Foxtail mosaic virus-induced flowering assays in monocot crops.


:Virus-induced flowering (VIF) exploits RNA or DNA viruses to express flowering time genes to induce flowering in plants. Such plant virus-based tools have recently attracted widespread attention for their fundamental and applied uses in flowering physiology and in accelerating breeding in dicotyledonous crops and woody fruit-trees. We now extend this technology to a monocot grass and a cereal crop. Using a Foxtail mosaic virus (FoMV)-based VIF system, dubbed FoMViF, we showed that expression of florigenic Flowering Locus T (FT) genes can promote early flowering and spikelet development in proso millet, a C4 grass species with potential as a nutritional food and biofuel resource, and in non-vernalized C3 wheat, a major food crop worldwide. Floral and spikelet/grain induction in the two monocot plants was caused by the virally expressed untagged or FLAG-tagged FT orthologs, and the florigenic activity of rice Hd3a was more pronounced than its dicotyledonous counterparts in proso millet. The FoMViF system is easy to use and its efficacy to induce flowering and early spikelet/grain production is high. In addition to proso millet and wheat, we envisage that FoMViF will be also applicable to many economically important monocotyledonous food and biofuel crops.


J Exp Bot


Yuan C,Li H,Qin C,Zhang X,Chen Q,Zhang P,Xu X,He M,Zhang X,Tör M,Xue D,Wang H,Jackson S,He Y,Liu Y,Shi N,Hong Y




Has Abstract


2020-05-30 00:00:00














  • Salinity-induced reduction in root surface area and changes in major root and shoot traits at the phytomer level in wheat.

    abstract::The aim of this study was to investigate the effect of salinity stress on root growth at the phytomer level in wheat to provide novel site-specific understanding of salinity damage in roots. Seedlings of 13 wheat varieties were grown hydroponically. Plants were exposed to three concentrations of NaCl, 0 (control), 50 ...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Robin AH,Matthew C,Uddin MJ,Bayazid KN

    更新日期:2016-06-01 00:00:00

  • Estimating chlorophyll content and photochemical yield of photosystem II (ΦPSII) using solar-induced chlorophyll fluorescence measurements at different growing stages of attached leaves.

    abstract::This paper illustrates the possibility of measuring chlorophyll (Chl) content and Chl fluorescence parameters by the solar-induced Chl fluorescence (SIF) method using the Fraunhofer line depth (FLD) principle, and compares the results with the standard measurement methods. A high-spectral resolution HR2000+ and an ord...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Tubuxin B,Rahimzadeh-Bajgiran P,Ginnan Y,Hosoi F,Omasa K

    更新日期:2015-09-01 00:00:00

  • Soluble methionine enhances accumulation of a 15 kDa zein, a methionine-rich storage protein, in transgenic alfalfa but not in transgenic tobacco plants.

    abstract::With the general aim of elevating the content of the essential amino acid methionine in vegetative tissues of plants, alfalfa (Medicago sativa L.) and tobacco plants, as well as BY2 tobacco suspension cells, were transformed with a beta-zein::3HA gene under the 35S promoter of cauliflower mosaic virus encoding a rumen...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Amira G,Ifat M,Tal A,Hana B,Shmuel G,Rachel A

    更新日期:2005-09-01 00:00:00

  • Nitric oxide signalling in plant interactions with pathogenic fungi and oomycetes.

    abstract::Nitric oxide (NO) and reactive nitrogen species have emerged as crucial signalling and regulatory molecules across all organisms. In plants, fungi and fungi-like oomycetes, NO is involved in the regulation of multiple processes during their growth, development, reproduction, responses to the external environment and b...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Jedelská T,Luhová L,Petřivalský M

    更新日期:2020-12-24 00:00:00

  • The peach (Prunus persica L. Batsch) genome harbours 10 KNOX genes, which are differentially expressed in stem development, and the class 1 KNOPE1 regulates elongation and lignification during primary growth.

    abstract::The KNOTTED-like (KNOX) genes encode homeodomain transcription factors and regulate several processes of plant organ development. The peach (Prunus persica L. Batsch) genome was found to contain 10 KNOX members (KNOPE genes); six of them were experimentally located on the Prunus reference map and the class 1 KNOPE1 wa...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Testone G,Condello E,Verde I,Nicolodi C,Caboni E,Dettori MT,Vendramin E,Bruno L,Bitonti MB,Mele G,Giannino D

    更新日期:2012-09-01 00:00:00

  • Involvement of Arabidopsis phospholipase D δ in regulation of ROS-mediated microtubule organization and stomatal movement upon heat shock.

    abstract::Reactive oxygen species (ROS) are plant metabolic and signaling molecules involved in responses to various external stresses, but the existence of ROS receptors and how plants respond to ROS remain largely unknown. Here we report that the plasma membrane-localized phospholipase D δ (PLDδ) protein is crucial for sensin...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Song P,Jia Q,Chen L,Jin X,Xiao X,Li L,Chen H,Qu Y,Su Y,Zhang W,Zhang Q

    更新日期:2020-10-22 00:00:00

  • CEP3 levels affect starvation-related growth responses of the primary root.

    abstract::CEPs (C-TERMINALLY ENCODED PEPTIDEs) inhibit Arabidopsis primary root growth by unknown mechanisms. We investigated how CEP3 levels control primary root growth. CEP3 peptide application decreased cell division, S-phase cell number, root meristematic cell number, and meristem zone (MZ) size in a dose- and CEP RECEPTOR1...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Delay C,Chapman K,Taleski M,Wang Y,Tyagi S,Xiong Y,Imin N,Djordjevic MA

    更新日期:2019-09-24 00:00:00

  • From museums to genomics: old herbarium specimens shed light on a C3 to C4 transition.

    abstract::Collections of specimens held by natural history museums are invaluable material for biodiversity inventory and evolutionary studies, with specimens accumulated over 300 years readily available for sampling. Unfortunately, most museum specimens yield low-quality DNA. Recent advances in sequencing technologies, so call...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Besnard G,Christin PA,Malé PJ,Lhuillier E,Lauzeral C,Coissac E,Vorontsova MS

    更新日期:2014-12-01 00:00:00

  • Integrating modelling and phenotyping approaches to identify and screen complex traits: transpiration efficiency in cereals.

    abstract::Following advances in genetics, genomics, and phenotyping, trait selection in breeding is limited by our ability to understand interactions within the plant and with the environment, and to identify traits of most relevance to the target population of environments. We propose an integrated approach that combines insig...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Chenu K,Van Oosterom EJ,McLean G,Deifel KS,Fletcher A,Geetika G,Tirfessa A,Mace ES,Jordan DR,Sulman R,Hammer GL

    更新日期:2018-06-06 00:00:00

  • Resistances along the CO2 diffusion pathway inside leaves.

    abstract::CO(2) faces a series of resistances while diffusing between the substomatal cavities and the sites of carboxylation within chloroplasts. The absence of techniques to measure the resistance of individual steps makes it difficult to define their relative importance. Resistance to diffusion through intercellular airspace...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章,评审


    authors: Evans JR,Kaldenhoff R,Genty B,Terashima I

    更新日期:2009-01-01 00:00:00

  • TOPOISOMERASE 6B is involved in chromatin remodelling associated with control of carbon partitioning into secondary metabolites and cell walls, and epidermal morphogenesis in Arabidopsis.

    abstract::Plant growth is continuous and modular, a combination that allows morphogenesis by cell division and elongation and serves to facilitate adaptation to changing environments. The pleiotropic phenotypes of the harlequin (hlq) mutant, isolated on the basis of ectopic expression of the abscisic acid (ABA)- and auxin-induc...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Mittal A,Balasubramanian R,Cao J,Singh P,Subramanian S,Hicks G,Nothnagel EA,Abidi N,Janda J,Galbraith DW,Rock CD

    更新日期:2014-08-01 00:00:00

  • Genetic dissection of the developmental behaviours of plant height in wheat under diverse water regimes.

    abstract::Plant height (PH), a crucial trait related to yield potential in crop plants, is known to be typically quantitatively inherited. However, its full expression can be inhibited by a limited water supply. In this study, the genetic basis of the developmental behaviour of PH was assessed in a 150-line wheat (Triticum aest...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Wu X,Wang Z,Chang X,Jing R

    更新日期:2010-06-01 00:00:00

  • Beyond the darkness: recent lessons from etiolation and de-etiolation studies.

    abstract::The state of etiolation is generally defined by the presence of non-green plastids (etioplasts) in plant tissues that would normally contain chloroplasts. In the commonly used dark-grown seedling system, etiolation is coupled with a type of growth called skotomorphogenesis. Upon illumination, de-etiolation occurs, mar...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Armarego-Marriott T,Sandoval-Ibañez O,Kowalewska Ł

    更新日期:2020-02-19 00:00:00

  • Revealing diversity in structural and biochemical forms of C4 photosynthesis and a C3-C4 intermediate in genus Portulaca L. (Portulacaceae).

    abstract::Portulacaceae is one of 19 families of terrestrial plants in which species having C(4) photosynthesis have been found. Representative species from major clades of the genus Portulaca were studied to characterize the forms of photosynthesis structurally and biochemically. The species P. amilis, P. grandiflora, P. molok...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Voznesenskaya EV,Koteyeva NK,Edwards GE,Ocampo G

    更新日期:2010-08-01 00:00:00

  • The evolution, function, structure, and expression of the plant sHSPs.

    abstract::Small heat shock proteins are a diverse, ancient, and important family of proteins. All organisms possess small heat shock proteins (sHSPs), indicating that these proteins evolved very early in the history of life prior to the divergence of the three domains of life (Archaea, Bacteria, and Eukarya). Comparing the stru...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章,评审


    authors: Waters ER

    更新日期:2013-01-01 00:00:00

  • Concerted changes in N and C primary metabolism in alfalfa (Medicago sativa) under water restriction.

    abstract::Although the mechanisms of nodule N(2) fixation in legumes are now well documented, some uncertainty remains on the metabolic consequences of water deficit. In most cases, little consideration is given to other organs and, therefore, the coordinated changes in metabolism in leaves, roots, and nodules are not well know...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Aranjuelo I,Tcherkez G,Molero G,Gilard F,Avice JC,Nogués S

    更新日期:2013-02-01 00:00:00

  • Combining QTL and co-expression analysis allowed identification of new candidates for oil accumulation in rapeseed.

    abstract::Quantitative trait loci (QTL) have been discovered in crops, where some of causal quantitative trait genes (QTGs) may not be functionally characterized even in model plant Arabidopsis. We propose an approach to delineate QTGs by coordinating expression of genes located within QTL in crops and known orthologs related w...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Cui Y,Zeng X,Xiong Q,Wei D,Liao J,Xu Y,Chen G,Zhou Y,Dong H,Wan H,Liu Z,Li J,Guo L,Jung C,He Y,Qian W

    更新日期:2020-11-29 00:00:00

  • Effect of in vitro cold acclimation of Deschampsia antarctica on the accumulation of proteins with antifreeze activity.

    abstract::Deschampsia antarctica has managed to colonize the maritime Antarctic. One of the main factors associated with its tolerance to low temperatures is the presence of apoplastic proteins with antifreeze activity. This work focuses on the effect of cold acclimation of D. antarctica on the accumulation of apoplastic protei...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Short S,Díaz R,Quiñones J,Beltrán J,Farías JG,Graether SP,Bravo LA

    更新日期:2020-05-30 00:00:00

  • Regulation of flooding tolerance of SAG12:ipt Arabidopsis plants by cytokinin.

    abstract::A SAG12:ipt gene construct, which increases cytokinin biosynthesis in response to senescence, was introduced into Arabidopsis plants to delay senescence induced by flooding stress. Two forms of flooding stress, including total submergence and root waterlogging, were applied to SAG12:ipt (IPT) and wild-type (WT) plants...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Huynh le N,Vantoai T,Streeter J,Banowetz G

    更新日期:2005-05-01 00:00:00

  • Metabolic routes affecting rubber biosynthesis in Hevea brasiliensis latex.

    abstract::The cytosolic mevalonate (MVA) pathway in Hevea brasiliensis latex is the conventionally accepted pathway which provides isopentenyl diphosphate (IPP) for cis-polyisoprene (rubber) biosynthesis. However, the plastidic 2-C-methyl-D-erythritol 4-phosphate (MEP) pathway may be an alternative source of IPP since its more ...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Chow KS,Mat-Isa MN,Bahari A,Ghazali AK,Alias H,Mohd-Zainuddin Z,Hoh CC,Wan KL

    更新日期:2012-03-01 00:00:00

  • β-Carboxysome bioinformatics: identification and evolution of new bacterial microcompartment protein gene classes and core locus constraints.

    abstract::Carboxysomes are bacterial microcompartments (BMCs) that enhance CO2 fixation in all cyanobacteria. Structurally, carboxysome shell proteins are classified according to the type of oligomer formed: hexameric (BMC-H), trimeric (BMC-T) and pentameric (BMC-P) proteins. To understand the forces driving the evolution of th...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Sommer M,Cai F,Melnicki M,Kerfeld CA

    更新日期:2017-06-01 00:00:00

  • Fatty acid elongation is important in the activity of thiocarbamate herbicides and in safening by dichlormid.

    abstract::The thiocarbamates, such as pebulate (S-propyl butyl (ethyl) thiocarbamate) are a well-established class of herbicides. They inhibit fatty acid elongation, which is necessary for the biosynthesis of constituents of surface waxes and suberin and this has been proposed to be important for their toxicity. In this study l...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Baldwin A,Rogers HJ,Francis D,Harwood JL

    更新日期:2003-04-01 00:00:00

  • Cloning and characterization of a phospholipase C from the C(4) plant Digitaria sanguinalis.

    abstract::As a PLC activity was implicated in the light transduction pathway that controls C(4) photosynthesis in Digitaria sanguinalis, a full length PLC cDNA (DsPLC2) was cloned. The proteins encoded by the two possible open reading frames were produced in Escherichia coli; they both harbour a PLC activity but with different ...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Coursol S,Pierre JN,Vidal J,Grisvard J

    更新日期:2002-06-01 00:00:00

  • Regulation of mitochondrial calcium in plants versus animals.

    abstract::Ca(2+) acts as an important cellular second messenger in eukaryotes. In both plants and animals, a wide variety of environmental and developmental stimuli trigger Ca(2+) transients of a specific signature that can modulate gene expression and metabolism. In animals, mitochondrial energy metabolism has long been consid...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章,评审


    authors: Wagner S,De Bortoli S,Schwarzländer M,Szabò I

    更新日期:2016-06-01 00:00:00

  • Expression of characteristics of ammonium nutrition as affected by pH of the root medium.

    abstract::To study the effect of root-zone pH on characteristic responses of NH4+ -fed plants, soybeans (Glycine max¿L.¿ Merr. cv. Ransom) were grown in flowing solution culture for 21 d on four sources of N (1.0 mol m-3 NO3-, 0.67 mol m-3 NO3- plus 0.33 mol m-3 NH4+, 0.33 mol m-3 NO3- plus 0.67 mol m-3 NH4+, and 1.0 mol m-3 NH...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Chaillou S,Vessey JK,Morot-Gaudry JF,Raper CD Jr,Henry LT,Boutin JP

    更新日期:1991-02-01 00:00:00

  • Transient response of sap flow to wind speed.

    abstract::Transient responses of sap flow to step changes in wind speed were experimentally investigated in a wind tunnel. A Granier-type sap flow sensor was calibrated and tested in a cylindrical tube for analysis of its transient time response. Then the sensor was used to measure the transient response of a well-watered Pachi...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Chu CR,Hsieh CI,Wu SY,Phillips NG

    更新日期:2009-01-01 00:00:00

  • Soil abiotic factors influence interactions between belowground herbivores and plant roots.

    abstract::Root herbivores are important ecosystem drivers and agricultural pests, and, possibly as a consequence, plants protect their roots using a variety of defensive strategies. One aspect that distinguishes belowground from aboveground plant-insect interactions is that roots are constantly exposed to a set of soil-specific...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章,评审


    authors: Erb M,Lu J

    更新日期:2013-03-01 00:00:00

  • Analysis of potential redundancy among Arabidopsis 6-phosphogluconolactonase isoforms in peroxisomes.

    abstract::Recent work revealed that PGD2, an Arabidopsis 6-phosphogluconate dehydrogenase (6-PGD) catalysing the third step of the oxidative pentose-phosphate pathway (OPPP) in peroxisomes, is essential during fertilization. Earlier studies on the second step, catalysed by PGL3, a dually targeted Arabidopsis 6-phosphogluconolac...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Lansing H,Doering L,Fischer K,Baune MC,Schaewen AV

    更新日期:2020-01-23 00:00:00

  • Xylem tension affects growth-induced water potential and daily elongation of maize leaves.

    abstract::Diurnal rates of leaf elongation vary in maize (Zea mays L.) and are characterized by a decline each afternoon. The cause of the afternoon decline was investigated. When the atmospheric environment was held constant in a controlled environment, and water and nutrients were adequately supplied to the soil or the roots ...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Tang AC,Boyer JS

    更新日期:2008-01-01 00:00:00

  • Ketocarotenoid formation in transgenic potato.

    abstract::Potato has been genetically engineered for the production of commercially important ketocarotenoids including astaxanthin (3,3'-dihydroxy 4,4'-diketo-beta-carotene). To support the formation of 3-hydroxylated and 4-ketolated beta-carotene, a transgenic potato line accumulating zeaxanthin due to inactivated zeaxanthin ...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Gerjets T,Sandmann G

    更新日期:2006-01-01 00:00:00