In situ staining of activities of enzymes involved in carbohydrate metabolism in plant tissues.

abstract：:During grain filling in barley (Hordeum vulgare L. cv. Barke) reserves are remobilized from vegetative organs. Glumes represent the vegetative tissues closest to grains, senesce late, and are involved in the conversion of assimilates. To analyse glume development and metabolism related to grain filling, parallel trans...

journal_title：Journal of experimental botany

authors： Kohl S,Hollmann J,Erban A,Kopka J,Riewe D,Weschke W,Weber H

更新日期：2015-03-01 00:00:00

abstract：:Low temperature affects seed germination in plants, and low-temperature germination (LTG) is an important agronomic trait. Natural variation of LTG has been reported in rice, but the molecular basis for this variation is largely unknown. Here we report the phenotypic analysis of LTG in 187 rice natural accessions and ...

journal_title：Journal of experimental botany

authors： Wang X,Zou B,Shao Q,Cui Y,Lu S,Zhang Y,Huang Q,Huang J,Hua J

更新日期：2018-01-23 00:00:00

abstract：:An investigation aimed at a better understanding of the molecular adaptation mechanisms of salt stress was carried out in 7-d-old tomato Solanum lycopersicum (L.) Mill cultivars Patio and 'F144', using a proteomic approach. Total proteins were extracted from radicles and hypocotyls collected from both non-saline contr...

journal_title：Journal of experimental botany

authors： Chen S,Gollop N,Heuer B

更新日期：2009-01-01 00:00:00

abstract：:We previously reported that a novel motor protein belonging to the kinesin-12 family, NtKRP, displays critical roles in regulating embryo and seed size establishment. However, it remains unknown exactly how NtKRP contributes to this developmental process. Here, we report that a 60S ribosomal protein NtRPL17 directly i...

journal_title：Journal of experimental botany

authors： Tian S,Wu J,Liu Y,Huang X,Li F,Wang Z,Sun MX

更新日期：2017-11-28 00:00:00

abstract：:A model of kiwifruit berry development is presented, building on the model of Fishman and Génard used for peach fruit. That model has been extended to incorporate a number of important features of kiwifruit growth. First, the kiwifruit berry is attached to the stem through a pedicel/receptacle complex which contribute...

journal_title：Journal of experimental botany

authors： Hall AJ,Minchin PE,Clearwater MJ,Génard M

更新日期：2013-12-01 00:00:00

abstract：:Changes of stem diameter were continuously monitored during winter in two field-grown poplar clones, using automatic point dendrometers. The objective of this study was to find an analytical solution to seasonal synchronization of stem diameter oscillations and low air temperatures. The study identified to what extent...

journal_title：Journal of experimental botany

authors： Cocozza C,Lasserre B,Giovannelli A,Castro G,Fragnelli G,Tognetti R

更新日期：2009-01-01 00:00:00

abstract：:Plants of Lolium perenne L. were grown in sterile solution culture supplied with 2 mol m(-3) nitrogen as either nitrate or ammonium. Glutamine at 5 mol m(-3) was added to the nutrient solution of half the plants for 24 h. Root nitrate influx (at external nitrate concentrations 0-2000 mmol m(-3)) and amino acid concent...

journal_title：Journal of experimental botany

authors： Thornton B

更新日期：2004-03-01 00:00:00

abstract：:A suppressor screen using dwarf mutants of barley (Hordeum vulgare L.) led to the isolation of 'overgrowth' derivatives, which retained the original dwarfing gene but grew at a faster rate because of a new mutation. The new mutations were in the Slender1 (Sln1) gene (11/13 cases), which encodes the DELLA protein centr...

journal_title：Journal of experimental botany

authors： Chandler PM,Harding CA

更新日期：2013-04-01 00:00:00

abstract：:The gene encoding stilbene synthase (STS) plays a central role in many biochemical and physiological actions, and its metabolite resveratrol possesses broad-spectrum resistance to pathogens, as well as diverse pharmacological properties, notably an anticancer effect. Here, we report the expression analysis of the gene...

journal_title：Journal of experimental botany

authors： Xu W,Yu Y,Zhou Q,Ding J,Dai L,Xie X,Xu Y,Zhang C,Wang Y

更新日期：2011-05-01 00:00:00

abstract：:Leghemoglobins transport and deliver O2 to the symbiosomes inside legume nodules and are essential for nitrogen fixation. However, the roles of other hemoglobins (Hbs) in the rhizobia-legume symbiosis are unclear. Several Lotus japonicus mutants affecting LjGlb1-1, a non-symbiotic class 1 Hb, have been used to study t...

journal_title：Journal of experimental botany

authors： Fukudome M,Calvo-Begueria L,Kado T,Osuki K,Rubio MC,Murakami E,Nagata M,Kucho K,Sandal N,Stougaard J,Becana M,Uchiumi T

更新日期：2016-09-01 00:00:00

abstract：:Accounting for almost one-fifth of the primary production on Earth, the unicellular eukaryotic group of diatoms plays a key ecological and biogeochemical role in our contemporary oceans. Furthermore, as producers of various lipids and pigments, and characterized by their finely ornamented silica cell wall, diatoms hol...

journal_title：Journal of experimental botany

authors： Huysman MJ,Vyverman W,De Veylder L

更新日期：2014-06-01 00:00:00

abstract：:Plant hormones, in addition to regulating growth and development, are involved in biotic and abiotic stress responses. To investigate whether a hormone signalling pathway plays a role in the plant response to the heavy metal cadmium (Cd), gene expression data in response to eight hormone treatments were retrieved from...

journal_title：Journal of experimental botany

authors： Villiers F,Jourdain A,Bastien O,Leonhardt N,Fujioka S,Tichtincky G,Parcy F,Bourguignon J,Hugouvieux V

更新日期：2012-02-01 00:00:00

abstract：:Because it has a high demand for sulphur (S), oilseed rape is particularly sensitive to S limitation. However, the physiological effects of S limitation remain unclear, especially during the rosette stage. For this reason a study was conducted to determine the effects of mineral S limitation on nitrogen (N) and S upta...

journal_title：Journal of experimental botany

authors： Abdallah M,Dubousset L,Meuriot F,Etienne P,Avice JC,Ourry A

更新日期：2010-06-01 00:00:00

abstract：:The spatial and temporal distribution of expression of two cytosolic members of the AtHsp90 gene family was assessed during early development. In stressed transgenic plants bearing the AtHsp90-3 promoter, beta-glucuronidase (GUS) activity was strong in meristematic tissues. Expression was also detected in vascular tis...

journal_title：Journal of experimental botany

authors： Prasinos C,Krampis K,Samakovli D,Hatzopoulos P

更新日期：2005-02-01 00:00:00

abstract：:Realistic experimental protocols to screen for drought adaptation in controlled conditions are crucial if high throughput phenotyping is to be used for the identification of high performance lines, and is especially important in the evaluation of transgenes where stringent biosecurity measures restrict the frequency o...

journal_title：Journal of experimental botany

authors： Saint Pierre C,Crossa JL,Bonnett D,Yamaguchi-Shinozaki K,Reynolds MP

更新日期：2012-03-01 00:00:00

abstract：:Theobroma cacao (cacao) is cultivated in tropical climates and is exposed to drought stress. The impact of the endophytic fungus Trichoderma hamatum isolate DIS 219b on cacao's response to drought was studied. Colonization by DIS 219b delayed drought-induced changes in stomatal conductance, net photosynthesis, and gre...

journal_title：Journal of experimental botany

authors： Bae H,Sicher RC,Kim MS,Kim SH,Strem MD,Melnick RL,Bailey BA

更新日期：2009-01-01 00:00:00

abstract：:Seed size is a key determinant of evolutionary fitness, and is also one of the most important components of seed yield. In angiosperms, seed development begins with double fertilization, which leads to the formation of a diploid embryo and a triploid endosperm. The outermost layer of the seed is the seed coat, which d...

journal_title：Journal of experimental botany

authors： Li N,Li Y

更新日期：2015-02-01 00:00:00

abstract：:Among 50 CLE gene family members in the Populus trichocarpa genome, three and six PtCLE genes encode a CLE motif sequence highly homologous to Arabidopsis CLV3 and TDIF peptides, respectively, which potentially make them functional equivalents. To test and compare their biological activity, we first chemically synthes...

journal_title：Journal of experimental botany

authors： Liu Y,Yang S,Song Y,Men S,Wang J

更新日期：2016-04-01 00:00:00

abstract：:Recovery of photosynthesis in rehydrating desiccated leaves of the poikilochlorophyllous desiccation-tolerant plant Xerophyta scabrida was investigated. Detached leaves were remoistened under 12 h light/dark cycles for 96 h. Water, chlorophyll (Chl), and protein contents, Chl fluorescence, photosynthesis-CO(2) concent...

journal_title：Journal of experimental botany

authors： Pérez P,Rabnecz G,Laufer Z,Gutiérrez D,Tuba Z,Martínez-Carrasco R

更新日期：2011-01-01 00:00:00

abstract：:The role of abscisic acid (ABA) in the weakening of the endosperm cap prior to radicle protrusion in tomato (Lycopersicon esculentum Mill. cv. Moneymaker) seeds was studied. The endosperm cap weakened substantially in both water and ABA during the first 38 h of imbibition. After 38 h the force required for endosperm c...

journal_title：Journal of experimental botany

authors： Toorop PE,van Aelst AC,Hilhorst HW

更新日期：2000-08-01 00:00:00

abstract：:An enzymatically active recombinant protein kinase, previously isolated and characterized in Fagus sylvatica L. dormant seeds (FsPK1), was used to obtain a specific polyclonal antibody against this protein. Immunoblotting and immunohistochemical analysis of FsPK1 protein in beech seeds showed a strong immunostaining i...

journal_title：Journal of experimental botany

authors： Reyes D,Rodríguez D,Lorenzo O,Nicolás G,Cañas R,Cantón FR,Canovas FM,Nicolás C

更新日期：2006-01-01 00:00:00

abstract：:The stamen produces pollen grains for pollination in higher plants. Coordinated development of four microsporangia in the stamen is essential for normal fertility. The roles of miR165/166-directed pathways in the establishment of adaxial-abaxial polarity have been well defined in leaves. However, the molecular mechani...

journal_title：Journal of experimental botany

authors： Lian H,Li X,Liu Z,He Y

更新日期：2013-08-01 00:00:00

abstract：:The impact of sulphur limitation on the remobilization of endogenous S compounds during the rosette stage of oilseed rape, and the interactions with N availability on these processes, were examined using a long-term (34)SO(4)(2-) labelling method combined with a study of leaf senescence progression (using SAG12/Cab as...

journal_title：Journal of experimental botany

authors： Dubousset L,Abdallah M,Desfeux AS,Etienne P,Meuriot F,Hawkesford MJ,Gombert J,Ségura R,Bataillé MP,Rezé S,Bonnefoy J,Ameline AF,Ourry A,Le Dily F,Avice JC

更新日期：2009-01-01 00:00:00

abstract：:Phosphorus is an important macronutrient for plant growth, but often deficient in soil. To understand the molecular basis of the complex responses of potato (Solanum tuberosum L.) to phosphate (Pi) deficiency stress, the RNA-Seq approach was taken to identify genes responding to Pi starvation in potato roots. A total ...

journal_title：Journal of experimental botany

authors： Zhou X,Zha M,Huang J,Li L,Imran M,Zhang C

更新日期：2017-02-01 00:00:00

abstract：:Abiotic factors inducing osmotic stress can influence the plant immune response and resistance to pathogen infections. In this study, the effect of polyethylene glycol (PEG)- and sucrose-induced osmotic stress on polyamine (PA) homeostasis and the basal immune response in grapevine plantlets before and after Botrytis ...

journal_title：Journal of experimental botany

authors： Hatmi S,Trotel-Aziz P,Villaume S,Couderchet M,Clément C,Aziz A

更新日期：2014-01-01 00:00:00

abstract：:Cultivated varieties generally differ greatly from wild genotypes of the same closely related species. However, the processes responsible for these differences have not been elucidated. To analyse variations in fruit mass, fruit growth was characterized in a peach cultivar, a wild related species non-cultivated, and f...

journal_title：Journal of experimental botany

authors： Quilot B,Génard M,Kervella J,Lescourret F

更新日期：2002-07-01 00:00:00

abstract：:Soybean seeds provide an excellent source of protein for human and livestock nutrition. However, their nutritional quality is hampered by a low concentration of the essential sulfur amino acid, methionine (Met). In order to study factors that regulate Met synthesis in soybean seeds, this study used the Met-insensitive...

journal_title：Journal of experimental botany

authors： Song S,Hou W,Godo I,Wu C,Yu Y,Matityahu I,Hacham Y,Sun S,Han T,Amir R

更新日期：2013-04-01 00:00:00

abstract：:Cell wall apposition (CWA) formation is one of the first lines of defence used by plants to halt invading fungi such as powdery mildew. Lignin is a complex polymer of hydroxylated and methoxylated phenylpropane units (monolignols) and lignification renders the cell wall more resistant to pathogen attack. The role of m...

journal_title：Journal of experimental botany

authors： Bhuiyan NH,Selvaraj G,Wei Y,King J

更新日期：2009-01-01 00:00:00

abstract：:The hormone ethylene regulates a wide range of plant developmental processes and EBF (EIN3-binding F-box) proteins were shown to negatively regulate the ethylene signalling pathway via mediating the degradation of EIN3/EIL proteins. The present study reports on the identification of two tomato F-box genes, Sl-EBF1 and...

journal_title：Journal of experimental botany

authors： Yang Y,Wu Y,Pirrello J,Regad F,Bouzayen M,Deng W,Li Z

更新日期：2010-03-01 00:00:00

abstract：:Apoplastic transport barriers in the roots of rice (Oryza sativa L. cv. IR64) and corn (Zea mays L. cv. Helix) were isolated enzymatically. Following chemical degradation (monomerization, derivatization), the amounts of aliphatic and aromatic suberin monomers were analysed quantitatively by gas chromatography and mass...

journal_title：Journal of experimental botany

authors： Schreiber L,Franke R,Hartmann KD,Ranathunge K,Steudle E

更新日期：2005-05-01 00:00:00