Abstract:
:Natural rubber (polyisoprene) from the rubber tree Hevea brasiliensis is synthesized by specialized cells called laticifers. It is not clear how rubber particles arise, although one hypothesis is that they derive from the endoplasmic reticulum (ER) membrane. Here we cloned the genes encoding four key proteins found in association with rubber particles and studied their intracellular localization by transient expression in Nicotiana benthamiana leaves. We show that, while the cis-prenyltransferase (CPT), responsible for the synthesis of long polyisoprene chains, is a soluble, cytosolic protein, other rubber particle proteins such as rubber elongation factor (REF), small rubber particle protein (SRPP) and Hevea rubber transferase 1-REF bridging protein (HRBP) are associated with the endoplasmic reticulum (ER). We also show that SRPP can recruit CPT to the ER and that interaction of CPT with HRBP leads to both proteins relocating to the plasma membrane. We discuss these results in the context of the biogenesis of rubber particles.
journal_name
J Exp Botjournal_title
Journal of experimental botanyauthors
Brown D,Feeney M,Ahmadi M,Lonoce C,Sajari R,Di Cola A,Frigerio Ldoi
10.1093/jxb/erx331subject
Has Abstractpub_date
2017-11-02 00:00:00pages
5045-5055issue
18eissn
0022-0957issn
1460-2431pii
4211097journal_volume
68pub_type
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