Abstract：
:Transient kinetics of net H+, K+, Ca2+, and Cl- fluxes were measured non-invasively, using an ion-selective microelectrode technique, for bean (Vicia faba L.) leaf mesophyll in response to 150 mM mannitol treatment. In a parallel set of experiments, changes in the plasma membrane potential and the total proline content in leaves were monitored. Regardless of the ionic composition of the bath solution, hyperosmotic stress caused a significant increase in the K+ and Cl- uptake into mesophyll cells. At the same time, no significant proline changes were observed for at least 16 h after the onset of stress. Experiments with inhibitors suggested that potassium inward rectifier (KIR) channels, exhibiting mechanosensitive properties and acting as primary receptors of osmotic stress, are likely to be involved. Due to the coupling by membrane potential, changes in K+ and Cl- transport may modify activity of the plasma membrane H+-pump. Such coupling may also be responsible for the mannitol-induced oscillations (period of about 4 min) in net ion fluxes observed in 90% of plants. Calculations show that influx of K+ and Cl- observed in response to hyperosmotic treatment may provide an adequate osmotic adjustment in bean mesophyll, which suggests that the activity of the plasma membrane transporters for these ions should be targeted to improve osmotolerance, at least in this crop.
journal_name
J Exp Botjournal_title
Journal of experimental botanyauthors
Shabala S,Babourina O,Newman Ikeywords:
subject
Has Abstractpub_date
2000-07-01 00:00:00pages
1243-53issue
348eissn
0022-0957issn
1460-2431journal_volume
51pub_type
杂志文章abstract：:The reproductive success of plants largely depends on the correct programming of developmental phase transitions, particularly the shift from vegetative to reproductive growth. The timing of this transition is finely regulated by the integration of an array of environmental and endogenous factors. Nitrogen is the mine...
journal_title：Journal of experimental botany
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journal_title：Journal of experimental botany
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更新日期：2005-10-01 00:00:00
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pub_type： 杂志文章
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journal_title：Journal of experimental botany
pub_type： 杂志文章
doi：10.1093/jexbot/51.344.645
更新日期：2000-03-01 00:00:00
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journal_title：Journal of experimental botany
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更新日期：2009-01-01 00:00:00
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更新日期：2008-01-01 00:00:00
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更新日期：2008-01-01 00:00:00
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