Pyramiding Mn-superoxide dismutase transgenes to improve persistence and biomass production in alfalfa.


:Expression of individual superoxide dismutase (SOD) transgenes improves environmental stress tolerance and biomass production in alfalfa (Medicago sativa L.). The objective of this study was to test the hypothesis that synergy exists between transgenic SOD stress-tolerance mechanisms, specifically that the simultaneous expression of two SOD transgenes confers greater benefit than the expression of a single SOD transgene. The hypothesis was tested through an evaluation of an F(1) family generated through a sexual cross of a hemizygous Mit-MnSOD plant and a hemizygous Chl-MnSOD-transgenic alfalfa plant which had previously been screened in field trials for improved persistence. Southern analyses revealed that the parents each had single insertion regions of the MnSOD cDNA and the inheritance followed the expected Mendelian ratios. Native PAGE gels and enzyme inhibition assays revealed the activity of the transgenic MnSOD isozymes. F(1) progeny containing either the Mit-MnSOD or the Chl-MnSOD transgene had significantly higher storage organ (crown+root) biomass compared to non-transgenic siblings. The joint expression of the transgenes resulted in a numerical increase in total SOD activity. However, F(1) progeny containing both transgenes had lower shoot and storage organ biomass compared to siblings having only one or the other transgene, a result that did not support the authors' hypothesis. It was postulated that a promoter with lower expression than 35S may be necessary if closely related transgenes are to be pyramided in the same plant.


J Exp Bot


Samis K,Bowley S,McKersie B



Has Abstract


2002-05-01 00:00:00












  • The E3 ligase ABI3-INTERACTING PROTEIN2 negatively regulates FUSCA3 and plays a role in cotyledon development in Arabidopsis thaliana.

    abstract::FUSCA3 (FUS3) is a short-lived B3-domain transcription factor that regulates seed development and phase transitions in Arabidopsis thaliana. The mechanisms controlling FUS3 levels are currently poorly understood. Here we show that FUS3 interacts with the RING E3 ligase ABI3-INTERACTING PROTEIN2 (AIP2). AIP2-green fluo...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Duong S,Vonapartis E,Li CY,Patel S,Gazzarrini S

    更新日期:2017-03-01 00:00:00

  • Class I KNOX genes are associated with organogenesis during bulbil formation in Agave tequilana.

    abstract::Bulbil formation in Agave tequilana was analysed with the objective of understanding this phenomenon at the molecular and cellular levels. Bulbils formed 14-45 d after induction and were associated with rearrangements in tissue structure and accelerated cell multiplication. Changes at the cellular level during bulbil ...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Abraham-Juárez MJ,Martínez-Hernández A,Leyva-González MA,Herrera-Estrella L,Simpson J

    更新日期:2010-09-01 00:00:00

  • Silicon decreases both uptake and root-to-shoot translocation of manganese in rice.

    abstract::Silicon (Si) is known to alleviate manganese (Mn) toxicity in a number of plant species; however, the mechanisms responsible for this effect are poorly understood. Here, we investigated the interaction between Si and Mn in rice (Oryza sativa) by using a mutant defective in Si uptake. Silicon alleviated Mn toxicity in ...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Che J,Yamaji N,Shao JF,Ma JF,Shen RF

    更新日期:2016-03-01 00:00:00

  • The ins and outs of CO2.

    abstract::It is difficult to distinguish influx and efflux of inorganic C in photosynthesizing tissues; this article examines what is known and where there are gaps in knowledge. Irreversible decarboxylases produce CO2, and CO2 is the substrate/product of enzymes that act as carboxylases and decarboxylases. Some irreversible ca...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Raven JA,Beardall J

    更新日期:2016-01-01 00:00:00

  • Gene expression profiling and silencing reveal that monolignol biosynthesis plays a critical role in penetration defence in wheat against powdery mildew invasion.

    abstract::Cell wall apposition (CWA) formation is one of the first lines of defence used by plants to halt invading fungi such as powdery mildew. Lignin is a complex polymer of hydroxylated and methoxylated phenylpropane units (monolignols) and lignification renders the cell wall more resistant to pathogen attack. The role of m...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Bhuiyan NH,Selvaraj G,Wei Y,King J

    更新日期:2009-01-01 00:00:00

  • Cross-talk between reactive oxygen species and polyamines in regulation of ion transport across the plasma membrane: implications for plant adaptive responses.

    abstract::Many stresses are associated with increased accumulation of reactive oxygen species (ROS) and polyamines (PAs). PAs act as ROS scavengers, but export of putrescine and/or PAs to the apoplast and their catabolization by amine oxidases gives rise to H2O2 and other ROS, including hydroxyl radicals ((•)OH). PA catabolizat...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章,评审


    authors: Pottosin I,Velarde-Buendía AM,Bose J,Zepeda-Jazo I,Shabala S,Dobrovinskaya O

    更新日期:2014-03-01 00:00:00

  • Utricularia carnivory revisited: plants supply photosynthetic carbon to traps.

    abstract::The rootless, aquatic Utricularia species belong to the largest and most cosmopolitan carnivorous plant genus. Populations of Utricularia plants are an important component of many standing, nutrient-poor, and humic waters. Carbon (C) allocation is an aspect of Utricularia's ecophysiology that has not been studied prev...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Sirová D,Borovec J,Santrůcková H,Santrucek J,Vrba J,Adamec L

    更新日期:2010-01-01 00:00:00

  • The peach (Prunus persica L. Batsch) genome harbours 10 KNOX genes, which are differentially expressed in stem development, and the class 1 KNOPE1 regulates elongation and lignification during primary growth.

    abstract::The KNOTTED-like (KNOX) genes encode homeodomain transcription factors and regulate several processes of plant organ development. The peach (Prunus persica L. Batsch) genome was found to contain 10 KNOX members (KNOPE genes); six of them were experimentally located on the Prunus reference map and the class 1 KNOPE1 wa...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Testone G,Condello E,Verde I,Nicolodi C,Caboni E,Dettori MT,Vendramin E,Bruno L,Bitonti MB,Mele G,Giannino D

    更新日期:2012-09-01 00:00:00

  • Functional roles of melatonin in plants, and perspectives in nutritional and agricultural science.

    abstract::The presence of melatonin in plants is universal. Evidence has confirmed that a major portion of the melatonin is synthesized by plants themselves even though a homologue of the classic arylalkylamine N-acetyltransferase (AANAT) has not been identified as yet in plants. Thus, the serotonin N-acetylating enzyme in plan...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章,评审


    authors: Tan DX,Hardeland R,Manchester LC,Korkmaz A,Ma S,Rosales-Corral S,Reiter RJ

    更新日期:2012-01-01 00:00:00

  • Light quality modulates metabolic synchronization over the diel phases of crassulacean acid metabolism.

    abstract::Temporal compartmentation of carboxylation processes is a defining feature of crassulacean acid metabolism and involves circadian control of key metabolic and transport steps that regulate the supply and demand for carbon over a 24h cycle. Recent insights on the molecular workings of the circadian clock and its connec...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Ceusters J,Borland AM,Taybi T,Frans M,Godts C,De Proft MP

    更新日期:2014-07-01 00:00:00

  • Ascorbic acid accumulates as a defense response to Turnip mosaic virus in resistant Brassica rapa cultivars.

    abstract::We initially observed that Brassica rapa cultivars containing the Turnip mosaic virus (TuMV) resistance gene, Rnt1-1, accumulated a high level of endogenous ascorbic acid (AS) and dehydroascobic acid (DHA) when infected with TuMV. We here hypothesized a possible contribution of an elevated level of AS+DHA (TAA) to the...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Fujiwara A,Togawa S,Hikawa T,Matsuura H,Masuta C,Inukai T

    更新日期:2016-07-01 00:00:00

  • Auxin-induced expression divergence between Arabidopsis species may originate within the TIR1/AFB-AUX/IAA-ARF module.

    abstract::Auxin is an essential regulator of plant growth and development, and auxin signaling components are conserved among land plants. Yet, a remarkable degree of natural variation in physiological and transcriptional auxin responses has been described among Arabidopsis thaliana accessions. As intraspecies comparisons offer...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Trenner J,Poeschl Y,Grau J,Gogol-Döring A,Quint M,Delker C

    更新日期:2017-01-01 00:00:00

  • Small RNA activity and function in angiosperm gametophytes.

    abstract::Small non-coding RNAs are key post-transcriptional and transcriptional regulators of plant gene expression in angiosperm sporophytes. In recent years, gametophytic small RNAs have also been investigated, predominantly in Arabidopsis male gametophytes, revealing features in common with the sporophyte as well as some su...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章,评审


    authors: Le Trionnaire G,Grant-Downton RT,Kourmpetli S,Dickinson HG,Twell D

    更新日期:2011-03-01 00:00:00

  • Foxtail mosaic virus-induced flowering assays in monocot crops.

    abstract::Virus-induced flowering (VIF) exploits RNA or DNA viruses to express flowering time genes to induce flowering in plants. Such plant virus-based tools have recently attracted widespread attention for their fundamental and applied uses in flowering physiology and in accelerating breeding in dicotyledonous crops and wood...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Yuan C,Li H,Qin C,Zhang X,Chen Q,Zhang P,Xu X,He M,Zhang X,Tör M,Xue D,Wang H,Jackson S,He Y,Liu Y,Shi N,Hong Y

    更新日期:2020-05-30 00:00:00

  • Identification of drought responsive proteins and related proteomic QTLs in barley.

    abstract::Drought is a major abiotic stress that negatively influences crop yield. Breeding strategies for improved drought resistance require an improved knowledge of plant drought responses. We therefore applied drought to barley recombinant inbred lines and their parental genotypes shortly before tillering. A large-scale pro...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Rodziewicz P,Chmielewska K,Sawikowska A,Marczak Ł,Łuczak M,Bednarek P,Mikołajczak K,Ogrodowicz P,Kuczyńska A,Krajewski P,Stobiecki M

    更新日期:2019-05-09 00:00:00

  • Phloem transport: a review of mechanisms and controls.

    abstract::It is generally believed that an osmotically generated pressure gradient drives the phloem mass flow. So far, this widely accepted Münch theory has required remarkably few adaptations, but the debate on alternative and additional hypotheses is still ongoing. Recently, a possible shortcoming of the Münch theory has bee...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章,评审


    authors: De Schepper V,De Swaef T,Bauweraerts I,Steppe K

    更新日期:2013-11-01 00:00:00

  • Effects of simultaneous expression of heterologous genes involved in phytochelatin biosynthesis on thiol content and cadmium accumulation in tobacco plants.

    abstract::Transgenic tobacco (Nicotiana tabacum cv. LA Burley 21) lines expressing three genes encoding enzymes thought to be critical for the efficient production of phytochelatins, (i) serine acetyltransferase (EC involved in the production of O-acetylserine, the cysteine precursor, (ii) gamma-glutamylcysteine synth...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Wawrzynski A,Kopera E,Wawrzynska A,Kaminska J,Bal W,Sirko A

    更新日期:2006-01-01 00:00:00

  • Long-term in vitro culture of grape berries and its application to assess the effects of sugar supply on anthocyanin accumulation.

    abstract::Grape berry development and ripening are under complex regulation by the nutrients, hormones, and environment cues sensed by the berry. However, the biochemical and molecular mechanisms underlying these types of regulation are poorly understood. A simplified but realistic model system that enables fruit growth conditi...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Dai ZW,Meddar M,Renaud C,Merlin I,Hilbert G,Delrot S,Gomès E

    更新日期:2014-08-01 00:00:00

  • Thermoperiodic growth control by gibberellin does not involve changes in photosynthetic or respiratory capacities in pea.

    abstract::Active gibberellin (GA(1)) is an important mediator of thermoperiodic growth in pea. Plants grown under lower day than night temperature (negative DIF) elongate less and have reduced levels of GA(1) compared with plants grown at higher day than night temperature (positive DIF). By comparing the wild type (WT) and the ...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Stavang JA,Pettersen RI,Wendell M,Solhaug KA,Junttila O,Moe R,Olsen JE

    更新日期:2010-02-01 00:00:00

  • The logic of communication: roles for mobile transcription factors in plants.

    abstract::Mobile transcription factors play many roles in plant development. Here, we compare the use of mobile transcription factors as signals with some canonical signal transduction processes in prokaryotes and eukaryotes. After an initial survey, we focus on the SHORT-ROOT pathway in Arabidopsis roots to show that, despite ...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章,评审


    authors: Long Y,Scheres B,Blilou I

    更新日期:2015-02-01 00:00:00

  • The uncoupling of respiration in plant mitochondria: Keeping reactive oxygen and nitrogen species under control.

    abstract::Plant mitochondrial respiration involves operation of various alternate pathways. These pathways participate, both directly and indirectly, in the maintenance of mitochondrial functions though they do not contribute to energy production being uncoupled from the generation of an electrochemical gradient across the mito...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Popov VN,Syromyatnikov MY,Fernie AR,Chakraborty S,Gupta KJ,Igamberdiev AU

    更新日期:2020-11-27 00:00:00

  • Diel patterns of leaf and root growth: endogenous rhythmicity or environmental response?

    abstract::Plants are sessile organisms forced to adjust to their surrounding environment. In a single plant the photoautotrophic shoot is exposed to pronounced environmental variations recurring in a day-night 24 h (diel) cycle, whereas the heterotrophic root grows in a temporally less fluctuating environment. The contrasting h...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章,评审


    authors: Ruts T,Matsubara S,Wiese-Klinkenberg A,Walter A

    更新日期:2012-05-01 00:00:00

  • Auxin regulation of Arabidopsis flower development involves members of the AINTEGUMENTA-LIKE/PLETHORA (AIL/PLT) family.

    abstract::Auxin is an important regulator of many aspects of plant growth and development. During reproductive development, auxin specifies the site of flower initiation and subsequently regulates organ growth and patterning as well as later events that determine reproductive success. Underlying auxin action in plant tissues is...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章,评审


    authors: Krizek BA

    更新日期:2011-06-01 00:00:00

  • Transcriptional reprogramming and enhanced photosynthesis drive inducible salt tolerance in sugarcane mutant line M4209.

    abstract::Sugarcane (Saccharum officinarum) is a globally cultivated cash crop whose yield is negatively affected by soil salinity. In this study, we investigated the molecular basis of inducible salt tolerance in M4209, a sugarcane mutant line generated through radiation-induced mutagenesis. Under salt-contaminated field condi...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Negi P,Pandey M,Dorn KM,Nikam AA,Devarumath RM,Srivastava AK,Suprasanna P

    更新日期:2020-10-07 00:00:00

  • Early cytokinin response proteins and phosphoproteins of Arabidopsis thaliana identified by proteome and phosphoproteome profiling.

    abstract::Cytokinins are plant hormones involved in regulation of diverse developmental and physiological processes in plants whose molecular mechanisms of action are being intensely researched. However, most rapid responses to cytokinin signals at the proteomic and phosphoproteomic levels are unknown. Early cytokinin responses...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Cerny M,Dycka F,Bobál'ová J,Brzobohaty B

    更新日期:2011-01-01 00:00:00

  • Greenhouse and field testing of transgenic wheat plants stably expressing genes for thaumatin-like protein, chitinase and glucanase against Fusarium graminearum.

    abstract::Genes encoding pathogenesis-related (PR-) proteins isolated from a cDNA library of Fusarium graminearum-infected wheat spikes of scab-resistant cultivar 'Sumai-3' were transformed into susceptible spring wheat, 'Bobwhite' using a biolistic transformation protocol, with the goal of enhancing levels of resistance agains...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Anand A,Zhou T,Trick HN,Gill BS,Bockus WW,Muthukrishnan S

    更新日期:2003-03-01 00:00:00

  • Variation in the k(cat) of Rubisco in C(3) and C(4) plants and some implications for photosynthetic performance at high and low temperature.

    abstract::The capacity of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) to consume RuBP is a major limitation on the rate of net CO(2) assimilation (A) in C(3) and C(4) plants. The pattern of Rubisco limitation differs between the two photosynthetic types, as shown by comparisons of temperature and CO(2) responses o...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Sage RF

    更新日期:2002-04-01 00:00:00

  • Differential responses of grapevine rootstocks to water stress are associated with adjustments in fine root hydraulic physiology and suberization.

    abstract::Water deficits are known to alter fine root structure and function, but little is known about how these responses contribute to differences in drought resistance across grapevine rootstocks. The ways in which water deficit affects root anatomical and physiological characteristics were studied in two grapevine rootstoc...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Barrios-Masias FH,Knipfer T,McElrone AJ

    更新日期:2015-09-01 00:00:00

  • Functional relationship between mTERF4 and GUN1 in retrograde signaling.

    abstract::Plastid-to-nucleus retrograde signaling plays an important role in regulating the expression of photosynthesis-associated nuclear genes (PhANGs) in accordance with physiological demands on chloroplast biogenesis and function. Despite its fundamental importance, little is known about the molecular nature of the plastid...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Sun X,Xu D,Liu Z,Kleine T,Leister D

    更新日期:2016-06-01 00:00:00

  • Regulation of high-affinity sulphate transporters in plants: towards systematic analysis of sulphur signalling and regulation.

    abstract::Plants require the function of plasma membrane-bound sulphate transporters for the initial uptake of inorganic sulphate. Part of this fundamental process is the energy-dependent proton/sulphate co-transport systems that are located in the surface cell layers of roots. During sulphur limitation, plants are able to acti...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章,评审


    authors: Maruyama-Nakashita A,Nakamura Y,Yamaya T,Takahashi H

    更新日期:2004-08-01 00:00:00