Subcellular localization and interactions among rubber particle proteins from Hevea brasiliensis.

Abstract:

:Natural rubber (polyisoprene) from the rubber tree Hevea brasiliensis is synthesized by specialized cells called laticifers. It is not clear how rubber particles arise, although one hypothesis is that they derive from the endoplasmic reticulum (ER) membrane. Here we cloned the genes encoding four key proteins found in association with rubber particles and studied their intracellular localization by transient expression in Nicotiana benthamiana leaves. We show that, while the cis-prenyltransferase (CPT), responsible for the synthesis of long polyisoprene chains, is a soluble, cytosolic protein, other rubber particle proteins such as rubber elongation factor (REF), small rubber particle protein (SRPP) and Hevea rubber transferase 1-REF bridging protein (HRBP) are associated with the endoplasmic reticulum (ER). We also show that SRPP can recruit CPT to the ER and that interaction of CPT with HRBP leads to both proteins relocating to the plasma membrane. We discuss these results in the context of the biogenesis of rubber particles.

journal_name

J Exp Bot

authors

Brown D,Feeney M,Ahmadi M,Lonoce C,Sajari R,Di Cola A,Frigerio L

doi

10.1093/jxb/erx331

subject

Has Abstract

pub_date

2017-11-02 00:00:00

pages

5045-5055

issue

18

eissn

0022-0957

issn

1460-2431

pii

4211097

journal_volume

68

pub_type

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