Foxtail mosaic virus-induced flowering assays in monocot crops.


:Virus-induced flowering (VIF) exploits RNA or DNA viruses to express flowering time genes to induce flowering in plants. Such plant virus-based tools have recently attracted widespread attention for their fundamental and applied uses in flowering physiology and in accelerating breeding in dicotyledonous crops and woody fruit-trees. We now extend this technology to a monocot grass and a cereal crop. Using a Foxtail mosaic virus (FoMV)-based VIF system, dubbed FoMViF, we showed that expression of florigenic Flowering Locus T (FT) genes can promote early flowering and spikelet development in proso millet, a C4 grass species with potential as a nutritional food and biofuel resource, and in non-vernalized C3 wheat, a major food crop worldwide. Floral and spikelet/grain induction in the two monocot plants was caused by the virally expressed untagged or FLAG-tagged FT orthologs, and the florigenic activity of rice Hd3a was more pronounced than its dicotyledonous counterparts in proso millet. The FoMViF system is easy to use and its efficacy to induce flowering and early spikelet/grain production is high. In addition to proso millet and wheat, we envisage that FoMViF will be also applicable to many economically important monocotyledonous food and biofuel crops.


J Exp Bot


Yuan C,Li H,Qin C,Zhang X,Chen Q,Zhang P,Xu X,He M,Zhang X,Tör M,Xue D,Wang H,Jackson S,He Y,Liu Y,Shi N,Hong Y




Has Abstract


2020-05-30 00:00:00














  • Brefeldin A affects adhesion of zoospores of the green alga Enteromorpha.

    abstract::Primary adhesion of zoospores of the green macroalga Enteromorpha to substrata involves a massive release of adhesive glycoproteins from Golgi-derived, membrane-bounded vesicles in the anterior region of the spore, followed by rapid curing. This process is sensitive to low concentrations (5-10 microg x ml(-1)) of the ...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Callow ME,Crawford S,Wetherbee R,Taylor K,Finlay JA,Callow JA

    更新日期:2001-07-01 00:00:00

  • Molecular mechanism for jasmonate-induction of anthocyanin accumulation in Arabidopsis.

    abstract::Anthocyanins are important plant pigments that fulfil many physiological and ecological functions. Anthocyanin biosynthesis is controlled by numerous regulatory factors at the transcriptional level. Jasmonates (JAs) has been shown to induce anthocyanin accumulation in several plant species, however, the molecular mech...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Shan X,Zhang Y,Peng W,Wang Z,Xie D

    更新日期:2009-01-01 00:00:00

  • Activation of ethylene-responsive p-hydroxyphenylpyruvate dioxygenase leads to increased tocopherol levels during ripening in mango.

    abstract::Mango is characterized by high tocopherol and carotenoid content during ripening. From a cDNA screen of differentially expressing genes during mango ripening, a full-length p-hydroxyphenylpyruvate dioxygenase (MiHPPD) gene homologue was isolated that encodes a key enzyme in the biosynthesis of tocopherols. The gene en...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Singh RK,Ali SA,Nath P,Sane VA

    更新日期:2011-06-01 00:00:00

  • To grow or not to grow: what can we learn on ethylene-gibberellin cross-talk by in silico gene expression analysis?

    abstract::Ethylene and gibberellins (GAs) are known to influence plant growth by mutual cross-talk and by interaction with other hormones. Transcript meta-analysis shows that GA and ethylene metabolism genes are expressed in the majority of plant organs. Both GAs and the ethylene precursor 1-amino-cyclopropane-1-carboxylic acid...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章,评审


    authors: Dugardeyn J,Vandenbussche F,Van Der Straeten D

    更新日期:2008-01-01 00:00:00

  • Alternative splicing of basic chitinase gene PR3b in the low-nicotine mutants of Nicotiana tabacum L. cv. Burley 21.

    abstract::Two unlinked semi-dominant loci, A (NIC1) and B (NIC2), control nicotine and related alkaloid biosynthesis in Burley tobaccos. Mutations in either or both loci (nic1 and nic2) lead to low nicotine phenotypes with altered environmental stress responses. Here we show that the transcripts derived from the pathogenesis-re...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Ma H,Wang F,Wang W,Yin G,Zhang D,Ding Y,Timko MP,Zhang H

    更新日期:2016-10-01 00:00:00

  • Mutation in xyloglucan 6-xylosytransferase results in abnormal root hair development in Oryza sativa.

    abstract::Root hairs are important for nutrient uptake, anchorage, and plant-microbe interactions. From a population of rice (Oryza sativa) mutagenized by ethyl methanesulfonate (EMS), a short root hair2 (srh2) mutant was identified. In hydroponic culture, srh2 seedlings were significantly reduced in root hair length. Bubble-li...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Wang C,Li S,Ng S,Zhang B,Zhou Y,Whelan J,Wu P,Shou H

    更新日期:2014-08-01 00:00:00

  • A disulfide bond A-like oxidoreductase is a strong candidate gene for self-incompatibility in apricot (Prunus armeniaca) pollen.

    abstract::S-RNase based gametophytic self-incompatibility (SI) is a widespread prezygotic reproductive barrier in flowering plants. In the Solanaceae, Plantaginaceae and Rosaceae gametophytic SI is controlled by the pistil-specific S-RNases and the pollen S-locus F-box proteins but non-S-specific factors, namely modifiers, are ...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Muñoz-Sanz JV,Zuriaga E,Badenes ML,Romero C

    更新日期:2017-11-02 00:00:00

  • Phosphatidylinositol-phospholipase C2 regulates pattern-triggered immunity in Nicotiana benthamiana.

    abstract::Phospholipid signaling plays an important role in plant immune responses against phytopathogenic bacteria in Nicotiana benthamiana. Here, we isolated two phospholipase C2 (PLC2) orthologs in the N. benthamiana genome, designated as PLC2-1 and 2-2. Both NbPLC2-1 and NbPLC2-2 were expressed in most tissues and were indu...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Kiba A,Nakano M,Hosokawa M,Galis I,Nakatani H,Shinya T,Ohnishi K,Hikichi Y

    更新日期:2020-08-06 00:00:00

  • Drought constraints on C4 photosynthesis: stomatal and metabolic limitations in C3 and C4 subspecies of Alloteropsis semialata.

    abstract::The C4 photosynthetic pathway uses water more efficiently than the C3 type, yet biogeographical analyses show a decline in C4 species relative to C3 species with decreasing rainfall. To investigate this paradox, the hypothesis that the C4 advantage over C3 photosynthesis is diminished by drought was tested, and the un...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Ripley BS,Gilbert ME,Ibrahim DG,Osborne CP

    更新日期:2007-01-01 00:00:00

  • A physical, enzymatic, and genetic characterization of perturbations in the seeds of the brownseed tomato mutants.

    abstract::The brownseed mutants (bs(1), bs(2), and bs(4)) of tomato all possess dark testae and deleteriously affect seed germination speed and/or final percentage. Poor germination performance of the bs(1) but not the bs(4) mutant, was due to greater impediment to radicle egress. Testa toughening (bs(1)) was prevented by dryin...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Downie AB,Dirk LM,Xu Q,Drake J,Zhang D,Dutt M,Butterfield A,Geneve RR,Corum JW 3rd,Lindstrom KG,Snyder JC

    更新日期:2004-05-01 00:00:00

  • Variation of floret fertility in hexaploid wheat revealed by tiller removal.

    abstract::Grain number per spike, which is greatly influenced by floret fertility, is an important trait of wheat (Triticum aestivum L.) yield. Maximum floret primordia, fertile floret, and final grain number per spikelet are three crucial factors of floret fertility. Floral degradation plays a critical role in determining thes...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Guo Z,Schnurbusch T

    更新日期:2015-09-01 00:00:00

  • Changes in fatty acid composition during development of tissues of coconut (Cocos nucifera L.) embryos in the intact nut and in vitro.

    abstract::Intact coconuts were germinated in situ and compared with excised zygotic embryos germinated in vitro. The growth of the embryonic tissue and their fatty acid compositions were measured. Haustoria, plumules and radicles of coconuts germinated in situ grew continuously and proportionately throughout the 120 d experimen...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: López-Villalobos A,Dodds PF,Hornung R

    更新日期:2001-05-01 00:00:00

  • Novel Insights into Host Receptors and Receptor-mediated Signaling that Regulate Arbuscular Mycorrhizal Symbiosis.

    abstract::More than 80% of land plant species benefit from symbiotic partnerships with arbuscular mycorrhizal (AM) fungi that assist in nutrient acquisition and enhance the ability of host plants to adapt to environmental constraints. Host-generated plasma membrane-residing receptor-like kinases and the α/β-hydrolases, e.g. DWA...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Nasir F,Bahadur A,Lin X,Gao Y,Tian C

    更新日期:2020-11-30 00:00:00

  • Calcium Signaling during Salt Stress and in the Regulation of Ion Homeostasis.

    abstract::Soil composition largely defines the living conditions of plants and represents one of their most relevant, dynamic and complex environmental cues. The effective concentrations of many either tolerated or essential ions and compounds in the soil usually differ from the optimum that would be most suitable for plants. I...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Manishankar P,Wang N,Köster P,Alatar AA,Kudla J

    更新日期:2018-05-24 00:00:00

  • Molecular and functional analysis of a brown planthopper resistance protein with two nucleotide binding site domains.

    abstract::The brown planthopper (Nilaparvata lugens Stål, BPH) resistance gene BPH9 encodes an unusual coiled-coil (CC) nucleotide-binding leucine-rich repeat (LRR) protein with two NBS (Nuceotide binding site) domains. To understand how this CC-NBS-NBS-LRR protein regulates defense signaling and BPH resistance, we dissected ea...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Wang Z,Huang J,Nie L,Hu Y,Zhang N,Guo Q,Guo J,Du B,Zhu L,He G,Chen R

    更新日期:2020-12-21 00:00:00

  • Rutin in buckwheat herbs grown at different UV-B radiation levels: comparison of two UV spectrophotometric and an HPLC method.

    abstract::Rutin is an antioxidant with many interesting pharmacological effects. It can also be found in buckwheat (Fagopyrum esculentum Moench). UV radiation stimulates the activity of enzymes of the phenylpropanoid pathway and there is some evidence that it influences the rutin content in plants. The aim of the present resear...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Kreft S,Strukelj B,Gaberscik A,Kreft I

    更新日期:2002-08-01 00:00:00

  • Transporters involved in mineral nutrient uptake in rice.

    abstract::One of the most important roles of plant roots is to take up essential mineral nutrients from the soil for use in plant growth and development. The uptake of mineral elements is mediated by various transporters belonging to different transporter families. Here we reviewed transporters for the uptake of macronutrients ...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章,评审


    authors: Sasaki A,Yamaji N,Ma JF

    更新日期:2016-06-01 00:00:00

  • Fine mapping of a quantitative trait locus for spikelet number per panicle in a new plant type rice and evaluation of a near-isogenic line for grain productivity.

    abstract::Total spikelet number per panicle (TSN) is one of the determinants of grain productivity in rice (Oryza sativa L.). In this study, we attempted to detect quantitative trait loci (QTLs) for TSN in the introgression lines with high TSN, derived from the cross of Indica Group variety IR 64 with new plant type lines. Two ...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Sasaki K,Fujita D,Koide Y,Lumanglas PD,Gannaban RB,Tagle AG,Obara M,Fukuta Y,Kobayashi N,Ishimaru T

    更新日期:2017-05-17 00:00:00

  • Changes in the epigenome and transcriptome of the poplar shoot apical meristem in response to water availability affect preferentially hormone pathways.

    abstract::The adaptive capacity of long-lived organisms such as trees to the predicted climate changes, including severe and successive drought episodes, will depend on the presence of genetic diversity and phenotypic plasticity. Here, the involvement of epigenetic mechanisms in phenotypic plasticity toward soil water availabil...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Lafon-Placette C,Le Gac AL,Chauveau D,Segura V,Delaunay A,Lesage-Descauses MC,Hummel I,Cohen D,Jesson B,Le Thiec D,Bogeat-Triboulot MB,Brignolas F,Maury S

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  • Barley plants over-expressing the NAC transcription factor gene HvNAC005 show stunting and delay in development combined with early senescence.

    abstract::The plant-specific NAC transcription factors have attracted particular attention because of their involvement in stress responses, senescence, and nutrient remobilization. The HvNAC005 gene of barley encodes a protein belonging to subgroup NAC-a6 of the NAC family. This study shows that HvNAC005 is associated with dev...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Christiansen MW,Matthewman C,Podzimska-Sroka D,O'Shea C,Lindemose S,Møllegaard NE,Holme IB,Hebelstrup K,Skriver K,Gregersen PL

    更新日期:2016-09-01 00:00:00

  • Sustained enhancement of photosynthesis in coffee trees grown under free-air CO2 enrichment conditions: disentangling the contributions of stomatal, mesophyll, and biochemical limitations.

    abstract::Coffee (Coffea spp.), a globally traded commodity, is a slow-growing tropical tree species that displays an improved photosynthetic performance when grown under elevated atmospheric CO2 concentrations ([CO2]). To investigate the mechanisms underlying this response, two commercial coffee cultivars (Catuaí and Obatã) we...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: DaMatta FM,Godoy AG,Menezes-Silva PE,Martins SC,Sanglard LM,Morais LE,Torre-Neto A,Ghini R

    更新日期:2016-01-01 00:00:00

  • Translational researches on leaf senescence for enhancing plant productivity and quality.

    abstract::Leaf senescence is a very important trait that limits yield and biomass accumulation of agronomic crops and reduces post-harvest performance and the nutritional value of horticultural crops. Significant advance in physiological and molecular understanding of leaf senescence has made it possible to devise ways of manip...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章,评审


    authors: Guo Y,Gan SS

    更新日期:2014-07-01 00:00:00

  • RuBisCO-like proteins as the enolase enzyme in the methionine salvage pathway: functional and evolutionary relationships between RuBisCO-like proteins and photosynthetic RuBisCO.

    abstract::Ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) is the key enzyme in the fixation of CO(2) in the Calvin cycle of plants. Many genome projects have revealed that bacteria, including Bacillus subtilis, possess genes for proteins that are similar to the large subunit of RuBisCO. These RuBisCO homologues are ca...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Ashida H,Saito Y,Nakano T,Tandeau de Marsac N,Sekowska A,Danchin A,Yokota A

    更新日期:2008-01-01 00:00:00

  • Organization of pectic arabinan and galactan side chains in association with cellulose microfibrils in primary cell walls and related models envisaged.

    abstract::The structure of arabinan and galactan domains in association with cellulose microfibrils was investigated using enzymatic and alkali degradation procedures. Sugar beet and potato cell wall residues (called 'natural' composites), rich in pectic neutral sugar side chains and cellulose, as well as 'artificial' composite...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Zykwinska A,Thibault JF,Ralet MC

    更新日期:2007-01-01 00:00:00

  • Nitrite and nitric oxide are important in the adjustment of primary metabolism during the hypersensitive response in tobacco.

    abstract::Nitrate and ammonia deferentially modulate primary metabolism during the hypersensitive response in tobacco. In this study, tobacco RNAi lines with low nitrite reductase (NiRr) levels were used to investigate the roles of nitrite and nitric oxide (NO) in this process. The lines accumulate NO2-, with increased NO gener...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Mur LAJ,Kumari A,Brotman Y,Zeier J,Mandon J,Cristescu SM,Harren F,Kaiser WM,Fernie AR,Gupta KJ

    更新日期:2019-08-29 00:00:00

  • Function of pea amino acid permease AAP6 in nodule nitrogen metabolism and export, and plant nutrition.

    abstract::Legumes fix atmospheric nitrogen through a symbiotic relationship with bacteroids in root nodules. Following fixation in pea (Pisum sativum L.) nodules, nitrogen is reduced to amino acids that are exported via the nodule xylem to the shoot, and in the phloem to roots in support of growth. However, the mechanisms invol...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Garneau MG,Tan Q,Tegeder M

    更新日期:2018-10-12 00:00:00

  • The multi-protein family of Arabidopsis sulphotransferases and their relatives in other plant species.

    abstract::All members of the sulphotransferase (SOT, EC 2.8.2.-) protein family use 3'-phosphoadenosine 5'-phosphosulphate (PAPS) as the sulphuryl donor and transfer the sulphonate group to an appropriate hydroxyl group of several classes of substrates. These enzymes have highly conserved domains and can be found in eubacteria ...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章,评审


    authors: Klein M,Papenbrock J

    更新日期:2004-08-01 00:00:00

  • Characterization of expression, and cloning, of beta-D-xylosidase and alpha-L-arabinofuranosidase in developing and ripening tomato (Lycopersicon esculentum Mill.) fruit.

    abstract::Modifications to the cell wall of developing and ripening tomato fruit are mediated by cell wall-degrading enzymes, including a beta-d-xylosidase or alpha-l-arabinofuranosidase, which participate in the breakdown of xylans and/or arabinoxylans. The activity of both enzymes was highest during early fruit growth, before...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Itai A,Ishihara K,Bewley JD

    更新日期:2003-12-01 00:00:00

  • 'Movers and shakers' in the regulation of fruit ripening: a cross-dissection of climacteric versus non-climacteric fruit.

    abstract::Fruit ripening is a complex and highly coordinated developmental process involving the expression of many ripening-related genes under the control of a network of signalling pathways. The hormonal control of climacteric fruit ripening, especially ethylene perception and signalling transduction in tomato has been well ...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章,评审


    authors: Cherian S,Figueroa CR,Nair H

    更新日期:2014-09-01 00:00:00

  • On the mechanism of C4 photosynthesis intermediate exchange between Kranz mesophyll and bundle sheath cells in grasses.

    abstract::C(4) photosynthesis involves cell-to-cell exchange of photosynthetic intermediates between the Kranz mesophyll (KMS) and bundle sheath (BS) cells. This was believed to occur by simple diffusion through plentiful plasmodesmatal (PD) connections between these cell types. The model of C(4) intermediates' transport was el...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Sowiński P,Szczepanik J,Minchin PE

    更新日期:2008-01-01 00:00:00