta FM,Godoy AG,Menezes-Silva PE,Martins SC,Sanglard LM,Morais LE,Torre-Neto A,Ghini R

    更新日期:2016-01-01 00:00:00

  • A multi-model framework for the Arabidopsis life cycle.

    abstract::Linking our understanding of biological processes at different scales is a major conceptual challenge in biology and aggravated by differences in research methods. Modelling can be a useful approach to consolidating our understanding across traditional research domains. The laboratory model species Arabidopsis is very...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/ery394

    authors: Zardilis A,Hume A,Millar AJ

    更新日期:2019-04-29 00:00:00

  • The AMI1 gene family: indole-3-acetamide hydrolase functions in auxin biosynthesis in plants.

    abstract::Novel genes that function in the conversion of indole-3-acetamide (IAM) into indole-3-acetic acid (IAA), which were previously thought to exist only in the bacterial genome, have been isolated from plants. The finding of the AtAMI1 gene in Arabidopsis thaliana and the NtAMI1 gene in Nicotiana tabacum, which encode ind...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章,评审

    doi:10.1093/jxb/erp292

    authors: Mano Y,Nemoto K,Suzuki M,Seki H,Fujii I,Muranaka T

    更新日期:2010-01-01 00:00:00

  • Differential transcript accumulation in Cicer arietinum L. in response to a chewing insect Helicoverpa armigera and defence regulators correlate with reduced insect performance.

    abstract::Monitoring transcriptional reorganization triggered in response to a particular stress is an essential first step for the functional analysis of genes involved in the process. To characterize Cicer arietinum L. defence responses against Helicoverpa armigera feeding, transcript patterns elicited by both herbivore and m...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/ern111

    authors: Singh A,Singh IK,Verma PK

    更新日期:2008-01-01 00:00:00

  • A comparative study of the expression of serine proteinases in quiescent seeds and in developing Canavalia ensiformis plants.

    abstract::An alkaline proteinase activity is present in quiescent seeds and up to the 24th day of development of Canavalia ensiformis DC (L.) plants. By a simple protocol consisting of cation exchange chromatography, followed by an anion exchange column, a serine proteinase (Q-SP) was purified to homogeneity from quiescent seed...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erl223

    authors: Demartini DR,Wlodawer A,Carlini CR

    更新日期:2007-01-01 00:00:00

  • Interactive effects of elevated CO2, warming, and drought on photosynthesis of Deschampsia flexuosa in a temperate heath ecosystem.

    abstract::Global change factors affect plant carbon uptake in concert. In order to investigate the response directions and potential interactive effects, and to understand the underlying mechanisms, multifactor experiments are needed. The focus of this study was on the photosynthetic response to elevated CO(2) [CO2; free air CO...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/err133

    authors: Albert KR,Ro-Poulsen H,Mikkelsen TN,Michelsen A,van der Linden L,Beier C

    更新日期:2011-08-01 00:00:00

  • Overproduction of SAT and/or OASTL in transgenic plants: a survey of effects.

    abstract::The last steps of cysteine biosynthesis are catalysed by a bi-enzyme complex composed of serine acetyltransferase (SAT) and cysteine synthase, also called O-acetyl-serine (thiol) lyase (OASTL). SAT is responsible for the production of O-acetyl-serine (OAS) from serine and acetyl-coenzyme A, while OASTL catalyses the f...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章,评审

    doi:10.1093/jxb/erh151

    authors: Sirko A,Błaszczyk A,Liszewska F

    更新日期:2004-08-01 00:00:00