Abstract:
:Hydroponically grown spinach plants were deprived of an external source of sulphate after an initial period when the S-supply was sufficient. The time-course of events following this treatment was monitored. The first responses were found in the uptake and translocation of NO(3)(-) and the uptake of SO(4)(2-). The former declined by approximately 50%, the effect being most significant at higher [NO(3)(-)](ext.) while the latter increased 6-fold over a 4 d period. Growth in the absence of external SO(4)(2-) resulted in exhaustion of internal SO(4)(2-) pools, the effect being seen first in roots, then in young leaves and, after a marked delay, in mature leaves. In young leaves, there were dramatic increases in the [NO(3)(-)] and the content of arginine in the first 2 d of S-deprivation. The concentration of glutamine, the most abundant amino acid in S-sufficient conditions, also more than doubled in S-deficient young leaves. The changes in arginine levels were also found in older leaves, but the change in glutamine level was not seen. Assays of nitrate reductase activity (NRA) and nitrate reductase (NR) mRNA from young leaves of S-replete and S-deprived plants revealed a divergence in activity and content only late in the experiments (between days 4 and 8) when results were expressed on a unit leaf basis. However, there were also time-dependent changes in the protein content that kept the specific activities (NRA:protein and RNA:protein) more or less unchanged. The results imply that the impact of S-deficiency on N-utilization are more sensitively monitored by simple measurements of the chemical composition of young leaves than by measurements of NRA or NR transcript abundance. They also suggest that protein synthesis in young leaves is strongly dependent on a continuous supply of SO(4)(2-) from outside the plant.
journal_name
J Exp Botjournal_title
Journal of experimental botanyauthors
Prosser IM,Purves JV,Saker LR,Clarkson DTkeywords:
subject
Has Abstractpub_date
2001-01-01 00:00:00pages
113-21issue
354eissn
0022-0957issn
1460-2431journal_volume
52pub_type
杂志文章abstract::The phloem is a well-known target of sucking and piercing insects that utilize the transported fluid as their major nutrient source. In addition to small molecules like sugars and amino acids, phloem sap of higher land plants contains proteins that can accumulate up to high concentrations. Although the knowledge about...
journal_title:Journal of experimental botany
pub_type: 杂志文章,评审
doi:10.1093/jxb/erj087
更新日期:2006-01-01 00:00:00
abstract::Plants co-evolved with an enormous variety of microbial pathogens and insect herbivores under daily and seasonal variations in abiotic environmental conditions. Hence, plant cells display a high capacity to respond to diverse stresses through a flexible and finely balanced response network that involves components suc...
journal_title:Journal of experimental botany
pub_type: 杂志文章,评审
doi:10.1093/jxb/erw079
更新日期:2016-03-01 00:00:00
abstract::Sweet cherry is a non-climacteric fruit and its ripening is regulated by abscisic acid (ABA) during fruit development. In this study, four cDNAs (PacCYP707A1-4) encoding 8'-hydroxylase, a key enzyme in the oxidative catabolism of ABA, were identified in sweet cherry fruits using tobacco rattle virus-induced gene silen...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erv169
更新日期:2015-07-01 00:00:00
abstract::Previous studies documented that metal hyperaccumulation armours plants with direct defences against pathogens. In the present study, it was found that high leaf Mn concentrations (<2500 µg g(-1)) induced grapevine resistance to powdery mildew [Uncinula necator (Schw.) Burr]. Manganese delayed pathogen spreading after...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/ers175
更新日期:2012-09-01 00:00:00
abstract::Small heat shock proteins are a diverse, ancient, and important family of proteins. All organisms possess small heat shock proteins (sHSPs), indicating that these proteins evolved very early in the history of life prior to the divergence of the three domains of life (Archaea, Bacteria, and Eukarya). Comparing the stru...
journal_title:Journal of experimental botany
pub_type: 杂志文章,评审
doi:10.1093/jxb/ers355
更新日期:2013-01-01 00:00:00
abstract::Exposure to high ambient levels of NaCl affects plant water relations and creates ionic stress in the form of the cellular accumulation of Cl- and, in particular, Na+ ions. However, salt stress also impacts heavily on the homeostasis of other ions such as Ca2+, K+, and NO(3)(-) and therefore requires insights into how...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erj001
更新日期:2006-01-01 00:00:00
abstract::Inefficient use of phosphorus (P) in agriculture adds to production costs, increases the risk of eutrophication of waterways, and contributes to the rapid depletion of the world's non-renewable rock phosphate supplies. The removal of large quantities of P from fields in harvested grains is a major driver in the global...
journal_title:Journal of experimental botany
pub_type: 杂志文章,评审
doi:10.1093/jxb/erv517
更新日期:2016-03-01 00:00:00
abstract::Increasing leaf transpiration efficiency (TE) may provide leads for growing rice like dryland cereals such as wheat (Triticum aestivum). To explore avenues for improving TE in rice, variations in stomatal conductance (gs) and mesophyll conductance (gm) and their anatomical determinants were evaluated in two cultivars ...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erx314
更新日期:2017-11-02 00:00:00
abstract::One crucial problem that plants faced during their evolution, particularly during the transition to growth on land, was how to transport water, nutrients, metabolites, and small signaling molecules within a large, multicellular body. As a solution to this problem, land plants developed specific tissues for conducting ...
journal_title:Journal of experimental botany
pub_type: 杂志文章,评审
doi:10.1093/jxb/erw473
更新日期:2017-01-01 00:00:00
abstract::Endosperm cap (CAP) weakening and embryo elongation growth are prerequisites for the completion of lettuce seed germination. Although it has been proposed that the cell wall loosening underlying these processes results from an enzymatic mechanism, it is still unclear which enzymes are involved. Here it is shown that r...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/eru167
更新日期:2014-07-01 00:00:00
abstract::Teosinte branched1/cycloidea/proliferating (TCP) transcription factors play a broad role in plant growth and development, but their involvement in the regulation of anthocyanin biosynthesis is currently unclear. In this study, anthocyanin biosynthesis induced by different light intensities in apple (Malus domestica) w...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/eraa056
更新日期:2020-05-30 00:00:00
abstract::Plants have fundamental dependences on nitrogen and sulfur and frequently have to cope with chronic limitations when their supply is sub-optimal. This study aimed at characterizing the metabolomic, proteomic, and transcriptomic changes occurring in Arabidopsis leaves under chronic nitrate (Low-N) and chronic sulfate (...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/eraa337
更新日期:2020-10-22 00:00:00
abstract::Phototropin1 (phot1) perceives low- to high-fluence blue light stimuli and mediates both the first and second positive phototropisms. High-fluence blue light is known to induce autophosphorylation of phot1, leading to the second positive phototropism. However, the phosphorylation status of phot1 by low-fluence blue li...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erz344
更新日期:2019-10-24 00:00:00
abstract::The eukaryotic actin cytoskeleton is a highly dynamic framework that is involved in many biological processes, such as cell growth, division, morphology, and motility. G-actin polymerizes into microfilaments that associate into bundles, patches, and networks, which, in turn, organize into higher order structures that ...
journal_title:Journal of experimental botany
pub_type: 杂志文章,评审
doi:10.1093/jxb/erx195
更新日期:2017-06-15 00:00:00
abstract::Successful sexual reproduction often relies on the ability of plants to recognize self- or genetically-related pollen and prevent pollen tube growth soon after germination in order to avoid self-fertilization. Angiosperms have developed different reproductive barriers, one of the most extended being self-incompatibili...
journal_title:Journal of experimental botany
pub_type: 杂志文章,评审
doi:10.1093/jxb/erv083
更新日期:2015-05-01 00:00:00
abstract::A powerful technique is described to localize the activities of a range of enzymes in a wide variety of plant tissues. The method is based on the coupling of the enzymatic reaction to the reduction of NAD and subsequent reduction and precipitation of nitroblue tetrazolium. Enzymes that did not reduce NAD could be visu...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jexbot/53.367.361
更新日期:2002-02-01 00:00:00
abstract::We previously identified the W-box-like-4 (Wbl-4) element (GTAGTGACTCAT), one of six Wbl elements in the BjC-P promoter of the unusual chitinase gene BjCHI1 from Brassica juncea, as the core element responsive to fungal infection. Here, we report the isolation and characterization of the cognate transcription factor i...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erw240
更新日期:2016-08-01 00:00:00
abstract::Plants respond to many environmental changes by rapidly adjusting their hydraulic conductivity and transpiration rate, thereby optimizing water-use efficiency and preventing damage due to low water potential. A multiple-load-cell apparatus, time-series analysis of the measured data, and residual low-pass filtering met...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erq168
更新日期:2010-07-01 00:00:00
abstract::Phospholipid signaling plays an important role in plant immune responses against phytopathogenic bacteria in Nicotiana benthamiana. Here, we isolated two phospholipase C2 (PLC2) orthologs in the N. benthamiana genome, designated as PLC2-1 and 2-2. Both NbPLC2-1 and NbPLC2-2 were expressed in most tissues and were indu...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/eraa233
更新日期:2020-08-06 00:00:00
abstract::The determinism of bud bursting pattern along the 1-year-old shoot was studied at the molecular and morphological levels in the apple tree variety 'Lodi' which shows an acrotonic tendency. At the molecular level, the expression of KNAP2, which belongs to the class I KN1-like gene family, was studied. Measurements were...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erf063
更新日期:2002-11-01 00:00:00
abstract::Auxin is an essential regulator of plant growth and development, and auxin signaling components are conserved among land plants. Yet, a remarkable degree of natural variation in physiological and transcriptional auxin responses has been described among Arabidopsis thaliana accessions. As intraspecies comparisons offer...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erw457
更新日期:2017-01-01 00:00:00
abstract::Many plant species and/or genotypes are highly recalcitrant to Agrobacterium-mediated genetic transformation, and yet little is known about this phenomenon. Using several Arabidopsis genotypes/ecotypes, the results of this study indicated that phytohormone pretreatment could overcome this recalcitrance by increasing t...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jexbot/51.353.1961
更新日期:2000-12-01 00:00:00
abstract::Flavonoids are plant pigments that provide health benefits for human and animal consumers. Understanding why domesticated crops have altered pigmentation patterns and unraveling the molecular/genetic mechanisms that underlie this will facilitate the breeding of new (healthier) varieties. We present an overview of chan...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erz141
更新日期:2019-08-07 00:00:00
abstract::In recent years, efforts to exploit sunlight, a free and abundant energy source, have sped up dramatically. Oxygenic photosynthetic organisms, such as higher plants, algae, and cyanobacteria, can convert solar energy into chemical energy very efficiently using water as an electron donor. By providing organic building ...
journal_title:Journal of experimental botany
pub_type: 杂志文章,评审
doi:10.1093/jxb/erz054
更新日期:2019-03-27 00:00:00
abstract::Low-temperature stress is an important factor affecting the growth and development of rice (Oryza sativa L.) in temperate and high-elevation areas. Cold stress may cause various seedling injuries, delayed heading and yield reduction due to spikelet sterility. In this study, 181 microsatellite marker loci were used to ...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erg243
更新日期:2003-11-01 00:00:00
abstract::Theobroma cacao (cacao) is cultivated in tropical climates and is exposed to drought stress. The impact of the endophytic fungus Trichoderma hamatum isolate DIS 219b on cacao's response to drought was studied. Colonization by DIS 219b delayed drought-induced changes in stomatal conductance, net photosynthesis, and gre...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erp165
更新日期:2009-01-01 00:00:00
abstract::Iron is an essential element for all living organisms due to its ubiquitous role in redox and other enzymes, especially in the context of respiration and photosynthesis. The iron uptake and storage systems of terrestrial/higher plants are now reasonably well understood, with two basic strategies for iron uptake being ...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/ers225
更新日期:2012-10-01 00:00:00
abstract::During the Green Revolution, substantial increases in wheat (Triticum aestivum) yields were realized, at least in part, through the introduction of the Reduced height (Rht)-B1b and Rht-D1b semi-dwarfing alleles. In contrast to Rht-B1b and Rht-D1b, the Rht-B1c allele is characterized by extreme dwarfism and exceptional...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erw471
更新日期:2017-01-01 00:00:00
abstract::While many C4 lineages have Kranz anatomy around individual veins, Salsoleae have evolved the Salsoloid Kranz anatomy where a continuous dual layer of chlorenchyma cells encloses the vascular and water-storage tissue. With the aim of elucidating the evolution of C4 photosynthesis in Salsoleae, a broadly sampled molecu...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erw432
更新日期:2017-01-01 00:00:00
abstract::The plant root system is important for the uptake of water and nutrients and the anchoring of plants in the soil. Lateral roots (LRs) contribute considerably to root system architecture. Their post-embryonic formation is regulated by hormones and environmental cues. The hormone cytokinin influences LR formation and gr...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/ert291
更新日期:2013-11-01 00:00:00